Mutagenetix > Incidental Mutation

Incidental Mutation 'R7449:Trrap'

577550

Institutional Source

Beutler Lab

Gene Symbol

Trrap

Ensembl Gene

ENSMUSG00000045482

Gene Name

transformation/transcription domain-associated protein

Synonyms

transactivation/transformation-domain associated protein

MMRRC Submission

045524-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7449 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

144767732-144859778 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 144851209 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 3516 (Y3516C)

Ref Sequence

ENSEMBL: ENSMUSP00000098035 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000038980] [ENSMUST00000094120] [ENSMUST00000100467] [ENSMUST00000213013]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000038980
AA Change: Y3498C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000042544
Gene: ENSMUSG00000045482
AA Change: Y3498C

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1664	2e-6	SMART
low complexity region	1832	1843	N/A	INTRINSIC
low complexity region	1866	1881	N/A	INTRINSIC
low complexity region	2289	2303	N/A	INTRINSIC
Pfam:FAT	2830	3174	4.7e-69	PFAM
low complexity region	3363	3376	N/A	INTRINSIC
low complexity region	3407	3418	N/A	INTRINSIC
PI3Kc	3509	3798	5.11e-8	SMART
FATC	3797	3829	1.89e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000094120
AA Change: Y3527C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000091668
Gene: ENSMUSG00000045482
AA Change: Y3527C

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1682	2e-6	SMART
low complexity region	1850	1861	N/A	INTRINSIC
low complexity region	1884	1899	N/A	INTRINSIC
low complexity region	2307	2321	N/A	INTRINSIC
Pfam:FAT	2848	3203	1.1e-68	PFAM
low complexity region	3392	3405	N/A	INTRINSIC
low complexity region	3436	3447	N/A	INTRINSIC
PI3Kc	3538	3827	5.11e-8	SMART
FATC	3826	3858	1.89e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000100467
AA Change: Y3516C

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000098035
Gene: ENSMUSG00000045482
AA Change: Y3516C

Domain	Start	End	E-Value	Type
low complexity region	482	527	N/A	INTRINSIC
low complexity region	529	540	N/A	INTRINSIC
Blast:PI3Kc	765	864	1e-13	BLAST
SCOP:d1gw5a_	1184	1664	2e-6	SMART
low complexity region	1832	1843	N/A	INTRINSIC
low complexity region	1866	1881	N/A	INTRINSIC
low complexity region	2289	2303	N/A	INTRINSIC
Pfam:FAT	2830	3174	4.7e-69	PFAM
low complexity region	3381	3394	N/A	INTRINSIC
low complexity region	3425	3436	N/A	INTRINSIC
PI3Kc	3527	3816	5.11e-8	SMART
FATC	3815	3847	1.89e-3	SMART

Predicted Effect

SMART Domains

Protein: ENSMUSP00000122021
Gene: ENSMUSG00000045482
AA Change: Y3255C

Domain	Start	End	E-Value	Type
low complexity region	197	242	N/A	INTRINSIC
low complexity region	244	255	N/A	INTRINSIC
SCOP:d1gw5a_	474	1003	9e-7	SMART
Blast:PI3Kc	480	579	1e-13	BLAST
low complexity region	1083	1092	N/A	INTRINSIC
low complexity region	1572	1583	N/A	INTRINSIC
low complexity region	1606	1621	N/A	INTRINSIC
low complexity region	2029	2043	N/A	INTRINSIC
Pfam:FAT	2570	2914	1.5e-69	PFAM
low complexity region	3121	3134	N/A	INTRINSIC
low complexity region	3165	3176	N/A	INTRINSIC
PI3Kc	3267	3556	5.11e-8	SMART
FATC	3555	3587	1.89e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000213013
AA Change: Y3528C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a large multidomain protein of the phosphoinositide 3-kinase-related kinases (PIKK) family. The encoded protein is a common component of many histone acetyltransferase (HAT) complexes and plays a role in transcription and DNA repair by recruiting HAT complexes to chromatin. Deregulation of this gene may play a role in several types of cancer including glioblastoma multiforme. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
PHENOTYPE: Homozygous embryos die prior to E3.5 and exhibit embryonic and extraembryonic tissue disorganization. Mitotic abnormalities were also noted in homozygous cells. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca16	T	A	7: 120,435,908 (GRCm38)	Y306N	possibly damaging	Het
Adgrv1	A	T	13: 81,499,073 (GRCm38)	V3116D	probably damaging	Het
Adssl1	A	T	12: 112,634,151 (GRCm38)	T185S	probably damaging	Het
Ago2	G	A	15: 73,146,499 (GRCm38)	P30L	probably damaging	Het
Atp5j	C	T	16: 84,831,363 (GRCm38)	V44M	probably benign	Het
Atp7b	T	A	8: 22,011,849 (GRCm38)	I833F	probably damaging	Het
Birc6	T	A	17: 74,702,341 (GRCm38)	N4869K	probably benign	Het
Cacna1c	C	T	6: 118,602,349 (GRCm38)	D1796N		Het
Ccng2	C	G	5: 93,273,343 (GRCm38)	S237R	probably benign	Het
Cnga1	A	G	5: 72,605,304 (GRCm38)	I289T	probably benign	Het
Crybg1	C	A	10: 44,004,519 (GRCm38)	E224D	probably benign	Het
Dysf	T	C	6: 84,137,380 (GRCm38)	L1217P	possibly damaging	Het
Ebf2	T	A	14: 67,410,020 (GRCm38)	N339K	probably damaging	Het
Eva1c	T	C	16: 90,876,193 (GRCm38)		probably null	Het
Fam172a	A	G	13: 77,759,442 (GRCm38)	I41V	probably damaging	Het
Fam184a	T	C	10: 53,698,634 (GRCm38)	E293G	probably damaging	Het
Folh1	G	A	7: 86,731,748 (GRCm38)	P506S	probably benign	Het
Ftcd	A	T	10: 76,580,163 (GRCm38)	K210N	probably benign	Het
Gdf6	T	A	4: 9,844,494 (GRCm38)	V6D	possibly damaging	Het
Ghitm	C	A	14: 37,131,581 (GRCm38)	G101C	probably damaging	Het
Gimap5	T	A	6: 48,752,904 (GRCm38)	V136D	probably damaging	Het
Gm9745	T	A	13: 8,943,304 (GRCm38)	H51L	probably damaging	Het
Grm4	C	T	17: 27,435,371 (GRCm38)	G535D	probably damaging	Het
Gse1	T	C	8: 120,229,711 (GRCm38)	S314P	unknown	Het
Hnrnpdl	A	G	5: 100,037,155 (GRCm38)	I279T	probably damaging	Het
Itpr1	T	C	6: 108,389,384 (GRCm38)	S923P	probably damaging	Het
Krt39	C	A	11: 99,518,061 (GRCm38)	C303F	probably benign	Het
Lrrn1	T	C	6: 107,568,521 (GRCm38)	S427P	possibly damaging	Het
Lrrn3	T	A	12: 41,453,488 (GRCm38)	R277W	probably damaging	Het
Ltb4r1	T	C	14: 55,767,918 (GRCm38)	L226P	probably damaging	Het
Map1b	C	T	13: 99,508,140 (GRCm38)	R85Q	probably damaging	Het
Mcoln1	T	C	8: 3,507,285 (GRCm38)	L125P	probably damaging	Het
Nid1	T	G	13: 13,482,051 (GRCm38)	V589G	probably damaging	Het
Nlrp5	T	G	7: 23,417,526 (GRCm38)	F225C	probably benign	Het
Notch3	C	T	17: 32,157,966 (GRCm38)	A322T	probably damaging	Het
Olfr299	A	G	7: 86,465,855 (GRCm38)	H148R	probably benign	Het
Olfr69	G	T	7: 103,767,819 (GRCm38)	Q193K	probably benign	Het
Olfr854	T	A	9: 19,566,866 (GRCm38)	T173S	probably benign	Het
Otogl	A	G	10: 107,803,663 (GRCm38)	C1363R	probably damaging	Het
Ovol1	T	A	19: 5,553,597 (GRCm38)	D92V	probably benign	Het
Pigt	T	C	2: 164,502,499 (GRCm38)	L356P	probably damaging	Het
Plekhg3	A	G	12: 76,566,222 (GRCm38)	Q434R	probably damaging	Het
Plekhh1	T	C	12: 79,079,552 (GRCm38)	F1344L	probably benign	Het
Psmd3	T	A	11: 98,695,551 (GRCm38)	L515Q	probably damaging	Het
Pus1	A	G	5: 110,774,586 (GRCm38)	L405P	probably damaging	Het
Qtrt2	T	A	16: 43,881,032 (GRCm38)	H55L	probably benign	Het
Rasgrp1	T	C	2: 117,287,943 (GRCm38)	I522V	probably damaging	Het
Raver2	T	A	4: 101,102,663 (GRCm38)	H113Q	probably damaging	Het
Recql4	T	C	15: 76,705,565 (GRCm38)	D760G	unknown	Het
Rhobtb3	C	T	13: 75,910,741 (GRCm38)	V313M	probably benign	Het
Rhox4d	G	A	X: 37,518,992 (GRCm38)	G191E	unknown	Het
Rictor	C	T	15: 6,772,154 (GRCm38)	S441L	probably benign	Het
Rnf185	T	C	11: 3,426,578 (GRCm38)	Q135R	probably benign	Het
Sema3f	T	C	9: 107,684,036 (GRCm38)	S584G	probably damaging	Het
Sh3pxd2a	T	A	19: 47,267,652 (GRCm38)	T904S	probably benign	Het
Slc4a10	T	C	2: 62,303,946 (GRCm38)	V1002A	probably benign	Het
Taf1b	T	C	12: 24,504,993 (GRCm38)	I55T	probably benign	Het
Tchh	CTCCGCCGGGAGCAAGAGCTCCGCCGGGAGCAAGAGTTCCGCCGGGAGCAAGAGCTCCGCCGGGAGCAAGAGTTCCGCCGGGAGCAAGAGCTCCGCC	CTCCGCCGGGAGCAAGAGCTCCGCCGGGAGCAAGAGTTCCGCCGGGAGCAAGAGCTCCGCC	3: 93,446,708 (GRCm38)		probably benign	Het
Tenm4	A	T	7: 96,874,213 (GRCm38)	D1654V	possibly damaging	Het
Tgfb1	G	A	7: 25,704,838 (GRCm38)	V357M	probably damaging	Het
Tnxb	C	T	17: 34,703,361 (GRCm38)	P2383S	possibly damaging	Het
Trpm1	A	T	7: 64,208,975 (GRCm38)	M382L	probably benign	Het
Txnrd1	T	A	10: 82,885,233 (GRCm38)	Y494*	probably null	Het
Ubr2	C	T	17: 46,964,788 (GRCm38)	E811K	probably damaging	Het
Ubxn4	T	A	1: 128,244,543 (GRCm38)	F25I	possibly damaging	Het
Vmn2r117	A	G	17: 23,459,895 (GRCm38)	M785T	probably damaging	Het
Vmn2r99	G	A	17: 19,379,145 (GRCm38)	D364N	probably benign	Het
Vps45	C	A	3: 96,047,136 (GRCm38)		probably null	Het
Wdr25	A	C	12: 109,026,441 (GRCm38)	H426P	probably damaging	Het
Wdr83	A	T	8: 85,079,681 (GRCm38)	W136R	probably damaging	Het
Xylt1	A	C	7: 117,592,005 (GRCm38)	I343L	possibly damaging	Het
Znrd1as	A	G	17: 36,964,383 (GRCm38)	D16G	probably damaging	Het
Zscan4b	T	C	7: 10,904,058 (GRCm38)	Q53R	possibly damaging	Het

Other mutations in Trrap

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Trrap	APN	5	144,779,974 (GRCm38)	splice site	probably benign
IGL00470:Trrap	APN	5	144,818,038 (GRCm38)	missense	probably damaging	1.00
IGL00490:Trrap	APN	5	144,825,225 (GRCm38)	missense	probably benign	0.40
IGL01072:Trrap	APN	5	144,784,255 (GRCm38)	splice site	probably benign
IGL01087:Trrap	APN	5	144,846,539 (GRCm38)	missense	probably damaging	0.99
IGL01300:Trrap	APN	5	144,804,818 (GRCm38)	missense	probably damaging	1.00
IGL01350:Trrap	APN	5	144,830,969 (GRCm38)	missense	possibly damaging	0.92
IGL01410:Trrap	APN	5	144,831,021 (GRCm38)	missense	probably benign	0.00
IGL01571:Trrap	APN	5	144,833,287 (GRCm38)	splice site	probably benign
IGL01748:Trrap	APN	5	144,833,340 (GRCm38)	missense	probably damaging	1.00
IGL01839:Trrap	APN	5	144,821,875 (GRCm38)	missense	probably damaging	1.00
IGL01976:Trrap	APN	5	144,856,989 (GRCm38)	missense	probably benign	0.00
IGL02075:Trrap	APN	5	144,828,494 (GRCm38)	missense	probably benign	0.00
IGL02127:Trrap	APN	5	144,816,433 (GRCm38)	missense	probably benign	0.22
IGL02131:Trrap	APN	5	144,840,436 (GRCm38)	missense	probably damaging	1.00
IGL02287:Trrap	APN	5	144,832,538 (GRCm38)	missense	probably damaging	1.00
IGL02301:Trrap	APN	5	144,777,917 (GRCm38)	missense	probably benign	0.05
IGL02336:Trrap	APN	5	144,798,390 (GRCm38)	missense	probably benign	0.39
IGL02526:Trrap	APN	5	144,824,550 (GRCm38)	missense	probably benign	0.00
IGL02873:Trrap	APN	5	144,841,079 (GRCm38)	splice site	probably benign
IGL02953:Trrap	APN	5	144,815,964 (GRCm38)	missense	probably damaging	0.99
IGL03404:Trrap	APN	5	144,833,186 (GRCm38)	missense	probably benign	0.00
Buffer	UTSW	5	144,834,204 (GRCm38)	missense	probably benign	0.06
Card-tower	UTSW	5	144,804,766 (GRCm38)	missense	probably damaging	1.00
Cookie	UTSW	5	144,794,049 (GRCm38)	missense	probably damaging	1.00
Glass_house	UTSW	5	144,845,477 (GRCm38)	missense	possibly damaging	0.67
Immovable	UTSW	5	144,790,855 (GRCm38)	missense	possibly damaging	0.66
R5049_trrap_520	UTSW	5	144,826,717 (GRCm38)	missense	probably damaging	1.00
R7167_Trrap_977	UTSW	5	144,839,614 (GRCm38)	missense	probably benign	0.39
vitreous	UTSW	5	144,805,727 (GRCm38)	missense	probably damaging	1.00
PIT4243001:Trrap	UTSW	5	144,796,971 (GRCm38)	missense	probably benign	0.00
PIT4466001:Trrap	UTSW	5	144,828,600 (GRCm38)	missense	probably benign	0.02
R0062:Trrap	UTSW	5	144,782,193 (GRCm38)	splice site	probably benign
R0062:Trrap	UTSW	5	144,782,193 (GRCm38)	splice site	probably benign
R0112:Trrap	UTSW	5	144,822,761 (GRCm38)	nonsense	probably null
R0126:Trrap	UTSW	5	144,805,750 (GRCm38)	nonsense	probably null
R0257:Trrap	UTSW	5	144,804,235 (GRCm38)	missense	probably benign	0.31
R0325:Trrap	UTSW	5	144,816,395 (GRCm38)	missense	probably benign	0.05
R0376:Trrap	UTSW	5	144,816,339 (GRCm38)	missense	probably benign	0.03
R0396:Trrap	UTSW	5	144,814,556 (GRCm38)	missense	probably damaging	0.99
R0448:Trrap	UTSW	5	144,839,567 (GRCm38)	missense	possibly damaging	0.66
R0454:Trrap	UTSW	5	144,846,477 (GRCm38)	missense	probably damaging	1.00
R0711:Trrap	UTSW	5	144,853,499 (GRCm38)	missense	probably damaging	1.00
R0827:Trrap	UTSW	5	144,814,830 (GRCm38)	missense	probably benign	0.00
R1005:Trrap	UTSW	5	144,805,727 (GRCm38)	missense	probably damaging	1.00
R1147:Trrap	UTSW	5	144,804,766 (GRCm38)	missense	probably damaging	1.00
R1147:Trrap	UTSW	5	144,804,766 (GRCm38)	missense	probably damaging	1.00
R1179:Trrap	UTSW	5	144,777,939 (GRCm38)	missense	possibly damaging	0.94
R1218:Trrap	UTSW	5	144,816,409 (GRCm38)	missense	probably damaging	1.00
R1264:Trrap	UTSW	5	144,789,599 (GRCm38)	splice site	probably benign
R1374:Trrap	UTSW	5	144,846,618 (GRCm38)	missense	probably damaging	1.00
R1401:Trrap	UTSW	5	144,857,422 (GRCm38)	missense	possibly damaging	0.93
R1480:Trrap	UTSW	5	144,818,313 (GRCm38)	missense	probably benign
R1538:Trrap	UTSW	5	144,837,202 (GRCm38)	missense	possibly damaging	0.65
R1751:Trrap	UTSW	5	144,814,575 (GRCm38)	critical splice donor site	probably null
R1779:Trrap	UTSW	5	144,828,590 (GRCm38)	missense	probably benign	0.01
R1782:Trrap	UTSW	5	144,822,703 (GRCm38)	missense	possibly damaging	0.93
R1792:Trrap	UTSW	5	144,853,586 (GRCm38)	missense	possibly damaging	0.87
R1859:Trrap	UTSW	5	144,830,951 (GRCm38)	missense	probably benign	0.04
R1861:Trrap	UTSW	5	144,815,917 (GRCm38)	splice site	probably null
R1902:Trrap	UTSW	5	144,816,053 (GRCm38)	missense	probably damaging	1.00
R1903:Trrap	UTSW	5	144,816,053 (GRCm38)	missense	probably damaging	1.00
R2021:Trrap	UTSW	5	144,853,488 (GRCm38)	missense	possibly damaging	0.94
R2026:Trrap	UTSW	5	144,803,044 (GRCm38)	missense	possibly damaging	0.86
R2036:Trrap	UTSW	5	144,828,562 (GRCm38)	missense	probably benign	0.08
R2099:Trrap	UTSW	5	144,782,239 (GRCm38)	missense	possibly damaging	0.46
R2108:Trrap	UTSW	5	144,825,874 (GRCm38)	missense	probably benign	0.01
R2113:Trrap	UTSW	5	144,844,211 (GRCm38)	missense	probably damaging	1.00
R2174:Trrap	UTSW	5	144,821,855 (GRCm38)	missense	probably benign	0.40
R2442:Trrap	UTSW	5	144,817,966 (GRCm38)	missense	probably damaging	1.00
R2568:Trrap	UTSW	5	144,843,369 (GRCm38)	critical splice donor site	probably null
R3442:Trrap	UTSW	5	144,792,252 (GRCm38)	missense	probably benign	0.03
R3853:Trrap	UTSW	5	144,792,165 (GRCm38)	missense	probably damaging	1.00
R4401:Trrap	UTSW	5	144,843,318 (GRCm38)	missense	possibly damaging	0.60
R4493:Trrap	UTSW	5	144,831,048 (GRCm38)	missense	probably benign	0.21
R4524:Trrap	UTSW	5	144,825,321 (GRCm38)	missense	probably benign	0.38
R4569:Trrap	UTSW	5	144,792,118 (GRCm38)	missense	probably benign	0.13
R4672:Trrap	UTSW	5	144,785,480 (GRCm38)	missense	probably damaging	0.97
R4732:Trrap	UTSW	5	144,816,570 (GRCm38)	missense	probably damaging	1.00
R4733:Trrap	UTSW	5	144,816,570 (GRCm38)	missense	probably damaging	1.00
R4791:Trrap	UTSW	5	144,803,277 (GRCm38)	missense	probably damaging	1.00
R4795:Trrap	UTSW	5	144,832,488 (GRCm38)	missense	probably benign	0.06
R4827:Trrap	UTSW	5	144,800,948 (GRCm38)	missense	probably benign	0.02
R4839:Trrap	UTSW	5	144,845,592 (GRCm38)	missense	probably damaging	1.00
R4915:Trrap	UTSW	5	144,805,735 (GRCm38)	missense	probably damaging	0.99
R4951:Trrap	UTSW	5	144,805,720 (GRCm38)	missense	possibly damaging	0.65
R4959:Trrap	UTSW	5	144,856,960 (GRCm38)	missense	probably damaging	1.00
R5049:Trrap	UTSW	5	144,826,717 (GRCm38)	missense	probably damaging	1.00
R5074:Trrap	UTSW	5	144,851,179 (GRCm38)	missense	probably damaging	1.00
R5236:Trrap	UTSW	5	144,817,786 (GRCm38)	missense	probably benign	0.07
R5281:Trrap	UTSW	5	144,813,503 (GRCm38)	missense	probably benign	0.13
R5322:Trrap	UTSW	5	144,844,224 (GRCm38)	missense	probably damaging	1.00
R5457:Trrap	UTSW	5	144,849,977 (GRCm38)	missense	probably damaging	1.00
R5590:Trrap	UTSW	5	144,782,265 (GRCm38)	missense	probably benign	0.05
R5799:Trrap	UTSW	5	144,830,945 (GRCm38)	missense	probably benign
R5885:Trrap	UTSW	5	144,794,793 (GRCm38)	missense	probably damaging	1.00
R5905:Trrap	UTSW	5	144,849,920 (GRCm38)	missense	possibly damaging	0.95
R5908:Trrap	UTSW	5	144,786,708 (GRCm38)	missense	probably damaging	0.96
R5956:Trrap	UTSW	5	144,807,391 (GRCm38)	splice site	silent
R5992:Trrap	UTSW	5	144,810,184 (GRCm38)	missense	probably benign	0.00
R6017:Trrap	UTSW	5	144,844,241 (GRCm38)	missense	probably damaging	1.00
R6029:Trrap	UTSW	5	144,825,914 (GRCm38)	missense	possibly damaging	0.75
R6029:Trrap	UTSW	5	144,817,679 (GRCm38)	missense	possibly damaging	0.94
R6117:Trrap	UTSW	5	144,802,961 (GRCm38)	missense	possibly damaging	0.78
R6166:Trrap	UTSW	5	144,781,981 (GRCm38)	missense	possibly damaging	0.66
R6234:Trrap	UTSW	5	144,839,713 (GRCm38)	splice site	probably null
R6288:Trrap	UTSW	5	144,811,992 (GRCm38)	missense	probably damaging	1.00
R6290:Trrap	UTSW	5	144,805,018 (GRCm38)	missense	probably damaging	1.00
R6316:Trrap	UTSW	5	144,813,526 (GRCm38)	missense	probably benign	0.02
R6398:Trrap	UTSW	5	144,790,870 (GRCm38)	missense	possibly damaging	0.83
R6413:Trrap	UTSW	5	144,784,046 (GRCm38)	missense	possibly damaging	0.83
R6499:Trrap	UTSW	5	144,857,002 (GRCm38)	missense	probably damaging	1.00
R6529:Trrap	UTSW	5	144,834,204 (GRCm38)	missense	probably benign	0.06
R6574:Trrap	UTSW	5	144,815,550 (GRCm38)	critical splice donor site	probably null
R6631:Trrap	UTSW	5	144,771,650 (GRCm38)	missense	possibly damaging	0.94
R6727:Trrap	UTSW	5	144,856,950 (GRCm38)	missense	probably damaging	1.00
R6776:Trrap	UTSW	5	144,851,256 (GRCm38)	nonsense	probably null
R6914:Trrap	UTSW	5	144,784,043 (GRCm38)	missense	possibly damaging	0.83
R6942:Trrap	UTSW	5	144,784,043 (GRCm38)	missense	possibly damaging	0.83
R6945:Trrap	UTSW	5	144,790,855 (GRCm38)	missense	possibly damaging	0.66
R7023:Trrap	UTSW	5	144,792,154 (GRCm38)	missense	possibly damaging	0.64
R7107:Trrap	UTSW	5	144,797,135 (GRCm38)	missense	probably benign	0.05
R7139:Trrap	UTSW	5	144,803,178 (GRCm38)	missense	possibly damaging	0.65
R7148:Trrap	UTSW	5	144,821,803 (GRCm38)	missense	possibly damaging	0.77
R7167:Trrap	UTSW	5	144,839,614 (GRCm38)	missense	probably benign	0.39
R7171:Trrap	UTSW	5	144,794,049 (GRCm38)	missense	probably damaging	1.00
R7205:Trrap	UTSW	5	144,842,707 (GRCm38)	missense	possibly damaging	0.94
R7215:Trrap	UTSW	5	144,797,135 (GRCm38)	missense	probably benign	0.05
R7255:Trrap	UTSW	5	144,858,954 (GRCm38)	missense	probably damaging	1.00
R7261:Trrap	UTSW	5	144,845,477 (GRCm38)	missense	possibly damaging	0.67
R7264:Trrap	UTSW	5	144,814,523 (GRCm38)	missense	probably benign	0.05
R7372:Trrap	UTSW	5	144,789,398 (GRCm38)	missense	probably benign
R7447:Trrap	UTSW	5	144,839,474 (GRCm38)	missense	probably damaging	0.97
R7655:Trrap	UTSW	5	144,842,612 (GRCm38)	missense	probably damaging	1.00
R7656:Trrap	UTSW	5	144,842,612 (GRCm38)	missense	probably damaging	1.00
R7662:Trrap	UTSW	5	144,832,511 (GRCm38)	missense	probably benign	0.00
R7716:Trrap	UTSW	5	144,777,146 (GRCm38)	missense	possibly damaging	0.73
R8143:Trrap	UTSW	5	144,835,897 (GRCm38)	splice site	probably null
R8183:Trrap	UTSW	5	144,828,533 (GRCm38)	missense	probably benign	0.01
R8265:Trrap	UTSW	5	144,785,534 (GRCm38)	missense	possibly damaging	0.53
R8273:Trrap	UTSW	5	144,791,165 (GRCm38)	missense	probably damaging	1.00
R8556:Trrap	UTSW	5	144,825,937 (GRCm38)	missense	probably benign	0.44
R8674:Trrap	UTSW	5	144,791,032 (GRCm38)	missense	probably benign	0.02
R8777:Trrap	UTSW	5	144,837,139 (GRCm38)	missense	probably benign	0.10
R8777-TAIL:Trrap	UTSW	5	144,837,139 (GRCm38)	missense	probably benign	0.10
R8817:Trrap	UTSW	5	144,845,538 (GRCm38)	missense	probably damaging	1.00
R8841:Trrap	UTSW	5	144,844,211 (GRCm38)	missense	probably damaging	1.00
R8871:Trrap	UTSW	5	144,821,839 (GRCm38)	missense	probably benign	0.30
R8937:Trrap	UTSW	5	144,820,253 (GRCm38)	missense	probably damaging	1.00
R8966:Trrap	UTSW	5	144,803,352 (GRCm38)	missense	probably damaging	0.96
R9010:Trrap	UTSW	5	144,846,416 (GRCm38)	missense	probably damaging	1.00
R9095:Trrap	UTSW	5	144,797,151 (GRCm38)	missense	probably damaging	1.00
R9127:Trrap	UTSW	5	144,831,020 (GRCm38)	missense	probably benign	0.16
R9132:Trrap	UTSW	5	144,789,552 (GRCm38)	missense	probably benign	0.03
R9224:Trrap	UTSW	5	144,771,239 (GRCm38)	missense	possibly damaging	0.70
R9338:Trrap	UTSW	5	144,791,115 (GRCm38)	missense	probably benign
R9380:Trrap	UTSW	5	144,833,171 (GRCm38)	missense	probably benign
R9404:Trrap	UTSW	5	144,815,415 (GRCm38)	missense	possibly damaging	0.85
R9457:Trrap	UTSW	5	144,826,668 (GRCm38)	missense	probably damaging	1.00
R9464:Trrap	UTSW	5	144,826,707 (GRCm38)	missense	probably damaging	0.99
R9504:Trrap	UTSW	5	144,806,094 (GRCm38)	missense	probably damaging	1.00
R9583:Trrap	UTSW	5	144,840,520 (GRCm38)	missense	probably damaging	1.00
R9584:Trrap	UTSW	5	144,840,520 (GRCm38)	missense	probably damaging	1.00
R9585:Trrap	UTSW	5	144,840,520 (GRCm38)	missense	probably damaging	1.00
R9608:Trrap	UTSW	5	144,843,318 (GRCm38)	missense	possibly damaging	0.60
R9728:Trrap	UTSW	5	144,789,383 (GRCm38)	missense	probably benign	0.22
R9782:Trrap	UTSW	5	144,821,906 (GRCm38)	missense	probably damaging	0.99
X0060:Trrap	UTSW	5	144,843,361 (GRCm38)	missense	probably damaging	0.96
Z1088:Trrap	UTSW	5	144,834,197 (GRCm38)	missense	probably benign	0.00
Z1177:Trrap	UTSW	5	144,819,708 (GRCm38)	missense	probably damaging	1.00
Z1177:Trrap	UTSW	5	144,810,344 (GRCm38)	missense
Z1177:Trrap	UTSW	5	144,856,951 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GTCCATCCTAGGTTACCGTG -3'
(R):5'- ACGGCTGCTGCTCCTTAAAG -3'

Sequencing Primer
(F):5'- TAGGTTACCGTGGCCGCTG -3'
(R):5'- TTAAAGCTTCCCATCAGCGG -3'

Posted On

2019-10-07