Incidental Mutation 'R7372:Kif3c'
ID572172
Institutional Source Beutler Lab
Gene Symbol Kif3c
Ensembl Gene ENSMUSG00000020668
Gene Namekinesin family member 3C
SynonymsN-4 kinesin
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.220) question?
Stock #R7372 (G1)
Quality Score225.009
Status Validated
Chromosome12
Chromosomal Location3365132-3406494 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to A at 3387592 bp
ZygosityHeterozygous
Amino Acid Change Methionine to Lysine at position 531 (M531K)
Ref Sequence ENSEMBL: ENSMUSP00000020999 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000020999] [ENSMUST00000219895] [ENSMUST00000220210]
Predicted Effect probably benign
Transcript: ENSMUST00000020999
AA Change: M531K

PolyPhen 2 Score 0.028 (Sensitivity: 0.95; Specificity: 0.81)
SMART Domains Protein: ENSMUSP00000020999
Gene: ENSMUSG00000020668
AA Change: M531K

DomainStartEndE-ValueType
KISc 8 375 5.43e-171 SMART
low complexity region 404 421 N/A INTRINSIC
low complexity region 435 443 N/A INTRINSIC
low complexity region 486 505 N/A INTRINSIC
Blast:KISc 508 579 3e-8 BLAST
low complexity region 580 602 N/A INTRINSIC
Blast:KISc 603 666 1e-16 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000219895
AA Change: M13K

PolyPhen 2 Score 0.028 (Sensitivity: 0.95; Specificity: 0.81)
Predicted Effect probably benign
Transcript: ENSMUST00000220210
AA Change: M531K

PolyPhen 2 Score 0.028 (Sensitivity: 0.95; Specificity: 0.81)
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.2%
Validation Efficiency 99% (68/69)
MGI Phenotype PHENOTYPE: Mice homozygous for disruptions in this gene display a normal phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abl2 G A 1: 156,622,619 V119M probably damaging Het
Acss2 T C 2: 155,557,180 V454A probably damaging Het
Adam6b T A 12: 113,490,164 D200E probably benign Het
Adarb1 T A 10: 77,295,878 probably null Het
Aqp12 C T 1: 93,006,366 probably benign Het
Atrnl1 T C 19: 57,935,646 V1281A possibly damaging Het
Brdt A G 5: 107,370,294 E761G possibly damaging Het
Bsn A T 9: 108,110,519 I2678N unknown Het
C2cd2 A T 16: 97,875,380 C136S Het
Camk4 A G 18: 33,185,125 D445G probably benign Het
Cckar T C 5: 53,707,282 T26A probably damaging Het
Cd209a T A 8: 3,748,857 probably null Het
Cept1 A G 3: 106,503,740 F379S probably benign Het
Crhr1 A G 11: 104,163,893 probably null Het
Cryzl1 T C 16: 91,712,197 E72G probably benign Het
Ctnnal1 T A 4: 56,826,285 E526V possibly damaging Het
Cyp3a41a T C 5: 145,713,564 I90V possibly damaging Het
Dek T C 13: 47,105,577 E51G unknown Het
Evc2 T A 5: 37,387,133 V742E probably damaging Het
Fat4 G A 3: 38,890,209 V1084M probably damaging Het
Fscb G A 12: 64,471,824 T956I unknown Het
Glipr2 T C 4: 43,968,184 L29P probably damaging Het
Gm19402 A T 10: 77,690,427 S111T unknown Het
Gm340 T C 19: 41,585,506 L900P probably damaging Het
Gm4553 ACCCTTGCAGCCACCACAGGAGCCACAGCCCCCACAGGAGCTACAGCCTCCCTTGCAGCCACCACAGGAGCCACAGCCCCCACAGGAGCTACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCC ACCCTTGCAGCCACCACAGGAGCCACAGCCCCCACAGGAGCTACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCC 7: 142,165,420 probably benign Het
Gnl3 T C 14: 31,016,886 K115E probably benign Het
Gpn1 T A 5: 31,501,121 F147I probably damaging Het
Gsdmd T A 15: 75,865,769 L232H probably benign Het
Helz2 A G 2: 181,238,423 V500A possibly damaging Het
Hemk1 T C 9: 107,337,068 E55G probably benign Het
Hs2st1 A T 3: 144,435,460 probably null Het
Ighv1-63 A G 12: 115,495,866 V37A probably damaging Het
Iqsec3 C A 6: 121,384,032 E956* probably null Het
Kcnh8 T A 17: 52,894,101 I521K probably damaging Het
Kif5c T A 2: 49,758,659 probably null Het
Krt15 A T 11: 100,135,560 V100E possibly damaging Het
Lrrc32 A G 7: 98,499,807 D598G probably benign Het
Mug2 T C 6: 122,083,466 V1387A possibly damaging Het
Nin T A 12: 70,056,029 E275V Het
Olfr493 A G 7: 108,346,496 F162L probably benign Het
Papolg A G 11: 23,866,439 I698T probably benign Het
Pcdhb20 A G 18: 37,506,787 N789D probably benign Het
Pih1d3 A T 1: 31,223,351 D138V probably damaging Het
Pik3cg C T 12: 32,197,197 M842I probably damaging Het
Pnn T A 12: 59,068,979 D135E probably damaging Het
Pold1 C A 7: 44,543,423 R5L possibly damaging Het
Prl8a1 A T 13: 27,574,106 F207I probably damaging Het
Prr11 A G 11: 87,098,774 V257A probably benign Het
Prtg C T 9: 72,851,566 R401* probably null Het
Rasgrp1 A G 2: 117,285,154 M651T probably benign Het
Ryr2 T A 13: 11,680,999 H2994L probably damaging Het
Snx13 A T 12: 35,078,951 I23L probably benign Het
Snx7 A G 3: 117,782,351 L429P probably damaging Het
Spta1 C T 1: 174,197,635 Q689* probably null Het
Spty2d1 T C 7: 46,998,944 D79G probably damaging Het
Tbc1d9b A G 11: 50,168,688 probably null Het
Tnxb T C 17: 34,717,254 F2722L possibly damaging Het
Tppp2 C T 14: 51,919,408 R81C probably benign Het
Trim6 G T 7: 104,232,636 A391S probably benign Het
Trim69 A C 2: 122,178,583 T375P possibly damaging Het
Trim72 A G 7: 128,004,686 N68S possibly damaging Het
Trrap G A 5: 144,789,398 V386I probably benign Het
Ttn T C 2: 76,947,931 I1371V unknown Het
Usp17lb A T 7: 104,841,706 probably null Het
Vmn2r20 A T 6: 123,385,509 L772Q probably damaging Het
Ypel3 A G 7: 126,780,028 E91G probably benign Het
Zbtb21 C T 16: 97,950,369 E905K possibly damaging Het
Zfp189 T A 4: 49,530,417 C507S possibly damaging Het
Other mutations in Kif3c
AlleleSourceChrCoordTypePredicted EffectPPH Score
R0049:Kif3c UTSW 12 3367090 missense possibly damaging 0.91
R0049:Kif3c UTSW 12 3367090 missense possibly damaging 0.91
R0189:Kif3c UTSW 12 3365989 missense probably benign 0.10
R0727:Kif3c UTSW 12 3366776 missense probably benign 0.01
R0885:Kif3c UTSW 12 3365981 start codon destroyed probably benign 0.00
R1796:Kif3c UTSW 12 3367299 missense probably benign 0.01
R2229:Kif3c UTSW 12 3366671 missense probably benign 0.01
R4728:Kif3c UTSW 12 3365873 start gained probably benign
R4870:Kif3c UTSW 12 3401735 missense probably damaging 1.00
R5586:Kif3c UTSW 12 3389656 missense probably benign 0.41
R5662:Kif3c UTSW 12 3367031 missense probably damaging 0.99
R6969:Kif3c UTSW 12 3366114 missense probably benign 0.30
R7216:Kif3c UTSW 12 3366126 missense probably benign 0.06
R7533:Kif3c UTSW 12 3366510 missense probably damaging 1.00
R8373:Kif3c UTSW 12 3366089 missense probably benign 0.44
X0052:Kif3c UTSW 12 3367027 missense probably benign 0.30
X0064:Kif3c UTSW 12 3366868 missense probably damaging 1.00
Z1177:Kif3c UTSW 12 3367245 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- AGCACTGCTGTTCTCCACTG -3'
(R):5'- AGGGGAATCTACCACTTCCTC -3'

Sequencing Primer
(F):5'- TTCTCCACTGCAGAGAAGGTTTAAGG -3'
(R):5'- AGAGAGACCCCGAGCTGGTAC -3'
Posted On2019-09-13