Mutagenetix > Incidental Mutation

Incidental Mutation 'R0625:Cyp4f37'

57445

Institutional Source

Beutler Lab

Gene Symbol

Cyp4f37

Ensembl Gene

ENSMUSG00000062464

Gene Name

cytochrome P450, family 4, subfamily f, polypeptide 37

Synonyms

Gm9705

MMRRC Submission

038814-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.249) question?

Stock #

R0625 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

32621319-32636184 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 32634678 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 445 (F445L)

Ref Sequence

ENSEMBL: ENSMUSP00000076827 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077639]

AlphaFold

Q3V1F1

Predicted Effect

probably damaging
Transcript: ENSMUST00000077639
AA Change: F445L

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000076827
Gene: ENSMUSG00000062464
AA Change: F445L

Domain	Start	End	E-Value	Type
Pfam:p450	52	515	1.1e-136	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.5%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930550C14Rik	T	C	9: 53,408,065	S2P	probably benign	Het
Abca16	A	C	7: 120,435,893	T301P	probably damaging	Het
Acer2	A	G	4: 86,887,162	D121G	possibly damaging	Het
Adgrd1	T	C	5: 129,171,931		probably null	Het
Arhgap11a	T	C	2: 113,841,711	I249V	probably benign	Het
Arhgap22	A	G	14: 33,366,714	E219G	probably benign	Het
C2cd4b	T	A	9: 67,759,751	S10T	probably benign	Het
Cnot6	A	T	11: 49,683,171	I224N	probably damaging	Het
Ctrc	T	C	4: 141,841,518	T125A	probably damaging	Het
Cxxc5	T	G	18: 35,858,589	S14R	unknown	Het
Dcbld1	T	G	10: 52,312,850	I186S	probably benign	Het
Dmxl2	T	C	9: 54,382,702	T2510A	probably benign	Het
Dnah3	A	G	7: 120,071,887	I591T	possibly damaging	Het
Dock5	A	T	14: 67,841,163	I204N	probably benign	Het
Dysf	G	A	6: 84,111,987		probably null	Het
Erich5	A	G	15: 34,471,369	E248G	probably damaging	Het
Fam160a1	A	G	3: 85,730,500	V164A	possibly damaging	Het
Foxm1	A	G	6: 128,373,871	S712G	probably damaging	Het
Frmpd1	A	G	4: 45,284,055	T959A	probably benign	Het
Gfra4	C	T	2: 131,040,256	V277I	probably null	Het
Hacd4	T	C	4: 88,435,010	I82V	probably benign	Het
Itih2	C	T	2: 10,123,414	V159I	possibly damaging	Het
Itpr2	T	A	6: 146,166,651	M2410L	probably benign	Het
March11	A	G	15: 26,311,043	I202V	probably damaging	Het
March3	A	G	18: 56,811,830		probably null	Het
Med12l	G	A	3: 59,247,437	E1135K	probably damaging	Het
Mib2	C	T	4: 155,659,460	G42S	probably damaging	Het
Mlx	T	C	11: 101,087,782	L78P	possibly damaging	Het
Muc5b	T	C	7: 141,846,427	C473R	unknown	Het
N4bp2l1	T	A	5: 150,576,745	R66*	probably null	Het
Nes	A	G	3: 87,977,172	T913A	possibly damaging	Het
Oas1a	T	C	5: 120,899,259	E235G	probably damaging	Het
Olfr1104	T	A	2: 87,021,620	H308L	probably benign	Het
Olfr477	T	C	7: 107,991,189	S275P	probably damaging	Het
Olfr905	T	C	9: 38,473,208	S154P	possibly damaging	Het
Parn	C	T	16: 13,640,294	V286I	probably benign	Het
Paxip1	G	A	5: 27,765,942	Q470*	probably null	Het
Phc2	C	G	4: 128,723,710	H510D	possibly damaging	Het
Pla2g4f	T	A	2: 120,305,041	D384V	probably damaging	Het
Plpbp	A	T	8: 27,045,131	N68I	probably damaging	Het
Podxl2	G	A	6: 88,849,955	A123V	possibly damaging	Het
Pole	A	T	5: 110,325,550	T1737S	possibly damaging	Het
Ppp3cc	T	C	14: 70,225,027	E396G	probably damaging	Het
Pramel7	T	A	2: 87,491,008	I228F	probably benign	Het
Prl7d1	A	T	13: 27,710,140	C149S	probably benign	Het
Qtrt1	G	T	9: 21,418,288	M217I	probably benign	Het
Sec24a	T	A	11: 51,729,454	D456V	probably damaging	Het
Shox2	T	G	3: 66,981,544		probably null	Het
Skint2	T	A	4: 112,624,086	S49T	probably damaging	Het
Smarca5	A	G	8: 80,720,686		probably null	Het
Sorcs2	T	A	5: 36,024,572	D1068V	possibly damaging	Het
Tmem114	T	C	16: 8,412,102		probably null	Het
Ttc7b	T	A	12: 100,355,046	M24L	probably benign	Het
Ttll3	A	G	6: 113,408,903		probably null	Het
Usp7	C	T	16: 8,704,982	D102N	probably benign	Het

Other mutations in Cyp4f37

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01140:Cyp4f37	APN	17	32629053	missense	probably benign	0.20
IGL01994:Cyp4f37	APN	17	32625176	nonsense	probably null
IGL02073:Cyp4f37	APN	17	32627851	missense	possibly damaging	0.59
IGL02145:Cyp4f37	APN	17	32630035	missense	probably benign	0.43
IGL02814:Cyp4f37	APN	17	32634671	missense	probably benign	0.01
IGL02873:Cyp4f37	APN	17	32625168	missense	probably benign	0.00
IGL02937:Cyp4f37	APN	17	32625189	missense	probably benign	0.00
IGL03170:Cyp4f37	APN	17	32625119	splice site	probably benign
R1774:Cyp4f37	UTSW	17	32629890	missense	possibly damaging	0.59
R1871:Cyp4f37	UTSW	17	32634665	missense	probably damaging	1.00
R2232:Cyp4f37	UTSW	17	32634270	missense	probably benign	0.23
R2847:Cyp4f37	UTSW	17	32629125	missense	probably damaging	1.00
R2848:Cyp4f37	UTSW	17	32629125	missense	probably damaging	1.00
R4027:Cyp4f37	UTSW	17	32631672	missense	probably benign	0.00
R4463:Cyp4f37	UTSW	17	32627736	critical splice acceptor site	probably null
R4517:Cyp4f37	UTSW	17	32631592	missense	probably benign	0.00
R4573:Cyp4f37	UTSW	17	32629087	missense	probably benign	0.23
R4670:Cyp4f37	UTSW	17	32625152	missense	probably benign
R5752:Cyp4f37	UTSW	17	32631332	missense	probably damaging	1.00
R5930:Cyp4f37	UTSW	17	32629983	missense	possibly damaging	0.79
R6248:Cyp4f37	UTSW	17	32629890	missense	possibly damaging	0.59
R7412:Cyp4f37	UTSW	17	32629844	missense	possibly damaging	0.87
R7969:Cyp4f37	UTSW	17	32625207	missense	probably benign	0.00
R8066:Cyp4f37	UTSW	17	32635073	missense	probably benign	0.21
R8187:Cyp4f37	UTSW	17	32635197	missense	probably benign
R8303:Cyp4f37	UTSW	17	32634178	critical splice acceptor site	probably null
R8309:Cyp4f37	UTSW	17	32634978	missense	probably damaging	1.00
R8407:Cyp4f37	UTSW	17	32634184	missense	probably damaging	1.00
R8869:Cyp4f37	UTSW	17	32625122	missense	probably benign	0.01
R9054:Cyp4f37	UTSW	17	32634279	missense	probably benign	0.25
R9565:Cyp4f37	UTSW	17	32625231	missense	possibly damaging	0.79
R9674:Cyp4f37	UTSW	17	32627867	critical splice donor site	probably null
X0057:Cyp4f37	UTSW	17	32625224	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- CTACCCAACAGGGAACATCTGTGTC -3'
(R):5'- TGCCACCATCATCTCATTCATAGCG -3'

Sequencing Primer
(F):5'- GGAACATCTGTGTCATCAGCATC -3'
(R):5'- AGCGAAAGTCTGTCCTATGC -3'

Posted On

2013-07-11