|Institutional Source||Beutler Lab|
|Gene Name||suppressor of Ty 16|
|Synonyms||Supt16h, Spt16, Fact140, Cdc68|
|Is this an essential gene?||Probably essential (E-score: 0.968)|
|Stock #||R7411 (G1)|
|Chromosomal Location||52160414-52197416 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||A to T at 52178051 bp (GRCm38)|
|Amino Acid Change||Valine to Glutamic Acid at position 409 (V409E)|
|Ref Sequence||ENSEMBL: ENSMUSP00000042283 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000046709]|
|AlphaFold||no structure available at present|
AA Change: V409E
PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
AA Change: V409E
|Coding Region Coverage||
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Supt16||
(F):5'- GTGATAAGGACATCGAATGCCC -3'
(R):5'- CCGTGAAGGCTCTCTAGTAATC -3'
(F):5'- GGACATCGAATGCCCCTTCATTC -3'
(R):5'- CGTGAAGGCTCTCTAGTAATCAATAG -3'