Mutagenetix > Incidental Mutation

Incidental Mutation 'R7475:Oas1b'

579401

Institutional Source

Beutler Lab

Gene Symbol

Oas1b

Ensembl Gene

ENSMUSG00000029605

Gene Name

2'-5' oligoadenylate synthetase 1B

Synonyms

Oias-2, Wnv, Flavivirus resistance, Mmu-L1, L1, Flv, Oias2

MMRRC Submission

045549-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.060) question?

Stock #

R7475 (G1)

Quality Score

178.009

Status

Validated

Chromosome

Chromosomal Location

120812635-120824163 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 120817640 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 162 (N162I)

Ref Sequence

ENSEMBL: ENSMUSP00000083564 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086377] [ENSMUST00000183291]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000086377
AA Change: N162I

PolyPhen 2 Score 0.993 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000083564
Gene: ENSMUSG00000029605
AA Change: N162I

Domain	Start	End	E-Value	Type
Pfam:OAS1_C	158	251	7.1e-37	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000183291
AA Change: N162I

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000138088
Gene: ENSMUSG00000029605
AA Change: N162I

Domain	Start	End	E-Value	Type
Pfam:OAS1_C	158	345	3.4e-79	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

99% (77/78)

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc5	G	T	16: 20,399,989 (GRCm38)	N214K	probably benign	Het
Abcf1	A	G	17: 35,963,567 (GRCm38)		probably null	Het
Agxt2	A	T	15: 10,409,537 (GRCm38)	M508L	probably benign	Het
Akr1c21	A	T	13: 4,576,319 (GRCm38)	Y114F	probably benign	Het
Amz1	T	C	5: 140,744,186 (GRCm38)		probably null	Het
Ank1	G	T	8: 23,132,630 (GRCm38)	A1732S	probably benign	Het
Atg16l1	T	C	1: 87,760,083 (GRCm38)	S50P	possibly damaging	Het
AW551984	A	G	9: 39,597,940 (GRCm38)	S302P	probably damaging	Het
Ces3a	T	A	8: 105,053,690 (GRCm38)		probably null	Het
Dedd	C	A	1: 171,340,313 (GRCm38)	P185Q	probably benign	Het
Fam186a	A	T	15: 99,947,514 (GRCm38)	V283E	unknown	Het
Fat2	C	A	11: 55,303,653 (GRCm38)	V1187F	probably benign	Het
Fbxw11	T	C	11: 32,711,999 (GRCm38)		probably null	Het
Fcgbp	C	T	7: 28,102,976 (GRCm38)	T1443I	probably damaging	Het
Foxj2	A	G	6: 122,837,842 (GRCm38)	D279G	probably benign	Het
Gbp5	A	G	3: 142,501,361 (GRCm38)	D97G	probably damaging	Het
Gm49368	A	T	7: 128,107,982 (GRCm38)	T661S	possibly damaging	Het
Gria4	T	G	9: 4,513,330 (GRCm38)	T260P	probably damaging	Het
Gtf2ird2	T	A	5: 134,201,426 (GRCm38)	D195E	possibly damaging	Het
Hectd4	T	A	5: 121,358,133 (GRCm38)		probably null	Het
Ifngr2	G	A	16: 91,557,909 (GRCm38)	C32Y	unknown	Het
Ikzf5	A	T	7: 131,392,059 (GRCm38)	C280S	probably benign	Het
Ints9	G	T	14: 65,026,465 (GRCm38)	E395D	probably null	Het
Isoc2b	T	C	7: 4,851,085 (GRCm38)	D96G	probably benign	Het
Jmjd1c	T	G	10: 67,225,313 (GRCm38)	S967R	probably benign	Het
Kcnj3	A	T	2: 55,437,326 (GRCm38)	K42N	probably benign	Het
Kiz	T	C	2: 146,891,086 (GRCm38)	V394A	possibly damaging	Het
Knl1	A	T	2: 119,087,546 (GRCm38)	H1795L	probably damaging	Het
Lmntd2	A	G	7: 141,210,689 (GRCm38)		probably null	Het
Loxhd1	C	A	18: 77,412,305 (GRCm38)	D1690E	possibly damaging	Het
Lrp1b	T	C	2: 41,344,576 (GRCm38)	D1121G		Het
Map3k2	T	C	18: 32,199,962 (GRCm38)	V63A	possibly damaging	Het
Mcc	G	T	18: 44,476,236 (GRCm38)	A499D	probably damaging	Het
Mcpt9	T	A	14: 56,026,943 (GRCm38)	I232F	probably damaging	Het
Meltf	A	G	16: 31,881,938 (GRCm38)	K92R	probably benign	Het
Mff	T	A	1: 82,745,438 (GRCm38)		probably null	Het
Mrgpra3	T	A	7: 47,589,947 (GRCm38)	Y77F	probably damaging	Het
Mylk	G	A	16: 34,914,076 (GRCm38)		probably null	Het
Ndufv2	A	G	17: 66,087,537 (GRCm38)	V111A	possibly damaging	Het
Nkd2	T	C	13: 73,825,742 (GRCm38)	E99G	probably damaging	Het
Nlk	C	A	11: 78,583,399 (GRCm38)	G358V	probably damaging	Het
Nnmt	A	T	9: 48,592,232 (GRCm38)	C165S	probably damaging	Het
Nxpe4	T	A	9: 48,393,340 (GRCm38)	C242*	probably null	Het
Olfr1191-ps1	A	G	2: 88,643,210 (GRCm38)	I148V	probably benign	Het
Otog	A	G	7: 46,267,276 (GRCm38)	N879S	probably damaging	Het
Park2	A	G	17: 11,434,614 (GRCm38)	D199G	probably benign	Het
Pcsk7	T	A	9: 45,927,625 (GRCm38)	Y612N	probably damaging	Het
Peg10	CCACATCAGGATCCACATCAGGATGCACATCAGCATCAGGATCCCCATCAGGATGCACATCAGGATCCACATCAGGATGCACATCAG	CCACATCAGGATCCACATCAGGATGCACATCAG	6: 4,756,398 (GRCm38)		probably benign	Het
Pgbd5	T	C	8: 124,434,011 (GRCm38)	D39G	probably benign	Het
Pkhd1l1	C	T	15: 44,505,185 (GRCm38)	Q800*	probably null	Het
Pkn3	T	C	2: 30,087,110 (GRCm38)	S621P	probably benign	Het
Polr3c	T	C	3: 96,715,185 (GRCm38)	I385V	probably benign	Het
Ppp1r21	G	T	17: 88,555,603 (GRCm38)	G257W	probably benign	Het
Pxylp1	C	T	9: 96,856,367 (GRCm38)		probably null	Het
Rasgrp1	T	C	2: 117,286,108 (GRCm38)	T613A	probably benign	Het
Robo3	C	T	9: 37,425,378 (GRCm38)	V387I	probably benign	Het
Rxfp2	T	A	5: 150,049,581 (GRCm38)	Y174N	possibly damaging	Het
Sec24a	T	C	11: 51,713,552 (GRCm38)	M746V	probably damaging	Het
Sema4f	T	A	6: 82,914,374 (GRCm38)	E571D	possibly damaging	Het
Sept3	T	C	15: 82,286,456 (GRCm38)	V217A	probably benign	Het
Serpinb1b	A	C	13: 33,093,565 (GRCm38)	K260N	probably benign	Het
Sin3b	A	G	8: 72,749,872 (GRCm38)	T645A	possibly damaging	Het
Sobp	C	T	10: 43,021,834 (GRCm38)	R585Q	probably damaging	Het
Specc1l	T	A	10: 75,246,447 (GRCm38)	L559Q	possibly damaging	Het
Srxn1	C	T	2: 152,105,653 (GRCm38)		probably benign	Het
Sspo	G	A	6: 48,455,860 (GRCm38)	R890Q	probably benign	Het
Stard9	A	G	2: 120,688,110 (GRCm38)	D505G	probably damaging	Het
Tjp1	A	T	7: 65,322,339 (GRCm38)	I653K	probably damaging	Het
Tnks	C	T	8: 34,831,712 (GRCm38)	E1296K	probably damaging	Het
Ttbk2	A	T	2: 120,748,640 (GRCm38)	I667N	probably benign	Het
Usp24	T	C	4: 106,342,353 (GRCm38)	S165P	possibly damaging	Het
Usp46	T	A	5: 74,028,937 (GRCm38)	K109*	probably null	Het
Vmn1r191	A	G	13: 22,178,772 (GRCm38)	C271R	probably benign	Het
Wisp3	T	A	10: 39,158,300 (GRCm38)	Y102F	probably damaging	Het
Zfp592	T	C	7: 81,023,452 (GRCm38)	S55P	probably damaging	Het
Zmynd15	T	C	11: 70,461,041 (GRCm38)	S158P	probably benign	Het
Zscan22	T	G	7: 12,906,737 (GRCm38)	C303G	probably damaging	Het

Other mutations in Oas1b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R4526:Oas1b	UTSW	5	120,822,102 (GRCm38)	splice site	probably null
R4783:Oas1b	UTSW	5	120,814,513 (GRCm38)	missense	probably benign	0.26
R5344:Oas1b	UTSW	5	120,822,204 (GRCm38)	missense	probably benign	0.00
R6565:Oas1b	UTSW	5	120,814,546 (GRCm38)	missense	possibly damaging	0.71
R7185:Oas1b	UTSW	5	120,817,772 (GRCm38)	missense	not run
R7450:Oas1b	UTSW	5	120,821,256 (GRCm38)	nonsense	probably null
R7640:Oas1b	UTSW	5	120,821,414 (GRCm38)	missense	probably damaging	1.00
R9425:Oas1b	UTSW	5	120,817,628 (GRCm38)	missense	probably benign	0.00
R9712:Oas1b	UTSW	5	120,814,485 (GRCm38)	missense	probably damaging	1.00
Z1189:Oas1b	UTSW	5	120,817,778 (GRCm38)	frame shift	probably null

Predicted Primers

PCR Primer
(F):5'- ACTGGGAGCAGGGACTTTGTAG -3'
(R):5'- GCCAATGCCTTACCAGTTGG -3'

Sequencing Primer
(F):5'- CAGGGACTTTGTAGTGCACAGC -3'
(R):5'- CAGTTGGTACCAGTGCGTGAC -3'

Posted On

2019-10-07