Mutagenetix > Incidental Mutation

Incidental Mutation 'R7378:Asxl1'

572439

Institutional Source

Beutler Lab

Gene Symbol

Asxl1

Ensembl Gene

ENSMUSG00000042548

Gene Name

ASXL transcriptional regulator 1

Synonyms

MMRRC Submission

045460-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7378 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

153187750-153245927 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 153243913 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Arginine at position 1489 (C1489R)

Ref Sequence

ENSEMBL: ENSMUSP00000105413 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109790] [ENSMUST00000227428]

AlphaFold

P59598

Predicted Effect

probably damaging
Transcript: ENSMUST00000109790
AA Change: C1489R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000105413
Gene: ENSMUSG00000042548
AA Change: C1489R

Domain	Start	End	E-Value	Type
Pfam:HARE-HTH	11	83	1.6e-20	PFAM
low complexity region	199	209	N/A	INTRINSIC
Pfam:ASXH	236	361	5.9e-40	PFAM
low complexity region	411	422	N/A	INTRINSIC
low complexity region	639	667	N/A	INTRINSIC
low complexity region	705	716	N/A	INTRINSIC
low complexity region	848	860	N/A	INTRINSIC
low complexity region	986	1000	N/A	INTRINSIC
Pfam:PHD_3	1446	1512	6.3e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000227428
AA Change: C1488R

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Meta Mutation Damage Score

0.9128

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

99% (138/140)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]
PHENOTYPE: Disruption of this gene causes alterations in lymphocyte development in adult mice. Mice homozygous for a different knock-out allele exhibit complete lethality. Mice heterozygous for this allele exhibit eye opacity and abnormal vertebrae morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 142 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	A	C	6: 128,523,210 (GRCm39)		probably null	Het
Aars1	A	G	8: 111,768,974 (GRCm39)	Y258C	probably damaging	Het
Acaa2	A	T	18: 74,938,943 (GRCm39)	Y376F	probably benign	Het
Adcy1	C	T	11: 7,119,543 (GRCm39)	S1062F	possibly damaging	Het
Adgrb3	A	T	1: 25,571,000 (GRCm39)	C492*	probably null	Het
Adgrg6	A	G	10: 14,411,636 (GRCm39)	F22S	probably benign	Het
Adh1	A	G	3: 137,994,648 (GRCm39)		probably null	Het
Afm	T	A	5: 90,699,259 (GRCm39)	C568S	probably benign	Het
Ago4	C	T	4: 126,405,257 (GRCm39)	E439K	probably benign	Het
Aifm2	T	G	10: 61,563,496 (GRCm39)	V122G	possibly damaging	Het
Akap6	A	G	12: 53,189,357 (GRCm39)	N2257S	probably benign	Het
Ap3m2	A	G	8: 23,294,026 (GRCm39)	I8T	probably benign	Het
Apc2	C	A	10: 80,147,228 (GRCm39)	L761I	probably damaging	Het
Asap2	A	G	12: 21,162,052 (GRCm39)	D3G	probably benign	Het
Atm	T	G	9: 53,364,737 (GRCm39)		probably null	Het
Bmal1	C	A	7: 112,898,415 (GRCm39)	T281K	probably benign	Het
Bpifa6	C	A	2: 153,828,353 (GRCm39)	A153E	probably damaging	Het
Cav2	A	G	6: 17,282,059 (GRCm39)	T106A	probably benign	Het
Cavin4	A	G	4: 48,663,631 (GRCm39)	N4D	probably benign	Het
Cenpc1	T	C	5: 86,194,358 (GRCm39)	N136S	probably benign	Het
Cfap45	T	A	1: 172,365,910 (GRCm39)		probably null	Het
Col3a1	G	A	1: 45,366,807 (GRCm39)		probably null	Het
Crocc2	C	T	1: 93,121,809 (GRCm39)	L653F	probably damaging	Het
Cry2	A	G	2: 92,244,009 (GRCm39)	L388P	probably damaging	Het
Cstb	T	C	10: 78,262,822 (GRCm39)	F43S	probably damaging	Het
Ctcfl	A	G	2: 172,954,051 (GRCm39)	Y349H	probably damaging	Het
Ctu2	T	A	8: 123,208,238 (GRCm39)	V485D	probably damaging	Het
Cwc25	A	C	11: 97,638,823 (GRCm39)	L367R	possibly damaging	Het
Dock4	T	A	12: 40,838,243 (GRCm39)	H1061Q	possibly damaging	Het
Dpy19l3	C	A	7: 35,452,067 (GRCm39)	V26L	probably benign	Het
Efcab3	T	C	11: 104,605,528 (GRCm39)	I298T	probably benign	Het
Elmo1	A	T	13: 20,465,105 (GRCm39)	I225F	probably benign	Het
Ep300	T	C	15: 81,534,746 (GRCm39)	S2268P	probably damaging	Het
F2r	C	T	13: 95,754,836 (GRCm39)	C16Y	probably damaging	Het
Fbxo33	C	A	12: 59,251,157 (GRCm39)	E453*	probably null	Het
Flot1	A	T	17: 36,136,405 (GRCm39)	D186V	probably damaging	Het
Fmn2	CCCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATATCTCCCCTACCTCCTCTCCCTGGAATGGGAATACCTCC	CCCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATATCTCCCCTACCTCCTCTCCCTGGAATGGGAATACCTCC	1: 174,436,769 (GRCm39)		probably benign	Het
Fras1	T	C	5: 96,744,644 (GRCm39)	C560R	probably damaging	Het
Get3	A	T	8: 85,746,492 (GRCm39)	F119I	probably benign	Het
Gfi1	A	T	5: 107,871,095 (GRCm39)	S165T	possibly damaging	Het
Gli2	T	C	1: 118,776,222 (GRCm39)	D400G	probably damaging	Het
Gm37240	T	A	3: 84,879,944 (GRCm39)		probably null	Het
Gml	C	T	15: 74,688,970 (GRCm39)	V27I	possibly damaging	Het
Htt	A	T	5: 34,961,143 (GRCm39)	I257F	probably benign	Het
Hycc2	T	C	1: 58,569,193 (GRCm39)	K462R	probably benign	Het
Ier5	C	T	1: 154,974,438 (GRCm39)	V247M	probably damaging	Het
Ift46	C	T	9: 44,689,892 (GRCm39)		probably benign	Het
Il12b	A	G	11: 44,298,721 (GRCm39)	T59A	probably benign	Het
Insr	A	G	8: 3,248,231 (GRCm39)	L438P	probably damaging	Het
Iqgap2	C	T	13: 95,869,398 (GRCm39)		probably null	Het
Irx4	A	T	13: 73,415,672 (GRCm39)	T154S	possibly damaging	Het
Itgbl1	A	G	14: 124,094,901 (GRCm39)	K309E	probably benign	Het
Kcns2	T	A	15: 34,839,849 (GRCm39)	L404*	probably null	Het
Kif2c	T	C	4: 117,019,226 (GRCm39)	E644G	possibly damaging	Het
Klhl22	T	C	16: 17,594,669 (GRCm39)	L266P	probably damaging	Het
Krt71	T	A	15: 101,646,764 (GRCm39)	K315*	probably null	Het
Lca5	T	C	9: 83,277,583 (GRCm39)	N587S	probably benign	Het
Lrp1b	T	A	2: 41,185,681 (GRCm39)	M1459L		Het
Lrrtm2	T	C	18: 35,346,645 (GRCm39)	K219R	probably damaging	Het
Mcmbp	T	C	7: 128,306,241 (GRCm39)	D459G	probably damaging	Het
Megf8	A	G	7: 25,048,367 (GRCm39)	H1680R	probably damaging	Het
Mia3	A	G	1: 183,115,629 (GRCm39)	S301P	probably benign	Het
Mtrr	A	G	13: 68,712,521 (GRCm39)	Y622H	probably damaging	Het
Myo7b	T	A	18: 32,099,292 (GRCm39)	Q1710L	probably damaging	Het
Nab1	T	C	1: 52,520,154 (GRCm39)	N276D	probably damaging	Het
Nalcn	C	T	14: 123,540,302 (GRCm39)	R1127H	probably damaging	Het
Nbas	T	A	12: 13,324,220 (GRCm39)	I110N	probably damaging	Het
Nfe2l3	C	T	6: 51,434,276 (GRCm39)	Q279*	probably null	Het
Nlrp9c	C	T	7: 26,064,440 (GRCm39)	E963K	probably benign	Het
Nsmaf	A	G	4: 6,416,586 (GRCm39)	V569A	probably benign	Het
Or10ak14	T	C	4: 118,611,372 (GRCm39)	Y123C	possibly damaging	Het
Or13c3	C	T	4: 52,856,421 (GRCm39)	V31I	probably benign	Het
Or52a5b	T	A	7: 103,417,137 (GRCm39)	I156F	probably benign	Het
Or52n5	A	T	7: 104,588,638 (GRCm39)	I302F	probably benign	Het
Or5ac17	T	A	16: 59,036,283 (GRCm39)	D231V	probably benign	Het
Or5p4	T	C	7: 107,680,399 (GRCm39)	S133P	not run	Het
Or7e166	A	T	9: 19,624,183 (GRCm39)	D20V	probably damaging	Het
Or7g32	T	A	9: 19,408,398 (GRCm39)	M118K	probably damaging	Het
Or8d1b	T	A	9: 38,887,017 (GRCm39)	V15E	probably benign	Het
Or8k28	T	C	2: 86,286,412 (GRCm39)	T68A	probably benign	Het
Paf1	A	G	7: 28,096,353 (GRCm39)	Y322C	probably damaging	Het
Pcdha2	C	A	18: 37,072,438 (GRCm39)	A23D	possibly damaging	Het
Pcdhb6	G	A	18: 37,468,225 (GRCm39)	C382Y	probably damaging	Het
Pdzd7	C	T	19: 45,034,045 (GRCm39)	G13D	probably damaging	Het
Pik3r2	T	C	8: 71,222,025 (GRCm39)	N586S	probably benign	Het
Pik3r3	T	A	4: 116,128,027 (GRCm39)	D119E	probably benign	Het
Pkd2l1	T	A	19: 44,142,154 (GRCm39)	I535F	probably benign	Het
Ppfia3	T	C	7: 45,010,870 (GRCm39)		probably null	Het
Ppl	T	A	16: 4,930,860 (GRCm39)	Q53L	possibly damaging	Het
Rimbp3	T	G	16: 17,029,068 (GRCm39)	S831A	probably benign	Het
Rnps1	A	G	17: 24,637,504 (GRCm39)	T49A	unknown	Het
Rpl7a	T	A	2: 26,802,019 (GRCm39)		probably null	Het
Rrm2b	T	A	15: 37,931,891 (GRCm39)	E248D	probably benign	Het
Sdhaf4	A	G	1: 24,035,439 (GRCm39)	F70L	probably damaging	Het
Sel1l3	A	T	5: 53,273,751 (GRCm39)	S1038R	probably benign	Het
Selenoo	T	A	15: 88,973,681 (GRCm39)	F124I	probably benign	Het
Setbp1	A	T	18: 78,900,701 (GRCm39)	Y989N	probably damaging	Het
Slc1a7	A	G	4: 107,859,400 (GRCm39)	E163G	possibly damaging	Het
Slc22a2	T	C	17: 12,831,278 (GRCm39)	F356S	probably damaging	Het
Slc24a4	C	T	12: 102,205,435 (GRCm39)	P378L	probably benign	Het
Slc4a1ap	A	G	5: 31,684,871 (GRCm39)	D169G	probably benign	Het
Slc4a7	T	A	14: 14,757,421 (GRCm38)	V422D	probably damaging	Het
Slitrk6	T	C	14: 110,987,295 (GRCm39)	Y804C	probably damaging	Het
Smarcad1	T	A	6: 65,087,360 (GRCm39)	H906Q	probably benign	Het
Smn1	A	G	13: 100,264,373 (GRCm39)	T109A	probably damaging	Het
Sntb2	A	G	8: 107,707,944 (GRCm39)	N236S	probably damaging	Het
Spag9	T	A	11: 94,005,177 (GRCm39)		probably null	Het
Spg11	C	A	2: 121,888,910 (GRCm39)	G2154W	probably damaging	Het
Srsf6	T	C	2: 162,776,489 (GRCm39)	S278P	unknown	Het
Stag3	G	A	5: 138,280,222 (GRCm39)	R29H	probably benign	Het
Stambp	C	T	6: 83,540,888 (GRCm39)	V98I	not run	Het
Styxl2	G	T	1: 165,939,632 (GRCm39)	S58*	probably null	Het
Suco	T	C	1: 161,689,780 (GRCm39)	D89G	possibly damaging	Het
Suox	C	T	10: 128,506,910 (GRCm39)	V373I	probably benign	Het
Sv2b	T	A	7: 74,797,476 (GRCm39)		probably null	Het
Tatdn2	T	C	6: 113,681,662 (GRCm39)	L565P	probably damaging	Het
Tbpl2	T	A	2: 23,984,712 (GRCm39)	E145V	probably benign	Het
Tlr1	T	C	5: 65,082,571 (GRCm39)	I669V	not run	Het
Tmc1	A	T	19: 20,845,753 (GRCm39)	F157I	probably damaging	Het
Tmem26	T	G	10: 68,559,922 (GRCm39)		probably null	Het
Tmem33	A	T	5: 67,443,476 (GRCm39)	I218F	probably benign	Het
Tmem94	T	A	11: 115,685,000 (GRCm39)	D862E	probably damaging	Het
Tnr	T	C	1: 159,712,432 (GRCm39)		probably null	Het
Tnrc6c	T	C	11: 117,632,606 (GRCm39)	S1103P	probably benign	Het
Tomm70a	T	A	16: 56,966,407 (GRCm39)	Y453*	probably null	Het
Trabd	C	A	15: 88,969,493 (GRCm39)	R229S	possibly damaging	Het
Trappc10	T	C	10: 78,029,252 (GRCm39)	D1095G	probably damaging	Het
Trim27	A	G	13: 21,376,631 (GRCm39)	T460A	possibly damaging	Het
Tsen54	T	G	11: 115,712,531 (GRCm39)	D433E	probably benign	Het
Ubap2	A	G	4: 41,235,515 (GRCm39)		probably null	Het
Vat1l	A	T	8: 115,016,132 (GRCm39)	N341Y	possibly damaging	Het
Vmn2r82	C	G	10: 79,232,276 (GRCm39)	Y758*	probably null	Het
Vps16	T	G	2: 130,280,099 (GRCm39)	V108G	probably damaging	Het
Vps53	T	A	11: 75,967,900 (GRCm39)	I585F	possibly damaging	Het
Wrnip1	G	A	13: 33,000,264 (GRCm39)	V424M	probably benign	Het
Yif1a	G	A	19: 5,139,818 (GRCm39)	V88M	possibly damaging	Het
Zfat	T	C	15: 68,052,969 (GRCm39)	Y275C	probably damaging	Het
Zfhx3	A	G	8: 109,519,880 (GRCm39)	E334G	probably damaging	Het
Zfp277	T	C	12: 40,365,852 (GRCm39)	D539G	possibly damaging	Het
Zfp292	A	T	4: 34,808,384 (GRCm39)	N1558K	probably benign	Het
Zfp3	A	G	11: 70,662,899 (GRCm39)	K286R	probably benign	Het
Zfp366	T	C	13: 99,366,023 (GRCm39)	C395R	probably damaging	Het

Other mutations in Asxl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01409:Asxl1	APN	2	153,234,860 (GRCm39)	splice site	probably benign
IGL01432:Asxl1	APN	2	153,242,125 (GRCm39)	missense	probably benign	0.38
IGL01543:Asxl1	APN	2	153,243,404 (GRCm39)	missense	probably benign	0.11
IGL02355:Asxl1	APN	2	153,243,706 (GRCm39)	missense	probably benign	0.34
IGL02362:Asxl1	APN	2	153,243,706 (GRCm39)	missense	probably benign	0.34
IGL02645:Asxl1	APN	2	153,234,777 (GRCm39)	missense	possibly damaging	0.94
IGL02696:Asxl1	APN	2	153,242,115 (GRCm39)	nonsense	probably null
IGL03365:Asxl1	APN	2	153,243,674 (GRCm39)	missense	probably damaging	1.00
IGL03372:Asxl1	APN	2	153,242,333 (GRCm39)	missense	probably damaging	0.99
IGL03377:Asxl1	APN	2	153,238,700 (GRCm39)	missense	probably damaging	1.00
astrophel	UTSW	2	153,242,026 (GRCm39)	missense	possibly damaging	0.75
hairbrush	UTSW	2	153,242,644 (GRCm39)	missense	possibly damaging	0.55
R0044:Asxl1	UTSW	2	153,242,129 (GRCm39)	missense	probably benign	0.06
R0044:Asxl1	UTSW	2	153,242,129 (GRCm39)	missense	probably benign	0.06
R0600:Asxl1	UTSW	2	153,241,824 (GRCm39)	missense	probably benign	0.00
R0659:Asxl1	UTSW	2	153,242,644 (GRCm39)	missense	possibly damaging	0.55
R0661:Asxl1	UTSW	2	153,242,644 (GRCm39)	missense	possibly damaging	0.55
R0684:Asxl1	UTSW	2	153,239,442 (GRCm39)	missense	probably damaging	1.00
R1606:Asxl1	UTSW	2	153,242,375 (GRCm39)	missense	probably damaging	0.99
R1747:Asxl1	UTSW	2	153,235,374 (GRCm39)	missense	possibly damaging	0.86
R1796:Asxl1	UTSW	2	153,243,526 (GRCm39)	missense	probably benign	0.31
R1914:Asxl1	UTSW	2	153,243,826 (GRCm39)	missense	probably damaging	1.00
R2099:Asxl1	UTSW	2	153,194,187 (GRCm39)	missense	possibly damaging	0.95
R2373:Asxl1	UTSW	2	153,243,820 (GRCm39)	missense	probably benign	0.13
R2910:Asxl1	UTSW	2	153,242,959 (GRCm39)	missense	probably benign	0.00
R3620:Asxl1	UTSW	2	153,199,075 (GRCm39)	missense	probably damaging	1.00
R3701:Asxl1	UTSW	2	153,241,264 (GRCm39)	missense	probably benign	0.04
R4200:Asxl1	UTSW	2	153,242,026 (GRCm39)	missense	possibly damaging	0.75
R4773:Asxl1	UTSW	2	153,243,905 (GRCm39)	missense	probably damaging	1.00
R4902:Asxl1	UTSW	2	153,241,751 (GRCm39)	missense	probably benign	0.02
R5100:Asxl1	UTSW	2	153,239,851 (GRCm39)	missense	probably damaging	1.00
R5102:Asxl1	UTSW	2	153,242,875 (GRCm39)	missense	probably benign	0.00
R5166:Asxl1	UTSW	2	153,243,041 (GRCm39)	missense	probably damaging	1.00
R5421:Asxl1	UTSW	2	153,241,504 (GRCm39)	missense	probably benign	0.04
R5701:Asxl1	UTSW	2	153,241,409 (GRCm39)	missense	probably damaging	1.00
R5861:Asxl1	UTSW	2	153,241,310 (GRCm39)	missense	probably damaging	0.99
R5973:Asxl1	UTSW	2	153,243,931 (GRCm39)	missense	probably damaging	0.97
R6384:Asxl1	UTSW	2	153,233,744 (GRCm39)	critical splice donor site	probably null
R7023:Asxl1	UTSW	2	153,242,469 (GRCm39)	missense	probably benign	0.00
R7028:Asxl1	UTSW	2	153,242,027 (GRCm39)	missense	probably benign	0.00
R7176:Asxl1	UTSW	2	153,243,908 (GRCm39)	missense	probably damaging	1.00
R7297:Asxl1	UTSW	2	153,239,355 (GRCm39)	missense	probably benign	0.01
R7464:Asxl1	UTSW	2	153,239,705 (GRCm39)	missense	probably benign	0.01
R7678:Asxl1	UTSW	2	153,242,572 (GRCm39)	missense	probably damaging	1.00
R7686:Asxl1	UTSW	2	153,233,534 (GRCm39)	missense	probably damaging	1.00
R7789:Asxl1	UTSW	2	153,241,943 (GRCm39)	missense	probably benign	0.00
R7838:Asxl1	UTSW	2	153,238,733 (GRCm39)	missense	probably damaging	1.00
R7898:Asxl1	UTSW	2	153,241,854 (GRCm39)	missense	possibly damaging	0.65
R8281:Asxl1	UTSW	2	153,241,321 (GRCm39)	missense	probably damaging	1.00
R8354:Asxl1	UTSW	2	153,235,345 (GRCm39)	missense	probably benign	0.40
R8383:Asxl1	UTSW	2	153,235,639 (GRCm39)	missense	probably damaging	1.00
R8995:Asxl1	UTSW	2	153,235,886 (GRCm39)	missense	probably damaging	1.00
R9183:Asxl1	UTSW	2	153,239,840 (GRCm39)	missense	probably damaging	0.99
X0024:Asxl1	UTSW	2	153,243,905 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCTCTGCTACCTTTCCCAAAG -3'
(R):5'- AGCACTATAAATCTCATGGGGAG -3'

Sequencing Primer
(F):5'- TTCCCAAAGGCCTTGCTGG -3'
(R):5'- CTCATGGGGAGGCAAAATTTATC -3'

Posted On

2019-09-13