|Institutional Source||Beutler Lab|
|Gene Name||tribbles pseudokinase 3|
|Synonyms||Nipk, Ifld2, Trb3, SKIP3|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R7443 (G1)|
|Chromosomal Location||152337422-152344032 bp(-) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 152339772 bp|
|Amino Acid Change||Histidine to Arginine at position 176 (H176R)|
|Ref Sequence||ENSEMBL: ENSMUSP00000041747 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000040312]|
|Predicted Effect||possibly damaging
AA Change: H176R
PolyPhen 2 Score 0.899 (Sensitivity: 0.82; Specificity: 0.94)
AA Change: H176R
|Coding Region Coverage||
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a putative protein kinase that is induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. In addition, this protein can negatively regulate the cell survival serine-threonine kinase AKT1. Differential promoter usage and alternate splicing result in multiple transcript variants. [provided by RefSeq, Jul 2014]
PHENOTYPE: Homozygous null mutants may display hypoactivity, decreased blood pressure and abnormal digit morphology or abnormal mast cell physiology. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Trib3||
(F):5'- GAGCCTTGCACATGCTAAGC -3'
(R):5'- TGACTCTGTCTCCAGGTGTAC -3'
(F):5'- TACCTCTCAAGTGTGCCT -3'
(R):5'- TGCCTACCCACCAGCATGTG -3'