Mutagenetix > Incidental Mutation

Incidental Mutation 'R7398:Mrgpra9'

573964

Institutional Source

Beutler Lab

Gene Symbol

Mrgpra9

Ensembl Gene

ENSMUSG00000074111

Gene Name

MAS-related GPR, member A9

Synonyms

MrgA9, EG668725

MMRRC Submission

045480-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R7398 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

46884667-46902627 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 46885385 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 94 (I94T)

Ref Sequence

ENSEMBL: ENSMUSP00000136396 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098436] [ENSMUST00000179005]

AlphaFold

A0A140T8U8

Predicted Effect

possibly damaging
Transcript: ENSMUST00000098436
AA Change: I93T

PolyPhen 2 Score 0.681 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000096035
Gene: ENSMUSG00000074111
AA Change: I93T

Domain	Start	End	E-Value	Type
low complexity region	37	48	N/A	INTRINSIC
Pfam:7tm_1	56	225	1.5e-11	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000179005
AA Change: I94T

PolyPhen 2 Score 0.681 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000136396
Gene: ENSMUSG00000074111
AA Change: I94T

Domain	Start	End	E-Value	Type
Pfam:7tm_1	12	178	3.4e-9	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abl1	T	A	2: 31,680,811 (GRCm39)	S368T	probably damaging	Het
Acmsd	A	G	1: 127,657,172 (GRCm39)		probably benign	Het
Arid3b	A	T	9: 57,703,495 (GRCm39)	S445T	probably benign	Het
Brinp1	A	G	4: 68,759,591 (GRCm39)	V91A	probably benign	Het
Ccne1	A	G	7: 37,805,702 (GRCm39)		probably null	Het
Col3a1	A	T	1: 45,366,973 (GRCm39)	I249F	unknown	Het
Crybg3	T	C	16: 59,377,688 (GRCm39)	I1189V	probably benign	Het
Cyp2d34	A	T	15: 82,500,964 (GRCm39)	D389E	probably benign	Het
D130043K22Rik	A	C	13: 25,077,360 (GRCm39)	I998L	probably damaging	Het
Dgkq	A	G	5: 108,803,056 (GRCm39)	I298T	possibly damaging	Het
Dnah17	T	C	11: 117,971,550 (GRCm39)	E2161G	probably damaging	Het
Dnajc25	T	C	4: 59,017,824 (GRCm39)		probably null	Het
Dpp9	G	A	17: 56,496,405 (GRCm39)	R768*	probably null	Het
Dusp6	T	A	10: 99,100,740 (GRCm39)	N245K	probably damaging	Het
Eea1	C	A	10: 95,831,493 (GRCm39)	H195N	probably benign	Het
Efhc1	A	G	1: 21,059,744 (GRCm39)	E598G	probably benign	Het
Epb41l1	T	C	2: 156,376,682 (GRCm39)	V809A	probably damaging	Het
Gas8	T	A	8: 124,245,690 (GRCm39)	M1K	probably null	Het
Gcm1	G	A	9: 77,971,961 (GRCm39)	V301I	probably benign	Het
Gm11554	T	A	11: 99,695,085 (GRCm39)	S43C	unknown	Het
Gm7298	A	G	6: 121,758,912 (GRCm39)	I1177V	probably benign	Het
Gm8011	A	G	14: 42,285,918 (GRCm39)	T27A		Het
Hmcn1	A	G	1: 150,522,421 (GRCm39)	I3493T	probably benign	Het
Insrr	T	G	3: 87,716,039 (GRCm39)	I578S	probably damaging	Het
Kif13b	A	G	14: 64,994,972 (GRCm39)	D908G	probably null	Het
Lhcgr	G	C	17: 89,079,474 (GRCm39)	Q71E	probably benign	Het
Lrrc34	T	C	3: 30,697,491 (GRCm39)	D80G	probably damaging	Het
Lrrc7	C	A	3: 157,997,595 (GRCm39)	E156*	probably null	Het
Lrrc9	A	G	12: 72,547,590 (GRCm39)	D1255G	probably damaging	Het
Mcidas	A	G	13: 113,133,416 (GRCm39)	N116D	probably benign	Het
Mmp7	A	G	9: 7,697,594 (GRCm39)	N210D	probably damaging	Het
Morc3	A	G	16: 93,671,748 (GRCm39)	D926G	probably damaging	Het
Muc16	C	A	9: 18,549,038 (GRCm39)	V5752F	possibly damaging	Het
Myo16	A	T	8: 10,612,183 (GRCm39)	D1276V	unknown	Het
Nlrc4	T	G	17: 74,753,537 (GRCm39)	E282A	probably damaging	Het
Nos2	T	A	11: 78,827,297 (GRCm39)	Y227*	probably null	Het
Obox5	A	C	7: 15,492,713 (GRCm39)	M223L	probably benign	Het
Or10ak13	C	A	4: 118,638,896 (GRCm39)	L295F	possibly damaging	Het
Pygm	G	A	19: 6,435,966 (GRCm39)	R139H	probably damaging	Het
Ranbp2	C	A	10: 58,303,099 (GRCm39)	P789T	probably damaging	Het
Riok2	T	G	17: 17,607,501 (GRCm39)	S350A	probably benign	Het
Rnf214	C	T	9: 45,778,845 (GRCm39)	V445I	possibly damaging	Het
Skida1	C	T	2: 18,051,083 (GRCm39)	V603I	unknown	Het
Skint6	T	A	4: 112,755,335 (GRCm39)	R747S	probably benign	Het
Slc24a5	G	A	2: 124,927,694 (GRCm39)	W331*	probably null	Het
Slc5a10	A	T	11: 61,564,405 (GRCm39)	C525S	probably benign	Het
Styxl2	A	G	1: 165,928,044 (GRCm39)	S523P	probably damaging	Het
Sult2b1	T	C	7: 45,380,718 (GRCm39)	Y288C	probably damaging	Het
Tagln3	G	T	16: 45,543,440 (GRCm39)	N67K	probably damaging	Het
Trav21-dv12	A	T	14: 54,114,162 (GRCm39)	H94L	probably benign	Het
Tshz2	A	G	2: 169,726,094 (GRCm39)	E230G	probably damaging	Het
Usb1	T	A	8: 96,071,931 (GRCm39)	H210Q	probably damaging	Het
Vill	C	T	9: 118,899,716 (GRCm39)	P767L	probably benign	Het
Vmn2r94	A	T	17: 18,477,603 (GRCm39)	N269K	probably benign	Het
Zfp764l1	C	T	7: 126,992,496 (GRCm39)	C38Y	probably null	Het

Other mutations in Mrgpra9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Mrgpra9	APN	7	46,884,839 (GRCm39)	missense	possibly damaging	0.85
IGL00575:Mrgpra9	APN	7	46,885,053 (GRCm39)	missense	possibly damaging	0.76
IGL01649:Mrgpra9	APN	7	46,884,900 (GRCm39)	missense	probably benign	0.22
IGL03207:Mrgpra9	APN	7	46,885,385 (GRCm39)	missense	possibly damaging	0.68
R0388:Mrgpra9	UTSW	7	46,902,542 (GRCm39)	start codon destroyed	probably null	0.08
R0972:Mrgpra9	UTSW	7	46,885,203 (GRCm39)	missense	probably damaging	0.99
R1270:Mrgpra9	UTSW	7	46,902,531 (GRCm39)	critical splice donor site	probably null
R1381:Mrgpra9	UTSW	7	46,885,050 (GRCm39)	missense	possibly damaging	0.75
R1403:Mrgpra9	UTSW	7	46,885,386 (GRCm39)	missense	probably benign	0.24
R1403:Mrgpra9	UTSW	7	46,885,386 (GRCm39)	missense	probably benign	0.24
R1448:Mrgpra9	UTSW	7	46,885,561 (GRCm39)	missense	probably benign	0.02
R2045:Mrgpra9	UTSW	7	46,885,583 (GRCm39)	missense	probably benign
R2144:Mrgpra9	UTSW	7	46,885,211 (GRCm39)	missense	probably benign	0.31
R2187:Mrgpra9	UTSW	7	46,884,797 (GRCm39)	missense	probably damaging	1.00
R2507:Mrgpra9	UTSW	7	46,885,242 (GRCm39)	missense	possibly damaging	0.63
R2913:Mrgpra9	UTSW	7	46,884,828 (GRCm39)	missense	probably benign
R3810:Mrgpra9	UTSW	7	46,885,527 (GRCm39)	missense	probably damaging	0.98
R4177:Mrgpra9	UTSW	7	46,885,302 (GRCm39)	missense	probably damaging	1.00
R4521:Mrgpra9	UTSW	7	46,884,938 (GRCm39)	missense	probably damaging	1.00
R4781:Mrgpra9	UTSW	7	46,884,795 (GRCm39)	missense	possibly damaging	0.88
R4926:Mrgpra9	UTSW	7	46,884,759 (GRCm39)	missense	possibly damaging	0.62
R6469:Mrgpra9	UTSW	7	46,884,854 (GRCm39)	missense	probably benign	0.02
R6505:Mrgpra9	UTSW	7	46,884,884 (GRCm39)	missense	probably benign	0.00
R6724:Mrgpra9	UTSW	7	46,884,786 (GRCm39)	missense	probably damaging	1.00
R8737:Mrgpra9	UTSW	7	46,885,624 (GRCm39)	missense	probably benign	0.00
R8824:Mrgpra9	UTSW	7	46,885,041 (GRCm39)	missense	probably benign	0.06
R8881:Mrgpra9	UTSW	7	46,885,242 (GRCm39)	missense	possibly damaging	0.63
R9320:Mrgpra9	UTSW	7	46,885,392 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCAGCACACATGACAGTTGATG -3'
(R):5'- ATGGACGAAACCATCCCTGG -3'

Sequencing Primer
(F):5'- CACACATGACAGTTGATGTGTGTTC -3'
(R):5'- TGGAAGTACCAGCATCAAGATTCTG -3'

Posted On

2019-09-13