Mutagenetix > Incidental Mutation

Incidental Mutation 'R7425:Vcan'

576014

Institutional Source

Beutler Lab

Gene Symbol

Vcan

Ensembl Gene

ENSMUSG00000021614

Gene Name

versican

Synonyms

PG-M, hdf, DPEAAE, heart defect, Cspg2, 5430420N07Rik

MMRRC Submission

045503-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7425 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

89655312-89742509 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 89689832 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 2531 (I2531T)

Ref Sequence

ENSEMBL: ENSMUSP00000105173 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000109543] [ENSMUST00000109544] [ENSMUST00000109546] [ENSMUST00000159910]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000109543

SMART Domains

Protein: ENSMUSP00000105170
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
EGF	351	384	2.72e-7	SMART
EGF_CA	386	422	1.16e-10	SMART
CLECT	428	549	3.08e-34	SMART
CCP	555	611	1.04e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109544

SMART Domains

Protein: ENSMUSP00000105171
Gene: ENSMUSG00000021614

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
EGF	1311	1344	2.72e-7	SMART
EGF_CA	1346	1382	1.16e-10	SMART
CLECT	1388	1509	3.08e-34	SMART
CCP	1515	1571	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000109546
AA Change: I2531T

PolyPhen 2 Score 0.988 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000105173
Gene: ENSMUSG00000021614
AA Change: I2531T

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	728	743	N/A	INTRINSIC
low complexity region	1205	1219	N/A	INTRINSIC
low complexity region	1322	1333	N/A	INTRINSIC
low complexity region	1546	1569	N/A	INTRINSIC
low complexity region	1837	1852	N/A	INTRINSIC
low complexity region	2013	2026	N/A	INTRINSIC
low complexity region	2354	2367	N/A	INTRINSIC
low complexity region	2468	2482	N/A	INTRINSIC
low complexity region	2719	2728	N/A	INTRINSIC
EGF	3050	3083	2.72e-7	SMART
EGF_CA	3085	3121	1.16e-10	SMART
CLECT	3127	3248	3.08e-34	SMART
CCP	3254	3310	1.04e-8	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000159910
AA Change: I1571T

PolyPhen 2 Score 0.968 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000125446
Gene: ENSMUSG00000021614
AA Change: I1571T

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
IG	29	148	1.4e-7	SMART
LINK	148	245	1.4e-53	SMART
LINK	249	347	8.8e-60	SMART
low complexity region	362	373	N/A	INTRINSIC
low complexity region	586	609	N/A	INTRINSIC
low complexity region	877	892	N/A	INTRINSIC
low complexity region	1053	1066	N/A	INTRINSIC
low complexity region	1394	1407	N/A	INTRINSIC
low complexity region	1508	1522	N/A	INTRINSIC
low complexity region	1759	1768	N/A	INTRINSIC
EGF	2090	2123	2.72e-7	SMART
EGF_CA	2125	2161	1.16e-10	SMART
CLECT	2167	2288	3.08e-34	SMART
CCP	2294	2350	1.04e-8	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the aggrecan/versican proteoglycan family. The protein encoded is a large chondroitin sulfate proteoglycan and is a major component of the extracellular matrix. This protein is involved in cell adhesion, proliferation, proliferation, migration and angiogenesis and plays a central role in tissue morphogenesis and maintenance. Mutations in this gene are the cause of Wagner syndrome type 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]
PHENOTYPE: Homozygotes for an insertional mutation exhibit anterior-posterior segmental defects of the heart, lack endocardial cushions of the conus and atrioventricular region, and die and around embryonic day 10.5. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 138,065,695 (GRCm38)	E215G	probably benign	Het
4932438A13Rik	A	T	3: 36,983,394 (GRCm38)	H2448L	probably benign	Het
4932438A13Rik	T	A	3: 36,948,341 (GRCm38)	H1478Q	probably benign	Het
Adam8	T	A	7: 139,992,481 (GRCm38)		probably benign	Het
Ankrd35	A	G	3: 96,684,788 (GRCm38)	S797G	not run	Het
Anxa3	T	A	5: 96,834,821 (GRCm38)	H259Q	probably benign	Het
Ap3d1	T	A	10: 80,721,592 (GRCm38)	Q302L	probably damaging	Het
Atg2a	T	C	19: 6,255,652 (GRCm38)	V1294A	probably benign	Het
Atp13a5	G	A	16: 29,297,460 (GRCm38)	Q613*	probably null	Het
Bmpr2	T	G	1: 59,867,351 (GRCm38)	N534K	probably benign	Het
C1qbp	G	T	11: 70,978,247 (GRCm38)		probably null	Het
C1qbp	T	C	11: 70,978,246 (GRCm38)		probably null	Het
C1ql3	T	G	2: 13,010,418 (GRCm38)	K144Q	possibly damaging	Het
C3	T	C	17: 57,204,039 (GRCm38)	M1656V	possibly damaging	Het
Cand1	A	T	10: 119,216,243 (GRCm38)	Y252N	probably benign	Het
Capn13	T	C	17: 73,318,058 (GRCm38)	I632M	probably benign	Het
Catsperb	A	G	12: 101,591,498 (GRCm38)	E776G	probably damaging	Het
Ccnl1	A	T	3: 65,948,758 (GRCm38)	V242D	probably damaging	Het
Cd68	T	C	11: 69,665,112 (GRCm38)	D200G	probably benign	Het
Cela3a	T	C	4: 137,405,588 (GRCm38)	N118D	probably benign	Het
Celsr2	A	G	3: 108,402,457 (GRCm38)	C1609R	probably damaging	Het
Cep85l	G	C	10: 53,301,570 (GRCm38)	Q458E	probably damaging	Het
Cnga1	T	G	5: 72,609,525 (GRCm38)	E190D	probably benign	Het
Cntnap3	G	A	13: 64,758,252 (GRCm38)	R847C	probably damaging	Het
Cntrob	G	A	11: 69,314,734 (GRCm38)	Q425*	probably null	Het
Commd9	G	A	2: 101,899,900 (GRCm38)	W128*	probably null	Het
Csnk1a1	C	T	18: 61,585,259 (GRCm38)	S352L	unknown	Het
Dact2	A	G	17: 14,196,331 (GRCm38)	S536P	probably damaging	Het
Ddx25	A	G	9: 35,554,586 (GRCm38)	I113T	probably benign	Het
Dscam	T	A	16: 96,629,398 (GRCm38)	D1630V	probably damaging	Het
Fndc7	A	C	3: 108,876,659 (GRCm38)	F211L	probably benign	Het
Fryl	T	C	5: 73,104,748 (GRCm38)	T559A	probably damaging	Het
Hpx	C	A	7: 105,591,861 (GRCm38)	D402Y	probably damaging	Het
Ints13	A	G	6: 146,574,700 (GRCm38)		probably null	Het
Ipcef1	T	C	10: 6,956,066 (GRCm38)	K76E	probably damaging	Het
Kcnb2	A	C	1: 15,709,807 (GRCm38)	Q301P	probably damaging	Het
Lamp3	C	T	16: 19,699,612 (GRCm38)		probably null	Het
Lmod2	A	T	6: 24,603,476 (GRCm38)	H150L	probably benign	Het
Lrch3	T	C	16: 33,005,707 (GRCm38)	F718S	probably damaging	Het
Mms22l	T	C	4: 24,596,287 (GRCm38)	V1082A	probably benign	Het
Nbas	G	T	12: 13,469,880 (GRCm38)	V1598L	probably damaging	Het
Nrxn3	C	T	12: 89,513,100 (GRCm38)	R671*	probably null	Het
Olfr1215	A	T	2: 89,002,200 (GRCm38)	F29L		Het
Olfr1441	A	C	19: 12,422,840 (GRCm38)	H177P	probably damaging	Het
Olfr214	A	T	6: 116,556,437 (GRCm38)	E4V	possibly damaging	Het
Olfr48	A	G	2: 89,844,445 (GRCm38)	L176P	probably damaging	Het
Olfr671	A	T	7: 104,975,061 (GRCm38)	L312*	probably null	Het
Pcdhga6	T	A	18: 37,708,566 (GRCm38)	N446K	probably damaging	Het
Phlpp1	A	T	1: 106,392,573 (GRCm38)	I1433F	probably benign	Het
Pla2g6	G	A	15: 79,308,733 (GRCm38)	R245C	probably damaging	Het
Rgl3	G	A	9: 21,976,827 (GRCm38)	Q464*	probably null	Het
Ryr2	T	C	13: 11,705,644 (GRCm38)	D2706G	probably benign	Het
Sbf2	G	A	7: 110,375,777 (GRCm38)	Q718*	probably null	Het
Sdad1	T	C	5: 92,300,121 (GRCm38)	T252A	probably benign	Het
Sgk1	T	C	10: 21,994,110 (GRCm38)	L16P	probably damaging	Het
Slc38a8	A	G	8: 119,485,588 (GRCm38)	S339P	possibly damaging	Het
Slc44a4	T	C	17: 34,921,691 (GRCm38)	S287P	possibly damaging	Het
Syne1	T	C	10: 5,425,760 (GRCm38)	I111V	probably damaging	Het
Synm	A	C	7: 67,733,446 (GRCm38)	S1489R	probably damaging	Het
Tfb2m	A	G	1: 179,537,704 (GRCm38)	F232L	probably benign	Het
Traf3	A	T	12: 111,260,661 (GRCm38)	K328*	probably null	Het
Trim38	C	A	13: 23,788,382 (GRCm38)	Q229K	probably benign	Het
Virma	T	A	4: 11,546,211 (GRCm38)	I1683N	possibly damaging	Het
Vmn1r18	A	T	6: 57,390,566 (GRCm38)	M1K	probably null	Het
Vmn2r87	T	C	10: 130,478,892 (GRCm38)	N275S	probably damaging	Het
Vps13a	T	A	19: 16,723,702 (GRCm38)	H701L	probably benign	Het
Vrk3	T	A	7: 44,770,924 (GRCm38)		probably null	Het
Zfp993	T	A	4: 146,657,641 (GRCm38)	S141T	possibly damaging	Het

Other mutations in Vcan

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00434:Vcan	APN	13	89,704,702 (GRCm38)	missense	probably damaging	1.00
IGL00502:Vcan	APN	13	89,692,319 (GRCm38)	missense	probably benign
IGL00504:Vcan	APN	13	89,691,275 (GRCm38)	missense	possibly damaging	0.70
IGL00566:Vcan	APN	13	89,688,979 (GRCm38)	missense	probably benign	0.01
IGL00701:Vcan	APN	13	89,703,726 (GRCm38)	missense	probably benign
IGL00743:Vcan	APN	13	89,725,306 (GRCm38)	missense	probably damaging	0.98
IGL00962:Vcan	APN	13	89,662,052 (GRCm38)	missense	probably damaging	1.00
IGL01085:Vcan	APN	13	89,679,958 (GRCm38)	missense	probably damaging	1.00
IGL01317:Vcan	APN	13	89,691,668 (GRCm38)	missense	probably benign	0.00
IGL01349:Vcan	APN	13	89,703,943 (GRCm38)	missense	probably damaging	0.98
IGL01391:Vcan	APN	13	89,704,169 (GRCm38)	missense	probably benign	0.19
IGL01644:Vcan	APN	13	89,688,675 (GRCm38)	missense	probably benign	0.13
IGL01657:Vcan	APN	13	89,690,586 (GRCm38)	missense	probably damaging	1.00
IGL01707:Vcan	APN	13	89,689,745 (GRCm38)	missense	probably damaging	1.00
IGL01764:Vcan	APN	13	89,725,388 (GRCm38)	missense	probably damaging	1.00
IGL01920:Vcan	APN	13	89,689,205 (GRCm38)	missense	probably benign	0.04
IGL01989:Vcan	APN	13	89,689,359 (GRCm38)	missense	possibly damaging	0.86
IGL01999:Vcan	APN	13	89,684,438 (GRCm38)	missense	probably damaging	1.00
IGL02083:Vcan	APN	13	89,725,565 (GRCm38)	missense	probably damaging	1.00
IGL02160:Vcan	APN	13	89,684,493 (GRCm38)	missense	probably damaging	1.00
IGL02217:Vcan	APN	13	89,703,077 (GRCm38)	missense	probably damaging	1.00
IGL02522:Vcan	APN	13	89,704,849 (GRCm38)	missense	probably benign	0.00
IGL02527:Vcan	APN	13	89,690,657 (GRCm38)	missense	possibly damaging	0.95
IGL02926:Vcan	APN	13	89,688,623 (GRCm38)	missense	probably damaging	0.98
IGL03061:Vcan	APN	13	89,703,275 (GRCm38)	missense	probably benign	0.25
IGL03331:Vcan	APN	13	89,661,932 (GRCm38)	missense	probably damaging	1.00
IGL03352:Vcan	APN	13	89,705,006 (GRCm38)	missense	probably benign	0.00
R0041:Vcan	UTSW	13	89,661,985 (GRCm38)	missense	probably damaging	1.00
R0102:Vcan	UTSW	13	89,703,668 (GRCm38)	missense	probably benign	0.01
R0102:Vcan	UTSW	13	89,703,668 (GRCm38)	missense	probably benign	0.01
R0109:Vcan	UTSW	13	89,678,073 (GRCm38)	critical splice donor site	probably null
R0139:Vcan	UTSW	13	89,691,261 (GRCm38)	missense	probably damaging	1.00
R0295:Vcan	UTSW	13	89,712,191 (GRCm38)	missense	probably benign	0.06
R0375:Vcan	UTSW	13	89,691,275 (GRCm38)	missense	probably damaging	0.99
R0379:Vcan	UTSW	13	89,703,546 (GRCm38)	missense	probably damaging	0.99
R0457:Vcan	UTSW	13	89,703,199 (GRCm38)	missense	possibly damaging	0.78
R0482:Vcan	UTSW	13	89,678,145 (GRCm38)	missense	probably damaging	1.00
R0485:Vcan	UTSW	13	89,704,660 (GRCm38)	missense	possibly damaging	0.92
R0532:Vcan	UTSW	13	89,703,772 (GRCm38)	missense	probably damaging	0.99
R0561:Vcan	UTSW	13	89,731,464 (GRCm38)	missense	possibly damaging	0.86
R0561:Vcan	UTSW	13	89,712,253 (GRCm38)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,712,267 (GRCm38)	missense	probably damaging	1.00
R0636:Vcan	UTSW	13	89,704,706 (GRCm38)	missense	probably damaging	0.99
R0680:Vcan	UTSW	13	89,679,822 (GRCm38)	missense	probably damaging	1.00
R0849:Vcan	UTSW	13	89,704,953 (GRCm38)	missense	possibly damaging	0.75
R1006:Vcan	UTSW	13	89,685,077 (GRCm38)	critical splice donor site	probably null
R1104:Vcan	UTSW	13	89,692,410 (GRCm38)	missense	probably damaging	1.00
R1118:Vcan	UTSW	13	89,705,663 (GRCm38)	missense	probably damaging	1.00
R1137:Vcan	UTSW	13	89,704,303 (GRCm38)	missense	probably damaging	1.00
R1199:Vcan	UTSW	13	89,679,794 (GRCm38)	splice site	probably null
R1219:Vcan	UTSW	13	89,679,904 (GRCm38)	missense	probably damaging	1.00
R1296:Vcan	UTSW	13	89,657,556 (GRCm38)	missense	probably damaging	1.00
R1332:Vcan	UTSW	13	89,693,055 (GRCm38)	missense	probably damaging	1.00
R1336:Vcan	UTSW	13	89,693,055 (GRCm38)	missense	probably damaging	1.00
R1403:Vcan	UTSW	13	89,688,484 (GRCm38)	missense	probably benign	0.00
R1403:Vcan	UTSW	13	89,688,484 (GRCm38)	missense	probably benign	0.00
R1546:Vcan	UTSW	13	89,692,956 (GRCm38)	missense	probably damaging	0.99
R1604:Vcan	UTSW	13	89,689,661 (GRCm38)	missense	probably benign	0.42
R1616:Vcan	UTSW	13	89,705,663 (GRCm38)	missense	probably damaging	1.00
R1636:Vcan	UTSW	13	89,703,667 (GRCm38)	missense	possibly damaging	0.90
R1654:Vcan	UTSW	13	89,661,946 (GRCm38)	missense	probably damaging	1.00
R1680:Vcan	UTSW	13	89,703,547 (GRCm38)	missense	probably benign	0.19
R1694:Vcan	UTSW	13	89,688,483 (GRCm38)	missense	probably damaging	0.98
R1712:Vcan	UTSW	13	89,721,775 (GRCm38)	missense	probably damaging	1.00
R1754:Vcan	UTSW	13	89,704,735 (GRCm38)	missense	probably benign	0.01
R1756:Vcan	UTSW	13	89,691,681 (GRCm38)	missense	probably benign	0.05
R1824:Vcan	UTSW	13	89,705,212 (GRCm38)	missense	possibly damaging	0.75
R1852:Vcan	UTSW	13	89,705,392 (GRCm38)	missense	probably damaging	0.99
R1868:Vcan	UTSW	13	89,690,871 (GRCm38)	missense	probably benign	0.12
R1920:Vcan	UTSW	13	89,693,015 (GRCm38)	missense	probably damaging	1.00
R1932:Vcan	UTSW	13	89,705,534 (GRCm38)	missense	possibly damaging	0.78
R1934:Vcan	UTSW	13	89,702,926 (GRCm38)	missense	probably damaging	1.00
R1942:Vcan	UTSW	13	89,703,424 (GRCm38)	missense	probably benign	0.01
R1964:Vcan	UTSW	13	89,692,742 (GRCm38)	missense	probably benign	0.02
R1970:Vcan	UTSW	13	89,689,038 (GRCm38)	missense	probably damaging	1.00
R2045:Vcan	UTSW	13	89,690,985 (GRCm38)	missense	probably benign	0.00
R2110:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2111:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2112:Vcan	UTSW	13	89,693,303 (GRCm38)	missense	probably damaging	1.00
R2136:Vcan	UTSW	13	89,689,737 (GRCm38)	missense	probably damaging	1.00
R2158:Vcan	UTSW	13	89,703,529 (GRCm38)	missense	possibly damaging	0.68
R2376:Vcan	UTSW	13	89,703,410 (GRCm38)	missense	possibly damaging	0.80
R2385:Vcan	UTSW	13	89,689,449 (GRCm38)	missense	probably damaging	1.00
R2443:Vcan	UTSW	13	89,704,675 (GRCm38)	missense	probably damaging	1.00
R2876:Vcan	UTSW	13	89,704,237 (GRCm38)	missense	probably damaging	1.00
R3607:Vcan	UTSW	13	89,703,301 (GRCm38)	missense	probably damaging	0.98
R4042:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4043:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4044:Vcan	UTSW	13	89,692,543 (GRCm38)	missense	probably benign	0.35
R4065:Vcan	UTSW	13	89,679,887 (GRCm38)	missense	probably damaging	1.00
R4161:Vcan	UTSW	13	89,685,158 (GRCm38)	missense	probably damaging	1.00
R4178:Vcan	UTSW	13	89,725,547 (GRCm38)	missense	probably damaging	1.00
R4290:Vcan	UTSW	13	89,725,486 (GRCm38)	missense	probably damaging	1.00
R4530:Vcan	UTSW	13	89,704,028 (GRCm38)	missense	probably damaging	0.97
R4666:Vcan	UTSW	13	89,679,934 (GRCm38)	missense	probably damaging	1.00
R4785:Vcan	UTSW	13	89,705,789 (GRCm38)	missense	probably damaging	1.00
R4870:Vcan	UTSW	13	89,704,739 (GRCm38)	missense	probably benign	0.01
R4973:Vcan	UTSW	13	89,688,842 (GRCm38)	missense	probably benign	0.30
R5037:Vcan	UTSW	13	89,703,977 (GRCm38)	missense	probably damaging	1.00
R5104:Vcan	UTSW	13	89,657,472 (GRCm38)	intron	probably benign
R5124:Vcan	UTSW	13	89,725,517 (GRCm38)	missense	probably damaging	1.00
R5129:Vcan	UTSW	13	89,690,240 (GRCm38)	missense	probably damaging	1.00
R5198:Vcan	UTSW	13	89,690,872 (GRCm38)	missense	probably damaging	1.00
R5240:Vcan	UTSW	13	89,692,532 (GRCm38)	missense	probably benign	0.08
R5254:Vcan	UTSW	13	89,691,600 (GRCm38)	missense	probably damaging	0.99
R5280:Vcan	UTSW	13	89,690,286 (GRCm38)	missense	probably benign	0.00
R5522:Vcan	UTSW	13	89,691,810 (GRCm38)	missense	possibly damaging	0.62
R5557:Vcan	UTSW	13	89,703,112 (GRCm38)	missense	possibly damaging	0.77
R5568:Vcan	UTSW	13	89,688,671 (GRCm38)	missense	probably damaging	1.00
R5578:Vcan	UTSW	13	89,691,503 (GRCm38)	missense	probably benign	0.01
R5627:Vcan	UTSW	13	89,691,135 (GRCm38)	frame shift	probably null
R5687:Vcan	UTSW	13	89,678,134 (GRCm38)	missense	probably damaging	1.00
R5752:Vcan	UTSW	13	89,679,950 (GRCm38)	missense	probably damaging	1.00
R5879:Vcan	UTSW	13	89,703,952 (GRCm38)	missense	probably damaging	0.99
R5941:Vcan	UTSW	13	89,692,691 (GRCm38)	missense	probably damaging	0.98
R6113:Vcan	UTSW	13	89,657,536 (GRCm38)	nonsense	probably null
R6135:Vcan	UTSW	13	89,689,926 (GRCm38)	missense	probably benign	0.36
R6252:Vcan	UTSW	13	89,691,220 (GRCm38)	nonsense	probably null
R6280:Vcan	UTSW	13	89,725,373 (GRCm38)	missense	probably damaging	1.00
R6317:Vcan	UTSW	13	89,691,597 (GRCm38)	missense	probably benign	0.22
R6327:Vcan	UTSW	13	89,704,832 (GRCm38)	missense	probably damaging	0.99
R6460:Vcan	UTSW	13	89,690,687 (GRCm38)	missense	possibly damaging	0.61
R6669:Vcan	UTSW	13	89,704,731 (GRCm38)	missense	probably benign	0.21
R6744:Vcan	UTSW	13	89,705,182 (GRCm38)	missense	probably damaging	1.00
R6819:Vcan	UTSW	13	89,705,125 (GRCm38)	missense	probably benign	0.00
R6880:Vcan	UTSW	13	89,712,381 (GRCm38)	missense	probably damaging	1.00
R6956:Vcan	UTSW	13	89,689,431 (GRCm38)	missense	probably damaging	0.99
R6971:Vcan	UTSW	13	89,678,133 (GRCm38)	missense	probably damaging	1.00
R6985:Vcan	UTSW	13	89,679,956 (GRCm38)	missense	probably damaging	1.00
R6994:Vcan	UTSW	13	89,693,407 (GRCm38)	missense	possibly damaging	0.94
R6997:Vcan	UTSW	13	89,690,618 (GRCm38)	missense	probably damaging	0.98
R7029:Vcan	UTSW	13	89,690,241 (GRCm38)	missense	probably damaging	1.00
R7066:Vcan	UTSW	13	89,705,686 (GRCm38)	missense	probably damaging	1.00
R7156:Vcan	UTSW	13	89,689,110 (GRCm38)	missense	possibly damaging	0.95
R7171:Vcan	UTSW	13	89,725,591 (GRCm38)	missense	probably damaging	1.00
R7176:Vcan	UTSW	13	89,688,936 (GRCm38)	missense	probably benign	0.01
R7229:Vcan	UTSW	13	89,705,270 (GRCm38)	missense	possibly damaging	0.87
R7250:Vcan	UTSW	13	89,731,457 (GRCm38)	critical splice donor site	probably null
R7250:Vcan	UTSW	13	89,721,686 (GRCm38)	missense	probably damaging	1.00
R7262:Vcan	UTSW	13	89,705,161 (GRCm38)	missense	possibly damaging	0.62
R7289:Vcan	UTSW	13	89,692,733 (GRCm38)	nonsense	probably null
R7299:Vcan	UTSW	13	89,705,266 (GRCm38)	missense	probably benign
R7301:Vcan	UTSW	13	89,705,266 (GRCm38)	missense	probably benign
R7514:Vcan	UTSW	13	89,704,118 (GRCm38)	missense	probably damaging	0.97
R7579:Vcan	UTSW	13	89,692,458 (GRCm38)	missense	probably damaging	1.00
R7618:Vcan	UTSW	13	89,692,223 (GRCm38)	missense	probably damaging	0.99
R7655:Vcan	UTSW	13	89,685,114 (GRCm38)	missense	probably damaging	1.00
R7656:Vcan	UTSW	13	89,685,114 (GRCm38)	missense	probably damaging	1.00
R7676:Vcan	UTSW	13	89,691,789 (GRCm38)	missense	probably damaging	1.00
R7719:Vcan	UTSW	13	89,704,619 (GRCm38)	missense	probably damaging	0.98
R7753:Vcan	UTSW	13	89,689,323 (GRCm38)	missense	probably damaging	1.00
R7762:Vcan	UTSW	13	89,692,937 (GRCm38)	missense	probably damaging	1.00
R7778:Vcan	UTSW	13	89,688,654 (GRCm38)	missense	probably damaging	1.00
R7824:Vcan	UTSW	13	89,688,654 (GRCm38)	missense	probably damaging	1.00
R7995:Vcan	UTSW	13	89,691,858 (GRCm38)	missense	probably benign
R7998:Vcan	UTSW	13	89,704,327 (GRCm38)	missense	probably damaging	1.00
R8033:Vcan	UTSW	13	89,704,360 (GRCm38)	missense	probably benign	0.04
R8061:Vcan	UTSW	13	89,657,290 (GRCm38)	missense	probably benign	0.45
R8103:Vcan	UTSW	13	89,703,320 (GRCm38)	nonsense	probably null
R8103:Vcan	UTSW	13	89,657,658 (GRCm38)	missense	probably damaging	1.00
R8124:Vcan	UTSW	13	89,704,254 (GRCm38)	missense	possibly damaging	0.93
R8162:Vcan	UTSW	13	89,704,987 (GRCm38)	nonsense	probably null
R8166:Vcan	UTSW	13	89,692,736 (GRCm38)	missense	probably benign	0.02
R8274:Vcan	UTSW	13	89,704,970 (GRCm38)	missense	probably benign	0.02
R8284:Vcan	UTSW	13	89,704,335 (GRCm38)	missense	possibly damaging	0.68
R8417:Vcan	UTSW	13	89,688,743 (GRCm38)	missense	probably benign	0.19
R8696:Vcan	UTSW	13	89,691,098 (GRCm38)	missense	probably benign	0.00
R8738:Vcan	UTSW	13	89,692,320 (GRCm38)	missense	probably benign	0.17
R8792:Vcan	UTSW	13	89,692,111 (GRCm38)	missense	possibly damaging	0.91
R8887:Vcan	UTSW	13	89,704,907 (GRCm38)	missense	probably benign
R9049:Vcan	UTSW	13	89,678,105 (GRCm38)	missense	probably damaging	1.00
R9074:Vcan	UTSW	13	89,691,027 (GRCm38)	missense	possibly damaging	0.95
R9095:Vcan	UTSW	13	89,704,525 (GRCm38)	missense	probably benign	0.32
R9172:Vcan	UTSW	13	89,679,931 (GRCm38)	missense	probably damaging	1.00
R9199:Vcan	UTSW	13	89,690,496 (GRCm38)	nonsense	probably null
R9259:Vcan	UTSW	13	89,690,870 (GRCm38)	missense	probably damaging	0.99
R9455:Vcan	UTSW	13	89,689,333 (GRCm38)	missense	probably damaging	1.00
R9476:Vcan	UTSW	13	89,703,412 (GRCm38)	missense	possibly damaging	0.95
R9477:Vcan	UTSW	13	89,693,009 (GRCm38)	missense	probably damaging	1.00
R9555:Vcan	UTSW	13	89,691,540 (GRCm38)	missense
R9579:Vcan	UTSW	13	89,689,594 (GRCm38)	missense	possibly damaging	0.67
R9606:Vcan	UTSW	13	89,705,372 (GRCm38)	missense	probably damaging	1.00
R9645:Vcan	UTSW	13	89,692,962 (GRCm38)	missense	probably benign	0.00
R9659:Vcan	UTSW	13	89,691,741 (GRCm38)	missense	probably damaging	0.99
R9766:Vcan	UTSW	13	89,691,128 (GRCm38)	missense	probably benign	0.00
R9778:Vcan	UTSW	13	89,689,811 (GRCm38)	missense	probably damaging	1.00
X0058:Vcan	UTSW	13	89,692,493 (GRCm38)	missense	probably benign	0.21
X0065:Vcan	UTSW	13	89,705,749 (GRCm38)	missense	probably damaging	0.96
Z1176:Vcan	UTSW	13	89,692,571 (GRCm38)	missense	probably benign	0.10
Z1177:Vcan	UTSW	13	89,704,073 (GRCm38)	missense	probably damaging	1.00
Z1177:Vcan	UTSW	13	89,703,788 (GRCm38)	nonsense	probably null
Z1177:Vcan	UTSW	13	89,703,524 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GAGAAAGGGTAACAGCTCCC -3'
(R):5'- AAGAGTTCTCTGTATCCAACCAGC -3'

Sequencing Primer
(F):5'- CATACTTCTCTTGAACTTGAAGGC -3'
(R):5'- TCTCTGTATCCAACCAGCACACTG -3'

Posted On

2019-10-07