Mutagenetix > Incidental Mutation

Incidental Mutation 'R7392:Or2ag16'

573518

Institutional Source

Beutler Lab

Gene Symbol

Or2ag16

Ensembl Gene

ENSMUSG00000059087

Gene Name

olfactory receptor family 2 subfamily AG member 16

Synonyms

MOR283-3, GA_x6K02T2PBJ9-9130754-9129519, Olfr698

MMRRC Submission

045474-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.094) question?

Stock #

R7392 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

106351658-106352593 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 106352589 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 2 (E2G)

Ref Sequence

ENSEMBL: ENSMUSP00000149201 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000074981] [ENSMUST00000214306] [ENSMUST00000216255]

AlphaFold

Q7TRN4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000074981
AA Change: E2G

PolyPhen 2 Score 0.695 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000074509
Gene: ENSMUSG00000059087
AA Change: E2G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	9.4e-50	PFAM
Pfam:7TM_GPCR_Srsx	35	305	9.8e-8	PFAM
Pfam:7tm_1	41	290	4.1e-24	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000214306
AA Change: E2G

PolyPhen 2 Score 0.695 (Sensitivity: 0.86; Specificity: 0.92)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000216255
AA Change: E2G

PolyPhen 2 Score 0.695 (Sensitivity: 0.86; Specificity: 0.92)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 83 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610021A01Rik	T	A	7: 41,275,990 (GRCm39)	F564L	probably damaging	Het
Abcc2	T	C	19: 43,797,126 (GRCm39)	I499T	probably damaging	Het
Adamts13	C	T	2: 26,879,336 (GRCm39)	R630C	probably damaging	Het
Adgrv1	A	T	13: 81,708,808 (GRCm39)	F1199I	probably damaging	Het
Agbl5	G	A	5: 31,048,115 (GRCm39)		probably null	Het
Anks1b	A	G	10: 90,516,648 (GRCm39)	D881G	possibly damaging	Het
Arap2	A	T	5: 62,855,728 (GRCm39)	S569R	possibly damaging	Het
Arhgap15	T	C	2: 43,953,786 (GRCm39)	S171P	possibly damaging	Het
Arhgef11	G	A	3: 87,624,482 (GRCm39)		probably null	Het
Baz1a	C	A	12: 54,945,550 (GRCm39)	L1271F	probably damaging	Het
Bdnf	A	C	2: 109,554,275 (GRCm39)	K216N	probably benign	Het
Bmp7	C	T	2: 172,711,998 (GRCm39)	D409N	probably damaging	Het
Bnip5	A	T	17: 29,127,351 (GRCm39)	D219E	probably benign	Het
Cacna1c	T	A	6: 118,718,881 (GRCm39)	I390F		Het
Chd8	A	G	14: 52,470,312 (GRCm39)	S433P	probably benign	Het
Clrn2	A	G	5: 45,621,251 (GRCm39)	E215G	possibly damaging	Het
Col19a1	T	G	1: 24,573,115 (GRCm39)	D219A	unknown	Het
Col2a1	G	A	15: 97,878,032 (GRCm39)	R1036*	probably null	Het
Copg1	T	A	6: 87,867,257 (GRCm39)	V110D	probably benign	Het
Cpd	C	T	11: 76,692,605 (GRCm39)	G744D	probably damaging	Het
Crygs	G	A	16: 22,625,252 (GRCm39)	P63L	probably benign	Het
Dcn	A	G	10: 97,345,860 (GRCm39)	D224G	probably damaging	Het
Dnah7a	T	C	1: 53,540,820 (GRCm39)	E2518G	probably benign	Het
Efcab6	C	A	15: 83,873,152 (GRCm39)	R197L	probably benign	Het
Efr3b	T	A	12: 4,019,588 (GRCm39)	Y723F	probably benign	Het
Enam	A	G	5: 88,649,523 (GRCm39)	N344S	probably damaging	Het
Erich4	A	G	7: 25,315,101 (GRCm39)	I58T	possibly damaging	Het
Esrp1	A	G	4: 11,338,809 (GRCm39)	V665A	probably benign	Het
Etf1	G	A	18: 35,039,103 (GRCm39)	T388I	probably benign	Het
Faf1	A	G	4: 109,652,040 (GRCm39)	T244A	probably benign	Het
Fbn1	T	C	2: 125,185,844 (GRCm39)	D1610G	probably damaging	Het
Fcgbpl1	C	T	7: 27,863,797 (GRCm39)	T2523M	possibly damaging	Het
Frem1	G	T	4: 82,932,064 (GRCm39)	F212L	probably benign	Het
Fuom	T	A	7: 139,681,073 (GRCm39)	D85V	probably damaging	Het
Gcfc2	T	C	6: 81,919,993 (GRCm39)		probably null	Het
Gpr37	C	T	6: 25,688,786 (GRCm39)	A104T	probably benign	Het
Hsp90ab1	G	C	17: 45,879,974 (GRCm39)	T514S	probably benign	Het
Ifnl2	A	G	7: 28,209,094 (GRCm39)	F74L	probably benign	Het
Ipo11	G	A	13: 107,028,199 (GRCm39)	R367*	probably null	Het
Itpr2	T	C	6: 146,260,838 (GRCm39)	D963G	possibly damaging	Het
Krt15	A	T	11: 100,026,386 (GRCm39)	V100E	possibly damaging	Het
Lrch3	T	A	16: 32,807,125 (GRCm39)	L466*	probably null	Het
Lrfn5	A	T	12: 61,887,090 (GRCm39)	T293S	probably benign	Het
Lrp5	A	G	19: 3,660,199 (GRCm39)	I955T	probably damaging	Het
Lrrc4b	C	T	7: 44,111,439 (GRCm39)	T437M	probably damaging	Het
Man1a	A	G	10: 53,795,283 (GRCm39)	Y657H	probably damaging	Het
Mapkap1	G	T	2: 34,325,166 (GRCm39)	R94L	probably damaging	Het
Mtmr4	T	A	11: 87,495,383 (GRCm39)	L480Q	probably damaging	Het
Mycbp2	T	C	14: 103,389,627 (GRCm39)	I3504M	probably damaging	Het
Mycbp2	A	T	14: 103,480,564 (GRCm39)	C1169S	probably damaging	Het
Myo15a	T	C	11: 60,396,802 (GRCm39)	S1455P		Het
Niban1	A	G	1: 151,571,975 (GRCm39)	T307A	probably damaging	Het
Nid2	A	G	14: 19,818,724 (GRCm39)	D406G	probably benign	Het
Nrip2	A	G	6: 128,381,913 (GRCm39)	I69V	probably benign	Het
Nthl1	G	A	17: 24,857,598 (GRCm39)	V266I	probably benign	Het
Or5an6	G	T	19: 12,371,829 (GRCm39)	L67F	probably damaging	Het
Or5b21	A	G	19: 12,839,951 (GRCm39)	T271A	probably benign	Het
Or5g23	A	C	2: 85,438,832 (GRCm39)	C141G	possibly damaging	Het
Or5p75-ps1	C	A	7: 108,107,291 (GRCm39)	H9Q	possibly damaging	Het
Or8k25	A	G	2: 86,243,496 (GRCm39)	V300A	probably benign	Het
Or8s5	A	G	15: 98,238,192 (GRCm39)	V226A	probably benign	Het
Pcdhb1	A	C	18: 37,398,171 (GRCm39)	S41R	possibly damaging	Het
Pdcd11	T	A	19: 47,116,436 (GRCm39)	F1529I	probably damaging	Het
Plcd3	C	A	11: 102,992,383 (GRCm39)		probably benign	Het
Qdpr	T	C	5: 45,596,718 (GRCm39)	M149V	probably benign	Het
R3hcc1	T	A	14: 69,943,329 (GRCm39)		probably null	Het
Rasgrf2	T	C	13: 92,041,856 (GRCm39)	Y392C		Het
Slc8a3	A	C	12: 81,361,577 (GRCm39)	V414G	probably damaging	Het
Slco1a6	A	G	6: 142,103,003 (GRCm39)	S54P	probably benign	Het
Sos1	C	A	17: 80,731,629 (GRCm39)	V624F	probably damaging	Het
Spdef	T	C	17: 27,936,262 (GRCm39)	D227G	probably benign	Het
Sptb	G	T	12: 76,671,003 (GRCm39)	Q447K	probably damaging	Het
Srsf12	A	T	4: 33,209,265 (GRCm39)	R62W	unknown	Het
Stag3	T	C	5: 138,289,628 (GRCm39)	L266P	probably damaging	Het
Sult2b1	T	C	7: 45,391,862 (GRCm39)		probably benign	Het
Taf1a	A	G	1: 183,190,095 (GRCm39)	T66A		Het
Tfg	A	T	16: 56,532,972 (GRCm39)		probably null	Het
Trim34b	A	G	7: 103,985,604 (GRCm39)	N413S	probably benign	Het
Txndc15	T	A	13: 55,869,399 (GRCm39)	M184K	probably damaging	Het
Umodl1	A	C	17: 31,201,306 (GRCm39)	S412R	probably damaging	Het
Zbtb8b	T	A	4: 129,326,683 (GRCm39)	M161L	probably benign	Het
Zfp597	A	T	16: 3,684,369 (GRCm39)	V129E	probably benign	Het
Zfp790	T	C	7: 29,528,050 (GRCm39)	I245T	possibly damaging	Het

Other mutations in Or2ag16

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01373:Or2ag16	APN	7	106,351,653 (GRCm39)	utr 3 prime	probably benign
IGL01901:Or2ag16	APN	7	106,351,752 (GRCm39)	missense	possibly damaging	0.52
IGL01912:Or2ag16	APN	7	106,352,199 (GRCm39)	missense	probably damaging	1.00
IGL01998:Or2ag16	APN	7	106,351,758 (GRCm39)	missense	possibly damaging	0.63
IGL02640:Or2ag16	APN	7	106,352,559 (GRCm39)	missense	probably damaging	1.00
IGL03195:Or2ag16	APN	7	106,351,980 (GRCm39)	missense	probably benign
R0255:Or2ag16	UTSW	7	106,352,196 (GRCm39)	missense	probably benign	0.19
R1104:Or2ag16	UTSW	7	106,351,989 (GRCm39)	missense	probably benign	0.37
R1796:Or2ag16	UTSW	7	106,351,756 (GRCm39)	missense	probably benign	0.02
R1909:Or2ag16	UTSW	7	106,352,202 (GRCm39)	missense	probably benign	0.21
R4133:Or2ag16	UTSW	7	106,352,286 (GRCm39)	missense	probably damaging	0.98
R5194:Or2ag16	UTSW	7	106,352,426 (GRCm39)	missense	probably benign	0.15
R5389:Or2ag16	UTSW	7	106,352,290 (GRCm39)	missense	probably damaging	0.99
R5426:Or2ag16	UTSW	7	106,351,773 (GRCm39)	missense	probably benign
R6162:Or2ag16	UTSW	7	106,352,227 (GRCm39)	missense	probably damaging	1.00
R6463:Or2ag16	UTSW	7	106,352,008 (GRCm39)	missense	probably benign	0.23
R6643:Or2ag16	UTSW	7	106,351,776 (GRCm39)	missense	probably benign	0.41
R6831:Or2ag16	UTSW	7	106,351,778 (GRCm39)	missense	probably damaging	0.99
R6972:Or2ag16	UTSW	7	106,351,906 (GRCm39)	missense	possibly damaging	0.60
R7717:Or2ag16	UTSW	7	106,351,843 (GRCm39)	missense	possibly damaging	0.58
R7852:Or2ag16	UTSW	7	106,351,845 (GRCm39)	missense	probably damaging	0.98
R8073:Or2ag16	UTSW	7	106,352,008 (GRCm39)	nonsense	probably null
R8245:Or2ag16	UTSW	7	106,352,374 (GRCm39)	missense	probably benign	0.03
R8698:Or2ag16	UTSW	7	106,352,571 (GRCm39)	missense	probably benign	0.05
R9192:Or2ag16	UTSW	7	106,352,430 (GRCm39)	missense	probably damaging	1.00
R9333:Or2ag16	UTSW	7	106,351,782 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTCCATGAGAGAAAGCTGTCC -3'
(R):5'- TCAAATGTGGACTGATGGGATG -3'

Sequencing Primer
(F):5'- TCCATGAGAGAAAGCTGTCCAAGTAG -3'
(R):5'- GCCTTAAAAACATGTGCATATGAGC -3'

Posted On

2019-09-13