Mutagenetix > Incidental Mutation

Incidental Mutation 'R7407:Vmn2r28'

574786

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r28

Ensembl Gene

ENSMUSG00000066820

Gene Name

vomeronasal 2, receptor 28

Synonyms

EG665255

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R7407 (G1)

Quality Score

190.009

Status

Not validated

Chromosome

Chromosomal Location

5479531-5493967 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 5481309 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 631 (S631P)

Ref Sequence

ENSEMBL: ENSMUSP00000083477 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086297]

AlphaFold

L7N203

Predicted Effect

probably damaging
Transcript: ENSMUST00000086297
AA Change: S631P

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000083477
Gene: ENSMUSG00000066820
AA Change: S631P

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	1.7e-26	PFAM
Pfam:NCD3G	512	565	9.8e-21	PFAM
Pfam:7tm_3	598	833	2.8e-56	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933405O20Rik	A	G	7: 50,599,878	N220S	probably damaging	Het
Abcb8	T	C	5: 24,400,676	V186A	probably benign	Het
Actbl2	A	T	13: 111,256,218	E362D	probably damaging	Het
Adamts17	C	A	7: 67,047,556	Y28*	probably null	Het
Agtr1b	T	C	3: 20,315,731	D237G	possibly damaging	Het
Amer2	A	G	14: 60,378,842	D162G	probably damaging	Het
Ankub1	T	C	3: 57,665,203	E366G	probably benign	Het
Ap5z1	A	G	5: 142,466,575	I88V	probably benign	Het
BC053393	A	G	11: 46,577,390	Y97C	probably damaging	Het
Ccdc186	T	C	19: 56,813,385	N100S	probably benign	Het
Cldn19	A	G	4: 119,255,685	D38G	probably damaging	Het
Crat	C	T	2: 30,404,565	R497Q	probably benign	Het
Deaf1	G	A	7: 141,297,579	A545V	possibly damaging	Het
Dicer1	G	T	12: 104,722,351	Y322*	probably null	Het
Dnajb7	G	T	15: 81,407,626	T170K	possibly damaging	Het
Flrt2	A	T	12: 95,779,300	E137D	probably damaging	Het
Galnt12	T	C	4: 47,120,362	F482L	probably damaging	Het
Gm14025	A	G	2: 129,038,809	I399T		Het
Gm32742	A	G	9: 51,156,674	V336A	probably damaging	Het
Gpatch8	G	A	11: 102,479,830	R961W	unknown	Het
Hcn4	A	G	9: 58,859,370	E738G	unknown	Het
Kdelr3	A	G	15: 79,524,838	Y76C	probably damaging	Het
Krt77	T	C	15: 101,860,095	S494G	unknown	Het
Letmd1	G	T	15: 100,469,238	A39S	probably benign	Het
Lrrc7	G	A	3: 158,135,241	R1387W	probably damaging	Het
Meltf	T	C	16: 31,894,735	Y599H	probably damaging	Het
Mybpc1	T	A	10: 88,549,347	I477L	probably damaging	Het
Nf1	A	G	11: 79,448,143	D1174G	probably damaging	Het
Olfr720	T	A	14: 14,175,402	I227L	probably benign	Het
Olfr907	T	A	9: 38,499,504	Y278*	probably null	Het
Palld	G	T	8: 61,515,941	S1283*	probably null	Het
Pcmtd2	T	C	2: 181,846,605	V183A	possibly damaging	Het
Pcsk5	T	A	19: 17,675,516	I269F	probably damaging	Het
Pkd1	T	C	17: 24,594,594	L4036P	probably damaging	Het
Pkhd1l1	A	G	15: 44,595,011	N4151S	possibly damaging	Het
Rcor3	C	T	1: 192,101,672	S422N	probably benign	Het
Rhag	A	G	17: 40,831,334	I223V	possibly damaging	Het
Ssbp4	A	G	8: 70,599,022	Y231H	probably damaging	Het
Syce1l	C	T	8: 113,655,138	Q237*	probably null	Het
Tenm4	T	C	7: 96,773,987	V663A	possibly damaging	Het
Trim6	T	C	7: 104,225,901	I115T	probably damaging	Het
Vmn1r25	T	A	6: 57,979,059	T82S	possibly damaging	Het
Xpo1	T	A	11: 23,285,823	V637E	probably damaging	Het
Xpo6	A	T	7: 126,171,052	M62K	probably damaging	Het
Ypel5	A	G	17: 72,846,379	N26S	possibly damaging	Het
Zbtb24	C	A	10: 41,464,779	Q624K	possibly damaging	Het
Zfp629	T	C	7: 127,610,243	D798G	probably benign	Het
Zfp687	C	T	3: 95,007,530	R1220H	probably damaging	Het

Other mutations in Vmn2r28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Vmn2r28	APN	7	5488069	missense	probably benign	0.12
IGL01061:Vmn2r28	APN	7	5488184	missense	probably damaging	1.00
IGL01160:Vmn2r28	APN	7	5486478	missense	probably damaging	0.99
IGL01335:Vmn2r28	APN	7	5481088	missense	possibly damaging	0.67
IGL01532:Vmn2r28	APN	7	5486464	missense	probably benign	0.16
IGL01791:Vmn2r28	APN	7	5488162	missense	probably benign	0.00
IGL01875:Vmn2r28	APN	7	5481303	missense	probably benign	0.30
IGL02161:Vmn2r28	APN	7	5488124	missense	possibly damaging	0.88
IGL02499:Vmn2r28	APN	7	5490569	missense	probably damaging	0.98
IGL02858:Vmn2r28	APN	7	5481004	missense	probably damaging	0.99
IGL03061:Vmn2r28	APN	7	5484016	missense	probably damaging	0.98
G1patch:Vmn2r28	UTSW	7	5488409	missense	probably benign	0.13
R0288:Vmn2r28	UTSW	7	5488021	missense	probably damaging	1.00
R0361:Vmn2r28	UTSW	7	5493716	missense	probably benign	0.00
R0396:Vmn2r28	UTSW	7	5488514	missense	probably benign	0.05
R0480:Vmn2r28	UTSW	7	5490457	missense	probably benign	0.00
R0485:Vmn2r28	UTSW	7	5488690	missense	probably damaging	1.00
R0837:Vmn2r28	UTSW	7	5488027	missense	probably damaging	0.99
R1282:Vmn2r28	UTSW	7	5481302	missense	probably damaging	0.99
R1296:Vmn2r28	UTSW	7	5481545	missense	possibly damaging	0.81
R1829:Vmn2r28	UTSW	7	5493811	missense	probably benign
R1853:Vmn2r28	UTSW	7	5481247	nonsense	probably null
R1869:Vmn2r28	UTSW	7	5486346	missense	probably benign	0.00
R1887:Vmn2r28	UTSW	7	5488289	missense	possibly damaging	0.90
R1961:Vmn2r28	UTSW	7	5481071	missense	possibly damaging	0.85
R1998:Vmn2r28	UTSW	7	5488314	missense	possibly damaging	0.87
R2392:Vmn2r28	UTSW	7	5484131	missense	probably damaging	0.98
R2432:Vmn2r28	UTSW	7	5488702	missense	probably damaging	0.99
R3055:Vmn2r28	UTSW	7	5481392	missense	probably damaging	0.98
R3753:Vmn2r28	UTSW	7	5488027	missense	probably damaging	0.99
R3877:Vmn2r28	UTSW	7	5488358	missense	probably damaging	1.00
R4307:Vmn2r28	UTSW	7	5490708	missense	probably damaging	0.99
R5023:Vmn2r28	UTSW	7	5486464	missense	probably benign	0.16
R5057:Vmn2r28	UTSW	7	5486464	missense	probably benign	0.16
R5083:Vmn2r28	UTSW	7	5480672	missense	possibly damaging	0.77
R5427:Vmn2r28	UTSW	7	5486377	missense	probably damaging	0.99
R5472:Vmn2r28	UTSW	7	5487944	critical splice donor site	probably null
R5511:Vmn2r28	UTSW	7	5484012	missense	possibly damaging	0.61
R5731:Vmn2r28	UTSW	7	5488669	missense	probably benign	0.41
R6091:Vmn2r28	UTSW	7	5493791	missense	possibly damaging	0.88
R6179:Vmn2r28	UTSW	7	5488004	nonsense	probably null
R6276:Vmn2r28	UTSW	7	5490731	missense	probably benign	0.01
R6441:Vmn2r28	UTSW	7	5488475	missense	probably benign	0.00
R6463:Vmn2r28	UTSW	7	5486436	missense	probably benign	0.07
R6528:Vmn2r28	UTSW	7	5490685	missense	probably benign	0.12
R6725:Vmn2r28	UTSW	7	5488409	missense	probably benign	0.13
R6760:Vmn2r28	UTSW	7	5481230	missense	probably damaging	0.97
R6849:Vmn2r28	UTSW	7	5480807	missense	probably damaging	1.00
R7110:Vmn2r28	UTSW	7	5490734	missense	probably benign	0.06
R7146:Vmn2r28	UTSW	7	5481496	missense	probably benign	0.05
R7563:Vmn2r28	UTSW	7	5488201	missense	probably benign	0.00
R7611:Vmn2r28	UTSW	7	5481256	missense	probably benign	0.02
R7808:Vmn2r28	UTSW	7	5493679	missense	probably damaging	0.98
R7862:Vmn2r28	UTSW	7	5490614	missense	probably benign	0.00
R7916:Vmn2r28	UTSW	7	5480819	missense	probably damaging	1.00
R8183:Vmn2r28	UTSW	7	5488148	missense	probably damaging	1.00
R8334:Vmn2r28	UTSW	7	5484060	missense	probably damaging	1.00
R8519:Vmn2r28	UTSW	7	5486348	missense	probably benign	0.31
R8989:Vmn2r28	UTSW	7	5490474	missense	probably benign	0.00
R9066:Vmn2r28	UTSW	7	5488597	missense	probably damaging	1.00
R9422:Vmn2r28	UTSW	7	5480748	missense	probably damaging	1.00
R9469:Vmn2r28	UTSW	7	5484119	missense	probably damaging	0.99
R9608:Vmn2r28	UTSW	7	5488221	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- TAACGTTCTTCCTGGGCCTG -3'
(R):5'- GTGTGAATTGTCCAGAATACCAGTATG -3'

Sequencing Primer
(F):5'- GGCCTGTGAATTTGAAAGCC -3'
(R):5'- CAAATGCATTCAGAAAGCTGTG -3'

Posted On

2019-10-07