Mutagenetix > Incidental Mutations

Incidental Mutation 'R7236:Tmem260'

574611

Institutional Source

Beutler Lab

Gene Symbol

Tmem260

Ensembl Gene

ENSMUSG00000036339

Gene Name

transmembrane protein 260

Synonyms

6720456H20Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7236 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

48446124-48524246 bp(+) (GRCm38)

Type of Mutation

splice site (65 bp from exon)

DNA Base Change (assembly)

C to T at 48509190 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000154360 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000111735] [ENSMUST00000124720] [ENSMUST00000153765] [ENSMUST00000226422] [ENSMUST00000227440]

AlphaFold

Q8BMD6

Predicted Effect

probably null
Transcript: ENSMUST00000111735

SMART Domains

Protein: ENSMUSP00000107364
Gene: ENSMUSG00000036339

Domain	Start	End	E-Value	Type
transmembrane domain	21	40	N/A	INTRINSIC
Pfam:DUF2723	48	211	1.9e-43	PFAM
transmembrane domain	316	338	N/A	INTRINSIC
transmembrane domain	353	372	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000124720

SMART Domains

Protein: ENSMUSP00000118376
Gene: ENSMUSG00000036339

Domain	Start	End	E-Value	Type
Pfam:DUF2723	1	61	4.6e-9	PFAM
transmembrane domain	65	87	N/A	INTRINSIC
transmembrane domain	164	186	N/A	INTRINSIC
transmembrane domain	201	220	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000153765

SMART Domains

Protein: ENSMUSP00000116155
Gene: ENSMUSG00000036339

Domain	Start	End	E-Value	Type
transmembrane domain	21	40	N/A	INTRINSIC
Pfam:DUF2723	48	111	8.3e-25	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000226422

Predicted Effect

probably null
Transcript: ENSMUST00000227440

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (66/66)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actn3	C	T	19: 4,871,616	V179I	probably benign	Het
Adra1b	A	G	11: 43,776,324	I362T	possibly damaging	Het
B3galnt1	T	C	3: 69,575,617	K104E	probably benign	Het
Bnc2	T	C	4: 84,555,864	Y15C	probably benign	Het
C2cd2l	C	A	9: 44,317,663	A124S	possibly damaging	Het
Camsap3	T	A	8: 3,604,116	F595L	probably damaging	Het
Cct2	A	T	10: 117,061,559	D93E	probably benign	Het
Cemip	A	T	7: 83,948,804		probably null	Het
Ciapin1	T	C	8: 94,823,710	T34A		Het
Dopey1	G	A	9: 86,515,378	V912I	probably damaging	Het
Eif3c	T	C	7: 126,552,323	T610A	possibly damaging	Het
Ephb3	A	G	16: 21,214,481	R106G	probably damaging	Het
Ephx3	T	A	17: 32,185,354		probably null	Het
Etv2	A	T	7: 30,635,030	S93T	probably benign	Het
Fbxo34	T	A	14: 47,530,384	D451E	probably benign	Het
Fmo9	A	G	1: 166,676,571	F141L	probably damaging	Het
Fryl	T	C	5: 73,108,478	K500R	possibly damaging	Het
Gfra3	T	C	18: 34,695,831	D170G	probably damaging	Het
Gm11639	A	T	11: 104,899,267	Q2832L	probably benign	Het
Gm11756	G	T	4: 73,917,571	L219M	probably benign	Het
Gm17783	A	T	16: 45,527,667	I169N	possibly damaging	Het
Gm4869	G	T	5: 140,471,645	A390S	probably benign	Het
Gnptg	T	C	17: 25,239,923	N34S	possibly damaging	Het
Golga5	T	A	12: 102,474,775		probably null	Het
Herc2	G	T	7: 56,085,080	L139F	probably benign	Het
Ikzf2	T	A	1: 69,539,081	N423I	probably benign	Het
Il31ra	A	T	13: 112,523,905	*717R	probably null	Het
Itga2	G	T	13: 114,877,691	Q234K	probably benign	Het
Kcnt1	A	T	2: 25,909,939		probably null	Het
Man2a2	T	C	7: 80,368,905	S69G	probably damaging	Het
Mccc1	A	G	3: 35,983,795	V294A	probably benign	Het
Mphosph8	T	A	14: 56,674,297	I259K	possibly damaging	Het
Mrpl15	A	T	1: 4,776,488	N288K	probably benign	Het
Msantd2	T	C	9: 37,489,669	W116R	probably damaging	Het
Mtmr10	T	C	7: 64,314,184		probably benign	Het
Mylk	A	T	16: 34,922,529	D1137V	probably damaging	Het
Nhsl1	A	G	10: 18,525,764	K879E	probably damaging	Het
Olfr1062	A	T	2: 86,423,189	C162*	probably null	Het
Pak6	A	G	2: 118,693,428	T355A	probably benign	Het
Patj	C	T	4: 98,411,057	R139C	probably damaging	Het
Pcdhb3	C	T	18: 37,301,452	A157V	probably damaging	Het
Phkg1	C	A	5: 129,866,961	D150Y	probably damaging	Het
Ppp2r5c	C	T	12: 110,465,889	S45L	probably benign	Het
Prl4a1	A	C	13: 28,018,573	T44P	probably benign	Het
Ptpro	G	A	6: 137,368,337	V114M	probably damaging	Het
Pwp1	A	G	10: 85,879,283	N211S	probably benign	Het
Ralgapb	A	G	2: 158,440,827	D504G	probably benign	Het
Rock2	A	T	12: 16,929,002	I98F	probably damaging	Het
Senp6	G	T	9: 80,132,965	V785L	probably damaging	Het
Simc1	A	G	13: 54,524,796	D319G	probably benign	Het
Tas2r105	A	T	6: 131,686,760	I235N	probably damaging	Het
Tas2r110	A	T	6: 132,868,704	M233L	possibly damaging	Het
Trank1	G	T	9: 111,373,074	V1590L	possibly damaging	Het
Tsc2	A	T	17: 24,623,594	M286K	possibly damaging	Het
Ttn	T	C	2: 76,867,452	T202A		Het
Ttyh1	A	G	7: 4,133,664	N424D	probably benign	Het
Usp24	T	A	4: 106,406,305		probably null	Het
Utp20	G	T	10: 88,749,342	P2620Q	probably benign	Het
Vmn1r45	A	G	6: 89,933,151	M279T	probably benign	Het
Vmn2r115	T	A	17: 23,359,602	I683K	probably benign	Het
Vmn2r34	T	C	7: 7,681,751	K481E	probably damaging	Het
Vmn2r54	T	A	7: 12,631,990	H339L	possibly damaging	Het
Vmn2r94	T	A	17: 18,257,549	N200I	possibly damaging	Het
Wdfy3	A	G	5: 101,836,208	Y3493H	probably damaging	Het
Wdr5b	A	G	16: 36,041,838	D109G	possibly damaging	Het
Zfp286	A	T	11: 62,783,670		probably null	Het

Other mutations in Tmem260

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00324:Tmem260	APN	14	48486879	missense	probably benign	0.27
IGL00338:Tmem260	APN	14	48477636	missense	probably damaging	0.97
IGL00508:Tmem260	APN	14	48509121	missense	probably damaging	0.99
IGL01308:Tmem260	APN	14	48511958	missense	probably damaging	1.00
IGL01548:Tmem260	APN	14	48480325	missense	possibly damaging	0.60
IGL01952:Tmem260	APN	14	48472476	missense	probably damaging	1.00
IGL03068:Tmem260	APN	14	48486914	splice site	probably benign
IGL03081:Tmem260	APN	14	48496293	missense	probably benign	0.14
R0131:Tmem260	UTSW	14	48483322	nonsense	probably null
R0131:Tmem260	UTSW	14	48483322	nonsense	probably null
R0132:Tmem260	UTSW	14	48483322	nonsense	probably null
R0149:Tmem260	UTSW	14	48452047	missense	possibly damaging	0.88
R0361:Tmem260	UTSW	14	48452047	missense	possibly damaging	0.88
R0396:Tmem260	UTSW	14	48486867	missense	possibly damaging	0.83
R0524:Tmem260	UTSW	14	48472478	missense	probably benign	0.00
R1734:Tmem260	UTSW	14	48509093	missense	probably benign	0.00
R2152:Tmem260	UTSW	14	48477609	missense	possibly damaging	0.62
R2474:Tmem260	UTSW	14	48496324	missense	probably null	0.90
R2928:Tmem260	UTSW	14	48486750	missense	probably damaging	1.00
R2999:Tmem260	UTSW	14	48484989	missense	possibly damaging	0.92
R3030:Tmem260	UTSW	14	48485001	missense	probably damaging	1.00
R4273:Tmem260	UTSW	14	48505304	missense	probably benign	0.11
R4276:Tmem260	UTSW	14	48477636	missense	probably damaging	0.97
R4277:Tmem260	UTSW	14	48477636	missense	probably damaging	0.97
R4278:Tmem260	UTSW	14	48477636	missense	probably damaging	0.97
R4791:Tmem260	UTSW	14	48511994	intron	probably benign
R4792:Tmem260	UTSW	14	48511994	intron	probably benign
R4810:Tmem260	UTSW	14	48472473	missense	probably damaging	0.99
R5189:Tmem260	UTSW	14	48509116	missense	probably benign	0.01
R5280:Tmem260	UTSW	14	48505259	missense	probably benign	0.02
R5289:Tmem260	UTSW	14	48486810	missense	possibly damaging	0.79
R5322:Tmem260	UTSW	14	48486849	missense	probably damaging	0.99
R5491:Tmem260	UTSW	14	48512170	splice site	probably null
R5593:Tmem260	UTSW	14	48474044	missense	probably benign	0.38
R5606:Tmem260	UTSW	14	48484980	missense	probably damaging	1.00
R5824:Tmem260	UTSW	14	48505328	missense	probably damaging	1.00
R5947:Tmem260	UTSW	14	48486801	missense	possibly damaging	0.93
R6587:Tmem260	UTSW	14	48496456	splice site	probably null
R7234:Tmem260	UTSW	14	48505329	nonsense	probably null
R7836:Tmem260	UTSW	14	48509062	missense	probably benign	0.04
R8795:Tmem260	UTSW	14	48451913	missense	probably damaging	1.00
R8895:Tmem260	UTSW	14	48500388	unclassified	probably benign
R9056:Tmem260	UTSW	14	48480317	missense	probably benign
R9096:Tmem260	UTSW	14	48520346	missense	unknown
R9384:Tmem260	UTSW	14	48486819	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CCATCTTGATGTTTCTAATGAGGG -3'
(R):5'- TGGCTCCTGGATACCACTTC -3'

Sequencing Primer
(F):5'- GTTCTTCCTCCACCCACAGAATG -3'
(R):5'- GGCTCCTATGATCATAGTGAACC -3'

Posted On

2019-09-23