Mutagenetix > Incidental Mutation

Incidental Mutation 'R7394:Zfp651'

573673

Institutional Source

Beutler Lab

Gene Symbol

Zfp651

Ensembl Gene

ENSMUSG00000013419

Gene Name

zinc finger protein 651

Synonyms

4732420M22Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.122) question?

Stock #

R7394 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

121759330-121771742 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 121767345 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 626 (M626K)

Ref Sequence

ENSEMBL: ENSMUSP00000091286 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000093772]

AlphaFold

E9PZ11

Predicted Effect

probably damaging
Transcript: ENSMUST00000093772
AA Change: M626K

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000091286
Gene: ENSMUSG00000013419
AA Change: M626K

Domain	Start	End	E-Value	Type
BTB	45	141	3.69e-19	SMART
low complexity region	159	164	N/A	INTRINSIC
low complexity region	197	210	N/A	INTRINSIC
low complexity region	217	231	N/A	INTRINSIC
low complexity region	302	339	N/A	INTRINSIC
low complexity region	355	372	N/A	INTRINSIC
ZnF_C2H2	447	470	1.28e-3	SMART
ZnF_C2H2	474	494	8.4e1	SMART
ZnF_C2H2	501	524	1.76e-1	SMART
ZnF_C2H2	531	553	3.34e-2	SMART
ZnF_C2H2	559	581	6.78e-3	SMART
ZnF_C2H2	587	609	3.63e-3	SMART
ZnF_C2H2	615	637	1.95e-3	SMART
ZnF_C2H2	643	665	6.62e-6	SMART
ZnF_C2H2	671	698	4.16e1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000214732

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

99% (66/67)

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700017N19Rik	A	G	10: 100,609,176	I208V	probably benign	Het
Abcb11	C	T	2: 69,299,867	D282N	probably damaging	Het
Aldh1l2	T	A	10: 83,502,457	I646F	probably damaging	Het
Alms1	C	T	6: 85,622,223	P1344S	possibly damaging	Het
Ank2	T	A	3: 126,936,653	I711L	possibly damaging	Het
Ankrd6	T	C	4: 32,821,298	N251D	probably damaging	Het
Ap3m1	T	C	14: 21,038,079	T304A	probably benign	Het
Arhgef39	T	C	4: 43,499,532	T26A	possibly damaging	Het
C530008M17Rik	GCGCGAGGCCGAGAGGCAGGAGGAGGAAGCAAGACAACGCGAGGCCGAGAGGCAGG	GCGCGAGGCCGAGAGGCAGG	5: 76,856,954		probably benign	Het
Carnmt1	G	T	19: 18,670,837		probably benign	Het
Ccr4	C	T	9: 114,491,926	R357H	probably benign	Het
Cd4	T	A	6: 124,873,041	M104L	probably benign	Het
Cd74	A	T	18: 60,803,893		probably benign	Het
Cdcp1	T	C	9: 123,173,813	Y731C	probably damaging	Het
Cdyl2	A	G	8: 116,624,051	S114P	not run	Het
Cenpv	T	C	11: 62,536,288	D148G	probably damaging	Het
Cep89	G	A	7: 35,429,928	R630H	probably damaging	Het
Cfap57	T	A	4: 118,593,137	Y596F	probably benign	Het
Clec4a2	C	A	6: 123,139,120	A122E	unknown	Het
Col4a2	A	G	8: 11,446,184	T1602A	probably benign	Het
Cyp2j11	A	T	4: 96,316,440	Y290N	probably benign	Het
Dhx38	A	T	8: 109,556,523	V554E	probably damaging	Het
Ebf2	T	C	14: 67,237,526	V70A	probably damaging	Het
Enpp5	G	A	17: 44,085,264	G356S	probably damaging	Het
Fam217a	A	T	13: 34,910,279	I499K	possibly damaging	Het
Fras1	C	A	5: 96,712,450	Y2118*	probably null	Het
Gm3248	A	T	14: 5,945,781		probably null	Het
Gm5460	T	G	14: 34,043,922	D165E	possibly damaging	Het
Grk4	A	T	5: 34,751,618	N490Y	probably benign	Het
Iglc2	T	A	16: 19,195,136	K59*	probably null	Het
Iqsec3	T	A	6: 121,386,610	H895L	possibly damaging	Het
Itgb7	A	G	15: 102,219,254	S410P	probably damaging	Het
Kmt2d	C	A	15: 98,856,384	V1613F	unknown	Het
Lama1	T	C	17: 67,717,261	L118P		Het
Lrrc25	A	T	8: 70,618,180	S204C	possibly damaging	Het
Malrd1	A	G	2: 15,695,199	D619G	unknown	Het
Ms4a6d	G	A	19: 11,590,073	Q155*	probably null	Het
Mup17	G	A	4: 61,594,398	S86F	probably benign	Het
Nbeal2	C	T	9: 110,630,189		probably null	Het
Nfkb1	A	C	3: 135,613,697	V291G	possibly damaging	Het
Nomo1	G	T	7: 46,066,479	V757F	probably benign	Het
Nutm2	T	A	13: 50,470,007	S247T	probably damaging	Het
Olfr1225	C	T	2: 89,170,361	V284I	probably benign	Het
Olfr1291-ps1	G	T	2: 111,499,896	A215S	probably damaging	Het
Olfr521	C	A	7: 99,767,346	H61Q	probably damaging	Het
Olfr826	T	A	10: 130,180,254	I209F	probably damaging	Het
Olfr834	T	A	9: 18,988,710	C241S	probably damaging	Het
Pcdhb14	A	G	18: 37,448,908	I356V	probably benign	Het
Pnkp	T	A	7: 44,858,678	S142T	probably damaging	Het
Ppia	T	C	11: 6,419,218	S99P	possibly damaging	Het
Prss47	C	T	13: 65,044,993	V325I	probably benign	Het
Ptk7	T	C	17: 46,591,757	D34G	probably damaging	Het
Pwp2	C	T	10: 78,182,480	G126R	probably damaging	Het
Rasa3	G	T	8: 13,595,353	D195E	probably benign	Het
Rnf150	T	A	8: 82,990,471	Y202*	probably null	Het
Sh2d1b2	T	C	1: 170,248,147	V50A	probably damaging	Het
Slc30a4	C	T	2: 122,685,304	V390I	possibly damaging	Het
Slc30a9	G	T	5: 67,352,766		probably null	Het
Slc8a3	T	A	12: 81,214,058		probably null	Het
Smpd3	G	A	8: 106,265,010	R304W	probably damaging	Het
Snta1	A	G	2: 154,376,860	S490P	probably damaging	Het
Srcap	T	G	7: 127,534,828	M887R	probably damaging	Het
St3gal1	A	G	15: 67,111,346	V187A	possibly damaging	Het
Sult2a3	T	C	7: 14,111,524	T137A	probably benign	Het
Tspoap1	C	T	11: 87,766,119	Q367*	probably null	Het
Uroc1	C	T	6: 90,345,333	R280C	probably damaging	Het
Ush2a	T	C	1: 188,911,416	I4325T	possibly damaging	Het
Vmn1r32	A	G	6: 66,553,189	I201T	probably benign	Het
Zadh2	C	T	18: 84,088,190	A9V	probably benign	Het

Other mutations in Zfp651

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01913:Zfp651	APN	9	121763969	missense	probably damaging	1.00
R0193:Zfp651	UTSW	9	121767666	missense	probably damaging	0.98
R0270:Zfp651	UTSW	9	121767575	missense	probably benign	0.27
R0347:Zfp651	UTSW	9	121763102	missense	probably damaging	1.00
R0654:Zfp651	UTSW	9	121763261	missense	probably benign	0.01
R2202:Zfp651	UTSW	9	121762637	missense	possibly damaging	0.53
R2203:Zfp651	UTSW	9	121762637	missense	possibly damaging	0.53
R2204:Zfp651	UTSW	9	121762637	missense	possibly damaging	0.53
R2205:Zfp651	UTSW	9	121762637	missense	possibly damaging	0.53
R2364:Zfp651	UTSW	9	121767594	missense	probably damaging	0.98
R3843:Zfp651	UTSW	9	121763433	missense	possibly damaging	0.86
R4275:Zfp651	UTSW	9	121766539	missense	probably damaging	1.00
R4934:Zfp651	UTSW	9	121763979	missense	probably damaging	0.99
R5358:Zfp651	UTSW	9	121765595	missense	probably damaging	1.00
R5462:Zfp651	UTSW	9	121767663	missense	probably damaging	0.99
R5613:Zfp651	UTSW	9	121767519	missense	probably damaging	1.00
R5843:Zfp651	UTSW	9	121767339	missense	possibly damaging	0.47
R5863:Zfp651	UTSW	9	121767530	missense	probably benign	0.22
R6009:Zfp651	UTSW	9	121762871	missense	possibly damaging	0.86
R6063:Zfp651	UTSW	9	121763532	missense	probably benign	0.01
R6114:Zfp651	UTSW	9	121765595	missense	probably damaging	1.00
R6223:Zfp651	UTSW	9	121763787	missense	possibly damaging	0.61
R6414:Zfp651	UTSW	9	121763659	missense	probably benign
R6811:Zfp651	UTSW	9	121766529	missense	probably damaging	1.00
R7430:Zfp651	UTSW	9	121763666	missense	probably benign	0.02
R8215:Zfp651	UTSW	9	121767278	missense	probably benign	0.40
R8900:Zfp651	UTSW	9	121767639	missense	probably damaging	0.99
R8982:Zfp651	UTSW	9	121763268	missense	probably benign	0.26
R9623:Zfp651	UTSW	9	121762924	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- CTTAGGAAACACTGGCCTACAC -3'
(R):5'- GTCGATGCCGCTTCATATTGG -3'

Sequencing Primer
(F):5'- ACTGGCCTACACACAGGTTG -3'
(R):5'- TGGTGAAACTCTTGCCACAG -3'

Posted On

2019-09-13