Mutagenetix > Incidental Mutation

Incidental Mutation 'R7232:Tcf25'

574670

Institutional Source

Beutler Lab

Gene Symbol

Tcf25

Ensembl Gene

ENSMUSG00000001472

Gene Name

transcription factor 25 (basic helix-loop-helix)

Synonyms

Nulp1, 1810041K11Rik, D8Ertd325e, 1100001J13Rik

MMRRC Submission

045303-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7232 (G1)

Quality Score

204.009

Status

Validated

Chromosome

Chromosomal Location

124100492-124130574 bp(+) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

T to A at 124127800 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000148454 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057934] [ENSMUST00000108840] [ENSMUST00000127664] [ENSMUST00000211932] [ENSMUST00000212470] [ENSMUST00000212569] [ENSMUST00000212571] [ENSMUST00000212880]

AlphaFold

Q8R3L2

Predicted Effect

probably null
Transcript: ENSMUST00000057934

SMART Domains

Protein: ENSMUSP00000056485
Gene: ENSMUSG00000001472

Domain	Start	End	E-Value	Type
low complexity region	3	17	N/A	INTRINSIC
low complexity region	34	55	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
Pfam:Tcf25	248	588	4.6e-120	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000108840

SMART Domains

Protein: ENSMUSP00000104468
Gene: ENSMUSG00000001472

Domain	Start	End	E-Value	Type
low complexity region	3	17	N/A	INTRINSIC
low complexity region	34	55	N/A	INTRINSIC
low complexity region	124	136	N/A	INTRINSIC
Pfam:Tcf25	247	588	2.3e-113	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000127664

SMART Domains

Protein: ENSMUSP00000118564
Gene: ENSMUSG00000092329

Domain	Start	End	E-Value	Type
Pfam:Glycos_transf_2	104	287	7.4e-31	PFAM
Pfam:Glyco_transf_7C	261	331	4.9e-8	PFAM
RICIN	406	531	9.28e-27	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000211932

Predicted Effect

probably benign
Transcript: ENSMUST00000212470

Predicted Effect

probably null
Transcript: ENSMUST00000212569

Predicted Effect

probably benign
Transcript: ENSMUST00000212571

Predicted Effect

probably benign
Transcript: ENSMUST00000212880

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

99% (80/81)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] TCF25 is a member of the basic helix-loop-helix (bHLH) family of transcription factors that are important in embryonic development (Steen and Lindholm, 2008 [PubMed 18068114]).[supplied by OMIM, Sep 2008]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930433I11Rik	G	A	7: 40,642,603 (GRCm39)	G91S	probably damaging	Het
Adam21	T	G	12: 81,607,330 (GRCm39)	N144T	probably damaging	Het
Adgrg7	T	C	16: 56,597,515 (GRCm39)		probably null	Het
Angpt4	T	C	2: 151,771,460 (GRCm39)	S259P	possibly damaging	Het
Aqr	A	C	2: 113,936,363 (GRCm39)	L1320R	probably damaging	Het
Arhgef10	G	A	8: 14,990,323 (GRCm39)	G266D	probably benign	Het
Bop1	A	G	15: 76,337,546 (GRCm39)	V693A	probably damaging	Het
Cd86	CA	CAA	16: 36,426,917 (GRCm39)		probably null	Het
Cdc5l	A	G	17: 45,738,863 (GRCm39)		probably null	Het
Cfap46	C	A	7: 139,197,493 (GRCm39)	R2126L	unknown	Het
Chek2	T	C	5: 111,008,781 (GRCm39)	V304A	probably damaging	Het
Cntnap4	T	A	8: 113,391,731 (GRCm39)		probably null	Het
Ctps1	C	A	4: 120,405,321 (GRCm39)	G374C	probably damaging	Het
Defa27	A	T	8: 21,805,625 (GRCm39)	I22F	probably damaging	Het
Dnah14	T	C	1: 181,584,928 (GRCm39)	S3220P	probably damaging	Het
Dop1b	T	G	16: 93,557,373 (GRCm39)		probably null	Het
Dpysl5	G	A	5: 30,949,642 (GRCm39)	V471I	probably benign	Het
Duoxa1	T	A	2: 122,135,728 (GRCm39)	I124F	probably damaging	Het
Eif4enif1	A	G	11: 3,165,678 (GRCm39)	E85G	possibly damaging	Het
Eogt	T	A	6: 97,096,944 (GRCm39)	I355F	probably damaging	Het
Epha7	T	A	4: 28,951,279 (GRCm39)	V800E	probably damaging	Het
Evi5l	A	G	8: 4,255,906 (GRCm39)	Q633R	possibly damaging	Het
Fam170a	T	C	18: 50,414,728 (GRCm39)	Y125H	probably damaging	Het
Fbxo15	T	C	18: 84,980,747 (GRCm39)	Y241H	probably damaging	Het
Gbe1	T	C	16: 70,233,828 (GRCm39)	I235T	possibly damaging	Het
Gfra3	C	T	18: 34,844,234 (GRCm39)	R102Q	probably damaging	Het
Gjd4	C	T	18: 9,280,380 (GRCm39)	G233S	probably damaging	Het
Gm29106	C	T	1: 118,127,291 (GRCm39)	P328S	probably damaging	Het
Hgfac	G	T	5: 35,204,258 (GRCm39)	R507L	probably damaging	Het
Jakmip3	A	G	7: 138,609,355 (GRCm39)	K153R	probably benign	Het
Kcnc1	T	A	7: 46,077,383 (GRCm39)	V395E	probably damaging	Het
Krt6b	T	C	15: 101,586,577 (GRCm39)	D304G	probably damaging	Het
Ldha	T	C	7: 46,500,323 (GRCm39)	Y174H	probably benign	Het
Lgi4	G	A	7: 30,766,776 (GRCm39)	V268M	possibly damaging	Het
Lpar3	T	G	3: 145,947,061 (GRCm39)		probably null	Het
Lrp3	T	C	7: 34,905,477 (GRCm39)	D103G	probably damaging	Het
Marchf5	G	A	19: 37,194,713 (GRCm39)		probably null	Het
Muc5b	A	T	7: 141,419,866 (GRCm39)	H4115L	possibly damaging	Het
Myh13	G	A	11: 67,239,672 (GRCm39)	D741N	probably damaging	Het
Naglu	A	T	11: 100,967,252 (GRCm39)	I401F	probably damaging	Het
Ncam2	T	A	16: 81,309,759 (GRCm39)	N416K	probably damaging	Het
Ncan	A	G	8: 70,564,738 (GRCm39)	L292S	probably damaging	Het
Nkiras1	T	C	14: 18,276,732 (GRCm38)	V7A	probably damaging	Het
Nlrp4c	T	A	7: 6,068,708 (GRCm39)	L203*	probably null	Het
Nscme3l	A	T	19: 5,553,659 (GRCm39)	S41T	possibly damaging	Het
Onecut2	T	A	18: 64,474,633 (GRCm39)	W395R	probably damaging	Het
Or10a2	T	A	7: 106,673,062 (GRCm39)	I9K	probably benign	Het
Pak6	T	A	2: 118,524,003 (GRCm39)	V386E	probably damaging	Het
Pias2	A	G	18: 77,220,931 (GRCm39)	S396G	probably benign	Het
Plxna2	T	A	1: 194,394,568 (GRCm39)	L483H	probably damaging	Het
Prss36	G	T	7: 127,534,763 (GRCm39)	R484S	probably benign	Het
Ptpn21	A	T	12: 98,654,996 (GRCm39)	V657E	probably benign	Het
Rassf2	T	C	2: 131,838,332 (GRCm39)	E318G	probably damaging	Het
Rp1	A	C	1: 4,298,824 (GRCm39)	S623A	unknown	Het
Runx2	G	A	17: 45,125,079 (GRCm39)	P80L	probably damaging	Het
Scn11a	T	A	9: 119,588,982 (GRCm39)	E1308V	probably damaging	Het
Serpina3c	A	T	12: 104,115,771 (GRCm39)	S258T	possibly damaging	Het
Slit3	A	G	11: 35,501,516 (GRCm39)	T417A	possibly damaging	Het
Sostdc1	C	A	12: 36,367,310 (GRCm39)	A162E	possibly damaging	Het
Sox13	A	T	1: 133,312,129 (GRCm39)		probably null	Het
Sox8	G	T	17: 25,786,514 (GRCm39)	S396R	probably benign	Het
Sult2a3	A	T	7: 13,816,685 (GRCm39)	F164L	possibly damaging	Het
Susd2	T	C	10: 75,475,685 (GRCm39)	Y438C	probably damaging	Het
Tas2r106	T	G	6: 131,655,810 (GRCm39)	T14P	probably damaging	Het
Tenm3	G	A	8: 48,688,970 (GRCm39)	R2206W	probably damaging	Het
Tep1	A	G	14: 51,081,789 (GRCm39)	L471P	unknown	Het
Ticrr	A	T	7: 79,343,490 (GRCm39)	K1118N	probably damaging	Het
Tlr5	G	A	1: 182,801,064 (GRCm39)	E123K	probably benign	Het
Ttn	ATATCTCTCCAGAGCCTCCCCTGGAGGAGTGGAGTATCTCTCCAGAGCCTCCCCTGGAGGAGTGGAGTATCTCTCCAGAGCCTCCCCTG	ATATCTCTCCAGAGCCTCCCCTGGAGGAGTGGAGTATCTCTCCAGAGCCTCCCCTG	2: 76,746,150 (GRCm39)		probably benign	Het
U2surp	A	G	9: 95,375,770 (GRCm39)	V141A	probably benign	Het
Unc79	T	A	12: 103,100,734 (GRCm39)	S2050T	possibly damaging	Het
Usp13	A	G	3: 32,920,020 (GRCm39)	D235G	probably benign	Het
Vcam1	T	C	3: 115,919,628 (GRCm39)	T213A	possibly damaging	Het
Vmn2r60	T	A	7: 41,786,166 (GRCm39)	I323N	possibly damaging	Het
Vps13b	A	G	15: 35,877,703 (GRCm39)	I2892M	probably damaging	Het
Wnt5a	T	A	14: 28,240,329 (GRCm39)	S160T	probably benign	Het
Zar1	G	A	5: 72,738,294 (GRCm39)	P36L	possibly damaging	Het
Zfp773	A	T	7: 7,135,984 (GRCm39)	M204K	probably benign	Het
Zhx1	T	C	15: 57,916,465 (GRCm39)	T594A	probably benign	Het

Other mutations in Tcf25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01790:Tcf25	APN	8	124,119,975 (GRCm39)	missense	possibly damaging	0.87
IGL02638:Tcf25	APN	8	124,126,031 (GRCm39)	missense	probably damaging	1.00
IGL03112:Tcf25	APN	8	124,109,258 (GRCm39)	splice site	probably benign
R0492:Tcf25	UTSW	8	124,108,203 (GRCm39)	missense	probably benign	0.00
R1081:Tcf25	UTSW	8	124,108,212 (GRCm39)	missense	probably benign	0.00
R1543:Tcf25	UTSW	8	124,115,326 (GRCm39)	missense	probably benign	0.01
R1634:Tcf25	UTSW	8	124,123,830 (GRCm39)	missense	possibly damaging	0.68
R1662:Tcf25	UTSW	8	124,108,289 (GRCm39)	missense	probably benign	0.00
R2253:Tcf25	UTSW	8	124,100,772 (GRCm39)	missense	probably benign	0.21
R4326:Tcf25	UTSW	8	124,127,882 (GRCm39)	nonsense	probably null
R4327:Tcf25	UTSW	8	124,127,882 (GRCm39)	nonsense	probably null
R4667:Tcf25	UTSW	8	124,123,764 (GRCm39)	missense	possibly damaging	0.89
R4977:Tcf25	UTSW	8	124,115,374 (GRCm39)	missense	probably benign	0.03
R5248:Tcf25	UTSW	8	124,100,678 (GRCm39)	missense	probably damaging	1.00
R5249:Tcf25	UTSW	8	124,115,372 (GRCm39)	missense	probably damaging	1.00
R5759:Tcf25	UTSW	8	124,108,196 (GRCm39)	missense	probably benign	0.00
R5806:Tcf25	UTSW	8	124,108,243 (GRCm39)	missense	probably benign	0.09
R5813:Tcf25	UTSW	8	124,122,354 (GRCm39)	splice site	probably null
R5905:Tcf25	UTSW	8	124,108,176 (GRCm39)	missense	possibly damaging	0.78
R6028:Tcf25	UTSW	8	124,108,176 (GRCm39)	missense	possibly damaging	0.78
R6114:Tcf25	UTSW	8	124,111,114 (GRCm39)	missense	probably damaging	1.00
R6349:Tcf25	UTSW	8	124,118,332 (GRCm39)	missense	probably damaging	1.00
R6904:Tcf25	UTSW	8	124,127,437 (GRCm39)	critical splice donor site	probably null
R7287:Tcf25	UTSW	8	124,100,711 (GRCm39)	missense	possibly damaging	0.74
R9062:Tcf25	UTSW	8	124,116,448 (GRCm39)	missense
R9135:Tcf25	UTSW	8	124,108,182 (GRCm39)	missense	probably benign	0.00
R9396:Tcf25	UTSW	8	124,127,831 (GRCm39)	missense	probably benign	0.00
RF007:Tcf25	UTSW	8	124,122,369 (GRCm39)	missense	probably benign	0.03
Z1176:Tcf25	UTSW	8	124,100,645 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TCAGCATCATGGTAGGTTGGC -3'
(R):5'- TCGGAACTGGTTAACTCCAAC -3'

Sequencing Primer
(F):5'- CATCATGGTAGGTTGGCTTTGGC -3'
(R):5'- AGCATCTGGACAGTTCAGTC -3'

Posted On

2019-10-02