Mutagenetix > Incidental Mutation

Incidental Mutation 'R7378:Zfhx3'

572484

Institutional Source

Beutler Lab

Gene Symbol

Zfhx3

Ensembl Gene

ENSMUSG00000038872

Gene Name

zinc finger homeobox 3

Synonyms

Sci, A230102L03Rik, WBP9, Atbf1

MMRRC Submission

045460-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.919) question?

Stock #

R7378 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

109005975-109688268 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 109519880 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 334 (E334G)

Ref Sequence

ENSEMBL: ENSMUSP00000044612 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000043896] [ENSMUST00000188994] [ENSMUST00000220518]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000043896
AA Change: E334G

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000044612
Gene: ENSMUSG00000038872
AA Change: E334G

Domain	Start	End	E-Value	Type
ZnF_C2H2	79	103	7.89e0	SMART
low complexity region	110	127	N/A	INTRINSIC
low complexity region	148	165	N/A	INTRINSIC
ZnF_C2H2	282	305	1.36e1	SMART
low complexity region	393	411	N/A	INTRINSIC
coiled coil region	453	496	N/A	INTRINSIC
low complexity region	500	523	N/A	INTRINSIC
ZnF_C2H2	641	664	3.47e0	SMART
ZnF_C2H2	672	695	6.78e-3	SMART
ZnF_U1	724	758	5.71e-1	SMART
ZnF_C2H2	727	751	4.87e-4	SMART
low complexity region	771	785	N/A	INTRINSIC
low complexity region	796	804	N/A	INTRINSIC
ZnF_C2H2	805	829	6.67e-2	SMART
ZnF_U1	982	1016	2.35e0	SMART
ZnF_C2H2	985	1009	4.57e0	SMART
ZnF_C2H2	1041	1065	3.99e0	SMART
ZnF_U1	1086	1120	1.36e0	SMART
ZnF_C2H2	1089	1113	1.33e-1	SMART
ZnF_C2H2	1233	1256	4.11e-2	SMART
ZnF_C2H2	1262	1285	4.34e-1	SMART
ZnF_C2H2	1370	1395	1.08e-1	SMART
ZnF_C2H2	1411	1433	3.34e-2	SMART
ZnF_C2H2	1439	1462	8.09e-1	SMART
low complexity region	1500	1512	N/A	INTRINSIC
ZnF_U1	1552	1586	1.05e0	SMART
ZnF_C2H2	1555	1579	8.22e-2	SMART
ZnF_U1	1603	1637	4.19e0	SMART
ZnF_C2H2	1606	1630	1.16e-1	SMART
low complexity region	1643	1669	N/A	INTRINSIC
low complexity region	1734	1776	N/A	INTRINSIC
low complexity region	1792	1802	N/A	INTRINSIC
low complexity region	1842	1878	N/A	INTRINSIC
low complexity region	1881	1894	N/A	INTRINSIC
low complexity region	1967	1985	N/A	INTRINSIC
ZnF_C2H2	1990	2013	1.62e0	SMART
low complexity region	2041	2088	N/A	INTRINSIC
low complexity region	2110	2125	N/A	INTRINSIC
HOX	2152	2214	1.13e-16	SMART
HOX	2249	2311	2.41e-20	SMART
ZnF_C2H2	2335	2355	1.72e1	SMART
low complexity region	2383	2414	N/A	INTRINSIC
low complexity region	2458	2473	N/A	INTRINSIC
low complexity region	2476	2521	N/A	INTRINSIC
ZnF_C2H2	2539	2561	1.79e-2	SMART
low complexity region	2606	2619	N/A	INTRINSIC
HOX	2650	2712	2.97e-20	SMART
ZnF_C2H2	2720	2743	7.67e-2	SMART
low complexity region	2929	2950	N/A	INTRINSIC
HOX	2954	3016	1.07e-17	SMART
ZnF_U1	3029	3063	1.8e-1	SMART
ZnF_C2H2	3032	3056	8.31e0	SMART
low complexity region	3130	3144	N/A	INTRINSIC
low complexity region	3181	3235	N/A	INTRINSIC
low complexity region	3237	3256	N/A	INTRINSIC
low complexity region	3268	3282	N/A	INTRINSIC
low complexity region	3290	3299	N/A	INTRINSIC
coiled coil region	3362	3417	N/A	INTRINSIC
low complexity region	3452	3476	N/A	INTRINSIC
ZnF_C2H2	3489	3509	1.45e2	SMART
ZnF_U1	3546	3580	1.36e0	SMART
ZnF_C2H2	3549	3573	1.77e1	SMART
low complexity region	3602	3633	N/A	INTRINSIC
low complexity region	3642	3674	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000188994

Predicted Effect

probably damaging
Transcript: ENSMUST00000220518
AA Change: E334G

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

99% (138/140)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a transcription factor with multiple homeodomains and zinc finger motifs, and regulates myogenic and neuronal differentiation. The encoded protein suppresses expression of the alpha-fetoprotein gene by binding to an AT-rich enhancer motif. The protein has also been shown to negatively regulate c-Myb, and transactivate the cell cycle inhibitor cyclin-dependent kinase inhibitor 1A (also known as p21CIP1). This gene is reported to function as a tumor suppressor in several cancers, and sequence variants of this gene are also associated with atrial fibrillation. Multiple transcript variants expressed from alternate promoters and encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
PHENOTYPE: Mice homozygous for a gene trapped allele exhibit normal initial pituitary development but reduced GH and TSH-beta staining within the pituitary by E17.5. Mice homozygous for a knock-out allele exhibit prenatal lethality. Mice heterozygous for the same allele exhibit partial postnatal lethality, decreased body size and prolonged conception time. [provided by MGI curators]

Allele List at MGI

All alleles(18) : Gene trapped(18)

Other mutations in this stock

Total: 142 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2ml1	A	C	6: 128,523,210 (GRCm39)		probably null	Het
Aars1	A	G	8: 111,768,974 (GRCm39)	Y258C	probably damaging	Het
Acaa2	A	T	18: 74,938,943 (GRCm39)	Y376F	probably benign	Het
Adcy1	C	T	11: 7,119,543 (GRCm39)	S1062F	possibly damaging	Het
Adgrb3	A	T	1: 25,571,000 (GRCm39)	C492*	probably null	Het
Adgrg6	A	G	10: 14,411,636 (GRCm39)	F22S	probably benign	Het
Adh1	A	G	3: 137,994,648 (GRCm39)		probably null	Het
Afm	T	A	5: 90,699,259 (GRCm39)	C568S	probably benign	Het
Ago4	C	T	4: 126,405,257 (GRCm39)	E439K	probably benign	Het
Aifm2	T	G	10: 61,563,496 (GRCm39)	V122G	possibly damaging	Het
Akap6	A	G	12: 53,189,357 (GRCm39)	N2257S	probably benign	Het
Ap3m2	A	G	8: 23,294,026 (GRCm39)	I8T	probably benign	Het
Apc2	C	A	10: 80,147,228 (GRCm39)	L761I	probably damaging	Het
Asap2	A	G	12: 21,162,052 (GRCm39)	D3G	probably benign	Het
Asxl1	T	C	2: 153,243,913 (GRCm39)	C1489R	probably damaging	Het
Atm	T	G	9: 53,364,737 (GRCm39)		probably null	Het
Bmal1	C	A	7: 112,898,415 (GRCm39)	T281K	probably benign	Het
Bpifa6	C	A	2: 153,828,353 (GRCm39)	A153E	probably damaging	Het
Cav2	A	G	6: 17,282,059 (GRCm39)	T106A	probably benign	Het
Cavin4	A	G	4: 48,663,631 (GRCm39)	N4D	probably benign	Het
Cenpc1	T	C	5: 86,194,358 (GRCm39)	N136S	probably benign	Het
Cfap45	T	A	1: 172,365,910 (GRCm39)		probably null	Het
Col3a1	G	A	1: 45,366,807 (GRCm39)		probably null	Het
Crocc2	C	T	1: 93,121,809 (GRCm39)	L653F	probably damaging	Het
Cry2	A	G	2: 92,244,009 (GRCm39)	L388P	probably damaging	Het
Cstb	T	C	10: 78,262,822 (GRCm39)	F43S	probably damaging	Het
Ctcfl	A	G	2: 172,954,051 (GRCm39)	Y349H	probably damaging	Het
Ctu2	T	A	8: 123,208,238 (GRCm39)	V485D	probably damaging	Het
Cwc25	A	C	11: 97,638,823 (GRCm39)	L367R	possibly damaging	Het
Dock4	T	A	12: 40,838,243 (GRCm39)	H1061Q	possibly damaging	Het
Dpy19l3	C	A	7: 35,452,067 (GRCm39)	V26L	probably benign	Het
Efcab3	T	C	11: 104,605,528 (GRCm39)	I298T	probably benign	Het
Elmo1	A	T	13: 20,465,105 (GRCm39)	I225F	probably benign	Het
Ep300	T	C	15: 81,534,746 (GRCm39)	S2268P	probably damaging	Het
F2r	C	T	13: 95,754,836 (GRCm39)	C16Y	probably damaging	Het
Fbxo33	C	A	12: 59,251,157 (GRCm39)	E453*	probably null	Het
Flot1	A	T	17: 36,136,405 (GRCm39)	D186V	probably damaging	Het
Fmn2	CCCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATATCTCCCCTACCTCCTCTCCCTGGAATGGGAATACCTCC	CCCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATATCTCCCCTACCTCCTCTCCCTGGAATGGGAATACCTCC	1: 174,436,769 (GRCm39)		probably benign	Het
Fras1	T	C	5: 96,744,644 (GRCm39)	C560R	probably damaging	Het
Get3	A	T	8: 85,746,492 (GRCm39)	F119I	probably benign	Het
Gfi1	A	T	5: 107,871,095 (GRCm39)	S165T	possibly damaging	Het
Gli2	T	C	1: 118,776,222 (GRCm39)	D400G	probably damaging	Het
Gm37240	T	A	3: 84,879,944 (GRCm39)		probably null	Het
Gml	C	T	15: 74,688,970 (GRCm39)	V27I	possibly damaging	Het
Htt	A	T	5: 34,961,143 (GRCm39)	I257F	probably benign	Het
Hycc2	T	C	1: 58,569,193 (GRCm39)	K462R	probably benign	Het
Ier5	C	T	1: 154,974,438 (GRCm39)	V247M	probably damaging	Het
Ift46	C	T	9: 44,689,892 (GRCm39)		probably benign	Het
Il12b	A	G	11: 44,298,721 (GRCm39)	T59A	probably benign	Het
Insr	A	G	8: 3,248,231 (GRCm39)	L438P	probably damaging	Het
Iqgap2	C	T	13: 95,869,398 (GRCm39)		probably null	Het
Irx4	A	T	13: 73,415,672 (GRCm39)	T154S	possibly damaging	Het
Itgbl1	A	G	14: 124,094,901 (GRCm39)	K309E	probably benign	Het
Kcns2	T	A	15: 34,839,849 (GRCm39)	L404*	probably null	Het
Kif2c	T	C	4: 117,019,226 (GRCm39)	E644G	possibly damaging	Het
Klhl22	T	C	16: 17,594,669 (GRCm39)	L266P	probably damaging	Het
Krt71	T	A	15: 101,646,764 (GRCm39)	K315*	probably null	Het
Lca5	T	C	9: 83,277,583 (GRCm39)	N587S	probably benign	Het
Lrp1b	T	A	2: 41,185,681 (GRCm39)	M1459L		Het
Lrrtm2	T	C	18: 35,346,645 (GRCm39)	K219R	probably damaging	Het
Mcmbp	T	C	7: 128,306,241 (GRCm39)	D459G	probably damaging	Het
Megf8	A	G	7: 25,048,367 (GRCm39)	H1680R	probably damaging	Het
Mia3	A	G	1: 183,115,629 (GRCm39)	S301P	probably benign	Het
Mtrr	A	G	13: 68,712,521 (GRCm39)	Y622H	probably damaging	Het
Myo7b	T	A	18: 32,099,292 (GRCm39)	Q1710L	probably damaging	Het
Nab1	T	C	1: 52,520,154 (GRCm39)	N276D	probably damaging	Het
Nalcn	C	T	14: 123,540,302 (GRCm39)	R1127H	probably damaging	Het
Nbas	T	A	12: 13,324,220 (GRCm39)	I110N	probably damaging	Het
Nfe2l3	C	T	6: 51,434,276 (GRCm39)	Q279*	probably null	Het
Nlrp9c	C	T	7: 26,064,440 (GRCm39)	E963K	probably benign	Het
Nsmaf	A	G	4: 6,416,586 (GRCm39)	V569A	probably benign	Het
Or10ak14	T	C	4: 118,611,372 (GRCm39)	Y123C	possibly damaging	Het
Or13c3	C	T	4: 52,856,421 (GRCm39)	V31I	probably benign	Het
Or52a5b	T	A	7: 103,417,137 (GRCm39)	I156F	probably benign	Het
Or52n5	A	T	7: 104,588,638 (GRCm39)	I302F	probably benign	Het
Or5ac17	T	A	16: 59,036,283 (GRCm39)	D231V	probably benign	Het
Or5p4	T	C	7: 107,680,399 (GRCm39)	S133P	not run	Het
Or7e166	A	T	9: 19,624,183 (GRCm39)	D20V	probably damaging	Het
Or7g32	T	A	9: 19,408,398 (GRCm39)	M118K	probably damaging	Het
Or8d1b	T	A	9: 38,887,017 (GRCm39)	V15E	probably benign	Het
Or8k28	T	C	2: 86,286,412 (GRCm39)	T68A	probably benign	Het
Paf1	A	G	7: 28,096,353 (GRCm39)	Y322C	probably damaging	Het
Pcdha2	C	A	18: 37,072,438 (GRCm39)	A23D	possibly damaging	Het
Pcdhb6	G	A	18: 37,468,225 (GRCm39)	C382Y	probably damaging	Het
Pdzd7	C	T	19: 45,034,045 (GRCm39)	G13D	probably damaging	Het
Pik3r2	T	C	8: 71,222,025 (GRCm39)	N586S	probably benign	Het
Pik3r3	T	A	4: 116,128,027 (GRCm39)	D119E	probably benign	Het
Pkd2l1	T	A	19: 44,142,154 (GRCm39)	I535F	probably benign	Het
Ppfia3	T	C	7: 45,010,870 (GRCm39)		probably null	Het
Ppl	T	A	16: 4,930,860 (GRCm39)	Q53L	possibly damaging	Het
Rimbp3	T	G	16: 17,029,068 (GRCm39)	S831A	probably benign	Het
Rnps1	A	G	17: 24,637,504 (GRCm39)	T49A	unknown	Het
Rpl7a	T	A	2: 26,802,019 (GRCm39)		probably null	Het
Rrm2b	T	A	15: 37,931,891 (GRCm39)	E248D	probably benign	Het
Sdhaf4	A	G	1: 24,035,439 (GRCm39)	F70L	probably damaging	Het
Sel1l3	A	T	5: 53,273,751 (GRCm39)	S1038R	probably benign	Het
Selenoo	T	A	15: 88,973,681 (GRCm39)	F124I	probably benign	Het
Setbp1	A	T	18: 78,900,701 (GRCm39)	Y989N	probably damaging	Het
Slc1a7	A	G	4: 107,859,400 (GRCm39)	E163G	possibly damaging	Het
Slc22a2	T	C	17: 12,831,278 (GRCm39)	F356S	probably damaging	Het
Slc24a4	C	T	12: 102,205,435 (GRCm39)	P378L	probably benign	Het
Slc4a1ap	A	G	5: 31,684,871 (GRCm39)	D169G	probably benign	Het
Slc4a7	T	A	14: 14,757,421 (GRCm38)	V422D	probably damaging	Het
Slitrk6	T	C	14: 110,987,295 (GRCm39)	Y804C	probably damaging	Het
Smarcad1	T	A	6: 65,087,360 (GRCm39)	H906Q	probably benign	Het
Smn1	A	G	13: 100,264,373 (GRCm39)	T109A	probably damaging	Het
Sntb2	A	G	8: 107,707,944 (GRCm39)	N236S	probably damaging	Het
Spag9	T	A	11: 94,005,177 (GRCm39)		probably null	Het
Spg11	C	A	2: 121,888,910 (GRCm39)	G2154W	probably damaging	Het
Srsf6	T	C	2: 162,776,489 (GRCm39)	S278P	unknown	Het
Stag3	G	A	5: 138,280,222 (GRCm39)	R29H	probably benign	Het
Stambp	C	T	6: 83,540,888 (GRCm39)	V98I	not run	Het
Styxl2	G	T	1: 165,939,632 (GRCm39)	S58*	probably null	Het
Suco	T	C	1: 161,689,780 (GRCm39)	D89G	possibly damaging	Het
Suox	C	T	10: 128,506,910 (GRCm39)	V373I	probably benign	Het
Sv2b	T	A	7: 74,797,476 (GRCm39)		probably null	Het
Tatdn2	T	C	6: 113,681,662 (GRCm39)	L565P	probably damaging	Het
Tbpl2	T	A	2: 23,984,712 (GRCm39)	E145V	probably benign	Het
Tlr1	T	C	5: 65,082,571 (GRCm39)	I669V	not run	Het
Tmc1	A	T	19: 20,845,753 (GRCm39)	F157I	probably damaging	Het
Tmem26	T	G	10: 68,559,922 (GRCm39)		probably null	Het
Tmem33	A	T	5: 67,443,476 (GRCm39)	I218F	probably benign	Het
Tmem94	T	A	11: 115,685,000 (GRCm39)	D862E	probably damaging	Het
Tnr	T	C	1: 159,712,432 (GRCm39)		probably null	Het
Tnrc6c	T	C	11: 117,632,606 (GRCm39)	S1103P	probably benign	Het
Tomm70a	T	A	16: 56,966,407 (GRCm39)	Y453*	probably null	Het
Trabd	C	A	15: 88,969,493 (GRCm39)	R229S	possibly damaging	Het
Trappc10	T	C	10: 78,029,252 (GRCm39)	D1095G	probably damaging	Het
Trim27	A	G	13: 21,376,631 (GRCm39)	T460A	possibly damaging	Het
Tsen54	T	G	11: 115,712,531 (GRCm39)	D433E	probably benign	Het
Ubap2	A	G	4: 41,235,515 (GRCm39)		probably null	Het
Vat1l	A	T	8: 115,016,132 (GRCm39)	N341Y	possibly damaging	Het
Vmn2r82	C	G	10: 79,232,276 (GRCm39)	Y758*	probably null	Het
Vps16	T	G	2: 130,280,099 (GRCm39)	V108G	probably damaging	Het
Vps53	T	A	11: 75,967,900 (GRCm39)	I585F	possibly damaging	Het
Wrnip1	G	A	13: 33,000,264 (GRCm39)	V424M	probably benign	Het
Yif1a	G	A	19: 5,139,818 (GRCm39)	V88M	possibly damaging	Het
Zfat	T	C	15: 68,052,969 (GRCm39)	Y275C	probably damaging	Het
Zfp277	T	C	12: 40,365,852 (GRCm39)	D539G	possibly damaging	Het
Zfp292	A	T	4: 34,808,384 (GRCm39)	N1558K	probably benign	Het
Zfp3	A	G	11: 70,662,899 (GRCm39)	K286R	probably benign	Het
Zfp366	T	C	13: 99,366,023 (GRCm39)	C395R	probably damaging	Het

Other mutations in Zfhx3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01011:Zfhx3	APN	8	109,520,226 (GRCm39)	missense	probably benign	0.00
IGL01946:Zfhx3	APN	8	109,660,561 (GRCm39)	missense	probably damaging	0.98
IGL01973:Zfhx3	APN	8	109,673,825 (GRCm39)	missense	probably damaging	1.00
IGL01983:Zfhx3	APN	8	109,673,866 (GRCm39)	missense	probably damaging	1.00
IGL02151:Zfhx3	APN	8	109,520,515 (GRCm39)	missense	probably damaging	1.00
IGL02405:Zfhx3	APN	8	109,682,374 (GRCm39)	missense	unknown
IGL02406:Zfhx3	APN	8	109,682,374 (GRCm39)	missense	unknown
IGL02408:Zfhx3	APN	8	109,682,004 (GRCm39)	splice site	probably benign
IGL02549:Zfhx3	APN	8	109,527,141 (GRCm39)	missense	probably damaging	1.00
IGL02601:Zfhx3	APN	8	109,583,462 (GRCm39)	missense	probably damaging	1.00
IGL02649:Zfhx3	APN	8	109,520,167 (GRCm39)	missense	possibly damaging	0.94
IGL03027:Zfhx3	APN	8	109,519,820 (GRCm39)	missense	probably damaging	0.98
IGL03053:Zfhx3	APN	8	109,673,132 (GRCm39)	missense	probably damaging	0.99
IGL03168:Zfhx3	APN	8	109,673,132 (GRCm39)	missense	probably damaging	0.99
IGL03194:Zfhx3	APN	8	109,521,359 (GRCm39)	missense	probably damaging	0.97
IGL03248:Zfhx3	APN	8	109,673,182 (GRCm39)	missense	probably damaging	1.00
FR4449:Zfhx3	UTSW	8	109,682,726 (GRCm39)	small insertion	probably benign
FR4589:Zfhx3	UTSW	8	109,682,733 (GRCm39)	small insertion	probably benign
FR4737:Zfhx3	UTSW	8	109,682,735 (GRCm39)	small insertion	probably benign
FR4737:Zfhx3	UTSW	8	109,682,734 (GRCm39)	small insertion	probably benign
FR4737:Zfhx3	UTSW	8	109,682,720 (GRCm39)	small insertion	probably benign
G5030:Zfhx3	UTSW	8	109,678,091 (GRCm39)	missense	possibly damaging	0.86
R0016:Zfhx3	UTSW	8	109,676,810 (GRCm39)	missense	probably benign	0.02
R0090:Zfhx3	UTSW	8	109,676,689 (GRCm39)	missense	possibly damaging	0.85
R0330:Zfhx3	UTSW	8	109,675,589 (GRCm39)	missense	probably damaging	1.00
R0332:Zfhx3	UTSW	8	109,673,255 (GRCm39)	missense	probably damaging	1.00
R0398:Zfhx3	UTSW	8	109,677,878 (GRCm39)	missense	probably damaging	0.98
R0539:Zfhx3	UTSW	8	109,527,141 (GRCm39)	missense	probably damaging	1.00
R0546:Zfhx3	UTSW	8	109,520,819 (GRCm39)	missense	probably damaging	1.00
R0614:Zfhx3	UTSW	8	109,675,599 (GRCm39)	nonsense	probably null
R0614:Zfhx3	UTSW	8	109,675,171 (GRCm39)	missense	probably benign	0.03
R0653:Zfhx3	UTSW	8	109,673,440 (GRCm39)	missense	possibly damaging	0.95
R0718:Zfhx3	UTSW	8	109,682,282 (GRCm39)	missense	unknown
R0825:Zfhx3	UTSW	8	109,675,840 (GRCm39)	missense	probably damaging	0.99
R1143:Zfhx3	UTSW	8	109,521,043 (GRCm39)	missense	probably damaging	1.00
R1319:Zfhx3	UTSW	8	109,660,465 (GRCm39)	missense	probably damaging	0.99
R1347:Zfhx3	UTSW	8	109,527,330 (GRCm39)	splice site	probably benign
R1412:Zfhx3	UTSW	8	109,641,199 (GRCm39)	missense	possibly damaging	0.88
R1447:Zfhx3	UTSW	8	109,675,076 (GRCm39)	missense	probably benign	0.03
R1530:Zfhx3	UTSW	8	109,675,121 (GRCm39)	missense	probably damaging	1.00
R1745:Zfhx3	UTSW	8	109,682,494 (GRCm39)	missense	unknown
R1764:Zfhx3	UTSW	8	109,678,276 (GRCm39)	missense	probably benign	0.18
R1781:Zfhx3	UTSW	8	109,520,167 (GRCm39)	missense	probably benign	0.01
R1917:Zfhx3	UTSW	8	109,682,880 (GRCm39)	missense	unknown
R1956:Zfhx3	UTSW	8	109,520,774 (GRCm39)	missense	probably benign	0.02
R2049:Zfhx3	UTSW	8	109,671,809 (GRCm39)	missense	probably benign	0.01
R2196:Zfhx3	UTSW	8	109,526,885 (GRCm39)	missense	probably damaging	1.00
R3085:Zfhx3	UTSW	8	109,682,664 (GRCm39)	missense	unknown
R3765:Zfhx3	UTSW	8	109,519,394 (GRCm39)	missense	probably damaging	0.97
R4162:Zfhx3	UTSW	8	109,683,619 (GRCm39)	missense	unknown
R4243:Zfhx3	UTSW	8	109,518,952 (GRCm39)	missense	probably damaging	0.97
R4380:Zfhx3	UTSW	8	109,683,022 (GRCm39)	missense	unknown
R4433:Zfhx3	UTSW	8	109,682,269 (GRCm39)	missense	unknown
R4509:Zfhx3	UTSW	8	109,520,411 (GRCm39)	missense	probably benign	0.01
R4731:Zfhx3	UTSW	8	109,682,716 (GRCm39)	missense	unknown
R4788:Zfhx3	UTSW	8	109,520,842 (GRCm39)	missense	probably damaging	1.00
R4812:Zfhx3	UTSW	8	109,674,593 (GRCm39)	missense	possibly damaging	0.83
R4893:Zfhx3	UTSW	8	109,683,639 (GRCm39)	missense	unknown
R4907:Zfhx3	UTSW	8	109,519,986 (GRCm39)	missense	probably damaging	0.99
R4935:Zfhx3	UTSW	8	109,674,482 (GRCm39)	missense	possibly damaging	0.92
R4943:Zfhx3	UTSW	8	109,674,949 (GRCm39)	missense	probably damaging	0.98
R5154:Zfhx3	UTSW	8	109,527,207 (GRCm39)	missense	probably damaging	1.00
R5377:Zfhx3	UTSW	8	109,677,817 (GRCm39)	missense	possibly damaging	0.95
R5388:Zfhx3	UTSW	8	109,673,446 (GRCm39)	missense	possibly damaging	0.88
R5434:Zfhx3	UTSW	8	109,519,031 (GRCm39)	missense	probably damaging	0.99
R5445:Zfhx3	UTSW	8	109,682,842 (GRCm39)	missense	unknown
R5541:Zfhx3	UTSW	8	109,675,583 (GRCm39)	missense	probably damaging	0.99
R5571:Zfhx3	UTSW	8	109,682,623 (GRCm39)	missense	unknown
R5700:Zfhx3	UTSW	8	109,660,499 (GRCm39)	missense	probably damaging	1.00
R5754:Zfhx3	UTSW	8	109,526,964 (GRCm39)	missense	probably damaging	0.99
R5867:Zfhx3	UTSW	8	109,520,078 (GRCm39)	missense	probably damaging	1.00
R5905:Zfhx3	UTSW	8	109,520,135 (GRCm39)	missense	probably damaging	1.00
R5922:Zfhx3	UTSW	8	109,673,330 (GRCm39)	missense	probably damaging	1.00
R5972:Zfhx3	UTSW	8	109,677,483 (GRCm39)	missense	possibly damaging	0.91
R6020:Zfhx3	UTSW	8	109,519,159 (GRCm39)	missense	probably damaging	1.00
R6028:Zfhx3	UTSW	8	109,520,135 (GRCm39)	missense	probably damaging	1.00
R6113:Zfhx3	UTSW	8	109,674,053 (GRCm39)	missense	probably benign	0.04
R6253:Zfhx3	UTSW	8	109,682,020 (GRCm39)	missense	possibly damaging	0.96
R6356:Zfhx3	UTSW	8	109,673,251 (GRCm39)	missense	probably damaging	1.00
R6800:Zfhx3	UTSW	8	109,676,149 (GRCm39)	missense	probably benign	0.20
R6829:Zfhx3	UTSW	8	109,676,915 (GRCm39)	missense	probably damaging	0.98
R6872:Zfhx3	UTSW	8	109,527,273 (GRCm39)	missense	probably damaging	1.00
R6873:Zfhx3	UTSW	8	109,527,273 (GRCm39)	missense	probably damaging	1.00
R6919:Zfhx3	UTSW	8	109,527,160 (GRCm39)	missense	probably damaging	1.00
R6921:Zfhx3	UTSW	8	109,678,024 (GRCm39)	missense	possibly damaging	0.53
R6925:Zfhx3	UTSW	8	109,683,453 (GRCm39)	missense	unknown
R6927:Zfhx3	UTSW	8	109,683,453 (GRCm39)	missense	unknown
R7152:Zfhx3	UTSW	8	109,674,839 (GRCm39)	missense	possibly damaging	0.94
R7169:Zfhx3	UTSW	8	109,678,030 (GRCm39)	missense	possibly damaging	0.86
R7214:Zfhx3	UTSW	8	109,675,493 (GRCm39)	missense	probably damaging	0.98
R7391:Zfhx3	UTSW	8	109,674,475 (GRCm39)	missense	probably damaging	0.96
R7442:Zfhx3	UTSW	8	109,519,468 (GRCm39)	missense	probably damaging	0.97
R7636:Zfhx3	UTSW	8	109,673,441 (GRCm39)	missense	probably benign	0.25
R7649:Zfhx3	UTSW	8	109,678,276 (GRCm39)	missense	probably benign	0.18
R7699:Zfhx3	UTSW	8	109,677,754 (GRCm39)	missense	probably benign	0.18
R7728:Zfhx3	UTSW	8	109,678,201 (GRCm39)	missense	probably benign	0.01
R7780:Zfhx3	UTSW	8	109,678,283 (GRCm39)	missense	possibly damaging	0.53
R7904:Zfhx3	UTSW	8	109,677,695 (GRCm39)	missense	probably damaging	0.98
R8032:Zfhx3	UTSW	8	109,677,854 (GRCm39)	missense	possibly damaging	0.51
R8158:Zfhx3	UTSW	8	109,675,353 (GRCm39)	missense	possibly damaging	0.82
R8163:Zfhx3	UTSW	8	109,675,925 (GRCm39)	missense	probably damaging	1.00
R8215:Zfhx3	UTSW	8	109,677,349 (GRCm39)	missense	probably benign
R8217:Zfhx3	UTSW	8	109,677,349 (GRCm39)	missense	probably benign
R8218:Zfhx3	UTSW	8	109,677,349 (GRCm39)	missense	probably benign
R8369:Zfhx3	UTSW	8	109,583,448 (GRCm39)	missense	possibly damaging	0.82
R8424:Zfhx3	UTSW	8	109,583,385 (GRCm39)	missense	probably damaging	0.98
R8482:Zfhx3	UTSW	8	109,674,511 (GRCm39)	missense	probably benign	0.02
R8504:Zfhx3	UTSW	8	109,583,549 (GRCm39)	missense	possibly damaging	0.95
R8871:Zfhx3	UTSW	8	109,676,867 (GRCm39)	missense	possibly damaging	0.85
R9144:Zfhx3	UTSW	8	109,676,794 (GRCm39)	missense	possibly damaging	0.53
R9202:Zfhx3	UTSW	8	109,677,920 (GRCm39)	missense	possibly damaging	0.92
R9213:Zfhx3	UTSW	8	109,676,756 (GRCm39)	missense	probably benign	0.18
R9218:Zfhx3	UTSW	8	109,520,501 (GRCm39)	missense	probably benign	0.17
R9370:Zfhx3	UTSW	8	109,521,340 (GRCm39)	missense	probably damaging	1.00
R9422:Zfhx3	UTSW	8	109,430,850 (GRCm39)	start gained	probably benign
R9530:Zfhx3	UTSW	8	109,527,010 (GRCm39)	missense	probably damaging	1.00
RF027:Zfhx3	UTSW	8	109,682,730 (GRCm39)	small insertion	probably benign
RF028:Zfhx3	UTSW	8	109,682,728 (GRCm39)	small insertion	probably benign
RF029:Zfhx3	UTSW	8	109,682,724 (GRCm39)	small insertion	probably benign
RF031:Zfhx3	UTSW	8	109,682,730 (GRCm39)	small insertion	probably benign
RF032:Zfhx3	UTSW	8	109,682,724 (GRCm39)	small insertion	probably benign
RF037:Zfhx3	UTSW	8	109,682,730 (GRCm39)	nonsense	probably null
RF038:Zfhx3	UTSW	8	109,682,733 (GRCm39)	small insertion	probably benign
RF040:Zfhx3	UTSW	8	109,682,733 (GRCm39)	small insertion	probably benign
RF042:Zfhx3	UTSW	8	109,682,730 (GRCm39)	small insertion	probably benign
RF042:Zfhx3	UTSW	8	109,682,720 (GRCm39)	small insertion	probably benign
RF054:Zfhx3	UTSW	8	109,682,728 (GRCm39)	small insertion	probably benign
RF060:Zfhx3	UTSW	8	109,682,720 (GRCm39)	small insertion	probably benign
X0019:Zfhx3	UTSW	8	109,678,285 (GRCm39)	missense	probably benign	0.00
X0026:Zfhx3	UTSW	8	109,675,777 (GRCm39)	missense	probably damaging	1.00
Z1088:Zfhx3	UTSW	8	109,677,989 (GRCm39)	missense	possibly damaging	0.72
Z1176:Zfhx3	UTSW	8	109,527,081 (GRCm39)	missense	probably damaging	1.00
Z1176:Zfhx3	UTSW	8	109,520,555 (GRCm39)	missense	probably benign	0.09

Predicted Primers

PCR Primer
(F):5'- ATGGCTTTGTGCTCTACGGC -3'
(R):5'- TAAGCCCGCCAAGGTTCAAG -3'

Sequencing Primer
(F):5'- CTACGGCAAGAGGAAACCCATTTTG -3'
(R):5'- TCAAGAGACCAGCCTGGG -3'

Posted On

2019-09-13