Mutagenetix > Incidental Mutation

Incidental Mutation 'R7440:Olfr362'

576867

Institutional Source

Beutler Lab

Gene Symbol

Olfr362

Ensembl Gene

ENSMUSG00000075377

Gene Name

olfactory receptor 362

Synonyms

MOR158-1, GA_x6K02T2NLDC-33797415-33796462

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R7440 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

37101706-37117910 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 37105169 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 160 (H160Q)

Ref Sequence

ENSEMBL: ENSMUSP00000150819 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100144] [ENSMUST00000213817] [ENSMUST00000215927]

AlphaFold

Q8VGV7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000100144
AA Change: H160Q

PolyPhen 2 Score 0.850 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000097722
Gene: ENSMUSG00000075377
AA Change: H160Q

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	313	6.3e-50	PFAM
Pfam:7TM_GPCR_Srsx	36	164	1.6e-7	PFAM
Pfam:7tm_1	42	295	1.3e-23	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000213817
AA Change: H160Q

PolyPhen 2 Score 0.850 (Sensitivity: 0.83; Specificity: 0.93)

Predicted Effect

possibly damaging
Transcript: ENSMUST00000215927
AA Change: H160Q

PolyPhen 2 Score 0.850 (Sensitivity: 0.83; Specificity: 0.93)

Meta Mutation Damage Score

0.1712

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (69/69)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110032A03Rik	A	G	9: 50,764,913	V35A	probably benign	Het
9230104L09Rik	A	C	2: 148,846,991	C109W	probably damaging	Het
Acadm	G	A	3: 153,922,989	T403I	probably damaging	Het
Adat1	A	T	8: 111,989,898	M64K	probably damaging	Het
Adcy2	C	T	13: 68,796,667	V199M	probably damaging	Het
Ankrd13a	T	C	5: 114,803,575	S508P	possibly damaging	Het
Ap1g1	T	A	8: 109,802,724		probably null	Het
Bicral	C	T	17: 46,825,784	G167R	probably damaging	Het
Ccser2	T	C	14: 36,898,217	K727E	possibly damaging	Het
Cep350	T	G	1: 155,940,772	K332N	probably damaging	Het
Chd5	A	G	4: 152,384,651	N1811S	probably benign	Het
Chpf	A	T	1: 75,475,601	V565D	probably damaging	Het
Clcn6	A	T	4: 148,014,195	L489H	probably damaging	Het
Cntln	C	A	4: 85,063,216	T877K	possibly damaging	Het
Cog4	T	C	8: 110,879,706	V630A	probably benign	Het
Col6a5	G	T	9: 105,881,431	S2192*	probably null	Het
Cry2	G	A	2: 92,413,638	R397W	probably damaging	Het
Cyp1b1	T	C	17: 79,713,557	N252S	probably damaging	Het
Dhx9	T	C	1: 153,481,231	I91V	probably benign	Het
Dnajb6	G	A	5: 29,757,859	A256T	possibly damaging	Het
Epm2a	T	G	10: 11,390,875	Y121*	probably null	Het
Erlec1	T	C	11: 30,950,818	I117V	possibly damaging	Het
Exoc8	T	C	8: 124,895,781	M616V	probably benign	Het
Fmn1	A	T	2: 113,441,611	Q108L	unknown	Het
Fuz	T	C	7: 44,896,572	L46P	probably damaging	Het
Gm15319	C	T	8: 20,356,948	S254N	unknown	Het
Insc	T	C	7: 114,845,043	S422P	possibly damaging	Het
Intu	A	G	3: 40,697,551	I813V	probably benign	Het
Jak3	T	A	8: 71,680,718	S352T	probably benign	Het
Klf11	T	C	12: 24,655,491	S315P	probably benign	Het
Lrrc63	T	C	14: 75,121,013	N400D	possibly damaging	Het
Lrrk1	T	C	7: 66,290,854	D760G	probably damaging	Het
Macf1	A	T	4: 123,455,446	L3976*	probably null	Het
Map7	C	T	10: 20,261,859	A259V	probably damaging	Het
Meioc	T	A	11: 102,674,237	D170E	possibly damaging	Het
Mgat5b	T	A	11: 116,968,445	Y34*	probably null	Het
Mgst1	A	G	6: 138,150,844	K68R	probably benign	Het
Miga1	T	A	3: 152,338,046		probably null	Het
Mrps33	G	A	6: 39,802,479	P94L	probably damaging	Het
Ncapg2	G	T	12: 116,450,413	G1068C	possibly damaging	Het
Ndufaf7	C	T	17: 78,942,117	H148Y	probably damaging	Het
Nkx6-3	A	T	8: 23,153,754	D57V	probably damaging	Het
Nlrp1a	T	C	11: 71,092,324	D1272G	probably damaging	Het
Oit3	T	C	10: 59,429,570	N291S	probably damaging	Het
Pld1	T	G	3: 28,041,270	S251A	probably benign	Het
Ppfibp1	T	C	6: 147,019,503	S580P	probably benign	Het
Prdx6b	A	T	2: 80,293,216	D123V	probably damaging	Het
Ptprs	A	G	17: 56,424,256	L1050P	possibly damaging	Het
Rcc1	A	C	4: 132,337,799	S138A	probably damaging	Het
Rimbp3	G	T	16: 17,213,201	R1496S	possibly damaging	Het
Sacs	T	C	14: 61,191,605	V371A	probably benign	Het
Sfxn4	A	G	19: 60,842,204	L260P	possibly damaging	Het
Slc17a1	G	A	13: 23,878,483	S211N	possibly damaging	Het
Slc39a11	C	T	11: 113,562,092	V8M	probably damaging	Het
Smpd2	A	G	10: 41,489,016	I78T	probably benign	Het
Steap3	A	C	1: 120,241,518	F350V	probably benign	Het
Syt17	A	T	7: 118,381,884	V462E	probably damaging	Het
Tlr11	T	A	14: 50,361,344	D262E	probably benign	Het
Tnfrsf9	T	C	4: 150,929,874	V10A	probably benign	Het
Trpv3	C	A	11: 73,277,974	Q87K	probably benign	Het
Ugt1a5	T	C	1: 88,166,559	Y170H	probably benign	Het
Urb1	A	G	16: 90,787,408	L562P	probably damaging	Het
Usp3	G	T	9: 66,530,255	N299K	probably benign	Het
Vmn1r83	T	C	7: 12,321,629	Y167C	probably damaging	Het
Vmn2r117	T	A	17: 23,475,565	Y436F	probably benign	Het
Vps13d	A	G	4: 145,128,411	I2220T		Het
Zfp936	T	A	7: 43,187,261	V32D	probably damaging	Het
Znrd1	T	A	17: 36,957,844	L75F	probably benign	Het
Zswim2	A	G	2: 83,920,719	C259R	probably damaging	Het

Other mutations in Olfr362

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02032:Olfr362	APN	2	37104761	missense	probably damaging	1.00
IGL02141:Olfr362	APN	2	37105425	missense	probably benign	0.00
IGL02444:Olfr362	APN	2	37104774	missense	probably damaging	0.99
IGL02453:Olfr362	APN	2	37105197	missense	probably benign	0.22
R0699:Olfr362	UTSW	2	37105062	missense	possibly damaging	0.92
R1053:Olfr362	UTSW	2	37105464	missense	probably damaging	1.00
R1387:Olfr362	UTSW	2	37104868	missense	probably benign	0.24
R4914:Olfr362	UTSW	2	37105158	missense	possibly damaging	0.67
R4918:Olfr362	UTSW	2	37105158	missense	possibly damaging	0.67
R5009:Olfr362	UTSW	2	37105455	missense	possibly damaging	0.92
R5114:Olfr362	UTSW	2	37104802	missense	probably damaging	1.00
R5301:Olfr362	UTSW	2	37105198	missense	probably benign	0.01
R7322:Olfr362	UTSW	2	37105591	missense	probably null	0.00
R7583:Olfr362	UTSW	2	37105527	nonsense	probably null
R8892:Olfr362	UTSW	2	37105511	missense	probably damaging	1.00
R9264:Olfr362	UTSW	2	37104789	missense	probably damaging	1.00
Z1176:Olfr362	UTSW	2	37105636	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- GGGAACAAGGTCCCAACATTAG -3'
(R):5'- ACAGCTGTTGGTTCATCTGTC -3'

Sequencing Primer
(F):5'- GGAACAAGGTCCCAACATTAGAAAGC -3'
(R):5'- GCCCGCTGTTTGACTCAG -3'

Posted On

2019-10-07