|Institutional Source||Beutler Lab|
|Gene Name||mitogen-activated protein kinase kinase kinase 2|
|Synonyms||MEK kinase 2, Mekk2, 9630061B06Rik|
|Essential gene?||Non essential (E-score: 0.000)|
|Stock #||R7475 (G1)|
|Chromosomal Location||32163089-32236751 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 32199962 bp (GRCm38)|
|Amino Acid Change||Valine to Alanine at position 63 (V63A)|
|Ref Sequence||ENSEMBL: ENSMUSP00000094326 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000096575]|
|AlphaFold||no structure available at present|
AA Change: V63A
PolyPhen 2 Score 0.608 (Sensitivity: 0.87; Specificity: 0.91)
AA Change: V63A
|Meta Mutation Damage Score||0.6436|
|Coding Region Coverage||
|Validation Efficiency||99% (77/78)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of serine/threonine protein kinase family. This kinase preferentially activates other kinases involved in the MAP kinase signaling pathway. This kinase has been shown to directly phosphorylate and activate Ikappa B kinases, and thus plays a role in NF-kappa B signaling pathway. This kinase has also been found to bind and activate protein kinase C-related kinase 2, which suggests its involvement in a regulated signaling process. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for disruptions in this gene are grossly normal and fertile. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Map3k2||
(F):5'- GCAGTCTATTACACACATGCACAG -3'
(R):5'- TGTGCATCTCACTCCTATAATGTAC -3'
(F):5'- GATTTAAACACTCTCCTAAGTAGCAC -3'
(R):5'- GGACTACTAAAAATAGTATGCGT -3'