Mutagenetix > Incidental Mutations

Incidental Mutation 'R7393:Olfr620'

573599

Institutional Source

Beutler Lab

Gene Symbol

Olfr620

Ensembl Gene

ENSMUSG00000045132

Gene Name

olfactory receptor 620

Synonyms

MOR4-1, GA_x6K02T2PBJ9-6335095-6334154

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.094) question?

Stock #

R7393 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

103604895-103612534 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 103611991 bp

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 121 (S121P)

Ref Sequence

ENSEMBL: ENSMUSP00000061764 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052152] [ENSMUST00000217603]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000052152
AA Change: S121P

PolyPhen 2 Score 0.898 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000061764
Gene: ENSMUSG00000045132
AA Change: S121P

Domain	Start	End	E-Value	Type
Pfam:7tm_4	33	312	1.6e-137	PFAM
Pfam:7tm_1	43	294	4.5e-19	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000217603
AA Change: S121P

PolyPhen 2 Score 0.898 (Sensitivity: 0.82; Specificity: 0.94)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2010107E04Rik	A	G	12: 111,963,277	V26A	probably benign	Het
Abcb11	C	T	2: 69,299,867	D282N	probably damaging	Het
Abi2	T	C	1: 60,434,382	M86T	possibly damaging	Het
Adam26a	T	C	8: 43,569,688	N255S	probably benign	Het
Adam4	G	A	12: 81,419,660	S729L	probably benign	Het
Adcy1	G	T	11: 7,137,381	W418C	probably damaging	Het
Add2	T	A	6: 86,098,647	Y259*	probably null	Het
Agl	A	T	3: 116,791,156	C172S	probably benign	Het
Ankfy1	A	G	11: 72,738,308	T319A	possibly damaging	Het
Armc8	A	T	9: 99,483,999	C621S	possibly damaging	Het
Bbc3	A	G	7: 16,313,789	D146G	probably benign	Het
Btnl10	T	C	11: 58,923,706	L404P	probably damaging	Het
Cbl	A	G	9: 44,154,188		probably null	Het
Ccdc170	T	A	10: 4,514,314		probably null	Het
Ccdc91	T	A	6: 147,534,029	V37E	possibly damaging	Het
Ces1e	G	T	8: 93,210,417	T343K	probably benign	Het
Chfr	T	A	5: 110,152,358	F323I	probably damaging	Het
Clcnkb	T	A	4: 141,409,445	M370L	probably benign	Het
Col11a1	A	G	3: 114,097,106	D364G	unknown	Het
Ctbp2	A	G	7: 132,988,292	I381T	probably benign	Het
Cyp4a29	A	C	4: 115,242,196	Y38S	probably damaging	Het
Ddx1	T	C	12: 13,230,353	D382G	probably benign	Het
Dhx57	T	A	17: 80,255,571	N876Y	probably damaging	Het
Dsel	C	T	1: 111,861,573	G411S	probably damaging	Het
Enpp5	G	A	17: 44,085,264	G356S	probably damaging	Het
Fam234a	T	C	17: 26,216,624	D262G	probably benign	Het
Fnbp4	C	A	2: 90,779,316	Q1035K	probably damaging	Het
Gbp11	T	A	5: 105,327,577	N302Y	possibly damaging	Het
Gm11554	T	A	11: 99,803,872	T172S	unknown	Het
Hamp2	A	T	7: 30,922,605	M53K	possibly damaging	Het
Kcnj1	A	G	9: 32,397,018	D246G	probably damaging	Het
Kctd20	T	C	17: 28,963,338	F209L	probably damaging	Het
Klra2	T	C	6: 131,230,202	Y148C	probably damaging	Het
Krtap15	T	C	16: 88,829,097		probably null	Het
Mrps23	T	C	11: 88,204,458	L6P	probably damaging	Het
Ms4a6d	G	A	19: 11,590,073	Q155*	probably null	Het
Myo7a	G	A	7: 98,063,699	R1690C	possibly damaging	Het
Nbas	T	C	12: 13,393,492	S1183P	probably damaging	Het
Ndufaf4	T	A	4: 24,903,177	M122K	probably benign	Het
Nop2	C	A	6: 125,133,546	S45*	probably null	Het
Nop56	T	A	2: 130,274,638	L3Q	probably benign	Het
Nrp2	T	C	1: 62,745,424	I244T	probably damaging	Het
Olfr1426	T	A	19: 12,088,628	T55S	probably benign	Het
Otof	T	C	5: 30,370,270	D1941G	probably benign	Het
Pcyox1l	T	C	18: 61,697,641	K387E	probably benign	Het
Plcg2	A	G	8: 117,579,825	Y306C	possibly damaging	Het
Ppp1r13b	G	A	12: 111,838,754	P333S	probably damaging	Het
Rcc2	A	G	4: 140,717,030	D344G	probably damaging	Het
Reln	A	T	5: 21,976,351	N1810K	probably damaging	Het
Rfesd	G	T	13: 76,003,030	A90E	probably benign	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,579,917		probably benign	Het
Scg2	T	C	1: 79,435,231	K552E	probably damaging	Het
Sdccag8	A	G	1: 176,840,306	I190V	probably benign	Het
Sele	C	A	1: 164,053,923	T533K	probably benign	Het
Sh3bp4	T	A	1: 89,144,448	H339Q	possibly damaging	Het
Slc6a19	A	G	13: 73,692,974	S106P	probably benign	Het
Spidr	C	T	16: 16,146,831		probably benign	Het
Stk36	A	T	1: 74,611,193	K295*	probably null	Het
Synj1	A	T	16: 90,951,999	D1056E	probably damaging	Het
Tnks1bp1	T	C	2: 85,062,866	S1046P	probably benign	Het
Ttc27	T	G	17: 74,770,264	F385V	possibly damaging	Het
Ttn	C	A	2: 76,944,345	G2164W	unknown	Het
Ubap1	T	A	4: 41,379,764	L326*	probably null	Het
Ubr4	A	G	4: 139,426,785	N812S	probably damaging	Het
Vmn1r160	A	G	7: 22,871,353	T44A	possibly damaging	Het
Vmn1r28	G	A	6: 58,265,589	S139N	possibly damaging	Het
Vmn2r116	A	G	17: 23,386,125	I137M	probably benign	Het
Vmn2r67	A	T	7: 85,155,878	W9R	probably null	Het
Zfp101	T	C	17: 33,386,700	N45D	possibly damaging	Het

Other mutations in Olfr620

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02807:Olfr620	APN	7	103611991	missense	probably benign	0.34
R0496:Olfr620	UTSW	7	103611997	missense	probably benign	0.09
R0834:Olfr620	UTSW	7	103612237	missense	probably benign	0.00
R1957:Olfr620	UTSW	7	103611411	makesense	probably null
R1958:Olfr620	UTSW	7	103611411	makesense	probably null
R2158:Olfr620	UTSW	7	103612236	missense	possibly damaging	0.66
R3797:Olfr620	UTSW	7	103611447	missense	probably benign	0.37
R5389:Olfr620	UTSW	7	103611590	nonsense	probably null
R6292:Olfr620	UTSW	7	103612179	missense	probably damaging	0.99
R6374:Olfr620	UTSW	7	103611921	missense	probably benign	0.23
R6422:Olfr620	UTSW	7	103612014	missense	probably damaging	1.00
R6586:Olfr620	UTSW	7	103611976	missense	possibly damaging	0.81
R7234:Olfr620	UTSW	7	103611882	missense	probably damaging	0.97
R7855:Olfr620	UTSW	7	103611772	missense	possibly damaging	0.88
R7900:Olfr620	UTSW	7	103611693	missense	possibly damaging	0.82
R8159:Olfr620	UTSW	7	103612140	missense	possibly damaging	0.63
R8265:Olfr620	UTSW	7	103611841	missense	possibly damaging	0.95
R8314:Olfr620	UTSW	7	103612047	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTCTTGGTGCAGGCAGAAAG -3'
(R):5'- TGATTCGGACTGAGCTGAGC -3'

Sequencing Primer
(F):5'- CTTGGTGCAGGCAGAAAGAATGG -3'
(R):5'- ACTGAGCTGAGCCTGCAC -3'

Posted On

2019-09-13