Mutagenetix > Incidental Mutation

Incidental Mutation 'R7437:Zfp932'

576666

Institutional Source

Beutler Lab

Gene Symbol

Zfp932

Ensembl Gene

ENSMUSG00000066613

Gene Name

zinc finger protein 932

Synonyms

2310001H12Rik

MMRRC Submission

045513-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R7437 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

110144387-110158277 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 110157880 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 526 (M526K)

Ref Sequence

ENSEMBL: ENSMUSP00000108159 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099484] [ENSMUST00000112536] [ENSMUST00000112540] [ENSMUST00000125213] [ENSMUST00000143639] [ENSMUST00000187241]

AlphaFold

E9QAG8

Predicted Effect

probably benign
Transcript: ENSMUST00000099484

SMART Domains

Protein: ENSMUSP00000097083
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112536
AA Change: M493K

PolyPhen 2 Score 0.096 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000108155
Gene: ENSMUSG00000066613
AA Change: M493K

Domain	Start	End	E-Value	Type
Blast:KRAB	1	31	5e-12	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
ZnF_C2H2	183	205	1.3e-4	SMART
ZnF_C2H2	211	233	1.1e-2	SMART
ZnF_C2H2	239	261	2.27e-4	SMART
ZnF_C2H2	267	289	1.84e-4	SMART
ZnF_C2H2	295	317	5.21e-4	SMART
ZnF_C2H2	323	345	9.73e-4	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	1.58e-3	SMART
ZnF_C2H2	407	429	5.14e-3	SMART
ZnF_C2H2	435	457	2.61e-4	SMART
ZnF_C2H2	463	485	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112540
AA Change: M526K

PolyPhen 2 Score 0.096 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000108159
Gene: ENSMUSG00000066613
AA Change: M526K

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART
ZnF_C2H2	104	126	1.1e-2	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	3.39e-3	SMART
ZnF_C2H2	188	210	1.76e-1	SMART
ZnF_C2H2	216	238	1.3e-4	SMART
ZnF_C2H2	244	266	1.1e-2	SMART
ZnF_C2H2	272	294	2.27e-4	SMART
ZnF_C2H2	300	322	1.84e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	9.73e-4	SMART
ZnF_C2H2	384	406	1.58e-3	SMART
ZnF_C2H2	412	434	1.58e-3	SMART
ZnF_C2H2	440	462	5.14e-3	SMART
ZnF_C2H2	468	490	2.61e-4	SMART
ZnF_C2H2	496	518	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125213

SMART Domains

Protein: ENSMUSP00000119843
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	2e-14	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
Pfam:zf-C2H2_6	182	191	7.3e-1	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000143639

SMART Domains

Protein: ENSMUSP00000121749
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	4	60	1.74e-14	SMART
ZnF_C2H2	103	125	1.1e-2	SMART
ZnF_C2H2	131	153	5.21e-4	SMART
ZnF_C2H2	159	181	3.39e-3	SMART
ZnF_C2H2	187	209	1.76e-1	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000187241
AA Change: M526K

PolyPhen 2 Score 0.652 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000140446
Gene: ENSMUSG00000066613
AA Change: M526K

Domain	Start	End	E-Value	Type
KRAB	5	61	7.1e-17	SMART
ZnF_C2H2	104	126	4.8e-5	SMART
ZnF_C2H2	132	154	2.2e-6	SMART
ZnF_C2H2	160	182	1.5e-5	SMART
ZnF_C2H2	188	210	7.2e-4	SMART
ZnF_C2H2	216	238	5.4e-7	SMART
ZnF_C2H2	244	266	4.5e-5	SMART
ZnF_C2H2	272	294	9.2e-7	SMART
ZnF_C2H2	300	322	7.5e-7	SMART
ZnF_C2H2	328	350	2.1e-6	SMART
ZnF_C2H2	356	378	4e-6	SMART
ZnF_C2H2	384	406	6.5e-6	SMART
ZnF_C2H2	412	434	6.9e-6	SMART
ZnF_C2H2	440	462	2.1e-5	SMART
ZnF_C2H2	468	490	1.1e-6	SMART
ZnF_C2H2	496	518	1.1e-6	SMART

Meta Mutation Damage Score

0.0846

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

98% (54/55)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca3	A	T	17: 24,619,472 (GRCm39)	I1192F	probably damaging	Het
Abca8a	A	G	11: 109,941,790 (GRCm39)	S1160P	probably benign	Het
Adamtsl2	A	G	2: 26,979,721 (GRCm39)	I297V	probably damaging	Het
Aebp1	T	A	11: 5,819,757 (GRCm39)	F687L	possibly damaging	Het
Ahsa1	A	G	12: 87,314,930 (GRCm39)	T28A	probably damaging	Het
Akap3	A	T	6: 126,842,618 (GRCm39)	K412N	probably damaging	Het
Atl1	A	G	12: 69,978,396 (GRCm39)	I123V	probably benign	Het
Atp10a	G	A	7: 58,308,288 (GRCm39)	R29H	unknown	Het
C4b	T	C	17: 34,953,707 (GRCm39)	D967G	probably benign	Het
Ccdc186	T	C	19: 56,795,429 (GRCm39)	N416S	probably damaging	Het
Ccna2	T	C	3: 36,625,239 (GRCm39)		probably benign	Het
Cep70	T	C	9: 99,173,582 (GRCm39)	L371P	probably damaging	Het
Cfap46	A	T	7: 139,230,753 (GRCm39)	C958*	probably null	Het
Crlf2	T	C	5: 109,702,839 (GRCm39)	D318G	probably benign	Het
Csf1	T	C	3: 107,658,072 (GRCm39)	N14D	probably benign	Het
Dars1	A	G	1: 128,299,941 (GRCm39)	Y348H	possibly damaging	Het
Dnah2	T	C	11: 69,389,453 (GRCm39)	E813G	probably damaging	Het
Ebi3	T	A	17: 56,261,410 (GRCm39)	M102K	probably benign	Het
Epg5	A	G	18: 78,066,493 (GRCm39)	D2131G	probably benign	Het
Fam186a	A	T	15: 99,840,775 (GRCm39)	L1823H	probably damaging	Het
H2-M3	T	C	17: 37,583,569 (GRCm39)	M309T	probably benign	Het
Ift122	C	T	6: 115,903,263 (GRCm39)	R1176C	probably benign	Het
Ino80	A	G	2: 119,273,067 (GRCm39)	S470P	possibly damaging	Het
Kcnh5	C	T	12: 75,184,417 (GRCm39)		probably null	Het
Kif24	T	C	4: 41,404,687 (GRCm39)	T438A	possibly damaging	Het
Kndc1	G	A	7: 139,488,959 (GRCm39)	G205R	probably damaging	Het
Lrp1b	G	T	2: 40,712,658 (GRCm39)	D3225E		Het
Lrp1b	A	T	2: 40,712,657 (GRCm39)	Y3226N		Het
Megf10	G	C	18: 57,395,203 (GRCm39)	G522R	probably damaging	Het
Nectin2	A	T	7: 19,483,193 (GRCm39)	L12*	probably null	Het
Ngef	A	T	1: 87,408,327 (GRCm39)	M580K	probably damaging	Het
Oga	A	T	19: 45,767,046 (GRCm39)	M110K	possibly damaging	Het
Or10ag56	A	G	2: 87,139,687 (GRCm39)	M205V	probably benign	Het
Or1e1c	A	G	11: 73,265,844 (GRCm39)	S93G	probably benign	Het
Or5an1b	T	C	19: 12,299,472 (GRCm39)	N240D	probably damaging	Het
Pcdhb13	T	C	18: 37,577,728 (GRCm39)	L702P	probably damaging	Het
Pcdhb17	A	G	18: 37,619,145 (GRCm39)	I312V	probably benign	Het
Pglyrp3	A	G	3: 91,937,985 (GRCm39)	N265D	probably benign	Het
Pkd1l2	A	G	8: 117,757,421 (GRCm39)	V1539A	probably damaging	Het
Prxl2b	C	A	4: 154,981,053 (GRCm39)	C194F	possibly damaging	Het
Rcn2	T	C	9: 55,965,353 (GRCm39)	L274P	probably damaging	Het
Rpp30	A	G	19: 36,081,838 (GRCm39)	E267G	possibly damaging	Het
Rundc3a	A	G	11: 102,289,230 (GRCm39)	M134V	probably damaging	Het
Samd3	A	T	10: 26,146,004 (GRCm39)	Q343L	possibly damaging	Het
Serpinb3c	A	T	1: 107,199,444 (GRCm39)	F359Y	probably damaging	Het
Slc9a9	T	C	9: 95,110,994 (GRCm39)	L604P	probably benign	Het
Strn3	A	T	12: 51,656,946 (GRCm39)	H777Q	probably damaging	Het
Tap1	G	A	17: 34,409,616 (GRCm39)	W284*	probably null	Het
Tlcd5	C	T	9: 43,023,080 (GRCm39)	W91*	probably null	Het
Tmem181a	A	G	17: 6,353,540 (GRCm39)	D384G	possibly damaging	Het
Txndc12	T	C	4: 108,713,368 (GRCm39)	S77P	probably damaging	Het
Wnt16	G	A	6: 22,288,560 (GRCm39)	V19M	probably benign	Het
Zfp959	G	T	17: 56,205,334 (GRCm39)	C457F	probably damaging	Het
Zscan4-ps3	T	C	7: 11,346,863 (GRCm39)	S300P	probably benign	Het

Other mutations in Zfp932

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0153:Zfp932	UTSW	5	110,154,834 (GRCm39)	missense	probably benign	0.27
R0268:Zfp932	UTSW	5	110,156,929 (GRCm39)	missense	probably benign	0.24
R1673:Zfp932	UTSW	5	110,156,854 (GRCm39)	missense	probably damaging	1.00
R1797:Zfp932	UTSW	5	110,144,489 (GRCm39)	start gained	probably benign
R1893:Zfp932	UTSW	5	110,157,069 (GRCm39)	missense	possibly damaging	0.71
R1942:Zfp932	UTSW	5	110,154,853 (GRCm39)	missense	probably damaging	1.00
R2073:Zfp932	UTSW	5	110,157,684 (GRCm39)	missense	possibly damaging	0.60
R2076:Zfp932	UTSW	5	110,157,334 (GRCm39)	missense	probably benign	0.01
R2329:Zfp932	UTSW	5	110,157,406 (GRCm39)	missense	probably benign	0.01
R3944:Zfp932	UTSW	5	110,157,820 (GRCm39)	missense	probably benign	0.37
R4551:Zfp932	UTSW	5	110,157,505 (GRCm39)	missense	probably benign	0.03
R4679:Zfp932	UTSW	5	110,157,760 (GRCm39)	missense	probably damaging	1.00
R4692:Zfp932	UTSW	5	110,157,052 (GRCm39)	missense	probably damaging	1.00
R5116:Zfp932	UTSW	5	110,157,242 (GRCm39)	missense	probably benign	0.05
R5260:Zfp932	UTSW	5	110,157,501 (GRCm39)	nonsense	probably null
R5536:Zfp932	UTSW	5	110,157,713 (GRCm39)	nonsense	probably null
R6351:Zfp932	UTSW	5	110,157,209 (GRCm39)	nonsense	probably null
R6786:Zfp932	UTSW	5	110,157,606 (GRCm39)	missense	probably damaging	0.98
R6843:Zfp932	UTSW	5	110,156,581 (GRCm39)	missense	probably benign	0.02
R7496:Zfp932	UTSW	5	110,156,694 (GRCm39)	missense	probably damaging	0.97
R7704:Zfp932	UTSW	5	110,157,630 (GRCm39)	missense	probably benign	0.09
R8224:Zfp932	UTSW	5	110,144,480 (GRCm39)	start gained	probably benign
R8317:Zfp932	UTSW	5	110,156,922 (GRCm39)	nonsense	probably null
R8765:Zfp932	UTSW	5	110,154,827 (GRCm39)	missense	probably benign	0.01
R8967:Zfp932	UTSW	5	110,156,883 (GRCm39)	missense	probably benign	0.00
R9083:Zfp932	UTSW	5	110,157,100 (GRCm39)	missense	probably damaging	1.00
R9142:Zfp932	UTSW	5	110,157,520 (GRCm39)	missense	probably damaging	1.00
R9511:Zfp932	UTSW	5	110,155,177 (GRCm39)	missense	possibly damaging	0.46
R9533:Zfp932	UTSW	5	110,157,787 (GRCm39)	missense	probably damaging	1.00
R9640:Zfp932	UTSW	5	110,157,064 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AAGCCTTTGCACGACACAGT -3'
(R):5'- TCTTGATCAGAAGCCAGAAGACTCT -3'

Sequencing Primer
(F):5'- CAATGTGGTAAAGCCCTTGC -3'
(R):5'- TGTGGATCTCAAGGTCCAAGC -3'

Posted On

2019-10-07