Mutagenetix > Incidental Mutation

Incidental Mutation 'R7448:Cobl'

577491

Institutional Source

Beutler Lab

Gene Symbol

Cobl

Ensembl Gene

ENSMUSG00000020173

Gene Name

cordon-bleu WH2 repeat

Synonyms

C530045F18Rik

MMRRC Submission

045523-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7448 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

12186676-12415022 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 12206225 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Arginine at position 550 (M550R)

Ref Sequence

ENSEMBL: ENSMUSP00000045693 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046755] [ENSMUST00000109650] [ENSMUST00000109651] [ENSMUST00000172919] [ENSMUST00000172956] [ENSMUST00000174874]

AlphaFold

Q5NBX1

PDB Structure

Actin complex with Gelsolin Segment 1 fused to Cobl segment [X-RAY DIFFRACTION]
Crystal Structure of an Actin Dimer in Complex with the Actin Nucleator Cordon-Bleu [X-RAY DIFFRACTION]

Predicted Effect

possibly damaging
Transcript: ENSMUST00000046755
AA Change: M550R

PolyPhen 2 Score 0.458 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000045693
Gene: ENSMUSG00000020173
AA Change: M550R

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	144	235	2.2e-46	PFAM
low complexity region	328	333	N/A	INTRINSIC
low complexity region	360	376	N/A	INTRINSIC
low complexity region	408	433	N/A	INTRINSIC
low complexity region	468	482	N/A	INTRINSIC
low complexity region	526	541	N/A	INTRINSIC
coiled coil region	564	589	N/A	INTRINSIC
WH2	1185	1205	1.32e0	SMART
WH2	1225	1245	6.36e-3	SMART
low complexity region	1276	1296	N/A	INTRINSIC
WH2	1313	1333	3.91e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109650
AA Change: M468R

PolyPhen 2 Score 0.405 (Sensitivity: 0.89; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000105277
Gene: ENSMUSG00000020173
AA Change: M468R

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	182	260	1.6e-40	PFAM
low complexity region	303	308	N/A	INTRINSIC
low complexity region	335	351	N/A	INTRINSIC
low complexity region	386	400	N/A	INTRINSIC
low complexity region	444	459	N/A	INTRINSIC
coiled coil region	482	507	N/A	INTRINSIC
WH2	1103	1123	1.32e0	SMART
WH2	1143	1163	6.36e-3	SMART
low complexity region	1194	1214	N/A	INTRINSIC
WH2	1231	1251	3.91e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000109651
AA Change: M525R

PolyPhen 2 Score 0.177 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000105278
Gene: ENSMUSG00000020173
AA Change: M525R

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	182	260	1.2e-40	PFAM
low complexity region	303	308	N/A	INTRINSIC
low complexity region	335	351	N/A	INTRINSIC
low complexity region	383	408	N/A	INTRINSIC
low complexity region	443	457	N/A	INTRINSIC
low complexity region	501	516	N/A	INTRINSIC
coiled coil region	539	564	N/A	INTRINSIC
WH2	1160	1180	1.32e0	SMART
WH2	1200	1220	6.36e-3	SMART
low complexity region	1251	1271	N/A	INTRINSIC
WH2	1288	1308	3.91e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000172919

SMART Domains

Protein: ENSMUSP00000133669
Gene: ENSMUSG00000020173

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	182	260	2.6e-41	PFAM
low complexity region	328	333	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000172956
AA Change: C372G

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000134372
Gene: ENSMUSG00000020173
AA Change: C372G

Domain	Start	End	E-Value	Type
low complexity region	14	30	N/A	INTRINSIC
Pfam:Cobl	182	260	2.4e-41	PFAM
low complexity region	303	308	N/A	INTRINSIC
low complexity region	335	351	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000174874
AA Change: M543R

PolyPhen 2 Score 0.458 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000133470
Gene: ENSMUSG00000020173
AA Change: M543R

Domain	Start	End	E-Value	Type
low complexity region	6	23	N/A	INTRINSIC
Pfam:Cobl	175	253	1.2e-40	PFAM
low complexity region	321	326	N/A	INTRINSIC
low complexity region	353	369	N/A	INTRINSIC
low complexity region	401	426	N/A	INTRINSIC
low complexity region	461	475	N/A	INTRINSIC
low complexity region	519	534	N/A	INTRINSIC
coiled coil region	557	582	N/A	INTRINSIC
WH2	1178	1198	1.32e0	SMART
WH2	1218	1238	6.36e-3	SMART
low complexity region	1269	1289	N/A	INTRINSIC
WH2	1306	1326	3.91e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (117/117)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that contains WH2 domains (WASP, Wiskott-Aldrich syndrome protein, homology domain-2) that interact with actin. The encoded actin regulator protein is required for growth and assembly of brush border microvilli that play a role in maintaining intestinal homeostasis. A similar protein in mouse functions in midbrain neural tube closure. A pseudogene of this gene is located on chromosome X. [provided by RefSeq, Oct 2016]
PHENOTYPE: Animals homozygous for this mutation do not display a phenotype. However, the allele exacerbates the neural tube defects seen in the loop tail mouse. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 119 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acss2	T	C	2: 155,360,186 (GRCm39)	S54P	probably damaging	Het
Ahnak2	T	C	12: 112,746,605 (GRCm39)	K1242E		Het
Alpi	T	A	1: 87,029,257 (GRCm39)	M1L	possibly damaging	Het
Atp2c1	C	T	9: 105,329,982 (GRCm39)	A283T	probably damaging	Het
Atp8b5	T	G	4: 43,366,021 (GRCm39)	M764R	probably benign	Het
B4galnt2	T	A	11: 95,760,193 (GRCm39)	H278L	probably damaging	Het
Bcl9l	G	T	9: 44,420,634 (GRCm39)	A1347S	probably benign	Het
Bicd2	A	G	13: 49,533,427 (GRCm39)	E671G	probably damaging	Het
Bmp3	A	T	5: 99,020,077 (GRCm39)	I167F	probably damaging	Het
Bpifb5	T	A	2: 154,072,105 (GRCm39)	C271S	possibly damaging	Het
Camsap2	T	A	1: 136,198,644 (GRCm39)	H793L		Het
Casp8ap2	T	A	4: 32,643,974 (GRCm39)	S1016T	possibly damaging	Het
Castor1	A	G	11: 4,171,897 (GRCm39)	S325G	not run	Het
Ccdc134	T	A	15: 82,025,149 (GRCm39)	I216N	possibly damaging	Het
Ccdc63	G	T	5: 122,246,245 (GRCm39)	R559S	probably benign	Het
Cd276	T	C	9: 58,442,895 (GRCm39)	T187A	probably benign	Het
Ciao1	C	T	2: 127,087,678 (GRCm39)	R219H	probably damaging	Het
Clmn	T	C	12: 104,751,687 (GRCm39)	D256G	possibly damaging	Het
Cracr2b	A	T	7: 141,044,118 (GRCm39)	T117S	probably benign	Het
Cx3cr1	G	A	9: 119,881,282 (GRCm39)	A40V	probably benign	Het
Cxcl14	A	G	13: 56,440,344 (GRCm39)	C72R	probably damaging	Het
Dab2	T	A	15: 6,451,747 (GRCm39)	I121N	probably damaging	Het
Dapk1	A	G	13: 60,898,990 (GRCm39)	Y820C	probably damaging	Het
Dele1	A	G	18: 38,390,319 (GRCm39)	N256D	probably damaging	Het
Ech1	T	A	7: 28,525,623 (GRCm39)	C91S	probably damaging	Het
Exosc5	T	G	7: 25,358,734 (GRCm39)	V25G	probably benign	Het
Fbp1	A	C	13: 63,020,564 (GRCm39)	D122E	possibly damaging	Het
Fbxw13	A	G	9: 109,014,471 (GRCm39)	Y101H	unknown	Het
Fcsk	C	T	8: 111,616,963 (GRCm39)	G396S	possibly damaging	Het
Fhip1a	G	A	3: 85,579,871 (GRCm39)	S778L	probably benign	Het
Fmnl1	T	A	11: 103,077,453 (GRCm39)	V271E	probably damaging	Het
Galnt1	T	A	18: 24,417,866 (GRCm39)	S545T	probably benign	Het
Galnt13	G	A	2: 54,406,576 (GRCm39)	V9M	possibly damaging	Het
Gm7995	A	T	14: 42,132,302 (GRCm39)	I45F		Het
Gpr137	C	T	19: 6,917,726 (GRCm39)	R134Q	possibly damaging	Het
Gpr22	T	G	12: 31,759,514 (GRCm39)	I203L	probably benign	Het
H2-Q10	T	C	17: 35,784,457 (GRCm39)	Y324H	not run	Het
Hcn4	G	A	9: 58,751,582 (GRCm39)	E403K	unknown	Het
Hddc2	T	A	10: 31,189,412 (GRCm39)	M1K	probably null	Het
Hps3	A	G	3: 20,089,329 (GRCm39)	F34S	probably damaging	Het
Ift56	T	A	6: 38,381,422 (GRCm39)	Y319*	probably null	Het
Igdcc4	G	T	9: 65,031,276 (GRCm39)	V405L	possibly damaging	Het
Itpr2	C	A	6: 146,231,006 (GRCm39)	V1215L	probably damaging	Het
Kif26b	T	C	1: 178,742,339 (GRCm39)	S812P	probably damaging	Het
Lgi1	T	A	19: 38,289,713 (GRCm39)	C260S	probably damaging	Het
Lhfpl6	A	G	3: 53,168,020 (GRCm39)	Y198C	probably damaging	Het
Lrp5	T	C	19: 3,699,439 (GRCm39)	D282G	probably benign	Het
Lrpprc	T	C	17: 85,079,567 (GRCm39)	T230A	probably damaging	Het
Lrtm2	A	G	6: 119,297,784 (GRCm39)	W86R	probably benign	Het
Maco1	A	T	4: 134,555,590 (GRCm39)	N294K	possibly damaging	Het
Magi2	G	A	5: 20,563,954 (GRCm39)	G199D	probably damaging	Het
Map1b	C	T	13: 99,644,648 (GRCm39)	R85Q	probably damaging	Het
Marchf7	T	A	2: 60,077,858 (GRCm39)		probably null	Het
Morc1	A	G	16: 48,251,708 (GRCm39)	D2G	probably damaging	Het
Mpp7	A	T	18: 7,351,079 (GRCm39)	F539L	probably damaging	Het
Muc13	A	T	16: 33,634,951 (GRCm39)	I502F	probably damaging	Het
Myh13	A	G	11: 67,255,286 (GRCm39)		probably null	Het
Nat10	C	G	2: 103,578,390 (GRCm39)	L238F	probably damaging	Het
Nckap1	G	A	2: 80,354,885 (GRCm39)	T679I	probably damaging	Het
Npy6r	T	A	18: 44,409,260 (GRCm39)	I227N	probably damaging	Het
Nudt18	A	T	14: 70,815,389 (GRCm39)	M1L	unknown	Het
Or13d1	T	A	4: 52,971,207 (GRCm39)	N195K	probably damaging	Het
Or14a257	G	A	7: 86,138,417 (GRCm39)	T114I	probably damaging	Het
Or4f14	T	A	2: 111,742,637 (GRCm39)	I213L	probably benign	Het
Or8d1	C	A	9: 38,766,412 (GRCm39)	T18K	probably damaging	Het
Or8u8	T	C	2: 86,011,678 (GRCm39)	Y259C	probably damaging	Het
Pcdha3	T	A	18: 37,079,266 (GRCm39)	F3I	probably benign	Het
Pcdhga3	T	A	18: 37,808,917 (GRCm39)	Y457N	possibly damaging	Het
Pclo	A	T	5: 14,719,631 (GRCm39)	Q1256L	unknown	Het
Piezo2	C	T	18: 63,157,543 (GRCm39)	R2389H	probably damaging	Het
Pml	G	T	9: 58,154,496 (GRCm39)	Q126K	probably benign	Het
Ppef2	A	G	5: 92,376,563 (GRCm39)	Y655H	probably damaging	Het
Ppp4r1	T	C	17: 66,147,936 (GRCm39)	V926A	probably damaging	Het
Psg29	A	G	7: 16,945,648 (GRCm39)	D406G	possibly damaging	Het
Ptprf	T	C	4: 118,092,864 (GRCm39)	D517G	probably benign	Het
Ptprg	G	A	14: 12,142,461 (GRCm38)	E371K	probably benign	Het
Pttg1ip2	T	C	5: 5,505,952 (GRCm39)	I110V	probably benign	Het
Rasgrp1	T	C	2: 117,118,424 (GRCm39)	I522V	probably damaging	Het
Rasgrp1	T	A	2: 117,122,178 (GRCm39)	D404V	possibly damaging	Het
Rb1cc1	T	A	1: 6,315,727 (GRCm39)	F541I	probably damaging	Het
Rgsl1	C	A	1: 153,719,847 (GRCm39)		probably null	Het
Rhobtb2	C	T	14: 70,033,397 (GRCm39)	W524*	probably null	Het
Rhox4d	G	A	X: 36,700,645 (GRCm39)	G191E	unknown	Het
Rims1	A	G	1: 22,474,699 (GRCm39)	S211P		Het
Ripor2	A	T	13: 24,854,054 (GRCm39)	Q54L	possibly damaging	Het
Rnf213	A	G	11: 119,372,117 (GRCm39)	I4903V		Het
Robo3	T	A	9: 37,336,111 (GRCm39)	I452F	possibly damaging	Het
Rpl27rt	A	G	18: 34,870,580 (GRCm39)	K38R	probably damaging	Het
Seh1l	C	T	18: 67,916,988 (GRCm39)	H56Y	probably damaging	Het
Sema3b	T	A	9: 107,480,162 (GRCm39)	D192V	probably damaging	Het
Sidt1	A	T	16: 44,106,763 (GRCm39)	C222*	probably null	Het
Skor1	A	G	9: 63,053,385 (GRCm39)	F195L	probably damaging	Het
Slc44a2	A	C	9: 21,259,642 (GRCm39)	K596N	possibly damaging	Het
Smgc	A	G	15: 91,729,696 (GRCm39)	K217E	probably benign	Het
Socs7	C	A	11: 97,267,917 (GRCm39)	H349Q	possibly damaging	Het
Speer4f2	A	G	5: 17,581,540 (GRCm39)	T161A		Het
Spg11	T	C	2: 121,924,026 (GRCm39)		probably null	Het
Ssb	A	G	2: 69,693,624 (GRCm39)	T11A	probably benign	Het
Sun1	A	G	5: 139,232,589 (GRCm39)	S837G	probably damaging	Het
Szt2	A	C	4: 118,220,668 (GRCm39)	S3385A	unknown	Het
Tapbp	T	C	17: 34,139,391 (GRCm39)	V129A	possibly damaging	Het
Thsd1	T	C	8: 22,733,349 (GRCm39)	I132T	possibly damaging	Het
Tm6sf2	T	A	8: 70,530,589 (GRCm39)	V223E	possibly damaging	Het
Tm9sf4	T	A	2: 153,036,267 (GRCm39)	M343K	probably benign	Het
Trank1	C	A	9: 111,195,417 (GRCm39)	P1147Q	probably benign	Het
Trip4	G	A	9: 65,773,757 (GRCm39)	T275M	probably damaging	Het
Tsen34	T	C	7: 3,698,834 (GRCm39)		probably null	Het
Ttn	T	C	2: 76,680,422 (GRCm39)	E1086G	unknown	Het
Ubr4	A	T	4: 139,189,778 (GRCm39)	M853L	unknown	Het
Ubxn11	A	T	4: 133,852,466 (GRCm39)	R352W	probably damaging	Het
Vmn1r35	G	A	6: 66,656,219 (GRCm39)		probably benign	Het
Vmn2r107	C	T	17: 20,595,994 (GRCm39)	T849I	probably benign	Het
Vmn2r93	C	A	17: 18,546,248 (GRCm39)	L707I	probably benign	Het
Wwc1	G	A	11: 35,766,533 (GRCm39)	T574I	probably benign	Het
Zfp143	A	G	7: 109,669,705 (GRCm39)	M45V	probably benign	Het
Zfp518a	T	A	19: 40,902,601 (GRCm39)	N843K	possibly damaging	Het
Zfp87	A	T	13: 67,665,163 (GRCm39)	M433K	probably benign	Het
Zfp873	C	A	10: 81,896,461 (GRCm39)	H397Q	probably damaging	Het
Zscan21	A	T	5: 138,116,110 (GRCm39)		probably benign	Het

Other mutations in Cobl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00338:Cobl	APN	11	12,325,813 (GRCm39)	missense	possibly damaging	0.89
IGL00698:Cobl	APN	11	12,203,722 (GRCm39)	missense	probably benign	0.41
IGL00772:Cobl	APN	11	12,216,985 (GRCm39)	missense	probably benign	0.02
IGL00922:Cobl	APN	11	12,204,866 (GRCm39)	missense	probably damaging	1.00
IGL00985:Cobl	APN	11	12,204,843 (GRCm39)	missense	probably damaging	1.00
IGL01641:Cobl	APN	11	12,259,641 (GRCm39)	nonsense	probably null
IGL01722:Cobl	APN	11	12,203,987 (GRCm39)	missense	probably benign	0.00
IGL01734:Cobl	APN	11	12,204,980 (GRCm39)	splice site	probably benign
IGL01924:Cobl	APN	11	12,204,596 (GRCm39)	missense	probably benign	0.30
IGL02105:Cobl	APN	11	12,199,651 (GRCm39)	missense	probably damaging	1.00
IGL02326:Cobl	APN	11	12,336,712 (GRCm39)	missense	possibly damaging	0.69
IGL02342:Cobl	APN	11	12,203,672 (GRCm39)	missense	possibly damaging	0.64
IGL02426:Cobl	APN	11	12,204,351 (GRCm39)	nonsense	probably null
IGL02754:Cobl	APN	11	12,204,370 (GRCm39)	missense	probably damaging	1.00
IGL02754:Cobl	APN	11	12,204,371 (GRCm39)	missense	probably damaging	1.00
IGL02811:Cobl	APN	11	12,203,285 (GRCm39)	missense	possibly damaging	0.56
IGL02859:Cobl	APN	11	12,319,602 (GRCm39)	missense	probably damaging	1.00
IGL02999:Cobl	APN	11	12,293,869 (GRCm39)	missense	possibly damaging	0.71
IGL03030:Cobl	APN	11	12,204,241 (GRCm39)	missense	possibly damaging	0.80
IGL03191:Cobl	APN	11	12,203,364 (GRCm39)	missense	probably benign	0.00
PIT4418001:Cobl	UTSW	11	12,206,240 (GRCm39)	missense	possibly damaging	0.79
PIT4480001:Cobl	UTSW	11	12,203,592 (GRCm39)	missense	probably benign
PIT4495001:Cobl	UTSW	11	12,204,596 (GRCm39)	missense	probably benign	0.00
R0031:Cobl	UTSW	11	12,204,945 (GRCm39)	missense	probably benign	0.36
R0241:Cobl	UTSW	11	12,204,524 (GRCm39)	missense	probably benign	0.25
R0241:Cobl	UTSW	11	12,204,524 (GRCm39)	missense	probably benign	0.25
R0322:Cobl	UTSW	11	12,217,072 (GRCm39)	missense	probably damaging	1.00
R0597:Cobl	UTSW	11	12,204,699 (GRCm39)	missense	probably benign	0.24
R0733:Cobl	UTSW	11	12,315,167 (GRCm39)	missense	probably benign	0.31
R0734:Cobl	UTSW	11	12,325,971 (GRCm39)	missense	probably damaging	1.00
R0784:Cobl	UTSW	11	12,216,843 (GRCm39)	splice site	probably benign
R0884:Cobl	UTSW	11	12,325,908 (GRCm39)	missense	possibly damaging	0.89
R1065:Cobl	UTSW	11	12,204,327 (GRCm39)	missense	possibly damaging	0.67
R1331:Cobl	UTSW	11	12,325,853 (GRCm39)	missense	probably damaging	0.96
R1892:Cobl	UTSW	11	12,203,258 (GRCm39)	missense	probably damaging	0.99
R2847:Cobl	UTSW	11	12,328,342 (GRCm39)	missense	probably damaging	1.00
R2848:Cobl	UTSW	11	12,328,342 (GRCm39)	missense	probably damaging	1.00
R3407:Cobl	UTSW	11	12,325,830 (GRCm39)	missense	probably damaging	1.00
R4627:Cobl	UTSW	11	12,201,093 (GRCm39)	missense	probably damaging	1.00
R4662:Cobl	UTSW	11	12,203,672 (GRCm39)	missense	probably benign	0.08
R4677:Cobl	UTSW	11	12,336,665 (GRCm39)	missense	possibly damaging	0.93
R4844:Cobl	UTSW	11	12,204,740 (GRCm39)	missense	probably benign	0.10
R4942:Cobl	UTSW	11	12,204,185 (GRCm39)	missense	probably damaging	0.99
R5158:Cobl	UTSW	11	12,206,198 (GRCm39)	missense	possibly damaging	0.84
R5195:Cobl	UTSW	11	12,203,565 (GRCm39)	missense	probably benign	0.02
R5255:Cobl	UTSW	11	12,325,825 (GRCm39)	missense	probably damaging	1.00
R5588:Cobl	UTSW	11	12,293,886 (GRCm39)	nonsense	probably null
R5637:Cobl	UTSW	11	12,246,531 (GRCm39)	intron	probably benign
R5643:Cobl	UTSW	11	12,256,948 (GRCm39)	splice site	probably benign
R5749:Cobl	UTSW	11	12,216,965 (GRCm39)	missense	possibly damaging	0.86
R5953:Cobl	UTSW	11	12,206,220 (GRCm39)	missense	probably benign	0.00
R6000:Cobl	UTSW	11	12,319,684 (GRCm39)	missense	probably benign	0.08
R6373:Cobl	UTSW	11	12,203,118 (GRCm39)	missense	probably damaging	1.00
R7034:Cobl	UTSW	11	12,204,177 (GRCm39)	missense	probably damaging	1.00
R7071:Cobl	UTSW	11	12,204,795 (GRCm39)	missense	probably benign	0.00
R7077:Cobl	UTSW	11	12,203,441 (GRCm39)	missense	probably benign	0.04
R7078:Cobl	UTSW	11	12,328,271 (GRCm39)	missense	probably damaging	1.00
R7099:Cobl	UTSW	11	12,246,540 (GRCm39)	missense
R7153:Cobl	UTSW	11	12,204,128 (GRCm39)	missense	probably damaging	1.00
R7519:Cobl	UTSW	11	12,203,124 (GRCm39)	missense	probably damaging	1.00
R7767:Cobl	UTSW	11	12,362,117 (GRCm39)	start gained	probably benign
R7772:Cobl	UTSW	11	12,204,488 (GRCm39)	missense	probably benign	0.29
R7841:Cobl	UTSW	11	12,203,324 (GRCm39)	missense	probably damaging	1.00
R7845:Cobl	UTSW	11	12,315,139 (GRCm39)	missense	probably benign	0.35
R8026:Cobl	UTSW	11	12,203,459 (GRCm39)	missense	probably benign	0.01
R8118:Cobl	UTSW	11	12,204,834 (GRCm39)	missense	probably benign	0.03
R8192:Cobl	UTSW	11	12,199,745 (GRCm39)	missense	probably benign	0.07
R8320:Cobl	UTSW	11	12,217,001 (GRCm39)	missense	probably damaging	1.00
R8338:Cobl	UTSW	11	12,203,696 (GRCm39)	missense	probably benign	0.41
R9319:Cobl	UTSW	11	12,203,648 (GRCm39)	missense	probably benign	0.00
R9497:Cobl	UTSW	11	12,203,501 (GRCm39)	missense	probably benign	0.00
R9501:Cobl	UTSW	11	12,328,235 (GRCm39)	missense	possibly damaging	0.94
Z1176:Cobl	UTSW	11	12,325,827 (GRCm39)	missense	probably damaging	1.00
Z1176:Cobl	UTSW	11	12,319,645 (GRCm39)	missense	probably damaging	1.00
Z1176:Cobl	UTSW	11	12,203,433 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- AGTTGCAAACCCAAGACTTCTTTC -3'
(R):5'- CTGCATGGACTTCAGCTGTTG -3'

Sequencing Primer
(F):5'- GCAAACCCAAGACTTCTTTCTTATAG -3'
(R):5'- CTGCATGGACTTCAGCTGTTGATAAC -3'

Posted On

2019-10-07