Mutagenetix > Incidental Mutation

Incidental Mutation 'R7409:Ddx60'

574928

Institutional Source

Beutler Lab

Gene Symbol

Ddx60

Ensembl Gene

ENSMUSG00000037921

Gene Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 60

Synonyms

MMRRC Submission

045490-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.145) question?

Stock #

R7409 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

61928087-62038244 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 61958578 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Lysine at position 488 (T488K)

Ref Sequence

ENSEMBL: ENSMUSP00000091197 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000070631] [ENSMUST00000093485] [ENSMUST00000154398]

AlphaFold

E9PZQ1

Predicted Effect

probably benign
Transcript: ENSMUST00000070631
AA Change: T488K

PolyPhen 2 Score 0.243 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000070741
Gene: ENSMUSG00000037921
AA Change: T488K

Domain	Start	End	E-Value	Type
low complexity region	99	110	N/A	INTRINSIC
low complexity region	364	376	N/A	INTRINSIC
DEXDc	758	949	1.05e-15	SMART
Blast:DEXDc	1007	1132	4e-24	BLAST
HELICc	1245	1328	4.35e-13	SMART
low complexity region	1362	1373	N/A	INTRINSIC
Blast:DEXDc	1503	1584	1e-20	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000093485
AA Change: T488K

PolyPhen 2 Score 0.243 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000091197
Gene: ENSMUSG00000037921
AA Change: T488K

Domain	Start	End	E-Value	Type
low complexity region	99	110	N/A	INTRINSIC
low complexity region	364	376	N/A	INTRINSIC
DEXDc	759	950	1.05e-15	SMART
Blast:DEXDc	1008	1133	4e-24	BLAST
HELICc	1246	1329	4.35e-13	SMART
low complexity region	1363	1374	N/A	INTRINSIC
Blast:DEXDc	1504	1585	1e-20	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000154398
AA Change: T488K

PolyPhen 2 Score 0.243 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000122841
Gene: ENSMUSG00000037921
AA Change: T488K

Domain	Start	End	E-Value	Type
low complexity region	99	110	N/A	INTRINSIC
low complexity region	364	376	N/A	INTRINSIC

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.6%

Validation Efficiency

98% (82/84)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit normal immunity to several viruses (IAV, EMCV, SINV) but increased susceptibility to VSV infection. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610301B20Rik	T	A	4: 10,881,834 (GRCm38)	C94S	probably benign	Het
2610507B11Rik	G	T	11: 78,268,757 (GRCm38)	R544L	probably damaging	Het
Adgrb2	C	T	4: 130,019,069 (GRCm38)	A1329V	probably benign	Het
Aida	A	G	1: 183,318,874 (GRCm38)	T215A	probably benign	Het
Alpk2	A	G	18: 65,306,952 (GRCm38)	S457P	probably benign	Het
Ap4b1	G	A	3: 103,812,158 (GRCm38)	V63I	probably damaging	Het
Apaf1	A	G	10: 91,067,246 (GRCm38)	V182A	probably damaging	Het
B4galnt4	G	A	7: 141,067,003 (GRCm38)		probably null	Het
Cacna1a	T	G	8: 84,533,402 (GRCm38)	D331E	probably damaging	Het
Carmil2	A	G	8: 105,692,791 (GRCm38)		probably null	Het
Cdkn1b	A	T	6: 134,921,317 (GRCm38)	Q133L	probably benign	Het
Cep192	G	C	18: 67,834,803 (GRCm38)	S786T	possibly damaging	Het
Cfap97	G	A	8: 46,192,696 (GRCm38)	R537H	probably benign	Het
Clpx	C	G	9: 65,324,247 (GRCm38)	A552G	possibly damaging	Het
Cryl1	G	A	14: 57,286,385 (GRCm38)	T240I	probably damaging	Het
Dennd4b	A	G	3: 90,273,952 (GRCm38)	H805R	probably benign	Het
Dnmbp	T	C	19: 43,890,557 (GRCm38)	D25G	unknown	Het
Dysf	A	C	6: 84,149,682 (GRCm38)	D1293A	probably benign	Het
Efl1	T	C	7: 82,697,913 (GRCm38)	L549P	probably damaging	Het
Eif5	T	C	12: 111,540,263 (GRCm38)		probably benign	Het
Eva1c	A	G	16: 90,869,656 (GRCm38)	K156E	probably damaging	Het
Fbxw10	T	G	11: 62,876,780 (GRCm38)	V814G	possibly damaging	Het
Gfap	C	T	11: 102,894,532 (GRCm38)	R206Q	probably benign	Het
Gjb6	A	T	14: 57,124,153 (GRCm38)	L217*	probably null	Het
Gpatch11	T	A	17: 78,839,166 (GRCm38)	L80Q	probably damaging	Het
Gramd1b	T	C	9: 40,327,431 (GRCm38)	Q225R	probably damaging	Het
Gsdmc2	A	G	15: 63,833,346 (GRCm38)	S173P	possibly damaging	Het
Hars	C	G	18: 36,770,113 (GRCm38)	R388P	probably damaging	Het
Ighm	C	T	12: 113,422,232 (GRCm38)	R129H		Het
Igsf9	A	G	1: 172,495,274 (GRCm38)	I602V	probably benign	Het
Inpp4b	T	G	8: 81,952,685 (GRCm38)		probably null	Het
Itch	C	A	2: 155,199,382 (GRCm38)	T450K	probably damaging	Het
Kcnq1	T	C	7: 143,109,415 (GRCm38)	F20L	unknown	Het
Kmt2d	G	T	15: 98,855,354 (GRCm38)	A153E	probably damaging	Het
Macf1	T	A	4: 123,504,470 (GRCm38)	N750I	probably damaging	Het
Marveld2	T	A	13: 100,611,476 (GRCm38)	H365L	probably damaging	Het
Med13l	T	A	5: 118,754,321 (GRCm38)	D1936E	probably benign	Het
Mettl8	A	T	2: 70,973,343 (GRCm38)	V200E	probably damaging	Het
Mrgbp	T	G	2: 180,585,342 (GRCm38)	S157A	possibly damaging	Het
Mrps35	A	G	6: 147,055,983 (GRCm38)	T169A	possibly damaging	Het
Mycbp2	A	T	14: 103,288,744 (GRCm38)	Y551N	probably damaging	Het
Myo18b	T	C	5: 112,874,105 (GRCm38)	R474G	probably benign	Het
Nfx1	T	A	4: 41,021,830 (GRCm38)	S979R	possibly damaging	Het
Nlrp1a	T	C	11: 71,122,808 (GRCm38)	T539A	probably benign	Het
Oca2	T	A	7: 56,414,397 (GRCm38)	D713E	probably benign	Het
Olfr433	T	A	1: 174,042,533 (GRCm38)	H194Q	probably benign	Het
Olfr60	C	T	7: 140,345,405 (GRCm38)	V195I	probably benign	Het
Olfr768	A	G	10: 129,093,212 (GRCm38)	I254T	probably damaging	Het
Olfr816	A	T	10: 129,912,251 (GRCm38)	V9D	possibly damaging	Het
Omt2b	A	C	9: 78,328,612 (GRCm38)	Y73S	probably benign	Het
Pde11a	G	T	2: 76,005,984 (GRCm38)	Q20K		Het
Peg10	CCACATCAGGATCCACATCAGGATGCACATCAGCATCAGGATCCCCATCAGGATGCACATCAGGATCCACATCAGGATGCACATCAG	CCACATCAGGATCCACATCAGGATGCACATCAG	6: 4,756,398 (GRCm38)		probably benign	Het
Phrf1	C	A	7: 141,259,292 (GRCm38)	T800K	unknown	Het
Pramel4	T	C	4: 144,068,491 (GRCm38)	S486P	probably benign	Het
Proc	T	C	18: 32,127,460 (GRCm38)	D204G	probably benign	Het
Rasgrp3	T	A	17: 75,516,416 (GRCm38)	I494N	possibly damaging	Het
Samm50	A	G	15: 84,197,030 (GRCm38)	D53G	probably benign	Het
Satb1	T	C	17: 51,809,189 (GRCm38)	D22G	possibly damaging	Het
Scarf2	T	C	16: 17,807,054 (GRCm38)	S658P	probably damaging	Het
Sfta2	T	A	17: 35,614,518 (GRCm38)	I29K	unknown	Het
Slc15a4	A	T	5: 127,604,678 (GRCm38)	S292T	probably benign	Het
Slc37a1	C	T	17: 31,340,263 (GRCm38)	T439I	probably damaging	Het
Slc4a9	C	A	18: 36,530,805 (GRCm38)	P294Q	probably damaging	Het
Slc52a3	T	C	2: 152,004,166 (GRCm38)	S16P	probably damaging	Het
Slc6a15	A	G	10: 103,408,302 (GRCm38)	I468V	probably benign	Het
Spag17	A	T	3: 100,034,159 (GRCm38)	D738V	probably benign	Het
Spag17	T	C	3: 100,027,231 (GRCm38)	S610P	possibly damaging	Het
Ssbp4	T	C	8: 70,597,967 (GRCm38)	R269G	unknown	Het
Tbl1xr1	A	G	3: 22,203,190 (GRCm38)	T406A	possibly damaging	Het
Tep1	A	T	14: 50,866,855 (GRCm38)	V194D	possibly damaging	Het
Thbs4	A	T	13: 92,773,259 (GRCm38)	C343*	probably null	Het
Tmed10	A	T	12: 85,344,291 (GRCm38)	S158T	possibly damaging	Het
Trbv3	T	A	6: 41,048,590 (GRCm38)	V38E	probably damaging	Het
Ttc6	T	C	12: 57,696,986 (GRCm38)	M1258T	probably damaging	Het
Ttn	T	C	2: 76,758,976 (GRCm38)	D21281G	probably damaging	Het
Twistnb	T	G	12: 33,436,989 (GRCm38)	C150W	possibly damaging	Het
Usp54	T	C	14: 20,552,245 (GRCm38)	R1346G	probably damaging	Het
Vmn2r8	C	A	5: 108,808,583 (GRCm38)	E58*	probably null	Het
Vps13d	C	T	4: 145,141,254 (GRCm38)	E2009K		Het
Vps33b	T	C	7: 80,285,269 (GRCm38)	I320T	probably damaging	Het
Vwa8	A	C	14: 78,982,234 (GRCm38)		probably null	Het
Ythdf1	T	C	2: 180,911,993 (GRCm38)	Y143C	probably damaging	Het
Zfp213	C	T	17: 23,559,629 (GRCm38)		probably null	Het
Zfp219	A	T	14: 52,007,113 (GRCm38)	Y536*	probably null	Het

Other mutations in Ddx60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Ddx60	APN	8	61,958,646 (GRCm38)	missense	probably damaging	1.00
IGL00915:Ddx60	APN	8	61,987,431 (GRCm38)	missense	possibly damaging	0.79
IGL00931:Ddx60	APN	8	61,969,583 (GRCm38)	missense	probably benign	0.18
IGL01023:Ddx60	APN	8	61,942,514 (GRCm38)	missense	probably damaging	0.99
IGL01313:Ddx60	APN	8	61,982,526 (GRCm38)	missense	probably damaging	1.00
IGL01615:Ddx60	APN	8	61,963,740 (GRCm38)	missense	probably null	0.81
IGL01733:Ddx60	APN	8	61,983,865 (GRCm38)	missense	probably damaging	0.99
IGL01779:Ddx60	APN	8	62,017,823 (GRCm38)	missense	possibly damaging	0.94
IGL01900:Ddx60	APN	8	62,000,709 (GRCm38)	splice site	probably benign
IGL02110:Ddx60	APN	8	62,017,247 (GRCm38)	critical splice donor site	probably null
IGL02302:Ddx60	APN	8	61,975,832 (GRCm38)	missense	possibly damaging	0.85
IGL02468:Ddx60	APN	8	61,958,642 (GRCm38)	missense	probably damaging	1.00
IGL02569:Ddx60	APN	8	62,024,951 (GRCm38)	missense	possibly damaging	0.93
IGL02622:Ddx60	APN	8	61,942,436 (GRCm38)	splice site	probably null
IGL02657:Ddx60	APN	8	61,984,115 (GRCm38)	missense	probably benign	0.01
IGL02677:Ddx60	APN	8	61,988,132 (GRCm38)	missense	probably damaging	1.00
IGL02701:Ddx60	APN	8	61,979,341 (GRCm38)	missense	probably damaging	0.96
IGL02806:Ddx60	APN	8	61,956,122 (GRCm38)	missense	probably benign	0.00
IGL03137:Ddx60	APN	8	61,988,083 (GRCm38)	missense	possibly damaging	0.61
IGL03295:Ddx60	APN	8	61,956,121 (GRCm38)	missense	possibly damaging	0.82
IGL03387:Ddx60	APN	8	62,012,449 (GRCm38)	missense	probably damaging	1.00
IGL03411:Ddx60	APN	8	61,977,882 (GRCm38)	critical splice acceptor site	probably null
Scatter	UTSW	8	62,021,314 (GRCm38)	missense	possibly damaging	0.80
shotgun	UTSW	8	62,037,067 (GRCm38)	missense	probably benign	0.28
splay	UTSW	8	62,021,309 (GRCm38)	missense	possibly damaging	0.80
G1Funyon:Ddx60	UTSW	8	62,000,597 (GRCm38)	missense	probably benign	0.01
PIT4504001:Ddx60	UTSW	8	61,958,113 (GRCm38)	missense	probably benign
PIT4677001:Ddx60	UTSW	8	61,972,254 (GRCm38)	missense	possibly damaging	0.87
R0090:Ddx60	UTSW	8	61,942,293 (GRCm38)	missense	probably damaging	1.00
R0266:Ddx60	UTSW	8	62,033,493 (GRCm38)	missense	possibly damaging	0.92
R0325:Ddx60	UTSW	8	61,983,855 (GRCm38)	missense	probably benign	0.00
R0367:Ddx60	UTSW	8	62,017,749 (GRCm38)	missense	possibly damaging	0.78
R0403:Ddx60	UTSW	8	61,994,541 (GRCm38)	splice site	probably benign
R0479:Ddx60	UTSW	8	61,969,657 (GRCm38)	missense	probably damaging	1.00
R0561:Ddx60	UTSW	8	62,017,794 (GRCm38)	missense	possibly damaging	0.93
R0844:Ddx60	UTSW	8	61,987,361 (GRCm38)	missense	probably benign	0.27
R1119:Ddx60	UTSW	8	61,942,544 (GRCm38)	missense	probably damaging	1.00
R1428:Ddx60	UTSW	8	61,958,159 (GRCm38)	splice site	probably benign
R1778:Ddx60	UTSW	8	61,974,176 (GRCm38)	missense	possibly damaging	0.85
R1840:Ddx60	UTSW	8	61,969,553 (GRCm38)	missense	probably damaging	0.99
R1964:Ddx60	UTSW	8	61,948,869 (GRCm38)	missense	probably benign	0.10
R1970:Ddx60	UTSW	8	61,972,206 (GRCm38)	missense	possibly damaging	0.93
R2101:Ddx60	UTSW	8	61,940,645 (GRCm38)	missense	probably damaging	1.00
R2174:Ddx60	UTSW	8	62,017,200 (GRCm38)	missense	probably benign	0.01
R2174:Ddx60	UTSW	8	61,956,141 (GRCm38)	missense	probably damaging	1.00
R2198:Ddx60	UTSW	8	61,958,063 (GRCm38)	missense	possibly damaging	0.79
R2332:Ddx60	UTSW	8	62,037,091 (GRCm38)	missense	probably benign	0.08
R2338:Ddx60	UTSW	8	62,012,436 (GRCm38)	missense	possibly damaging	0.91
R2379:Ddx60	UTSW	8	62,037,088 (GRCm38)	missense	probably damaging	1.00
R4010:Ddx60	UTSW	8	61,956,144 (GRCm38)	missense	probably benign	0.25
R4010:Ddx60	UTSW	8	61,954,535 (GRCm38)	missense	possibly damaging	0.65
R4133:Ddx60	UTSW	8	61,972,220 (GRCm38)	missense	probably damaging	0.99
R4282:Ddx60	UTSW	8	61,994,393 (GRCm38)	missense	probably damaging	0.99
R4382:Ddx60	UTSW	8	61,948,978 (GRCm38)	splice site	probably null
R4561:Ddx60	UTSW	8	61,942,461 (GRCm38)	missense	probably damaging	0.96
R4572:Ddx60	UTSW	8	61,987,421 (GRCm38)	missense	probably damaging	1.00
R4581:Ddx60	UTSW	8	62,023,261 (GRCm38)	missense	possibly damaging	0.90
R4635:Ddx60	UTSW	8	62,037,067 (GRCm38)	missense	probably benign	0.28
R4698:Ddx60	UTSW	8	62,012,424 (GRCm38)	missense	probably benign	0.01
R4807:Ddx60	UTSW	8	61,979,338 (GRCm38)	missense	probably damaging	1.00
R4858:Ddx60	UTSW	8	62,021,314 (GRCm38)	missense	possibly damaging	0.80
R4964:Ddx60	UTSW	8	61,979,338 (GRCm38)	missense	probably damaging	1.00
R5120:Ddx60	UTSW	8	61,945,906 (GRCm38)	missense	probably benign	0.01
R5187:Ddx60	UTSW	8	61,974,188 (GRCm38)	missense	probably damaging	1.00
R5222:Ddx60	UTSW	8	61,984,158 (GRCm38)	missense	probably damaging	0.99
R5400:Ddx60	UTSW	8	62,010,002 (GRCm38)	missense	possibly damaging	0.65
R5500:Ddx60	UTSW	8	61,950,451 (GRCm38)	missense	probably benign	0.28
R5514:Ddx60	UTSW	8	61,958,057 (GRCm38)	missense	probably damaging	1.00
R5668:Ddx60	UTSW	8	62,000,578 (GRCm38)	missense	probably benign	0.38
R5742:Ddx60	UTSW	8	61,948,921 (GRCm38)	missense	probably benign
R5772:Ddx60	UTSW	8	61,948,897 (GRCm38)	missense	probably damaging	1.00
R5810:Ddx60	UTSW	8	62,012,388 (GRCm38)	nonsense	probably null
R5815:Ddx60	UTSW	8	61,963,722 (GRCm38)	missense	probably damaging	0.98
R5820:Ddx60	UTSW	8	61,956,121 (GRCm38)	missense	possibly damaging	0.82
R5866:Ddx60	UTSW	8	61,940,740 (GRCm38)	missense	probably damaging	1.00
R5881:Ddx60	UTSW	8	62,037,070 (GRCm38)	missense	probably damaging	1.00
R5977:Ddx60	UTSW	8	62,021,410 (GRCm38)	critical splice donor site	probably null
R6048:Ddx60	UTSW	8	62,000,582 (GRCm38)	missense	probably benign	0.01
R6061:Ddx60	UTSW	8	62,023,241 (GRCm38)	missense	probably null	0.01
R6153:Ddx60	UTSW	8	61,945,940 (GRCm38)	missense	possibly damaging	0.47
R6287:Ddx60	UTSW	8	61,950,578 (GRCm38)	missense	probably damaging	1.00
R6415:Ddx60	UTSW	8	61,983,905 (GRCm38)	missense	probably benign	0.00
R6416:Ddx60	UTSW	8	61,998,681 (GRCm38)	missense	probably benign
R6416:Ddx60	UTSW	8	61,977,950 (GRCm38)	missense	probably benign	0.00
R6660:Ddx60	UTSW	8	61,956,239 (GRCm38)	missense	probably benign	0.00
R6694:Ddx60	UTSW	8	62,037,070 (GRCm38)	missense	probably damaging	1.00
R6715:Ddx60	UTSW	8	61,983,890 (GRCm38)	missense	probably benign	0.03
R6720:Ddx60	UTSW	8	62,000,689 (GRCm38)	missense	probably benign	0.10
R6937:Ddx60	UTSW	8	62,037,069 (GRCm38)	missense	probably damaging	1.00
R7153:Ddx60	UTSW	8	61,988,108 (GRCm38)	missense	possibly damaging	0.71
R7274:Ddx60	UTSW	8	61,940,108 (GRCm38)	critical splice donor site	probably null
R7464:Ddx60	UTSW	8	61,940,674 (GRCm38)	missense	possibly damaging	0.82
R7670:Ddx60	UTSW	8	61,975,792 (GRCm38)	missense	probably damaging	1.00
R7904:Ddx60	UTSW	8	61,977,890 (GRCm38)	missense	possibly damaging	0.81
R7992:Ddx60	UTSW	8	61,954,535 (GRCm38)	missense	probably benign	0.03
R8124:Ddx60	UTSW	8	61,983,911 (GRCm38)	missense	probably benign
R8125:Ddx60	UTSW	8	61,983,911 (GRCm38)	missense	probably benign
R8126:Ddx60	UTSW	8	61,983,911 (GRCm38)	missense	probably benign
R8155:Ddx60	UTSW	8	62,017,171 (GRCm38)	missense	possibly damaging	0.61
R8174:Ddx60	UTSW	8	62,017,250 (GRCm38)	splice site	probably null
R8192:Ddx60	UTSW	8	61,977,968 (GRCm38)	missense	probably damaging	1.00
R8271:Ddx60	UTSW	8	61,940,108 (GRCm38)	critical splice donor site	probably null
R8301:Ddx60	UTSW	8	62,000,597 (GRCm38)	missense	probably benign	0.01
R8304:Ddx60	UTSW	8	61,998,769 (GRCm38)	missense	possibly damaging	0.67
R8319:Ddx60	UTSW	8	61,942,635 (GRCm38)	critical splice donor site	probably null
R8374:Ddx60	UTSW	8	61,974,171 (GRCm38)	missense	probably benign	0.01
R8401:Ddx60	UTSW	8	61,956,243 (GRCm38)	missense	possibly damaging	0.57
R8487:Ddx60	UTSW	8	61,974,150 (GRCm38)	missense	probably damaging	1.00
R8804:Ddx60	UTSW	8	61,958,606 (GRCm38)	missense	probably benign	0.27
R8826:Ddx60	UTSW	8	61,945,956 (GRCm38)	missense	probably benign	0.02
R8829:Ddx60	UTSW	8	61,940,661 (GRCm38)	missense	probably damaging	1.00
R8881:Ddx60	UTSW	8	62,021,309 (GRCm38)	missense	possibly damaging	0.80
R8884:Ddx60	UTSW	8	61,994,519 (GRCm38)	missense	possibly damaging	0.86
R8990:Ddx60	UTSW	8	61,974,134 (GRCm38)	nonsense	probably null
R9122:Ddx60	UTSW	8	61,989,864 (GRCm38)	missense	probably benign	0.16
R9225:Ddx60	UTSW	8	62,017,841 (GRCm38)	missense	probably benign	0.36
R9278:Ddx60	UTSW	8	61,977,978 (GRCm38)	missense	possibly damaging	0.83
R9293:Ddx60	UTSW	8	62,009,960 (GRCm38)	missense	possibly damaging	0.89
R9405:Ddx60	UTSW	8	61,972,214 (GRCm38)	missense	probably benign	0.03
R9766:Ddx60	UTSW	8	62,012,278 (GRCm38)	missense	probably damaging	1.00
X0003:Ddx60	UTSW	8	62,033,417 (GRCm38)	missense	possibly damaging	0.88
X0019:Ddx60	UTSW	8	61,963,692 (GRCm38)	missense	probably benign	0.01
Z1177:Ddx60	UTSW	8	62,000,588 (GRCm38)	missense	possibly damaging	0.92

Predicted Primers

PCR Primer
(F):5'- GGAGGTCCTGGTTTCTGAAAC -3'
(R):5'- CGTGTCACACATCTGAATGCC -3'

Sequencing Primer
(F):5'- CTTGTATCCCAGGAAGAAG -3'
(R):5'- GTCACACATCTGAATGCCTTAATGAC -3'

Posted On

2019-10-07