Incidental Mutation 'R7413:Wscd2'
ID 575212
Institutional Source Beutler Lab
Gene Symbol Wscd2
Ensembl Gene ENSMUSG00000063430
Gene Name WSC domain containing 2
Synonyms 4933413A10Rik, C530024P05Rik
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R7413 (G1)
Quality Score 225.009
Status Not validated
Chromosome 5
Chromosomal Location 113638199-113727786 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 113715402 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Threonine at position 414 (I414T)
Ref Sequence ENSEMBL: ENSMUSP00000092021 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000094452]
AlphaFold D4PHA7
Predicted Effect probably benign
Transcript: ENSMUST00000094452
AA Change: I414T

PolyPhen 2 Score 0.018 (Sensitivity: 0.95; Specificity: 0.80)
SMART Domains Protein: ENSMUSP00000092021
Gene: ENSMUSG00000063430
AA Change: I414T

DomainStartEndE-ValueType
transmembrane domain 21 43 N/A INTRINSIC
low complexity region 44 61 N/A INTRINSIC
WSC 133 225 6.11e-44 SMART
WSC 236 330 9.49e-41 SMART
Blast:WSC 389 409 2e-6 BLAST
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.2%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 62 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A1cf T C 19: 31,895,524 (GRCm39) probably null Het
Agbl4 A T 4: 111,514,495 (GRCm39) D502V probably benign Het
Akap5 T A 12: 76,375,678 (GRCm39) V370E possibly damaging Het
Alms1 T C 6: 85,605,288 (GRCm39) C1844R probably benign Het
Apaf1 T C 10: 90,831,542 (GRCm39) K1191E probably benign Het
Bsn C T 9: 108,016,690 (GRCm39) R107Q possibly damaging Het
Catsperb G T 12: 101,447,307 (GRCm39) R269L probably damaging Het
Ccer1 T C 10: 97,529,804 (GRCm39) S156P unknown Het
Cdh16 C T 8: 105,346,572 (GRCm39) A241T probably benign Het
Cdk5rap2 A G 4: 70,172,972 (GRCm39) S1367P probably damaging Het
Cds2 C T 2: 132,135,235 (GRCm39) P42L probably benign Het
Col15a1 T C 4: 47,245,431 (GRCm39) Y61H possibly damaging Het
Dclre1a G A 19: 56,531,082 (GRCm39) P755S probably damaging Het
Ddi1 A T 9: 6,265,670 (GRCm39) M233K probably damaging Het
Dpp4 T C 2: 62,187,333 (GRCm39) Y474C probably damaging Het
Epha7 T A 4: 28,871,838 (GRCm39) M389K probably benign Het
Ephb3 T C 16: 21,033,457 (GRCm39) F181S probably damaging Het
Epor G A 9: 21,874,776 (GRCm39) probably benign Het
Fbp2 C A 13: 62,985,067 (GRCm39) V285L probably benign Het
Flnc A T 6: 29,452,258 (GRCm39) D1694V probably damaging Het
Ganab T A 19: 8,882,339 (GRCm39) M98K probably benign Het
Gcm2 A G 13: 41,259,230 (GRCm39) S80P probably damaging Het
Gls A G 1: 52,254,735 (GRCm39) S247P probably benign Het
Gne T C 4: 44,044,857 (GRCm39) N426D probably benign Het
Ifne A T 4: 88,797,840 (GRCm39) *193R probably null Het
Igf2r G T 17: 12,917,115 (GRCm39) S1595* probably null Het
Krt26 C T 11: 99,225,887 (GRCm39) M226I probably benign Het
Lars2 G A 9: 123,288,568 (GRCm39) V805I probably benign Het
Lrrc57 C A 2: 120,436,577 (GRCm39) R177L probably damaging Het
Lrrn1 A G 6: 107,546,083 (GRCm39) E627G probably benign Het
Magel2 CCCTCCTCCTCCTCCTCCTCCT CCCTCCTCCTCCTCCTCCT 7: 62,027,592 (GRCm39) probably benign Het
Mdga1 A T 17: 30,069,647 (GRCm39) V133E probably damaging Het
Med12l A G 3: 58,998,971 (GRCm39) T644A probably benign Het
Meis1 T A 11: 18,938,357 (GRCm39) D218V probably damaging Het
Myo15b A G 11: 115,768,970 (GRCm39) E1439G Het
Naa12 T C 18: 80,254,874 (GRCm39) V56A possibly damaging Het
Nckipsd G A 9: 108,691,280 (GRCm39) V401I probably benign Het
Nrg3 T C 14: 38,092,669 (GRCm39) I655V probably damaging Het
Oprm1 A T 10: 6,778,919 (GRCm39) I107F probably damaging Het
Or4k42 A C 2: 111,319,933 (GRCm39) I190R probably benign Het
Or52n3 A T 7: 104,530,057 (GRCm39) I48F probably benign Het
Or5p51 T C 7: 107,444,721 (GRCm39) D73G probably damaging Het
Osbpl7 T C 11: 96,945,704 (GRCm39) probably null Het
Pcdh18 T G 3: 49,699,232 (GRCm39) S1077R possibly damaging Het
Pcdhb16 A T 18: 37,611,975 (GRCm39) K312* probably null Het
Plekhh2 A T 17: 84,873,724 (GRCm39) E336D probably benign Het
Ptprd A T 4: 76,165,076 (GRCm39) S49T probably benign Het
Pxdn A G 12: 30,052,927 (GRCm39) I1035V probably benign Het
Rlf C T 4: 121,007,297 (GRCm39) G671D probably damaging Het
Rsf1 GGCGGCGGC GGCGGCGGCCGCGGCGGC 7: 97,229,128 (GRCm39) probably benign Het
Selenbp2 A G 3: 94,607,404 (GRCm39) Q275R probably benign Het
Slc10a1 G T 12: 81,007,396 (GRCm39) F128L probably benign Het
Slitrk1 A T 14: 109,149,357 (GRCm39) Y451* probably null Het
Spata31f3 TCATTCAACACTTTGGAGAGCTCTGAACTCTGGCCATTCAACACTTTGGAGAGCTCTGAACTCTGGCCATTCAACACTTTGGAGAGCTCTGAACTCTGGTCATTCAACACTTTGG TCATTCAACACTTTGGAGAGCTCTGAACTCTGGCCATTCAACACTTTGGAGAGCTCTGAACTCTGGTCATTCAACACTTTGG 4: 42,871,823 (GRCm39) probably benign Het
Stambpl1 G C 19: 34,204,116 (GRCm39) G69R probably damaging Het
Surf4 G T 2: 26,814,455 (GRCm39) R149S probably benign Het
Tmem151a T C 19: 5,132,702 (GRCm39) Y168C probably damaging Het
Ttc39c A G 18: 12,861,746 (GRCm39) K416R possibly damaging Het
Tut4 T C 4: 108,406,533 (GRCm39) I1367T possibly damaging Het
Vit T C 17: 78,932,309 (GRCm39) I472T probably damaging Het
Vmn1r212 A C 13: 23,067,718 (GRCm39) L205R probably damaging Het
Zfp1005 A C 2: 150,108,081 (GRCm39) T14P possibly damaging Het
Other mutations in Wscd2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00323:Wscd2 APN 5 113,689,236 (GRCm39) missense possibly damaging 0.56
IGL01113:Wscd2 APN 5 113,708,800 (GRCm39) missense probably damaging 1.00
IGL01476:Wscd2 APN 5 113,710,382 (GRCm39) missense probably damaging 0.99
IGL01894:Wscd2 APN 5 113,710,357 (GRCm39) missense probably damaging 0.96
IGL02560:Wscd2 APN 5 113,699,045 (GRCm39) missense probably benign 0.01
IGL02755:Wscd2 APN 5 113,712,092 (GRCm39) missense possibly damaging 0.66
froggie UTSW 5 113,726,206 (GRCm39) makesense probably null
R0381:Wscd2 UTSW 5 113,689,192 (GRCm39) missense probably damaging 0.96
R1144:Wscd2 UTSW 5 113,699,151 (GRCm39) splice site probably null
R1858:Wscd2 UTSW 5 113,689,231 (GRCm39) missense possibly damaging 0.63
R3695:Wscd2 UTSW 5 113,689,078 (GRCm39) missense possibly damaging 0.82
R4231:Wscd2 UTSW 5 113,699,045 (GRCm39) missense probably benign 0.00
R4232:Wscd2 UTSW 5 113,699,045 (GRCm39) missense probably benign 0.00
R4667:Wscd2 UTSW 5 113,715,333 (GRCm39) missense probably damaging 1.00
R4696:Wscd2 UTSW 5 113,689,240 (GRCm39) missense probably benign
R5727:Wscd2 UTSW 5 113,715,411 (GRCm39) missense possibly damaging 0.89
R6401:Wscd2 UTSW 5 113,726,206 (GRCm39) makesense probably null
R7642:Wscd2 UTSW 5 113,715,475 (GRCm39) missense possibly damaging 0.67
R7837:Wscd2 UTSW 5 113,710,394 (GRCm39) missense probably damaging 1.00
R8046:Wscd2 UTSW 5 113,689,176 (GRCm39) missense probably benign 0.05
R8331:Wscd2 UTSW 5 113,688,996 (GRCm39) start codon destroyed probably null 0.99
R8348:Wscd2 UTSW 5 113,710,371 (GRCm39) missense possibly damaging 0.89
R8680:Wscd2 UTSW 5 113,712,235 (GRCm39) missense probably damaging 1.00
R8928:Wscd2 UTSW 5 113,715,401 (GRCm39) missense possibly damaging 0.90
R9125:Wscd2 UTSW 5 113,715,417 (GRCm39) missense probably benign 0.33
Predicted Primers PCR Primer
(F):5'- AGGTGCTCAGAATACACACTGG -3'
(R):5'- ACTGCTGCTTTGCACAGCTC -3'

Sequencing Primer
(F):5'- TGCTCAGAATACACACTGGAAGGG -3'
(R):5'- TGCTTTGCACAGCTCTCTTAAGAG -3'
Posted On 2019-10-07