Mutagenetix > Incidental Mutation

Incidental Mutation 'R7374:Vmn2r74'

572273

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r74

Ensembl Gene

ENSMUSG00000090774

Gene Name

vomeronasal 2, receptor 74

Synonyms

EG546980

MMRRC Submission

045457-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.105) question?

Stock #

R7374 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

85601075-85610690 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85606630 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 239 (I239V)

Ref Sequence

ENSEMBL: ENSMUSP00000126917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166355]

AlphaFold

E9PW21

Predicted Effect

probably benign
Transcript: ENSMUST00000166355
AA Change: I239V

PolyPhen 2 Score 0.017 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000126917
Gene: ENSMUSG00000090774
AA Change: I239V

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	467	7.3e-28	PFAM
Pfam:NCD3G	510	562	4.7e-20	PFAM
Pfam:7tm_3	592	830	1.3e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	C	T	11: 9,242,136 (GRCm39)	T1333I	possibly damaging	Het
Abcd4	A	T	12: 84,653,017 (GRCm39)	L395*	probably null	Het
Cdh23	G	A	10: 60,153,679 (GRCm39)	R2304W	probably damaging	Het
Ckap2l	T	C	2: 129,126,883 (GRCm39)	T432A	probably damaging	Het
Cplane1	T	A	15: 8,276,731 (GRCm39)	W2792R	unknown	Het
Cyp2c68	T	C	19: 39,727,648 (GRCm39)		probably null	Het
Ddx51	C	A	5: 110,804,998 (GRCm39)	T580K	probably damaging	Het
Fbxo44	T	A	4: 148,241,094 (GRCm39)	D112V	probably benign	Het
Ffar2	T	C	7: 30,519,465 (GRCm39)	N25S	probably damaging	Het
Gas6	A	T	8: 13,524,802 (GRCm39)	V330D	probably damaging	Het
Gfpt1	A	G	6: 87,027,959 (GRCm39)	T22A	probably benign	Het
Gm3371	A	G	14: 44,641,240 (GRCm39)	V108A		Het
Gm8257	T	C	14: 44,887,740 (GRCm39)	E215G	probably benign	Het
Gria1	T	A	11: 57,080,634 (GRCm39)	S206T	probably benign	Het
Gria2	T	C	3: 80,648,383 (GRCm39)	T118A	probably benign	Het
Hfe	T	G	13: 23,890,030 (GRCm39)	T248P	probably damaging	Het
Kdm5d	T	C	Y: 941,491 (GRCm39)	W1231R	probably benign	Het
Ltbp2	C	T	12: 84,876,949 (GRCm39)	D419N	probably damaging	Het
Mei1	G	A	15: 81,980,109 (GRCm39)	A232T		Het
Mgam	G	A	6: 40,734,373 (GRCm39)	E1473K	possibly damaging	Het
Mtbp	A	C	15: 55,426,355 (GRCm39)	E99A	possibly damaging	Het
Muc5b	A	T	7: 141,416,863 (GRCm39)	T3270S	probably benign	Het
Myl2	A	G	5: 122,239,726 (GRCm39)	Y28C		Het
Nrxn1	A	G	17: 90,896,097 (GRCm39)		probably null	Het
Ntn4	A	G	10: 93,518,434 (GRCm39)	H247R	probably benign	Het
Numa1	T	C	7: 101,658,335 (GRCm39)	V1568A	probably benign	Het
Nyap1	C	T	5: 137,733,791 (GRCm39)	G414D	probably damaging	Het
Or5p68	A	G	7: 107,946,095 (GRCm39)	F31S	probably damaging	Het
Or8k25	C	A	2: 86,244,196 (GRCm39)	A67S	probably benign	Het
Pdgfrb	G	A	18: 61,204,780 (GRCm39)	V547I	possibly damaging	Het
Pom121l12	A	T	11: 14,549,962 (GRCm39)	M223L	probably benign	Het
Prps1l1	T	C	12: 35,035,424 (GRCm39)	S180P	possibly damaging	Het
Ptprf	A	G	4: 118,114,689 (GRCm39)	S189P	probably damaging	Het
Radil	G	A	5: 142,471,235 (GRCm39)	R1014W	probably damaging	Het
Rfx5	C	T	3: 94,866,053 (GRCm39)	P451S	unknown	Het
Rhbdl2	G	C	4: 123,711,658 (GRCm39)	V132L	probably benign	Het
Slc15a2	G	T	16: 36,572,207 (GRCm39)	T719K	probably benign	Het
Slc9a9	A	G	9: 94,937,542 (GRCm39)	T465A	possibly damaging	Het
Son	AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	16: 91,457,222 (GRCm39)		probably benign	Het
Sv2a	A	T	3: 96,095,525 (GRCm39)	Y306F	probably benign	Het
Sv2c	A	T	13: 96,125,644 (GRCm39)	H364Q	probably damaging	Het
Tmem181a	T	A	17: 6,354,533 (GRCm39)	L401Q	possibly damaging	Het
Trav9d-4	A	G	14: 53,221,300 (GRCm39)	Y98C	probably damaging	Het
Trim33	T	A	3: 103,217,639 (GRCm39)	C195S	probably damaging	Het
Uroc1	T	C	6: 90,315,815 (GRCm39)	Y191H	probably damaging	Het
Usp9y	T	C	Y: 1,381,305 (GRCm39)	I839V	probably benign	Het
Vmn1r52	A	T	6: 90,156,118 (GRCm39)	I141F	probably benign	Het
Vmn1r59	T	C	7: 5,457,160 (GRCm39)	H200R	probably damaging	Het
Vmn2r68	A	T	7: 84,881,607 (GRCm39)	M491K	possibly damaging	Het
Wrn	A	C	8: 33,758,939 (GRCm39)	C1006W	probably damaging	Het

Other mutations in Vmn2r74

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00899:Vmn2r74	APN	7	85,606,338 (GRCm39)	missense	probably benign	0.03
IGL00904:Vmn2r74	APN	7	85,606,788 (GRCm39)	missense	probably benign	0.05
IGL01285:Vmn2r74	APN	7	85,606,692 (GRCm39)	missense	possibly damaging	0.54
IGL01300:Vmn2r74	APN	7	85,606,414 (GRCm39)	missense	probably benign	0.00
IGL01410:Vmn2r74	APN	7	85,610,500 (GRCm39)	missense	possibly damaging	0.83
IGL01827:Vmn2r74	APN	7	85,606,800 (GRCm39)	missense	probably benign	0.00
IGL02094:Vmn2r74	APN	7	85,610,669 (GRCm39)	missense	probably benign	0.01
IGL02252:Vmn2r74	APN	7	85,606,531 (GRCm39)	missense	probably benign	0.41
IGL02349:Vmn2r74	APN	7	85,601,724 (GRCm39)	missense	probably damaging	0.99
IGL02438:Vmn2r74	APN	7	85,601,824 (GRCm39)	missense	probably damaging	0.98
IGL02554:Vmn2r74	APN	7	85,606,581 (GRCm39)	missense	probably benign	0.00
IGL03036:Vmn2r74	APN	7	85,601,900 (GRCm39)	nonsense	probably null
IGL03370:Vmn2r74	APN	7	85,607,265 (GRCm39)	missense	probably benign
R0115:Vmn2r74	UTSW	7	85,606,564 (GRCm39)	missense	probably benign	0.00
R0333:Vmn2r74	UTSW	7	85,601,491 (GRCm39)	missense	probably benign	0.06
R0415:Vmn2r74	UTSW	7	85,610,618 (GRCm39)	missense	probably damaging	1.00
R0571:Vmn2r74	UTSW	7	85,601,629 (GRCm39)	missense	probably damaging	1.00
R0626:Vmn2r74	UTSW	7	85,610,517 (GRCm39)	nonsense	probably null
R0659:Vmn2r74	UTSW	7	85,605,122 (GRCm39)	splice site	probably benign
R1202:Vmn2r74	UTSW	7	85,610,545 (GRCm39)	missense	possibly damaging	0.83
R1473:Vmn2r74	UTSW	7	85,610,618 (GRCm39)	missense	probably damaging	1.00
R1908:Vmn2r74	UTSW	7	85,601,650 (GRCm39)	missense	probably benign
R2079:Vmn2r74	UTSW	7	85,606,383 (GRCm39)	missense	probably benign	0.00
R2368:Vmn2r74	UTSW	7	85,610,522 (GRCm39)	missense	probably benign	0.39
R3782:Vmn2r74	UTSW	7	85,605,322 (GRCm39)	missense	probably benign	0.01
R3824:Vmn2r74	UTSW	7	85,607,466 (GRCm39)	missense	probably damaging	1.00
R3977:Vmn2r74	UTSW	7	85,607,345 (GRCm39)	missense	probably benign	0.01
R4182:Vmn2r74	UTSW	7	85,606,395 (GRCm39)	missense	possibly damaging	0.87
R4289:Vmn2r74	UTSW	7	85,606,562 (GRCm39)	missense	probably benign
R4294:Vmn2r74	UTSW	7	85,606,624 (GRCm39)	missense	probably benign	0.14
R4645:Vmn2r74	UTSW	7	85,606,317 (GRCm39)	missense	probably benign
R4646:Vmn2r74	UTSW	7	85,606,782 (GRCm39)	missense	probably benign	0.42
R4655:Vmn2r74	UTSW	7	85,610,555 (GRCm39)	missense	probably benign
R4901:Vmn2r74	UTSW	7	85,605,199 (GRCm39)	nonsense	probably null
R5532:Vmn2r74	UTSW	7	85,601,197 (GRCm39)	missense	probably benign	0.32
R5642:Vmn2r74	UTSW	7	85,606,588 (GRCm39)	missense	probably benign	0.00
R5913:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6035:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6035:Vmn2r74	UTSW	7	85,601,098 (GRCm39)	missense	probably damaging	0.98
R6039:Vmn2r74	UTSW	7	85,607,526 (GRCm39)	critical splice acceptor site	probably null
R6039:Vmn2r74	UTSW	7	85,607,526 (GRCm39)	critical splice acceptor site	probably null
R6170:Vmn2r74	UTSW	7	85,606,348 (GRCm39)	missense	probably benign	0.03
R6232:Vmn2r74	UTSW	7	85,607,498 (GRCm39)	missense	possibly damaging	0.82
R6238:Vmn2r74	UTSW	7	85,601,280 (GRCm39)	missense	probably damaging	1.00
R6255:Vmn2r74	UTSW	7	85,601,659 (GRCm39)	missense	possibly damaging	0.90
R6468:Vmn2r74	UTSW	7	85,610,599 (GRCm39)	missense	probably benign	0.34
R6732:Vmn2r74	UTSW	7	85,606,758 (GRCm39)	missense	probably damaging	1.00
R6816:Vmn2r74	UTSW	7	85,610,621 (GRCm39)	nonsense	probably null
R6836:Vmn2r74	UTSW	7	85,606,630 (GRCm39)	missense	probably benign	0.00
R6995:Vmn2r74	UTSW	7	85,606,860 (GRCm39)	critical splice acceptor site	probably null
R6995:Vmn2r74	UTSW	7	85,601,943 (GRCm39)	missense	probably benign	0.01
R7186:Vmn2r74	UTSW	7	85,601,150 (GRCm39)	nonsense	probably null
R7246:Vmn2r74	UTSW	7	85,605,173 (GRCm39)	missense	probably benign
R7505:Vmn2r74	UTSW	7	85,606,279 (GRCm39)	nonsense	probably null
R7525:Vmn2r74	UTSW	7	85,610,510 (GRCm39)	missense	probably benign
R7569:Vmn2r74	UTSW	7	85,601,544 (GRCm39)	missense	probably damaging	0.99
R7644:Vmn2r74	UTSW	7	85,606,746 (GRCm39)	missense	probably benign	0.11
R7956:Vmn2r74	UTSW	7	85,605,166 (GRCm39)	missense	probably benign	0.09
R8119:Vmn2r74	UTSW	7	85,610,690 (GRCm39)	start codon destroyed	probably null	0.08
R8131:Vmn2r74	UTSW	7	85,601,943 (GRCm39)	missense	probably benign	0.01
R8147:Vmn2r74	UTSW	7	85,605,227 (GRCm39)	nonsense	probably null
R8181:Vmn2r74	UTSW	7	85,605,324 (GRCm39)	missense	probably damaging	1.00
R8184:Vmn2r74	UTSW	7	85,601,454 (GRCm39)	missense	probably benign	0.00
R8375:Vmn2r74	UTSW	7	85,601,914 (GRCm39)	missense	possibly damaging	0.64
R8948:Vmn2r74	UTSW	7	85,606,569 (GRCm39)	missense	probably damaging	1.00
R8950:Vmn2r74	UTSW	7	85,606,569 (GRCm39)	missense	probably damaging	1.00
R9033:Vmn2r74	UTSW	7	85,606,414 (GRCm39)	missense	probably benign
R9342:Vmn2r74	UTSW	7	85,606,624 (GRCm39)	missense	probably benign	0.14
R9578:Vmn2r74	UTSW	7	85,606,101 (GRCm39)	missense	probably benign	0.01
R9607:Vmn2r74	UTSW	7	85,610,619 (GRCm39)	missense	probably benign	0.02
R9776:Vmn2r74	UTSW	7	85,605,212 (GRCm39)	missense	possibly damaging	0.70
Z1176:Vmn2r74	UTSW	7	85,604,835 (GRCm39)	missense	probably damaging	1.00
Z31818:Vmn2r74	UTSW	7	85,604,729 (GRCm39)	splice site	probably null

Predicted Primers

PCR Primer
(F):5'- AGTGGACGCATATAGCAAGAAATCTC -3'
(R):5'- TTCACCAGATATCTCGCAAGGAC -3'

Sequencing Primer
(F):5'- AGTTGTGATACGCTAACCCAG -3'
(R):5'- GATATCTCGCAAGGACACATTTC -3'

Posted On

2019-09-13