|Institutional Source||Beutler Lab|
|Gene Name||G protein-coupled receptor kinase 4|
|Is this an essential gene?||Probably non essential (E-score: 0.148)|
|Stock #||R7394 (G1)|
|Chromosomal Location||34660379-34755305 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 34751618 bp|
|Amino Acid Change||Asparagine to Tyrosine at position 490 (N490Y)|
|Ref Sequence||ENSEMBL: ENSMUSP00000001112 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000001112]|
|Predicted Effect||probably benign
AA Change: N490Y
PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)
AA Change: N490Y
|Meta Mutation Damage Score||0.0846|
|Coding Region Coverage||
|Validation Efficiency||99% (66/67)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
PHENOTYPE: Mice heterozygous for a knock-out allele are viable, fertile and overtly normal. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Grk4||
(F):5'- ATTAAGGGAGCTCAGAGTTGC -3'
(R):5'- GAATAGCTGCCATGCTTCTGG -3'
(F):5'- GGTGCTTTCCCAACATATGCAGG -3'
(R):5'- GCATAGTCTTGATTTACCTGTGATC -3'