Mutagenetix > Incidental Mutations

Incidental Mutation 'R7441:Zfp810'

576957

Institutional Source

Beutler Lab

Gene Symbol

Zfp810

Ensembl Gene

ENSMUSG00000066829

Gene Name

zinc finger protein 810

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R7441 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

22276748-22307648 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 22279272 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 78 (E78*)

Ref Sequence

ENSEMBL: ENSMUSP00000150816 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086278] [ENSMUST00000215202]

AlphaFold

Q99K45

Predicted Effect

silent
Transcript: ENSMUST00000086278

SMART Domains

Protein: ENSMUSP00000083459
Gene: ENSMUSG00000066829

Domain	Start	End	E-Value	Type
KRAB	4	64	1.09e-33	SMART
ZnF_C2H2	126	148	2.44e2	SMART
ZnF_C2H2	182	204	3.07e-1	SMART
ZnF_C2H2	210	232	8.47e-4	SMART
ZnF_C2H2	238	260	6.78e-3	SMART
ZnF_C2H2	266	288	6.13e-1	SMART
ZnF_C2H2	294	316	5.06e-2	SMART
ZnF_C2H2	322	344	4.79e-3	SMART
ZnF_C2H2	350	372	2.99e-4	SMART
ZnF_C2H2	378	400	1.33e-1	SMART
ZnF_C2H2	406	428	2.75e-3	SMART
ZnF_C2H2	434	456	1.58e-3	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000215202
AA Change: E78*

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700007K13Rik	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	TCTCTGGGGCAGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTCTGGGGCGGGCTTAGCCTTGGGCTCCCCCGGCTCCGGCTCCTC	2: 28,466,110		probably benign	Het
4921509C19Rik	A	C	2: 151,472,925	S278A	possibly damaging	Het
Adamts20	T	C	15: 94,353,673	D411G	probably damaging	Het
Adgrl3	A	G	5: 81,724,140	I894V	possibly damaging	Het
Adprhl1	C	T	8: 13,223,069	V1230I	probably benign	Het
Agpat1	T	A	17: 34,610,909	Y77N	probably damaging	Het
Anpep	A	G	7: 79,827,644	V725A	possibly damaging	Het
Apc	T	A	18: 34,312,073	I674K	probably damaging	Het
Arhgef2	A	G	3: 88,643,955	R808G	probably damaging	Het
Asap1	A	G	15: 64,130,256	V402A	probably damaging	Het
Aspg	T	C	12: 112,124,821	V479A	possibly damaging	Het
B3galnt2	G	A	13: 13,994,485	V368M	probably benign	Het
Bahcc1	T	C	11: 120,286,306	S2007P	probably damaging	Het
Cyfip2	A	T	11: 46,196,427	I1212N	possibly damaging	Het
Dnajc16	A	T	4: 141,763,813	D675E	probably damaging	Het
Dram2	T	C	3: 106,555,187	F4L	probably damaging	Het
Dsp	A	G	13: 38,195,449	T2057A	probably benign	Het
Dync1h1	T	G	12: 110,636,453	L2176R	probably damaging	Het
Efhb	A	T	17: 53,401,521	I707N	possibly damaging	Het
Eif2ak4	A	G	2: 118,471,896	T1555A	probably benign	Het
Erc1	G	A	6: 119,824,951	T35I	possibly damaging	Het
Esr2	C	A	12: 76,141,394	M363I	probably benign	Het
Etl4	A	T	2: 20,744,189	N446I	possibly damaging	Het
Evpl	T	A	11: 116,222,956	K1303*	probably null	Het
Fam129c	A	G	8: 71,600,164	D94G	probably benign	Het
Fam135b	A	T	15: 71,463,680	V555E	probably damaging	Het
Fam186b	A	G	15: 99,280,089	L452P	probably benign	Het
Fmn1	A	T	2: 113,441,611	Q108L	unknown	Het
Gcc2	A	G	10: 58,256,901	T48A	probably benign	Het
Gm12169	G	A	11: 46,528,555	W66*	probably null	Het
Gm6619	T	G	6: 131,490,391	I73S	possibly damaging	Het
Gm8267	T	A	14: 44,722,940	D116V	probably damaging	Het
Gtf3c3	G	A	1: 54,420,448	T385M	probably benign	Het
Iqgap2	C	A	13: 95,628,076	M1553I	probably benign	Het
Kcnk1	G	T	8: 125,995,568	G37C	probably damaging	Het
Kifc3	T	C	8: 95,137,987	M32V	probably benign	Het
Krt81	C	A	15: 101,461,370	K222N	possibly damaging	Het
Lrrc63	A	G	14: 75,126,257	S145P	possibly damaging	Het
Mybbp1a	T	A	11: 72,451,275	V1279E	probably benign	Het
Olfr1044	T	A	2: 86,171,010	D269V	probably damaging	Het
Olfr742	A	T	14: 50,515,396	Y64F	probably damaging	Het
Pramef8	A	G	4: 143,418,840	Y293C	probably benign	Het
Ptpn18	T	C	1: 34,473,335	V407A	probably benign	Het
Ptpn9	T	A	9: 57,027,433	Y160*	probably null	Het
Ptprj	T	C	2: 90,449,819	K1045R	possibly damaging	Het
Rundc3a	G	T	11: 102,400,046		probably null	Het
Scn11a	C	T	9: 119,758,626	V1351I	probably benign	Het
Slc26a9	A	G	1: 131,762,818	Y520C	probably damaging	Het
Spata20	G	A	11: 94,484,041	A245V	probably benign	Het
Steap3	A	C	1: 120,241,518	F350V	probably benign	Het
Swt1	A	T	1: 151,411,064	F226I	probably benign	Het
Taar7f	A	G	10: 24,049,987	T160A	possibly damaging	Het
Upf2	A	T	2: 6,018,932	I698F	unknown	Het
Vmn2r84	T	C	10: 130,392,113	T85A	possibly damaging	Het
Zfp426	T	C	9: 20,470,851	E280G	possibly damaging	Het
Zfp937	GTGATAAGGCATTTGCACAAAACAGTCATCTCCTAACACATAAAAGAACACAT	G	2: 150,238,710		probably null	Het

Other mutations in Zfp810

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00484:Zfp810	APN	9	22278309	nonsense	probably null
IGL03079:Zfp810	APN	9	22284127	missense	probably damaging	1.00
IGL03402:Zfp810	APN	9	22279145	splice site	probably null
H8562:Zfp810	UTSW	9	22279091	missense	probably benign	0.42
R1116:Zfp810	UTSW	9	22279085	missense	probably benign	0.11
R1160:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R1171:Zfp810	UTSW	9	22278826	missense	possibly damaging	0.95
R1393:Zfp810	UTSW	9	22280514	missense	probably benign
R1608:Zfp810	UTSW	9	22278920	missense	probably benign	0.00
R1644:Zfp810	UTSW	9	22279028	missense	possibly damaging	0.67
R1766:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R2568:Zfp810	UTSW	9	22279238	missense	probably benign	0.01
R3684:Zfp810	UTSW	9	22278235	missense	probably benign	0.01
R4002:Zfp810	UTSW	9	22278892	missense	probably damaging	1.00
R4134:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4135:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4334:Zfp810	UTSW	9	22278784	missense	probably benign	0.00
R4545:Zfp810	UTSW	9	22278745	missense	probably damaging	0.96
R5399:Zfp810	UTSW	9	22278829	missense	possibly damaging	0.91
R5622:Zfp810	UTSW	9	22279096	missense	probably benign	0.00
R5643:Zfp810	UTSW	9	22283171	missense	probably benign	0.26
R7375:Zfp810	UTSW	9	22290537	critical splice donor site	probably null
R7809:Zfp810	UTSW	9	22278982	missense	possibly damaging	0.51
R8422:Zfp810	UTSW	9	22283222	nonsense	probably null
R8526:Zfp810	UTSW	9	22278290	missense	probably damaging	1.00
R8719:Zfp810	UTSW	9	22279275	missense	probably benign	0.00
R9177:Zfp810	UTSW	9	22278640	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCCTCTGATACTGACTGAGGG -3'
(R):5'- GCAGATCAGGAAGCAGAGTTCC -3'

Sequencing Primer
(F):5'- CCTCTGATACTGACTGAGGGTAGAC -3'
(R):5'- TCAGGAAGCAGAGTTCCTAATAC -3'

Posted On

2019-10-07