Mutagenetix > Incidental Mutation

Incidental Mutation 'R7471:Zfp655'

579189

Institutional Source

Beutler Lab

Gene Symbol

Zfp655

Ensembl Gene

ENSMUSG00000007812

Gene Name

zinc finger protein 655

Synonyms

2700038I16Rik, 9030409O18Rik

MMRRC Submission

045545-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.353) question?

Stock #

R7471 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

145168525-145184112 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 145181542 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Asparagine at position 467 (Y467N)

Ref Sequence

ENSEMBL: ENSMUSP00000128969 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167316] [ENSMUST00000199322]

AlphaFold

Q9CZP3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000167316
AA Change: Y467N

PolyPhen 2 Score 0.795 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000128969
Gene: ENSMUSG00000007812
AA Change: Y467N

Domain	Start	End	E-Value	Type
ZnF_C2H2	243	265	5.29e-5	SMART
ZnF_C2H2	271	293	2.99e-4	SMART
ZnF_C2H2	299	328	1.38e2	SMART
ZnF_C2H2	334	356	6.78e-3	SMART
ZnF_C2H2	361	383	3.99e0	SMART
ZnF_C2H2	411	433	3.63e-3	SMART
ZnF_C2H2	439	461	1.01e-1	SMART
low complexity region	463	475	N/A	INTRINSIC
ZnF_C2H2	495	517	6.47e1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000199322

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

100% (73/73)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a zinc finger protein. The zinc finger proteins are involved in DNA binding and protein-protein interactions. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 72 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acaca	T	A	11: 84,168,608 (GRCm39)		probably null	Het
Ace	T	C	11: 105,864,308 (GRCm39)	V483A	probably damaging	Het
Akap13	G	A	7: 75,380,213 (GRCm39)	R462H	probably damaging	Het
Angptl2	A	T	2: 33,133,751 (GRCm39)	T358S	possibly damaging	Het
Apoh	C	T	11: 108,298,131 (GRCm39)	P144S	probably damaging	Het
Arid1a	A	G	4: 133,408,355 (GRCm39)	W1666R	unknown	Het
Asap3	A	G	4: 135,960,957 (GRCm39)	H223R	possibly damaging	Het
Bcl2l12	A	G	7: 44,645,977 (GRCm39)	S81P	possibly damaging	Het
Brd10	A	C	19: 29,707,139 (GRCm39)		probably null	Het
C8a	A	G	4: 104,674,822 (GRCm39)	S550P	probably benign	Het
Cacna2d1	C	G	5: 16,139,973 (GRCm39)		probably benign	Het
Ccdc170	A	G	10: 4,470,803 (GRCm39)	T216A	probably benign	Het
Chd7	A	T	4: 8,859,197 (GRCm39)	K2429I	probably damaging	Het
Chd8	T	C	14: 52,441,569 (GRCm39)	N2181S	probably benign	Het
Cimap1c	C	T	9: 56,759,783 (GRCm39)		probably null	Het
Clip3	T	C	7: 30,001,377 (GRCm39)	V342A	possibly damaging	Het
Cst5	C	T	2: 149,247,496 (GRCm39)	L71F	probably benign	Het
Cyp20a1	T	C	1: 60,393,799 (GRCm39)	F139S	probably damaging	Het
Dclre1b	T	A	3: 103,710,430 (GRCm39)	R494W	probably benign	Het
Dicer1	A	G	12: 104,660,969 (GRCm39)	I1695T	probably damaging	Het
Dnah7a	A	G	1: 53,458,858 (GRCm39)	L3760P	probably damaging	Het
Dock1	A	G	7: 134,765,072 (GRCm39)	D1688G	possibly damaging	Het
Drosha	G	A	15: 12,889,742 (GRCm39)	R870Q	probably damaging	Het
Dsg2	G	T	18: 20,713,675 (GRCm39)	M215I	probably benign	Het
Dst	A	T	1: 34,233,651 (GRCm39)	D3453V	possibly damaging	Het
Epha10	A	T	4: 124,796,365 (GRCm39)	Y358F		Het
Ermp1	A	T	19: 29,590,054 (GRCm39)	M853K	probably benign	Het
Hace1	T	G	10: 45,577,075 (GRCm39)	I823S	probably benign	Het
Hectd3	A	T	4: 116,853,785 (GRCm39)	Y227F	probably benign	Het
Hells	A	G	19: 38,945,501 (GRCm39)	N580S	probably benign	Het
Igkv12-44	A	G	6: 69,791,718 (GRCm39)	F82L	probably damaging	Het
Inpp5d	A	G	1: 87,623,122 (GRCm39)	D405G	probably damaging	Het
Itsn2	G	A	12: 4,758,198 (GRCm39)	R1508H	probably benign	Het
Limk1	A	T	5: 134,686,825 (GRCm39)		probably null	Het
Lingo1	T	A	9: 56,528,167 (GRCm39)	K147*	probably null	Het
Ltn1	T	C	16: 87,194,787 (GRCm39)	S1371G	probably benign	Het
Mab21l1	A	G	3: 55,691,307 (GRCm39)	D298G	possibly damaging	Het
Macf1	A	T	4: 123,366,082 (GRCm39)	V2893E	probably benign	Het
Mapk8ip1	T	C	2: 92,219,489 (GRCm39)	D114G	probably benign	Het
Med15	A	T	16: 17,540,729 (GRCm39)	W6R	probably benign	Het
Mrps34	T	C	17: 25,114,431 (GRCm39)	L97P	probably damaging	Het
Myrfl	A	T	10: 116,697,417 (GRCm39)	F36I	possibly damaging	Het
Nifk	G	A	1: 118,260,204 (GRCm39)	S253N	probably damaging	Het
Nmnat1	T	A	4: 149,557,758 (GRCm39)	T95S	probably damaging	Het
Oasl1	T	A	5: 115,073,985 (GRCm39)	I298N	probably damaging	Het
Or51a8	A	G	7: 102,549,760 (GRCm39)	Y62C	probably benign	Het
Or56b35	T	G	7: 104,963,712 (GRCm39)	L167R	probably damaging	Het
Or8g37	A	T	9: 39,731,423 (GRCm39)	M163L	probably benign	Het
Polr2b	C	T	5: 77,468,913 (GRCm39)	R230*	probably null	Het
Prkra	T	A	2: 76,477,545 (GRCm39)	E53D	probably benign	Het
Rasgrp4	T	A	7: 28,845,405 (GRCm39)	L376Q	probably damaging	Het
Rwdd3	A	G	3: 120,964,961 (GRCm39)	S65P	probably benign	Het
Satb1	G	A	17: 52,090,029 (GRCm39)	P273S	probably damaging	Het
Scg3	T	C	9: 75,589,374 (GRCm39)	E93G	probably damaging	Het
Sf3a1	T	C	11: 4,117,724 (GRCm39)	F162S	possibly damaging	Het
Sorcs1	A	G	19: 50,250,701 (GRCm39)	Y377H	probably damaging	Het
Sptbn4	G	A	7: 27,108,439 (GRCm39)	A661V	possibly damaging	Het
Stk36	A	G	1: 74,673,479 (GRCm39)	N996S	unknown	Het
Tbca	A	T	13: 94,968,923 (GRCm39)	I37F	probably damaging	Het
Tc2n	A	T	12: 101,672,716 (GRCm39)	V75E	probably damaging	Het
Tdrkh	A	T	3: 94,333,263 (GRCm39)	S243C	probably damaging	Het
Tjp1	T	C	7: 64,964,438 (GRCm39)	D911G	probably damaging	Het
Tmbim6	A	G	15: 99,299,324 (GRCm39)		probably benign	Het
Tob1	T	A	11: 94,104,708 (GRCm39)	D81E	probably benign	Het
Ttpal	T	A	2: 163,448,945 (GRCm39)		probably null	Het
Usp37	A	T	1: 74,534,787 (GRCm39)		probably null	Het
Vmn1r235	A	G	17: 21,482,658 (GRCm39)	I328V	probably benign	Het
Vmn1r28	T	A	6: 58,242,850 (GRCm39)	L231*	probably null	Het
Xirp1	G	A	9: 119,848,176 (GRCm39)	Q236*	probably null	Het
Zc3h7b	A	G	15: 81,664,682 (GRCm39)	T514A	probably damaging	Het
Zfp524	A	G	7: 5,021,200 (GRCm39)	I243V	probably benign	Het
Zfp985	A	T	4: 147,667,388 (GRCm39)	K85N	possibly damaging	Het

Other mutations in Zfp655

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00639:Zfp655	APN	5	145,180,955 (GRCm39)	missense	probably damaging	1.00
IGL01679:Zfp655	APN	5	145,180,637 (GRCm39)	missense	probably damaging	0.96
IGL02379:Zfp655	APN	5	145,180,765 (GRCm39)	missense	probably benign	0.13
IGL02647:Zfp655	APN	5	145,179,816 (GRCm39)	missense	probably benign
R0104:Zfp655	UTSW	5	145,180,825 (GRCm39)	missense	probably damaging	1.00
R0104:Zfp655	UTSW	5	145,180,825 (GRCm39)	missense	probably damaging	1.00
R0270:Zfp655	UTSW	5	145,181,267 (GRCm39)	missense	probably damaging	1.00
R0608:Zfp655	UTSW	5	145,180,867 (GRCm39)	missense	possibly damaging	0.57
R1528:Zfp655	UTSW	5	145,181,411 (GRCm39)	missense	probably damaging	0.98
R2076:Zfp655	UTSW	5	145,181,410 (GRCm39)	missense	possibly damaging	0.89
R2119:Zfp655	UTSW	5	145,181,594 (GRCm39)	missense	probably damaging	0.98
R2375:Zfp655	UTSW	5	145,181,206 (GRCm39)	missense	probably benign	0.10
R2403:Zfp655	UTSW	5	145,181,356 (GRCm39)	missense	probably benign	0.00
R4032:Zfp655	UTSW	5	145,180,858 (GRCm39)	missense	possibly damaging	0.50
R4532:Zfp655	UTSW	5	145,181,507 (GRCm39)	missense	probably benign	0.06
R4880:Zfp655	UTSW	5	145,181,168 (GRCm39)	missense	probably damaging	0.99
R5484:Zfp655	UTSW	5	145,180,445 (GRCm39)	missense	probably benign	0.01
R5529:Zfp655	UTSW	5	145,181,546 (GRCm39)	missense	probably damaging	0.96
R6193:Zfp655	UTSW	5	145,181,586 (GRCm39)	missense	probably benign	0.03
R6195:Zfp655	UTSW	5	145,180,572 (GRCm39)	missense	possibly damaging	0.52
R7050:Zfp655	UTSW	5	145,181,545 (GRCm39)	missense	probably benign	0.12
R7612:Zfp655	UTSW	5	145,173,999 (GRCm39)	missense	unknown
R7626:Zfp655	UTSW	5	145,173,917 (GRCm39)	missense	probably damaging	1.00
R7989:Zfp655	UTSW	5	145,181,380 (GRCm39)	missense	probably damaging	1.00
R8557:Zfp655	UTSW	5	145,180,835 (GRCm39)	missense	probably benign	0.37
R8805:Zfp655	UTSW	5	145,181,290 (GRCm39)	missense	probably damaging	1.00
R9602:Zfp655	UTSW	5	145,181,473 (GRCm39)	missense	probably benign	0.00
R9664:Zfp655	UTSW	5	145,180,442 (GRCm39)	missense	probably damaging	1.00
Z1176:Zfp655	UTSW	5	145,180,813 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATGATGAATGTGGGCTGGCC -3'
(R):5'- GAACTTGACTAAATGTCTCCCCAAG -3'

Sequencing Primer
(F):5'- TGGGCTGGCCTATGTTAAACAAC -3'
(R):5'- CCAAGTTCACTGCATTCATAAGG -3'

Posted On

2019-10-07