Mutagenetix > Incidental Mutation

Incidental Mutation 'R7407:Tenm4'

574789

Institutional Source

Beutler Lab

Gene Symbol

Tenm4

Ensembl Gene

ENSMUSG00000048078

Gene Name

teneurin transmembrane protein 4

Synonyms

l7Rn3, Doc4, Ten-m4, ELM2, l(7)-3Rn, Odz4

MMRRC Submission

045488-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7407 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

95820453-96560300 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 96423194 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 663 (V663A)

Ref Sequence

ENSEMBL: ENSMUSP00000102783 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000107162] [ENSMUST00000107165] [ENSMUST00000107166]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107162
AA Change: V671A

PolyPhen 2 Score 0.821 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000102780
Gene: ENSMUSG00000048078
AA Change: V671A

Domain	Start	End	E-Value	Type
Pfam:Ten_N	10	410	5.6e-195	PFAM
transmembrane domain	411	433	N/A	INTRINSIC
EGF_like	637	665	3.43e1	SMART
EGF	668	696	2.29e1	SMART
EGF	701	730	1.88e-1	SMART
EGF	733	762	1.13e1	SMART
EGF	767	797	2.39e1	SMART
EGF	800	828	4.32e-1	SMART
EGF	831	859	6.02e0	SMART
EGF	862	894	9.93e-1	SMART
low complexity region	900	914	N/A	INTRINSIC
Pfam:RHS_repeat	2327	2380	5.5e-7	PFAM
Pfam:Tox-GHH	2740	2818	5.2e-34	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107165
AA Change: V663A

PolyPhen 2 Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000102783
Gene: ENSMUSG00000048078
AA Change: V663A

Domain	Start	End	E-Value	Type
Pfam:Ten_N	36	402	1.1e-171	PFAM
transmembrane domain	403	425	N/A	INTRINSIC
EGF_like	629	657	3.43e1	SMART
EGF	660	688	2.29e1	SMART
EGF	693	722	1.88e-1	SMART
EGF	725	754	1.13e1	SMART
EGF	759	789	2.39e1	SMART
EGF	792	820	4.32e-1	SMART
EGF	823	851	6.02e0	SMART
EGF	863	895	9.93e-1	SMART
low complexity region	901	915	N/A	INTRINSIC
Pfam:RHS_repeat	2335	2368	1.6e-7	PFAM
Pfam:Tox-GHH	2749	2826	1.8e-32	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107166
AA Change: V626A

PolyPhen 2 Score 0.909 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000102784
Gene: ENSMUSG00000048078
AA Change: V626A

Domain	Start	End	E-Value	Type
Pfam:Ten_N	35	193	1.4e-83	PFAM
Pfam:Ten_N	187	365	5e-78	PFAM
transmembrane domain	366	388	N/A	INTRINSIC
EGF_like	592	620	3.43e1	SMART
EGF	623	651	2.29e1	SMART
EGF	656	685	1.88e-1	SMART
EGF	688	717	1.13e1	SMART
EGF	722	752	2.39e1	SMART
EGF	755	783	4.32e-1	SMART
EGF	786	814	6.02e0	SMART
EGF	826	858	9.93e-1	SMART
low complexity region	864	878	N/A	INTRINSIC
Pfam:RHS_repeat	2298	2351	3.8e-7	PFAM
Pfam:Tox-GHH	2711	2789	3.9e-34	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000140140

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene plays a role in establishing proper neuronal connectivity during development. Defects in this gene have been associated with hereditary essential tremor-5. [provided by RefSeq, Oct 2016]
PHENOTYPE: Various ENU-induced alleles cause prenatal lethality associated with impaired mesoderm development and lead to pleiotropic phenotypes. The most severe alleles cause failure of gastrulation and somitogenesis while the least severe one allows survival to adulthood with runting of variable penetrance. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933405O20Rik	A	G	7: 50,249,626 (GRCm39)	N220S	probably damaging	Het
Abcb8	T	C	5: 24,605,674 (GRCm39)	V186A	probably benign	Het
Actbl2	A	T	13: 111,392,752 (GRCm39)	E362D	probably damaging	Het
Adamts17	C	A	7: 66,697,304 (GRCm39)	Y28*	probably null	Het
Agtr1b	T	C	3: 20,369,895 (GRCm39)	D237G	possibly damaging	Het
Amer2	A	G	14: 60,616,291 (GRCm39)	D162G	probably damaging	Het
Ankub1	T	C	3: 57,572,624 (GRCm39)	E366G	probably benign	Het
Ap5z1	A	G	5: 142,452,330 (GRCm39)	I88V	probably benign	Het
Ccdc186	T	C	19: 56,801,817 (GRCm39)	N100S	probably benign	Het
Cldn19	A	G	4: 119,112,882 (GRCm39)	D38G	probably damaging	Het
Crat	C	T	2: 30,294,577 (GRCm39)	R497Q	probably benign	Het
Deaf1	G	A	7: 140,877,492 (GRCm39)	A545V	possibly damaging	Het
Dicer1	G	T	12: 104,688,610 (GRCm39)	Y322*	probably null	Het
Dnajb7	G	T	15: 81,291,827 (GRCm39)	T170K	possibly damaging	Het
Flrt2	A	T	12: 95,746,074 (GRCm39)	E137D	probably damaging	Het
Galnt12	T	C	4: 47,120,362 (GRCm39)	F482L	probably damaging	Het
Gm32742	A	G	9: 51,067,974 (GRCm39)	V336A	probably damaging	Het
Gpatch8	G	A	11: 102,370,656 (GRCm39)	R961W	unknown	Het
Hcn4	A	G	9: 58,766,653 (GRCm39)	E738G	unknown	Het
Kdelr3	A	G	15: 79,409,039 (GRCm39)	Y76C	probably damaging	Het
Krt77	T	C	15: 101,768,530 (GRCm39)	S494G	unknown	Het
Letmd1	G	T	15: 100,367,119 (GRCm39)	A39S	probably benign	Het
Lrrc7	G	A	3: 157,840,878 (GRCm39)	R1387W	probably damaging	Het
Meltf	T	C	16: 31,713,553 (GRCm39)	Y599H	probably damaging	Het
Mybpc1	T	A	10: 88,385,209 (GRCm39)	I477L	probably damaging	Het
Nf1	A	G	11: 79,338,969 (GRCm39)	D1174G	probably damaging	Het
Or2t6	T	A	14: 14,175,402 (GRCm38)	I227L	probably benign	Het
Or8b44	T	A	9: 38,410,800 (GRCm39)	Y278*	probably null	Het
Palld	G	T	8: 61,968,975 (GRCm39)	S1283*	probably null	Het
Pcmtd2	T	C	2: 181,488,398 (GRCm39)	V183A	possibly damaging	Het
Pcsk5	T	A	19: 17,652,880 (GRCm39)	I269F	probably damaging	Het
Pkd1	T	C	17: 24,813,568 (GRCm39)	L4036P	probably damaging	Het
Pkhd1l1	A	G	15: 44,458,407 (GRCm39)	N4151S	possibly damaging	Het
Rcor3	C	T	1: 191,785,972 (GRCm39)	S422N	probably benign	Het
Rhag	A	G	17: 41,142,225 (GRCm39)	I223V	possibly damaging	Het
Ssbp4	A	G	8: 71,051,672 (GRCm39)	Y231H	probably damaging	Het
Syce1l	C	T	8: 114,381,770 (GRCm39)	Q237*	probably null	Het
Timd6	A	G	11: 46,468,217 (GRCm39)	Y97C	probably damaging	Het
Trim6	T	C	7: 103,875,108 (GRCm39)	I115T	probably damaging	Het
Vinac1	A	G	2: 128,880,729 (GRCm39)	I399T		Het
Vmn1r25	T	A	6: 57,956,044 (GRCm39)	T82S	possibly damaging	Het
Vmn2r28	A	G	7: 5,484,308 (GRCm39)	S631P	probably damaging	Het
Xpo1	T	A	11: 23,235,823 (GRCm39)	V637E	probably damaging	Het
Xpo6	A	T	7: 125,770,224 (GRCm39)	M62K	probably damaging	Het
Ypel5	A	G	17: 73,153,374 (GRCm39)	N26S	possibly damaging	Het
Zbtb24	C	A	10: 41,340,775 (GRCm39)	Q624K	possibly damaging	Het
Zfp629	T	C	7: 127,209,415 (GRCm39)	D798G	probably benign	Het
Zfp687	C	T	3: 94,914,841 (GRCm39)	R1220H	probably damaging	Het

Other mutations in Tenm4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00228:Tenm4	APN	7	96,517,216 (GRCm39)	missense	probably benign	0.00
IGL00468:Tenm4	APN	7	96,523,679 (GRCm39)	missense	probably damaging	0.98
IGL00519:Tenm4	APN	7	96,454,345 (GRCm39)	splice site	probably benign
IGL00979:Tenm4	APN	7	96,378,598 (GRCm39)	missense	probably damaging	0.96
IGL01401:Tenm4	APN	7	96,523,474 (GRCm39)	missense	probably damaging	1.00
IGL01459:Tenm4	APN	7	96,378,592 (GRCm39)	missense	probably damaging	1.00
IGL01519:Tenm4	APN	7	96,544,384 (GRCm39)	missense	probably damaging	1.00
IGL01545:Tenm4	APN	7	96,523,510 (GRCm39)	missense	probably benign	0.00
IGL01579:Tenm4	APN	7	96,512,709 (GRCm39)	missense	probably benign	0.00
IGL01587:Tenm4	APN	7	96,512,709 (GRCm39)	missense	probably benign	0.00
IGL01625:Tenm4	APN	7	96,534,565 (GRCm39)	missense	probably damaging	1.00
IGL01655:Tenm4	APN	7	96,202,931 (GRCm39)	missense	probably damaging	1.00
IGL01683:Tenm4	APN	7	96,534,611 (GRCm39)	missense	possibly damaging	0.84
IGL01728:Tenm4	APN	7	96,545,271 (GRCm39)	missense	probably damaging	1.00
IGL01732:Tenm4	APN	7	96,544,716 (GRCm39)	missense	probably damaging	1.00
IGL01924:Tenm4	APN	7	96,544,419 (GRCm39)	missense	probably damaging	1.00
IGL01966:Tenm4	APN	7	96,202,757 (GRCm39)	missense	probably damaging	1.00
IGL02177:Tenm4	APN	7	96,544,869 (GRCm39)	missense	probably benign	0.40
IGL02207:Tenm4	APN	7	96,523,323 (GRCm39)	missense	possibly damaging	0.85
IGL02269:Tenm4	APN	7	96,473,029 (GRCm39)	missense	probably damaging	1.00
IGL02274:Tenm4	APN	7	96,503,941 (GRCm39)	missense	probably damaging	1.00
IGL02375:Tenm4	APN	7	96,353,344 (GRCm39)	missense	possibly damaging	0.52
IGL02415:Tenm4	APN	7	96,523,281 (GRCm39)	missense	probably damaging	0.98
IGL02472:Tenm4	APN	7	96,423,383 (GRCm39)	unclassified	probably benign
IGL02656:Tenm4	APN	7	96,534,640 (GRCm39)	missense	probably damaging	1.00
IGL02678:Tenm4	APN	7	96,545,426 (GRCm39)	missense	probably damaging	1.00
IGL02829:Tenm4	APN	7	96,544,205 (GRCm39)	nonsense	probably null
IGL02863:Tenm4	APN	7	96,522,913 (GRCm39)	missense	probably damaging	1.00
IGL03145:Tenm4	APN	7	96,492,175 (GRCm39)	missense	probably damaging	0.98
IGL03153:Tenm4	APN	7	96,522,969 (GRCm39)	missense	probably damaging	1.00
principium	UTSW	7	96,446,688 (GRCm39)	missense	probably damaging	0.98
toccata	UTSW	7	96,552,196 (GRCm39)	critical splice donor site	probably null
P0026:Tenm4	UTSW	7	96,523,734 (GRCm39)	missense	probably damaging	1.00
R0097:Tenm4	UTSW	7	96,542,133 (GRCm39)	missense	probably damaging	1.00
R0097:Tenm4	UTSW	7	96,542,133 (GRCm39)	missense	probably damaging	1.00
R0140:Tenm4	UTSW	7	96,545,259 (GRCm39)	missense	possibly damaging	0.78
R0164:Tenm4	UTSW	7	96,378,547 (GRCm39)	splice site	probably benign
R0277:Tenm4	UTSW	7	96,344,157 (GRCm39)	missense	possibly damaging	0.54
R0323:Tenm4	UTSW	7	96,344,157 (GRCm39)	missense	possibly damaging	0.54
R0362:Tenm4	UTSW	7	96,421,242 (GRCm39)	nonsense	probably null
R0381:Tenm4	UTSW	7	96,555,088 (GRCm39)	missense	probably damaging	1.00
R0420:Tenm4	UTSW	7	96,522,973 (GRCm39)	missense	possibly damaging	0.85
R0426:Tenm4	UTSW	7	96,427,058 (GRCm39)	missense	probably damaging	1.00
R0513:Tenm4	UTSW	7	96,544,830 (GRCm39)	missense	probably benign	0.35
R0624:Tenm4	UTSW	7	96,423,227 (GRCm39)	missense	probably damaging	1.00
R0837:Tenm4	UTSW	7	96,545,482 (GRCm39)	splice site	probably benign
R1037:Tenm4	UTSW	7	96,446,688 (GRCm39)	missense	probably damaging	0.98
R1172:Tenm4	UTSW	7	96,497,251 (GRCm39)	missense	probably damaging	1.00
R1422:Tenm4	UTSW	7	96,199,258 (GRCm39)	missense	probably damaging	0.99
R1427:Tenm4	UTSW	7	96,492,255 (GRCm39)	missense	probably benign	0.42
R1462:Tenm4	UTSW	7	96,353,360 (GRCm39)	missense	probably damaging	1.00
R1462:Tenm4	UTSW	7	96,353,360 (GRCm39)	missense	probably damaging	1.00
R1597:Tenm4	UTSW	7	96,552,196 (GRCm39)	critical splice donor site	probably null
R1701:Tenm4	UTSW	7	96,552,096 (GRCm39)	missense	probably damaging	1.00
R1707:Tenm4	UTSW	7	96,537,892 (GRCm39)	missense	probably damaging	1.00
R1809:Tenm4	UTSW	7	96,522,987 (GRCm39)	missense	probably benign	0.17
R1812:Tenm4	UTSW	7	96,545,147 (GRCm39)	missense	probably damaging	1.00
R1895:Tenm4	UTSW	7	96,385,015 (GRCm39)	missense	probably damaging	1.00
R1933:Tenm4	UTSW	7	96,544,533 (GRCm39)	missense	probably damaging	1.00
R1946:Tenm4	UTSW	7	96,385,015 (GRCm39)	missense	probably damaging	1.00
R2108:Tenm4	UTSW	7	96,555,497 (GRCm39)	missense	probably damaging	1.00
R2151:Tenm4	UTSW	7	96,552,054 (GRCm39)	missense	probably damaging	1.00
R2247:Tenm4	UTSW	7	96,555,216 (GRCm39)	missense	probably benign	0.03
R2329:Tenm4	UTSW	7	96,545,069 (GRCm39)	missense	probably benign	0.00
R2893:Tenm4	UTSW	7	96,544,197 (GRCm39)	missense	probably damaging	1.00
R2990:Tenm4	UTSW	7	96,542,332 (GRCm39)	splice site	probably null
R3409:Tenm4	UTSW	7	96,544,367 (GRCm39)	missense	probably damaging	1.00
R3410:Tenm4	UTSW	7	96,501,737 (GRCm39)	missense	probably damaging	0.99
R3411:Tenm4	UTSW	7	96,501,737 (GRCm39)	missense	probably damaging	0.99
R3440:Tenm4	UTSW	7	96,202,723 (GRCm39)	missense	probably benign	0.00
R3441:Tenm4	UTSW	7	96,202,723 (GRCm39)	missense	probably benign	0.00
R3719:Tenm4	UTSW	7	96,512,770 (GRCm39)	missense	possibly damaging	0.92
R3772:Tenm4	UTSW	7	96,344,087 (GRCm39)	missense	probably damaging	1.00
R3773:Tenm4	UTSW	7	96,344,087 (GRCm39)	missense	probably damaging	1.00
R4093:Tenm4	UTSW	7	96,544,979 (GRCm39)	missense	probably damaging	1.00
R4439:Tenm4	UTSW	7	96,545,022 (GRCm39)	missense	probably benign	0.01
R4441:Tenm4	UTSW	7	96,545,022 (GRCm39)	missense	probably benign	0.01
R4510:Tenm4	UTSW	7	96,544,070 (GRCm39)	missense	probably benign
R4511:Tenm4	UTSW	7	96,544,070 (GRCm39)	missense	probably benign
R4543:Tenm4	UTSW	7	96,545,022 (GRCm39)	missense	probably benign	0.01
R4645:Tenm4	UTSW	7	96,544,949 (GRCm39)	missense	probably damaging	1.00
R4701:Tenm4	UTSW	7	96,544,556 (GRCm39)	missense	probably damaging	1.00
R4707:Tenm4	UTSW	7	96,423,253 (GRCm39)	missense	probably damaging	0.99
R4714:Tenm4	UTSW	7	96,544,131 (GRCm39)	missense	probably damaging	1.00
R4742:Tenm4	UTSW	7	96,446,691 (GRCm39)	missense	probably damaging	0.99
R4784:Tenm4	UTSW	7	96,423,253 (GRCm39)	missense	probably damaging	0.99
R4785:Tenm4	UTSW	7	96,423,253 (GRCm39)	missense	probably damaging	0.99
R4801:Tenm4	UTSW	7	96,555,452 (GRCm39)	missense	probably damaging	0.97
R4802:Tenm4	UTSW	7	96,555,452 (GRCm39)	missense	probably damaging	0.97
R4880:Tenm4	UTSW	7	96,555,025 (GRCm39)	splice site	probably null
R5036:Tenm4	UTSW	7	96,501,768 (GRCm39)	missense	probably damaging	1.00
R5036:Tenm4	UTSW	7	96,343,997 (GRCm39)	missense	probably damaging	1.00
R5050:Tenm4	UTSW	7	96,544,995 (GRCm39)	missense	probably damaging	1.00
R5103:Tenm4	UTSW	7	96,492,164 (GRCm39)	missense	probably damaging	1.00
R5106:Tenm4	UTSW	7	96,492,356 (GRCm39)	missense	probably damaging	0.99
R5118:Tenm4	UTSW	7	96,542,293 (GRCm39)	missense	probably damaging	1.00
R5272:Tenm4	UTSW	7	96,523,410 (GRCm39)	missense	probably damaging	0.98
R5282:Tenm4	UTSW	7	96,486,538 (GRCm39)	missense	possibly damaging	0.90
R5403:Tenm4	UTSW	7	96,538,034 (GRCm39)	missense	probably damaging	1.00
R5404:Tenm4	UTSW	7	96,543,887 (GRCm39)	missense	probably damaging	1.00
R5567:Tenm4	UTSW	7	96,545,416 (GRCm39)	nonsense	probably null
R5590:Tenm4	UTSW	7	96,446,608 (GRCm39)	missense	possibly damaging	0.93
R5590:Tenm4	UTSW	7	96,446,607 (GRCm39)	missense	possibly damaging	0.73
R5597:Tenm4	UTSW	7	96,202,724 (GRCm39)	missense	probably benign	0.00
R5782:Tenm4	UTSW	7	96,542,246 (GRCm39)	missense	probably benign	0.00
R5861:Tenm4	UTSW	7	96,492,424 (GRCm39)	intron	probably benign
R5890:Tenm4	UTSW	7	96,552,067 (GRCm39)	missense	probably damaging	1.00
R5930:Tenm4	UTSW	7	96,503,926 (GRCm39)	missense	probably damaging	1.00
R5940:Tenm4	UTSW	7	96,495,102 (GRCm39)	missense	probably damaging	1.00
R6012:Tenm4	UTSW	7	96,171,640 (GRCm39)	intron	probably benign
R6060:Tenm4	UTSW	7	96,522,918 (GRCm39)	missense	probably damaging	1.00
R6104:Tenm4	UTSW	7	96,486,496 (GRCm39)	missense	probably damaging	0.97
R6283:Tenm4	UTSW	7	96,523,701 (GRCm39)	missense	probably benign	0.33
R6333:Tenm4	UTSW	7	96,423,331 (GRCm39)	missense	probably damaging	1.00
R6522:Tenm4	UTSW	7	96,492,251 (GRCm39)	missense	possibly damaging	0.88
R6616:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6746:Tenm4	UTSW	7	96,542,067 (GRCm39)	missense	probably damaging	1.00
R6751:Tenm4	UTSW	7	96,494,919 (GRCm39)	missense	possibly damaging	0.95
R6806:Tenm4	UTSW	7	96,461,166 (GRCm39)	missense	possibly damaging	0.95
R6807:Tenm4	UTSW	7	96,544,478 (GRCm39)	missense	probably damaging	1.00
R6807:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6809:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6810:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6811:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6853:Tenm4	UTSW	7	96,486,502 (GRCm39)	missense	possibly damaging	0.94
R6886:Tenm4	UTSW	7	96,446,599 (GRCm39)	missense	possibly damaging	0.85
R6920:Tenm4	UTSW	7	96,544,757 (GRCm39)	missense	probably damaging	1.00
R6937:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R6939:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R7011:Tenm4	UTSW	7	96,545,342 (GRCm39)	nonsense	probably null
R7033:Tenm4	UTSW	7	96,544,430 (GRCm39)	nonsense	probably null
R7040:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R7083:Tenm4	UTSW	7	96,544,556 (GRCm39)	missense	probably damaging	1.00
R7238:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R7239:Tenm4	UTSW	7	96,385,020 (GRCm39)	missense	possibly damaging	0.47
R7239:Tenm4	UTSW	7	96,202,703 (GRCm39)	missense	probably benign	0.01
R7337:Tenm4	UTSW	7	96,523,333 (GRCm39)	missense	probably benign	0.44
R7400:Tenm4	UTSW	7	96,344,010 (GRCm39)	missense	probably damaging	0.97
R7449:Tenm4	UTSW	7	96,523,420 (GRCm39)	missense	possibly damaging	0.65
R7473:Tenm4	UTSW	7	96,423,353 (GRCm39)	missense	probably damaging	1.00
R7477:Tenm4	UTSW	7	96,495,015 (GRCm39)	missense	probably damaging	0.99
R7489:Tenm4	UTSW	7	96,486,521 (GRCm39)	missense	possibly damaging	0.90
R7498:Tenm4	UTSW	7	96,497,224 (GRCm39)	missense	probably damaging	1.00
R7562:Tenm4	UTSW	7	96,538,021 (GRCm39)	missense	probably damaging	1.00
R7615:Tenm4	UTSW	7	96,495,133 (GRCm39)	missense	probably damaging	1.00
R7624:Tenm4	UTSW	7	96,545,192 (GRCm39)	missense	possibly damaging	0.95
R7626:Tenm4	UTSW	7	96,542,221 (GRCm39)	missense	probably damaging	1.00
R7690:Tenm4	UTSW	7	96,512,740 (GRCm39)	missense	probably benign	0.00
R7692:Tenm4	UTSW	7	96,544,610 (GRCm39)	missense	probably damaging	1.00
R7748:Tenm4	UTSW	7	96,543,909 (GRCm39)	missense	probably damaging	1.00
R7763:Tenm4	UTSW	7	96,544,899 (GRCm39)	missense	probably benign	0.38
R7792:Tenm4	UTSW	7	96,423,221 (GRCm39)	missense	possibly damaging	0.54
R7855:Tenm4	UTSW	7	96,523,081 (GRCm39)	missense	probably damaging	1.00
R7868:Tenm4	UTSW	7	96,555,587 (GRCm39)	missense	possibly damaging	0.79
R7878:Tenm4	UTSW	7	96,501,564 (GRCm39)	missense	probably damaging	1.00
R7997:Tenm4	UTSW	7	96,523,512 (GRCm39)	missense	probably benign	0.44
R8017:Tenm4	UTSW	7	96,353,248 (GRCm39)	missense	probably damaging	1.00
R8019:Tenm4	UTSW	7	96,353,248 (GRCm39)	missense	probably damaging	1.00
R8054:Tenm4	UTSW	7	96,378,553 (GRCm39)	splice site	probably benign
R8061:Tenm4	UTSW	7	96,501,663 (GRCm39)	missense	probably damaging	1.00
R8108:Tenm4	UTSW	7	96,503,935 (GRCm39)	missense	probably benign	0.39
R8140:Tenm4	UTSW	7	96,544,383 (GRCm39)	missense	probably damaging	1.00
R8214:Tenm4	UTSW	7	96,544,614 (GRCm39)	missense	probably damaging	1.00
R8258:Tenm4	UTSW	7	96,517,198 (GRCm39)	missense	probably damaging	1.00
R8259:Tenm4	UTSW	7	96,517,198 (GRCm39)	missense	probably damaging	1.00
R8364:Tenm4	UTSW	7	96,421,313 (GRCm39)	critical splice donor site	probably null
R8542:Tenm4	UTSW	7	96,461,139 (GRCm39)	missense	probably damaging	0.99
R8669:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8670:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8683:Tenm4	UTSW	7	96,552,064 (GRCm39)	missense	probably damaging	0.99
R8691:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8692:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8714:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8716:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8735:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8736:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8737:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8738:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8738:Tenm4	UTSW	7	96,523,047 (GRCm39)	missense	probably damaging	1.00
R8739:Tenm4	UTSW	7	96,555,148 (GRCm39)	missense	probably benign
R8776:Tenm4	UTSW	7	96,544,239 (GRCm39)	missense	probably damaging	1.00
R8776-TAIL:Tenm4	UTSW	7	96,544,239 (GRCm39)	missense	probably damaging	1.00
R8777:Tenm4	UTSW	7	96,545,244 (GRCm39)	missense	probably damaging	1.00
R8777-TAIL:Tenm4	UTSW	7	96,545,244 (GRCm39)	missense	probably damaging	1.00
R8817:Tenm4	UTSW	7	96,523,335 (GRCm39)	missense	probably benign	0.01
R8851:Tenm4	UTSW	7	96,501,710 (GRCm39)	missense	probably damaging	1.00
R8913:Tenm4	UTSW	7	96,351,952 (GRCm39)	splice site	probably benign
R8977:Tenm4	UTSW	7	96,461,177 (GRCm39)	missense	probably damaging	1.00
R9100:Tenm4	UTSW	7	96,495,061 (GRCm39)	missense	probably damaging	1.00
R9136:Tenm4	UTSW	7	96,473,125 (GRCm39)	missense	possibly damaging	0.69
R9163:Tenm4	UTSW	7	96,473,080 (GRCm39)	missense	probably damaging	1.00
R9188:Tenm4	UTSW	7	96,421,234 (GRCm39)	missense	probably damaging	1.00
R9195:Tenm4	UTSW	7	96,542,126 (GRCm39)	missense	probably damaging	1.00
R9217:Tenm4	UTSW	7	96,534,646 (GRCm39)	missense	probably damaging	1.00
R9344:Tenm4	UTSW	7	96,545,352 (GRCm39)	missense	probably damaging	1.00
R9414:Tenm4	UTSW	7	96,545,367 (GRCm39)	missense	probably benign
R9466:Tenm4	UTSW	7	96,199,252 (GRCm39)	missense	possibly damaging	0.79
R9559:Tenm4	UTSW	7	96,473,056 (GRCm39)	missense	probably benign
R9626:Tenm4	UTSW	7	96,545,345 (GRCm39)	missense	probably damaging	1.00
R9673:Tenm4	UTSW	7	96,517,196 (GRCm39)	missense	probably damaging	1.00
R9676:Tenm4	UTSW	7	96,544,638 (GRCm39)	missense	probably damaging	1.00
R9678:Tenm4	UTSW	7	96,386,619 (GRCm39)	missense	possibly damaging	0.94
R9775:Tenm4	UTSW	7	96,555,761 (GRCm39)	missense	possibly damaging	0.92
R9790:Tenm4	UTSW	7	96,538,046 (GRCm39)	missense	probably damaging	1.00
R9791:Tenm4	UTSW	7	96,538,046 (GRCm39)	missense	probably damaging	1.00
R9803:Tenm4	UTSW	7	96,202,685 (GRCm39)	missense	probably damaging	1.00
X0021:Tenm4	UTSW	7	96,523,116 (GRCm39)	nonsense	probably null
X0026:Tenm4	UTSW	7	96,517,294 (GRCm39)	missense	probably damaging	0.98
X0066:Tenm4	UTSW	7	96,544,001 (GRCm39)	missense	probably damaging	1.00
X0066:Tenm4	UTSW	7	96,497,237 (GRCm39)	missense	probably damaging	1.00
Z1176:Tenm4	UTSW	7	96,555,121 (GRCm39)	missense	probably benign	0.00
Z1177:Tenm4	UTSW	7	96,512,792 (GRCm39)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- TAAGCTGCAATGAACCATCGC -3'
(R):5'- CAGGTTGGCCTCTACCTTAG -3'

Sequencing Primer
(F):5'- CAATGAACCATCGCAGGGG -3'
(R):5'- TCTACCTTAGAGGCAGGCTG -3'

Posted On

2019-10-07