|Institutional Source||Beutler Lab|
|Gene Name||wingless-type MMTV integration site family, member 10A|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R7278 (G1)|
|Chromosomal Location||74791516-74804179 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 74793482 bp|
|Amino Acid Change||Histidine to Leucine at position 78 (H78L)|
|Ref Sequence||ENSEMBL: ENSMUSP00000006718 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000006718]|
|Predicted Effect||possibly damaging
AA Change: H78L
PolyPhen 2 Score 0.915 (Sensitivity: 0.81; Specificity: 0.94)
AA Change: H78L
|Coding Region Coverage||
|Validation Efficiency||99% (78/79)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is strongly expressed in the cell lines of promyelocytic leukemia and Burkitt's lymphoma. In addition, it and another family member, the WNT6 gene, are strongly coexpressed in colorectal cancer cell lines. The gene overexpression may play key roles in carcinogenesis through activation of the WNT-beta-catenin-TCF signaling pathway. This gene and the WNT6 gene are clustered in the chromosome 2q35 region. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit taurodontism, supernumerary molars, small molars and misshapened crowns. Mice homozygous for a conditional allele activated in Krt14+ cells also exhibit decreased basal cell proliferation affecting the tongue, sweat glands and nails. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Wnt10a||
(F):5'- GGAAGAGTGATTACGGCCTGTG -3'
(R):5'- AGCTAGCAAGTTTGGGATGG -3'
(F):5'- GTGTGTTATTAAACAGGCCCTCACAG -3'
(R):5'- AGCAAGTTTGGGATGGGGGTG -3'