Mutagenetix > Incidental Mutation

Incidental Mutation 'R7424:Olfr262'

575957

Institutional Source

Beutler Lab

Gene Symbol

Olfr262

Ensembl Gene

ENSMUSG00000067519

Gene Name

olfactory receptor 262

Synonyms

MOR214-1, GA_x6K02T2N4A9-18144-19082, MOR214-9

MMRRC Submission

045502-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.090) question?

Stock #

R7424 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

12240721-12241659 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 12240954 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 236 (S236T)

Ref Sequence

ENSEMBL: ENSMUSP00000085120 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087818]

AlphaFold

Q8VFV8

Predicted Effect

possibly damaging
Transcript: ENSMUST00000087818
AA Change: S236T

PolyPhen 2 Score 0.648 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000085120
Gene: ENSMUSG00000067519
AA Change: S236T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	309	2.3e-55	PFAM
Pfam:7tm_1	42	291	1.2e-20	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

99% (76/77)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6430548M08Rik	C	T	8: 120,145,545 (GRCm38)	R71C	probably damaging	Het
Aanat	A	T	11: 116,595,629 (GRCm38)		probably benign	Het
Ahrr	G	T	13: 74,257,545 (GRCm38)	S91*	probably null	Het
Ampd1	A	T	3: 103,088,442 (GRCm38)	N223Y	probably benign	Het
Ankar	T	G	1: 72,680,058 (GRCm38)	N544T	probably damaging	Het
Ankk1	A	G	9: 49,418,750 (GRCm38)	S302P	possibly damaging	Het
Bcr	T	C	10: 75,157,100 (GRCm38)	V809A	probably benign	Het
Bpifb2	A	G	2: 153,890,540 (GRCm38)	N353S	possibly damaging	Het
Cyth1	A	T	11: 118,184,009 (GRCm38)		probably null	Het
Ddx5	A	T	11: 106,782,180 (GRCm38)	N506K	probably benign	Het
Dnaja1	A	T	4: 40,730,244 (GRCm38)	I239F	probably benign	Het
Ethe1	C	T	7: 24,606,251 (GRCm38)	T141I	probably damaging	Het
Fam160b2	T	C	14: 70,594,007 (GRCm38)	H29R	probably damaging	Het
Gdap2	A	T	3: 100,202,066 (GRCm38)	I36F	unknown	Het
Gm13030	A	T	4: 138,871,266 (GRCm38)	D115E	unknown	Het
Gm13103	C	T	4: 143,853,209 (GRCm38)	P455S	probably benign	Het
Gm17019	A	T	5: 15,029,372 (GRCm38)	L227Q	probably damaging	Het
Gm9195	T	A	14: 72,435,777 (GRCm38)	E2517D	possibly damaging	Het
Gramd2	T	A	9: 59,708,071 (GRCm38)	V39D	possibly damaging	Het
Hmcn1	C	T	1: 150,630,266 (GRCm38)	W3836*	probably null	Het
Hspa14	C	T	2: 3,489,041 (GRCm38)	D494N	possibly damaging	Het
Ifit2	A	G	19: 34,573,198 (GRCm38)	N46S	probably benign	Het
Ifna6	A	T	4: 88,827,807 (GRCm38)	E131V	possibly damaging	Het
Ift140	T	C	17: 25,037,036 (GRCm38)	V504A	possibly damaging	Het
Irgc1	T	C	7: 24,432,228 (GRCm38)	N388S	probably damaging	Het
Itgal	T	A	7: 127,317,365 (GRCm38)	V743E	probably benign	Het
Itih5	T	C	2: 10,245,637 (GRCm38)	S716P	probably damaging	Het
Kcnab1	A	T	3: 65,266,503 (GRCm38)	K78N	possibly damaging	Het
Kif1a	A	T	1: 93,054,317 (GRCm38)	V787E	possibly damaging	Het
Krt15	A	T	11: 100,135,560 (GRCm38)	V100E	possibly damaging	Het
Krt39	A	T	11: 99,518,091 (GRCm38)	V293E	probably damaging	Het
Lrrn4	A	G	2: 132,869,743 (GRCm38)	F720S	possibly damaging	Het
Map2	G	A	1: 66,414,824 (GRCm38)	A958T	possibly damaging	Het
Map3k9	A	G	12: 81,724,097 (GRCm38)	S906P	probably benign	Het
Mdc1	T	C	17: 35,853,309 (GRCm38)	S1250P	probably benign	Het
Meltf	G	A	16: 31,884,946 (GRCm38)	V164I	probably damaging	Het
Mtap	T	G	4: 89,179,462 (GRCm38)		probably null	Het
Mtus1	C	A	8: 41,022,406 (GRCm38)	V184F	probably damaging	Het
Myh1	A	T	11: 67,213,663 (GRCm38)	D1015V	probably damaging	Het
Ndrg1	T	C	15: 66,944,938 (GRCm38)		probably null	Het
Nkd1	G	T	8: 88,585,175 (GRCm38)	V130L	probably benign	Het
Nsfl1c	A	G	2: 151,500,753 (GRCm38)	D81G	probably benign	Het
Nt5c1b	T	A	12: 10,381,391 (GRCm38)		probably null	Het
Nucb1	T	C	7: 45,498,778 (GRCm38)	K204E	possibly damaging	Het
Nwd1	T	G	8: 72,675,173 (GRCm38)	M774R	possibly damaging	Het
Olfr165	A	T	16: 19,407,194 (GRCm38)	V274E	probably damaging	Het
Olfr654	G	C	7: 104,588,700 (GRCm38)	E299Q	probably damaging	Het
Pan3	T	A	5: 147,536,272 (GRCm38)		probably null	Het
Pcdh15	A	T	10: 74,506,485 (GRCm38)	T1135S	probably benign	Het
Pfas	A	G	11: 69,000,092 (GRCm38)	I331T	probably damaging	Het
Plxna2	T	A	1: 194,806,339 (GRCm38)	I1641N	probably damaging	Het
Ptar1	A	T	19: 23,718,101 (GRCm38)	R311W	probably damaging	Het
Ranbp2	T	G	10: 58,479,194 (GRCm38)	M1912R	probably damaging	Het
Rbm12	A	G	2: 156,097,303 (GRCm38)	F350L	possibly damaging	Het
Sdhaf1	T	C	7: 30,322,043 (GRCm38)	D96G	probably benign	Het
Serpinb6b	T	A	13: 32,968,667 (GRCm38)	M53K	probably damaging	Het
Sh2d6	A	T	6: 72,517,164 (GRCm38)	L147Q	probably benign	Het
Slc19a2	T	A	1: 164,260,876 (GRCm38)	C298S	probably benign	Het
Son	AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	AGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCGCAGGAGCCGAACCCCCAGCCG	16: 91,660,334 (GRCm38)		probably benign	Het
St8sia2	T	A	7: 73,960,902 (GRCm38)	Q211L	possibly damaging	Het
Sult2a2	T	C	7: 13,734,897 (GRCm38)	I96T	possibly damaging	Het
Tab2	G	T	10: 7,907,483 (GRCm38)	H678Q	probably damaging	Het
Tnfaip6	A	G	2: 52,038,216 (GRCm38)	E14G	probably benign	Het
Trip11	A	T	12: 101,885,198 (GRCm38)	L869H	probably damaging	Het
Tslp	T	C	18: 32,819,080 (GRCm38)	Y133H	not run	Het
Ttn	T	C	2: 76,740,990 (GRCm38)	I26520V	probably damaging	Het
Ttn	A	G	2: 76,932,143 (GRCm38)	V3374A	unknown	Het
Tubgcp2	T	A	7: 140,007,924 (GRCm38)	I263F	possibly damaging	Het
Uaca	A	G	9: 60,870,110 (GRCm38)	E593G	probably damaging	Het
Unc13b	C	T	4: 43,172,235 (GRCm38)	T1021I	unknown	Het
Ush1c	T	A	7: 46,225,555 (GRCm38)	I131F	probably benign	Het
Usp24	C	A	4: 106,379,107 (GRCm38)	D997E	probably benign	Het
Usp54	T	C	14: 20,577,040 (GRCm38)	T517A	probably benign	Het
Vmn1r151	A	T	7: 22,499,080 (GRCm38)	M200K	possibly damaging	Het
Vmn2r43	T	C	7: 8,255,329 (GRCm38)	D295G	probably damaging	Het
Vmn2r70	G	A	7: 85,563,868 (GRCm38)	P444S	probably damaging	Het
Vmn2r85	G	T	10: 130,418,980 (GRCm38)	P612T	probably damaging	Het
Vps13d	A	T	4: 145,148,747 (GRCm38)	V1736D		Het
Zbtb11	A	T	16: 55,990,487 (GRCm38)	H336L	probably benign	Het

Other mutations in Olfr262

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01432:Olfr262	APN	19	12,241,527 (GRCm38)	missense	probably damaging	0.99
IGL02170:Olfr262	APN	19	12,240,756 (GRCm38)	missense	probably benign
IGL02748:Olfr262	APN	19	12,240,840 (GRCm38)	missense	probably benign	0.01
IGL02896:Olfr262	APN	19	12,240,989 (GRCm38)	nonsense	probably null
R0365:Olfr262	UTSW	19	12,241,076 (GRCm38)	missense	probably benign	0.13
R0374:Olfr262	UTSW	19	12,241,141 (GRCm38)	missense	probably damaging	1.00
R1226:Olfr262	UTSW	19	12,241,586 (GRCm38)	missense	probably benign
R1319:Olfr262	UTSW	19	12,241,502 (GRCm38)	missense	probably damaging	1.00
R1426:Olfr262	UTSW	19	12,241,182 (GRCm38)	missense	possibly damaging	0.81
R1453:Olfr262	UTSW	19	12,241,592 (GRCm38)	missense	probably benign
R1675:Olfr262	UTSW	19	12,240,831 (GRCm38)	missense	probably benign	0.37
R1773:Olfr262	UTSW	19	12,241,659 (GRCm38)	start codon destroyed	probably null	0.03
R1778:Olfr262	UTSW	19	12,241,455 (GRCm38)	missense	probably benign
R1820:Olfr262	UTSW	19	12,241,248 (GRCm38)	missense	probably damaging	1.00
R3161:Olfr262	UTSW	19	12,241,496 (GRCm38)	missense	probably benign	0.06
R3412:Olfr262	UTSW	19	12,241,590 (GRCm38)	missense	probably benign	0.00
R4387:Olfr262	UTSW	19	12,241,139 (GRCm38)	missense	probably damaging	0.98
R4389:Olfr262	UTSW	19	12,241,139 (GRCm38)	missense	probably damaging	0.98
R4782:Olfr262	UTSW	19	12,241,572 (GRCm38)	missense	probably benign	0.01
R4885:Olfr262	UTSW	19	12,240,718 (GRCm38)	splice site	probably null
R4915:Olfr262	UTSW	19	12,241,373 (GRCm38)	missense	probably benign	0.31
R5254:Olfr262	UTSW	19	12,241,248 (GRCm38)	missense	probably damaging	1.00
R5726:Olfr262	UTSW	19	12,241,280 (GRCm38)	missense	probably damaging	0.99
R6579:Olfr262	UTSW	19	12,241,362 (GRCm38)	missense	probably benign
R7062:Olfr262	UTSW	19	12,240,725 (GRCm38)	missense	probably benign
R9491:Olfr262	UTSW	19	12,241,242 (GRCm38)	missense	probably benign	0.01
R9736:Olfr262	UTSW	19	12,241,556 (GRCm38)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- GTGTCCCTAGTTGCAGATTGTC -3'
(R):5'- GTGACATGCCACAGTTGTTAG -3'

Sequencing Primer
(F):5'- CTGTTCTTCAGACTGTATATCAATGG -3'
(R):5'- ACATGCCACAGTTGTTAGTTCTG -3'

Posted On

2019-10-07