Mutagenetix > Incidental Mutation

Incidental Mutation 'R7468:Prdm15'

578990

Institutional Source

Beutler Lab

Gene Symbol

Prdm15

Ensembl Gene

ENSMUSG00000014039

Gene Name

PR domain containing 15

Synonyms

Zfp298, E130018M06Rik

MMRRC Submission

045542-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7468 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

97592667-97653050 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to C at 97636842 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 158 (Y158*)

Ref Sequence

ENSEMBL: ENSMUSP00000113791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095849] [ENSMUST00000119200] [ENSMUST00000121584] [ENSMUST00000135505] [ENSMUST00000142295]

AlphaFold

E9Q8T2

Predicted Effect

probably null
Transcript: ENSMUST00000095849
AA Change: Y184*

SMART Domains

Protein: ENSMUSP00000093533
Gene: ENSMUSG00000014039
AA Change: Y184*

Domain	Start	End	E-Value	Type
SET	75	191	5.96e-1	SMART
ZnF_C2H2	223	245	3.99e0	SMART
low complexity region	290	303	N/A	INTRINSIC
ZnF_C2H2	402	424	3.89e-3	SMART
ZnF_C2H2	434	457	2.75e-3	SMART
ZnF_C2H2	468	488	1.88e2	SMART
ZnF_C2H2	495	517	5.42e-2	SMART
ZnF_C2H2	522	544	1.36e-2	SMART
ZnF_C2H2	571	593	6.23e-2	SMART
ZnF_C2H2	598	620	2.75e-3	SMART
low complexity region	642	657	N/A	INTRINSIC
ZnF_C2H2	661	684	2.17e-1	SMART
ZnF_C2H2	689	711	3.24e0	SMART
ZnF_C2H2	725	747	1.38e-3	SMART
ZnF_C2H2	753	775	5.67e-5	SMART
ZnF_C2H2	781	803	3.11e-2	SMART
ZnF_C2H2	809	831	8.34e-3	SMART
ZnF_C2H2	837	859	4.79e-3	SMART
ZnF_C2H2	865	888	4.79e-3	SMART
ZnF_C2H2	894	917	5.06e-2	SMART
low complexity region	948	959	N/A	INTRINSIC
low complexity region	1148	1170	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000119200

SMART Domains

Protein: ENSMUSP00000113002
Gene: ENSMUSG00000014039

Domain	Start	End	E-Value	Type
PDB:3RAY\|A	7	92	1e-6	PDB
Blast:SET	49	110	7e-39	BLAST

Predicted Effect

probably null
Transcript: ENSMUST00000121584
AA Change: Y158*

SMART Domains

Protein: ENSMUSP00000113791
Gene: ENSMUSG00000014039
AA Change: Y158*

Domain	Start	End	E-Value	Type
SET	49	165	5.96e-1	SMART
ZnF_C2H2	197	219	3.99e0	SMART
low complexity region	264	277	N/A	INTRINSIC
ZnF_C2H2	376	398	3.89e-3	SMART
ZnF_C2H2	408	431	2.75e-3	SMART
ZnF_C2H2	442	462	1.88e2	SMART
ZnF_C2H2	469	491	5.42e-2	SMART
ZnF_C2H2	496	518	1.36e-2	SMART
ZnF_C2H2	545	567	6.23e-2	SMART
ZnF_C2H2	572	594	2.75e-3	SMART
low complexity region	616	631	N/A	INTRINSIC
ZnF_C2H2	635	658	2.17e-1	SMART
ZnF_C2H2	663	685	3.24e0	SMART
ZnF_C2H2	699	721	1.38e-3	SMART
ZnF_C2H2	727	749	5.67e-5	SMART
ZnF_C2H2	755	777	3.11e-2	SMART
ZnF_C2H2	783	805	8.34e-3	SMART
ZnF_C2H2	811	833	4.79e-3	SMART
ZnF_C2H2	839	862	4.79e-3	SMART
ZnF_C2H2	868	891	5.06e-2	SMART
low complexity region	922	933	N/A	INTRINSIC
low complexity region	1122	1144	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000135505

Predicted Effect

probably null
Transcript: ENSMUST00000142295
AA Change: Y158*

SMART Domains

Protein: ENSMUSP00000120497
Gene: ENSMUSG00000014039
AA Change: Y158*

Domain	Start	End	E-Value	Type
SET	49	165	5.96e-1	SMART
low complexity region	230	243	N/A	INTRINSIC
ZnF_C2H2	342	364	3.89e-3	SMART
ZnF_C2H2	369	392	2.75e-3	SMART
ZnF_C2H2	403	423	1.88e2	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (86/88)

Allele List at MGI

Other mutations in this stock

Total: 88 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abl2	T	G	1: 156,450,104 (GRCm39)	N90K	possibly damaging	Het
Acox1	T	C	11: 116,069,001 (GRCm39)	T415A	possibly damaging	Het
Acy1	T	C	9: 106,314,921 (GRCm39)	M1V	probably null	Het
Akap13	G	A	7: 75,380,213 (GRCm39)	R462H	probably damaging	Het
Alpk3	C	A	7: 80,750,746 (GRCm39)	Y1505*	probably null	Het
Ankrd17	T	C	5: 90,390,902 (GRCm39)	N2256S	probably benign	Het
Ankrd22	C	A	19: 34,126,692 (GRCm39)	C46F	possibly damaging	Het
Arhgef5	A	T	6: 43,257,605 (GRCm39)	K1291*	probably null	Het
Arl9	T	A	5: 77,158,276 (GRCm39)	Y119*	probably null	Het
Asb14	T	C	14: 26,622,805 (GRCm39)	V89A	probably benign	Het
Banp	T	G	8: 122,676,588 (GRCm39)		probably null	Het
Btn2a2	T	C	13: 23,666,933 (GRCm39)	N224S	probably benign	Het
C1ra	C	T	6: 124,499,403 (GRCm39)	Q530*	probably null	Het
C2cd6	A	C	1: 59,107,844 (GRCm39)	S273A	probably benign	Het
Cd1d2	A	T	3: 86,895,583 (GRCm39)		probably null	Het
Cdc42bpb	T	C	12: 111,306,307 (GRCm39)	D132G	probably damaging	Het
Cfap45	C	A	1: 172,362,877 (GRCm39)	Y289*	probably null	Het
Chrdl2	T	C	7: 99,659,332 (GRCm39)		probably null	Het
Cilk1	A	G	9: 78,065,221 (GRCm39)	K377R	probably benign	Het
Cst5	C	T	2: 149,247,496 (GRCm39)	L71F	probably benign	Het
Dcaf11	T	C	14: 55,802,966 (GRCm39)	F292L	possibly damaging	Het
Dgcr8	A	G	16: 18,077,487 (GRCm39)	F641S	probably damaging	Het
Dnm3	G	A	1: 162,149,198 (GRCm39)		probably null	Het
Efcab3	G	T	11: 104,640,526 (GRCm39)	S1088I	probably benign	Het
Eral1	T	C	11: 77,966,219 (GRCm39)	K320E	probably damaging	Het
Eva1a	A	G	6: 82,069,002 (GRCm39)	T110A	possibly damaging	Het
Fbxo42	A	G	4: 140,926,917 (GRCm39)	D399G	possibly damaging	Het
Frs2	T	C	10: 116,910,007 (GRCm39)	T452A	possibly damaging	Het
Git2	T	A	5: 114,871,958 (GRCm39)	D542V	probably damaging	Het
Grk2	T	A	19: 4,356,063 (GRCm39)		probably benign	Het
Gsg1l2	A	G	11: 67,676,110 (GRCm39)	N158S	possibly damaging	Het
Hc	T	C	2: 34,918,063 (GRCm39)	N740S	probably benign	Het
Hectd1	T	C	12: 51,791,588 (GRCm39)		probably null	Het
Hemk1	A	G	9: 107,208,288 (GRCm39)		probably null	Het
Hormad2	G	T	11: 4,362,245 (GRCm39)	Y126*	probably null	Het
Hr	A	G	14: 70,795,652 (GRCm39)	E399G	possibly damaging	Het
Ilf3	C	A	9: 21,314,707 (GRCm39)	H780N	unknown	Het
Inpp5e	A	T	2: 26,298,161 (GRCm39)	S147T	probably benign	Het
Irag2	C	A	6: 145,119,427 (GRCm39)		probably null	Het
Jmjd6	T	C	11: 116,733,275 (GRCm39)	D134G	probably damaging	Het
Kif23	G	T	9: 61,844,457 (GRCm39)	Y120*	probably null	Het
Klk12	T	A	7: 43,422,780 (GRCm39)	Y236N	probably damaging	Het
Kmt5b	C	A	19: 3,852,799 (GRCm39)	Y186*	probably null	Het
Krtap9-5	A	G	11: 99,840,132 (GRCm39)	T278A	unknown	Het
Lca5	T	A	9: 83,305,509 (GRCm39)	D99V	probably damaging	Het
Leng9	A	G	7: 4,151,800 (GRCm39)	V292A	probably benign	Het
Lime1	A	G	2: 181,025,135 (GRCm39)	R231G	probably benign	Het
Mctp2	T	A	7: 71,861,438 (GRCm39)	E402D	probably damaging	Het
Mrpl28	T	A	17: 26,343,589 (GRCm39)	S116R	probably damaging	Het
Muc15	A	T	2: 110,561,862 (GRCm39)	R99S	probably benign	Het
Myh2	G	A	11: 67,083,368 (GRCm39)	A1444T	probably benign	Het
Mynn	T	A	3: 30,657,825 (GRCm39)	Y48N	probably damaging	Het
Myo1b	A	T	1: 51,836,639 (GRCm39)	V274E	possibly damaging	Het
Nemp1	T	A	10: 127,528,923 (GRCm39)	M209K	possibly damaging	Het
Nlrc4	G	T	17: 74,752,507 (GRCm39)	D625E	probably benign	Het
Or10al6	C	T	17: 38,082,910 (GRCm39)	A122V	probably damaging	Het
Or11a4	T	C	17: 37,536,276 (GRCm39)	F87L	probably benign	Het
Or4p4	T	C	2: 88,482,622 (GRCm39)	L42P	probably damaging	Het
Otog	T	C	7: 45,913,543 (GRCm39)	V792A	probably benign	Het
Paqr8	T	C	1: 21,005,442 (GRCm39)	Y199H	probably damaging	Het
Popdc3	A	G	10: 45,191,117 (GRCm39)	D76G	probably damaging	Het
Ppme1	T	C	7: 99,991,069 (GRCm39)	N210D	probably benign	Het
Prrg2	A	T	7: 44,709,687 (GRCm39)	L70Q	probably benign	Het
Psmg4	C	T	13: 34,361,966 (GRCm39)	R85W	probably damaging	Het
Rab11fip4	A	T	11: 79,580,478 (GRCm39)	T437S	probably benign	Het
Rap2a	T	A	14: 120,716,338 (GRCm39)	M67K	probably damaging	Het
Rnf123	A	T	9: 107,946,208 (GRCm39)	H322Q	probably benign	Het
Rxfp2	A	T	5: 149,990,801 (GRCm39)	T521S	possibly damaging	Het
Scrn2	T	G	11: 96,923,992 (GRCm39)	V292G	possibly damaging	Het
Serpina3n	C	A	12: 104,377,656 (GRCm39)	P303H	probably benign	Het
Spop	C	T	11: 95,376,727 (GRCm39)	T260M	probably damaging	Het
Surf2	G	A	2: 26,809,354 (GRCm39)	G224D	probably benign	Het
Synm	T	G	7: 67,382,971 (GRCm39)	N669T	unknown	Het
Tmprss13	T	C	9: 45,239,721 (GRCm39)	S10P	unknown	Het
Trav9d-1	T	A	14: 53,029,970 (GRCm39)	S25T	probably benign	Het
Trpc3	A	T	3: 36,678,565 (GRCm39)	I840K	probably damaging	Het
Tsbp1	T	A	17: 34,636,539 (GRCm39)		probably null	Het
Tssc4	C	A	7: 142,622,999 (GRCm39)		probably benign	Het
Ttc39d	G	T	17: 80,523,579 (GRCm39)	R79S	possibly damaging	Het
Txlnb	T	A	10: 17,675,082 (GRCm39)	S78R	probably damaging	Het
Vmn1r142	T	A	7: 21,862,784 (GRCm39)	Q226L	possibly damaging	Het
Vmn1r230	T	C	17: 21,067,146 (GRCm39)	S112P	probably damaging	Het
Wrnip1	T	C	13: 33,000,360 (GRCm39)	F456L	possibly damaging	Het
Zc3h8	G	T	2: 128,775,215 (GRCm39)	H148Q	probably benign	Het
Zcchc3	A	G	2: 152,256,615 (GRCm39)	V28A	probably benign	Het
Zdbf2	T	A	1: 63,346,669 (GRCm39)	C1683S	probably benign	Het
Zfp874a	T	C	13: 67,573,723 (GRCm39)		probably null	Het
Zmym4	A	G	4: 126,776,029 (GRCm39)	S1260P	probably benign	Het

Other mutations in Prdm15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00973:Prdm15	APN	16	97,607,367 (GRCm39)	splice site	probably benign
IGL01325:Prdm15	APN	16	97,607,717 (GRCm39)	missense	probably damaging	1.00
IGL02195:Prdm15	APN	16	97,637,029 (GRCm39)	missense	probably damaging	1.00
IGL02473:Prdm15	APN	16	97,638,805 (GRCm39)	splice site	probably null
IGL02502:Prdm15	APN	16	97,640,539 (GRCm39)	missense	probably damaging	1.00
IGL02604:Prdm15	APN	16	97,623,142 (GRCm39)	missense	probably benign
R0408:Prdm15	UTSW	16	97,636,986 (GRCm39)	missense	possibly damaging	0.92
R0437:Prdm15	UTSW	16	97,613,759 (GRCm39)	missense	probably benign	0.00
R0497:Prdm15	UTSW	16	97,595,534 (GRCm39)	missense	possibly damaging	0.63
R0590:Prdm15	UTSW	16	97,598,961 (GRCm39)	missense	possibly damaging	0.95
R0630:Prdm15	UTSW	16	97,638,907 (GRCm39)	missense	probably null	1.00
R0661:Prdm15	UTSW	16	97,630,882 (GRCm39)	missense	probably benign	0.34
R0718:Prdm15	UTSW	16	97,613,833 (GRCm39)	missense	possibly damaging	0.89
R1144:Prdm15	UTSW	16	97,609,908 (GRCm39)	missense	probably damaging	1.00
R1240:Prdm15	UTSW	16	97,638,800 (GRCm39)	missense	probably damaging	0.98
R1605:Prdm15	UTSW	16	97,640,506 (GRCm39)	missense	probably damaging	1.00
R1908:Prdm15	UTSW	16	97,638,885 (GRCm39)	missense	probably benign	0.27
R2081:Prdm15	UTSW	16	97,604,980 (GRCm39)	nonsense	probably null
R2208:Prdm15	UTSW	16	97,600,464 (GRCm39)	splice site	probably null
R3787:Prdm15	UTSW	16	97,598,945 (GRCm39)	missense	probably benign	0.00
R3890:Prdm15	UTSW	16	97,600,771 (GRCm39)	missense	probably damaging	1.00
R4326:Prdm15	UTSW	16	97,607,715 (GRCm39)	missense	probably damaging	1.00
R4728:Prdm15	UTSW	16	97,622,986 (GRCm39)	missense	probably benign	0.04
R4952:Prdm15	UTSW	16	97,607,277 (GRCm39)	missense	probably damaging	0.99
R4998:Prdm15	UTSW	16	97,595,689 (GRCm39)	missense	probably damaging	0.97
R5225:Prdm15	UTSW	16	97,609,875 (GRCm39)	missense	probably damaging	1.00
R5505:Prdm15	UTSW	16	97,618,183 (GRCm39)	missense	possibly damaging	0.76
R5628:Prdm15	UTSW	16	97,600,823 (GRCm39)	missense	probably damaging	0.98
R5721:Prdm15	UTSW	16	97,608,296 (GRCm39)	missense	possibly damaging	0.74
R5873:Prdm15	UTSW	16	97,609,889 (GRCm39)	missense	probably damaging	1.00
R5980:Prdm15	UTSW	16	97,613,770 (GRCm39)	nonsense	probably null
R6311:Prdm15	UTSW	16	97,600,255 (GRCm39)	missense	probably null	0.08
R6540:Prdm15	UTSW	16	97,637,005 (GRCm39)	missense	probably benign	0.13
R7053:Prdm15	UTSW	16	97,595,742 (GRCm39)	nonsense	probably null
R7241:Prdm15	UTSW	16	97,596,941 (GRCm39)	missense	possibly damaging	0.50
R7473:Prdm15	UTSW	16	97,623,046 (GRCm39)	missense	possibly damaging	0.68
R7762:Prdm15	UTSW	16	97,619,473 (GRCm39)	missense	probably benign	0.00
R7911:Prdm15	UTSW	16	97,613,792 (GRCm39)	missense	probably benign	0.35
R8053:Prdm15	UTSW	16	97,636,807 (GRCm39)	missense	probably benign	0.17
R8127:Prdm15	UTSW	16	97,638,910 (GRCm39)	missense	probably benign	0.24
R8213:Prdm15	UTSW	16	97,608,260 (GRCm39)	missense	probably damaging	1.00
R8708:Prdm15	UTSW	16	97,618,066 (GRCm39)	missense	unknown
R8768:Prdm15	UTSW	16	97,638,888 (GRCm39)	missense	probably benign
R9000:Prdm15	UTSW	16	97,595,470 (GRCm39)	missense	probably benign	0.03
R9513:Prdm15	UTSW	16	97,607,704 (GRCm39)	missense	probably damaging	1.00
R9583:Prdm15	UTSW	16	97,623,142 (GRCm39)	missense	probably benign
RF002:Prdm15	UTSW	16	97,600,829 (GRCm39)	missense	probably damaging	1.00
RF021:Prdm15	UTSW	16	97,609,956 (GRCm39)	missense	probably damaging	1.00
Z1177:Prdm15	UTSW	16	97,618,159 (GRCm39)	missense	possibly damaging	0.54

Predicted Primers

PCR Primer
(F):5'- AAGGGTCCCAAGATCTGGTG -3'
(R):5'- CATCCTGTGTGCTTCGACAC -3'

Sequencing Primer
(F):5'- TCCATATGCCCAGGTATGGTGAC -3'
(R):5'- GTGCTTCGACACTTCCAATGAGG -3'

Posted On

2019-10-07