Mutagenetix > Incidental Mutation

Incidental Mutation 'R0629:Mroh9'

57733

Institutional Source

Beutler Lab

Gene Symbol

Mroh9

Ensembl Gene

ENSMUSG00000071890

Gene Name

maestro heat-like repeat family member 9

Synonyms

4921528O07Rik, Armc11

MMRRC Submission

038818-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0629 (G1)

Quality Score

214

Status

Validated

Chromosome

Chromosomal Location

163024302-163085670 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 163060636 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Arginine at position 290 (H290R)

Ref Sequence

ENSEMBL: ENSMUSP00000094365 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096608]

AlphaFold

G5E8L9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000096608
AA Change: H290R

PolyPhen 2 Score 0.905 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000094365
Gene: ENSMUSG00000071890
AA Change: H290R

Domain	Start	End	E-Value	Type
SCOP:d1gw5b_	231	716	2e-8	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000195132

Meta Mutation Damage Score

0.3092

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.6%
20x: 95.6%

Validation Efficiency

100% (66/66)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars2	A	T	17: 45,507,547	D86V	probably damaging	Het
Adamts14	G	A	10: 61,211,624	Q733*	probably null	Het
Adcy10	A	G	1: 165,543,105	D651G	probably damaging	Het
Apcdd1	T	A	18: 62,933,970	C52S	probably damaging	Het
Bclaf1	T	C	10: 20,333,426	S463P	probably damaging	Het
Cabcoco1	T	C	10: 68,516,278	Y68C	probably damaging	Het
Cacna1f	G	A	X: 7,620,434	S888N	probably damaging	Het
Cacna1g	A	G	11: 94,409,543	C2134R	possibly damaging	Het
Cdc37	A	C	9: 21,140,768	M325R	possibly damaging	Het
Clca2	T	A	3: 145,072,239	M762L	probably benign	Het
Cntn3	C	T	6: 102,203,976	V753M	probably damaging	Het
Col6a6	A	T	9: 105,727,165		probably benign	Het
Dscaml1	A	G	9: 45,721,418	D1194G	probably damaging	Het
Egfr	G	A	11: 16,869,333	G288S	probably damaging	Het
Fbxl17	G	T	17: 63,471,414	N19K	probably damaging	Het
Fmo3	A	G	1: 162,958,227		probably benign	Het
Frmd6	T	C	12: 70,883,762	Y219H	probably damaging	Het
Fuca1	T	C	4: 135,925,644	V193A	possibly damaging	Het
Gm1141	G	C	X: 71,938,773	R296P	possibly damaging	Het
Gm7461	C	T	8: 4,677,769		noncoding transcript	Het
Gpc5	T	A	14: 115,552,239	N508K	possibly damaging	Het
Iqch	A	T	9: 63,425,382	D1019E	probably benign	Het
Isyna1	A	G	8: 70,594,708	Y27C	probably damaging	Het
Itgb8	T	G	12: 119,202,481	H105P	probably benign	Het
Kbtbd11	C	T	8: 15,027,572	P57L	probably benign	Het
Kcns3	A	C	12: 11,092,558	C47G	probably damaging	Het
Kif21b	A	T	1: 136,172,157		probably null	Het
Lama3	A	T	18: 12,419,245	H418L	possibly damaging	Het
Lrit3	A	G	3: 129,788,302	Y679H	probably damaging	Het
Lrrc19	T	A	4: 94,638,252	D356V	probably damaging	Het
Morc2b	A	G	17: 33,135,807	M997T	probably benign	Het
Mtcl1	A	T	17: 66,338,142	S1886T	possibly damaging	Het
Muc20	T	C	16: 32,793,421	T529A	possibly damaging	Het
Myo7a	A	C	7: 98,085,466	L607R	probably damaging	Het
Myom2	T	A	8: 15,069,783	F180I	probably damaging	Het
Myt1l	G	A	12: 29,811,485	E89K	unknown	Het
Nek2	A	G	1: 191,831,317	N431S	probably benign	Het
Olfr1086	T	A	2: 86,676,529	H268L	possibly damaging	Het
Olfr169	A	T	16: 19,565,980	V301E	possibly damaging	Het
Oprm1	A	T	10: 6,832,604		probably null	Het
Oxsr1	A	G	9: 119,241,784		probably benign	Het
Pdgfrb	G	A	18: 61,078,648		probably null	Het
Proser1	C	A	3: 53,479,064	P789Q	probably benign	Het
Ptgs2	A	G	1: 150,101,037	Q7R	probably benign	Het
Rab3d	A	G	9: 21,914,686	V144A	probably benign	Het
Ralgapb	T	A	2: 158,439,547	L167H	probably damaging	Het
Ranbp3	A	G	17: 56,708,200	T301A	possibly damaging	Het
Rasgrf1	G	A	9: 89,984,269	V587M	probably damaging	Het
Sec16b	A	G	1: 157,564,863		probably benign	Het
Sin3b	T	C	8: 72,753,536		probably benign	Het
Slc10a2	T	C	8: 5,098,562	S128G	probably benign	Het
Tbl1xr1	G	A	3: 22,210,401	V507I	probably benign	Het
Tmem8b	T	G	4: 43,669,896		probably null	Het
Trak1	A	T	9: 121,367,167	T22S	probably benign	Het
Trim30d	A	G	7: 104,487,655	I114T	probably damaging	Het
Ttc13	A	T	8: 124,674,366	S624T	probably damaging	Het
Ttn	T	C	2: 76,828,130		probably benign	Het
Vipr1	T	A	9: 121,660,171	Y99*	probably null	Het
Vmn1r210	T	C	13: 22,827,874	K81E	probably damaging	Het
Wwc1	T	C	11: 35,853,472	Y841C	probably benign	Het
Xrcc4	A	G	13: 90,000,905		probably benign	Het
Zdhhc22	A	T	12: 86,988,297	I127N	probably damaging	Het
Zdhhc7	A	G	8: 120,088,046	L8P	possibly damaging	Het
Zfp664	C	A	5: 124,885,595	L18I	probably damaging	Het

Other mutations in Mroh9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Mroh9	APN	1	163045781	missense	possibly damaging	0.89
IGL00705:Mroh9	APN	1	163079503	missense	probably damaging	1.00
IGL00788:Mroh9	APN	1	163024658	missense	probably benign	0.06
IGL00795:Mroh9	APN	1	163060622	missense	probably damaging	1.00
IGL00815:Mroh9	APN	1	163039131	missense	probably damaging	1.00
IGL01025:Mroh9	APN	1	163047866	missense	possibly damaging	0.67
IGL01303:Mroh9	APN	1	163080575	missense	probably benign	0.00
IGL01526:Mroh9	APN	1	163055603	missense	probably damaging	0.99
IGL01680:Mroh9	APN	1	163047982	splice site	probably null
IGL01823:Mroh9	APN	1	163055609	missense	probably benign	0.39
IGL02024:Mroh9	APN	1	163062502	missense	possibly damaging	0.65
IGL02213:Mroh9	APN	1	163058079	missense	probably damaging	1.00
IGL02455:Mroh9	APN	1	163075580	missense	probably benign	0.03
IGL02546:Mroh9	APN	1	163080576	missense	probably benign	0.04
IGL03059:Mroh9	APN	1	163024636	missense	possibly damaging	0.95
IGL03061:Mroh9	APN	1	163026502	missense	probably damaging	1.00
IGL03071:Mroh9	APN	1	163039197	missense	probably damaging	1.00
R0048:Mroh9	UTSW	1	163062487	missense	probably damaging	0.97
R0048:Mroh9	UTSW	1	163062487	missense	probably damaging	0.97
R0441:Mroh9	UTSW	1	163060762	missense	probably damaging	1.00
R0506:Mroh9	UTSW	1	163060636	missense	possibly damaging	0.90
R0751:Mroh9	UTSW	1	163066124	missense	possibly damaging	0.84
R1301:Mroh9	UTSW	1	163043983	critical splice donor site	probably null
R1481:Mroh9	UTSW	1	163026509	missense	probably damaging	1.00
R1618:Mroh9	UTSW	1	163024541	missense	probably benign	0.00
R1647:Mroh9	UTSW	1	163046056	missense	probably damaging	1.00
R1648:Mroh9	UTSW	1	163046056	missense	probably damaging	1.00
R1668:Mroh9	UTSW	1	163024592	missense	possibly damaging	0.52
R1795:Mroh9	UTSW	1	163056778	missense	probably damaging	0.97
R1796:Mroh9	UTSW	1	163045710	missense	probably damaging	1.00
R1857:Mroh9	UTSW	1	163039145	missense	probably damaging	0.98
R1869:Mroh9	UTSW	1	163026513	missense	probably damaging	0.97
R1923:Mroh9	UTSW	1	163076291	missense	probably damaging	1.00
R2325:Mroh9	UTSW	1	163026530	splice site	probably null
R2511:Mroh9	UTSW	1	163038945	missense	probably benign	0.13
R2912:Mroh9	UTSW	1	163044003	missense	probably damaging	1.00
R2913:Mroh9	UTSW	1	163044003	missense	probably damaging	1.00
R2919:Mroh9	UTSW	1	163056772	missense	probably damaging	1.00
R2973:Mroh9	UTSW	1	163056769	missense	probably damaging	1.00
R3912:Mroh9	UTSW	1	163066069	missense	probably damaging	0.97
R4034:Mroh9	UTSW	1	163080553	critical splice donor site	probably null
R4551:Mroh9	UTSW	1	163044093	missense	probably damaging	0.98
R4656:Mroh9	UTSW	1	163066024	missense	probably damaging	1.00
R4662:Mroh9	UTSW	1	163055593	missense	probably damaging	0.97
R4743:Mroh9	UTSW	1	163024492	missense	probably benign	0.05
R4890:Mroh9	UTSW	1	163026524	missense	probably damaging	1.00
R5128:Mroh9	UTSW	1	163060760	missense	probably damaging	1.00
R5129:Mroh9	UTSW	1	163060760	missense	probably damaging	1.00
R5147:Mroh9	UTSW	1	163060760	missense	probably damaging	1.00
R5157:Mroh9	UTSW	1	163044121	missense	probably damaging	0.96
R5324:Mroh9	UTSW	1	163060760	missense	probably damaging	1.00
R5325:Mroh9	UTSW	1	163060760	missense	probably damaging	1.00
R5340:Mroh9	UTSW	1	163080587	start gained	probably benign
R6005:Mroh9	UTSW	1	163075677	missense	probably damaging	0.99
R6182:Mroh9	UTSW	1	163066043	nonsense	probably null
R6414:Mroh9	UTSW	1	163074702	missense	probably damaging	1.00
R6477:Mroh9	UTSW	1	163076304	missense	probably damaging	1.00
R6540:Mroh9	UTSW	1	163038972	missense	possibly damaging	0.87
R6541:Mroh9	UTSW	1	163058038	missense	possibly damaging	0.78
R6643:Mroh9	UTSW	1	163075561	missense	probably damaging	1.00
R6811:Mroh9	UTSW	1	163046041	missense	possibly damaging	0.86
R6830:Mroh9	UTSW	1	163076366	missense	probably benign
R7026:Mroh9	UTSW	1	163060682	missense	probably benign	0.00
R7052:Mroh9	UTSW	1	163038956	missense	possibly damaging	0.92
R7068:Mroh9	UTSW	1	163039181	missense	probably damaging	1.00
R7350:Mroh9	UTSW	1	163076289	critical splice donor site	probably null
R7545:Mroh9	UTSW	1	163074708	missense	possibly damaging	0.56
R7615:Mroh9	UTSW	1	163046032	missense	probably benign	0.40
R7743:Mroh9	UTSW	1	163024553	missense	probably benign
R7808:Mroh9	UTSW	1	163039109	missense	probably damaging	1.00
R8024:Mroh9	UTSW	1	163039233	missense	probably benign	0.02
R8062:Mroh9	UTSW	1	163038975	missense	probably damaging	1.00
R8145:Mroh9	UTSW	1	163062527	missense	probably benign	0.00
R8426:Mroh9	UTSW	1	163024725	missense	probably damaging	0.98
R8458:Mroh9	UTSW	1	163055681	missense	probably damaging	1.00
R8555:Mroh9	UTSW	1	163072026	splice site	probably null
R8960:Mroh9	UTSW	1	163055627	missense	probably benign	0.25
R9040:Mroh9	UTSW	1	163062500	missense	probably benign	0.06
R9125:Mroh9	UTSW	1	163047843	missense	probably benign	0.19
R9154:Mroh9	UTSW	1	163062461	missense
R9596:Mroh9	UTSW	1	163066007	missense	probably damaging	0.98
R9612:Mroh9	UTSW	1	163038929	missense	probably damaging	1.00
RF003:Mroh9	UTSW	1	163058061	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGTGCAACTTCAAGCTGAGAGTCC -3'
(R):5'- TGTCCAGAGCATCCCCTAAAGTGTC -3'

Sequencing Primer
(F):5'- CTTCAAGCTGAGAGTCCTTGAAAATG -3'
(R):5'- GCATCCCCTAAAGTGTCTAAGATTTG -3'

Posted On

2013-07-11