|Institutional Source||Beutler Lab|
|Gene Name||exocyst complex component 2|
|Synonyms||2410030I24Rik, Sec5l1, Sec5|
|Essential gene?||Probably essential (E-score: 0.955)|
|Stock #||R7473 (G1)|
|Chromosomal Location||30813919-30974093 bp(-) (GRCm38)|
|Type of Mutation||critical splice donor site (2 bp from exon)|
|DNA Base Change (assembly)||A to G at 30822630 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000021785 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000021785] [ENSMUST00000102946]|
|PDB Structure||RAL BINDING DOMAIN FROM SEC5 [SOLUTION NMR]|
|Coding Region Coverage||
|Validation Efficiency||96% (80/83)|
|MGI Phenotype||FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a component of the exocyst complex, a multi-protein complex essential for the polarized targeting of exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. This protein has been shown to interact with the Ral subfamily of GTPases and thereby mediate exocytosis by tethering vesicles to the plasma membrane. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]|
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Exoc2||
(F):5'- GCAACACATCATCAGGAAAGTG -3'
(R):5'- CTGCAATGCCTTTTAGAAACGAAG -3'
(F):5'- CATCATCAGGAAAGTGAGATCAC -3'
(R):5'- TGCAGAAGACATAGATTTGGTCCCC -3'