Mutagenetix > Incidental Mutation

Incidental Mutation 'R7476:Vmn1r175'

579491

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r175

Ensembl Gene

ENSMUSG00000095632

Gene Name

vomeronasal 1 receptor 175

Synonyms

Gm6299

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R7476 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

23807182-23810643 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 23808422 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 260 (N260S)

Ref Sequence

ENSEMBL: ENSMUSP00000126357 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000166141] [ENSMUST00000228383]

AlphaFold

K7N6T9

Predicted Effect

probably benign
Transcript: ENSMUST00000166141
AA Change: N260S

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000126357
Gene: ENSMUSG00000095632
AA Change: N260S

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	296	8.8e-15	PFAM
Pfam:7tm_1	13	285	2.5e-6	PFAM
Pfam:V1R	41	295	4.4e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000228383
AA Change: N260S

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
5730596B20Rik	C	T	6: 52,179,034	P27S	unknown	Het
Abi3bp	A	T	16: 56,614,746	K602*	probably null	Het
Adam29	G	T	8: 55,873,195	H75N	probably damaging	Het
Ajap1	C	T	4: 153,384,855	E373K	probably damaging	Het
Alg6	C	A	4: 99,743,876	H206N	probably damaging	Het
Alox5	A	T	6: 116,415,433	S376T	probably benign	Het
Arhgap19	A	T	19: 41,782,363	Y321N	probably benign	Het
Brip1	A	G	11: 86,157,808	V236A	probably benign	Het
C1ra	C	T	6: 124,522,699	P615S	probably damaging	Het
Ccdc69	C	T	11: 55,051,198	V161I	possibly damaging	Het
Chil5	G	A	3: 106,020,007	R163C	possibly damaging	Het
Cldnd1	A	G	16: 58,729,544	D30G	probably damaging	Het
Colca2	G	A	9: 51,277,600	A19V	possibly damaging	Het
Csmd1	A	G	8: 15,895,731	C3561R	probably damaging	Het
Daxx	T	G	17: 33,911,281	V90G	probably damaging	Het
Dgkg	T	A	16: 22,622,304		probably benign	Het
Dmrta2	A	G	4: 109,982,025	H323R	probably damaging	Het
Dysf	A	T	6: 84,064,896	T161S	probably benign	Het
Fat1	G	T	8: 45,031,274	R3183L	probably benign	Het
Fcho1	C	A	8: 71,713,546	D347Y	probably damaging	Het
Gm21798	T	C	15: 64,817,706	C5R	unknown	Het
Hmcn1	T	C	1: 150,580,267	R5301G	probably damaging	Het
Hoxd11	T	C	2: 74,684,115	F330L	probably damaging	Het
Lactbl1	A	G	4: 136,637,639	D434G	probably benign	Het
Lgals8	A	T	13: 12,448,481	N191K	probably damaging	Het
Lmod2	T	A	6: 24,597,921	Y13*	probably null	Het
Malrd1	T	C	2: 16,142,304	S1986P	unknown	Het
Map3k9	C	T	12: 81,743,808	D324N	probably damaging	Het
Mcmbp	C	A	7: 128,703,582	K487N	probably damaging	Het
Mrpl4	G	T	9: 21,002,771		probably benign	Het
Nectin2	T	C	7: 19,717,621	D496G	possibly damaging	Het
Nom1	T	C	5: 29,442,536	S590P	probably benign	Het
Noto	T	C	6: 85,425,499	F156L	probably damaging	Het
Nrf1	T	A	6: 30,116,272	D314E	probably damaging	Het
Nup93	T	C	8: 94,303,632	L373P	probably damaging	Het
Olfr1002	T	A	2: 85,648,168	Q51L	not run	Het
Olfr1168	A	T	2: 88,184,966	M30L	probably benign	Het
Olfr1205	A	G	2: 88,831,588	Q157R	probably benign	Het
Olfr1239	T	C	2: 89,417,499	K305E	possibly damaging	Het
Olfr1369-ps1	A	C	13: 21,116,021	I110L	probably benign	Het
Olfr1412	A	C	1: 92,589,264	R311S	probably benign	Het
Pclo	A	T	5: 14,521,331	K243N	probably damaging	Het
Pdgfra	T	A	5: 75,170,603	C290S	probably damaging	Het
Pnlip	G	A	19: 58,679,634		probably null	Het
Ptprm	A	T	17: 66,725,791	H1022Q	probably benign	Het
Rfx2	T	C	17: 56,803,527	D153G	probably benign	Het
Rps6ka2	T	C	17: 7,271,633	F317L	probably damaging	Het
Rtl1	T	A	12: 109,591,105	E1433D	unknown	Het
Sarnp	A	G	10: 128,833,354	T27A	probably benign	Het
Shc3	A	T	13: 51,448,006	M295K	probably benign	Het
Slc1a3	T	A	15: 8,643,084	M304L	probably damaging	Het
Slco1a6	G	T	6: 142,103,001	T351K	possibly damaging	Het
Snrk	A	G	9: 122,157,222	N219S	probably damaging	Het
Spats2	T	A	15: 99,212,141	V473E	probably benign	Het
Stk35	T	C	2: 129,810,725	L382P	probably damaging	Het
Tbx10	T	C	19: 3,999,034	V315A	probably benign	Het
Tdpoz1	A	G	3: 93,670,775	L234P	probably damaging	Het
Ttn	T	G	2: 76,714,145	E32832D	probably benign	Het
Ttn	T	C	2: 76,771,186	Y18678C	probably damaging	Het
Ugt2a2	A	T	5: 87,474,494	M205K	probably damaging	Het
Utrn	C	A	10: 12,640,951	V2300L	probably benign	Het
Vmn1r63	T	A	7: 5,803,001	I211L	probably benign	Het
Vmn1r83	A	G	7: 12,321,615	W172R	possibly damaging	Het
Xirp2	T	C	2: 67,510,634	L1073P	probably benign	Het
Zfp82	A	G	7: 30,056,172	V495A	possibly damaging	Het

Other mutations in Vmn1r175

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02944:Vmn1r175	APN	7	23809166	missense	probably damaging	0.97
PIT4531001:Vmn1r175	UTSW	7	23809178	missense	possibly damaging	0.62
R1163:Vmn1r175	UTSW	7	23808512	missense	probably benign	0.30
R1901:Vmn1r175	UTSW	7	23808793	missense	probably benign	0.00
R1926:Vmn1r175	UTSW	7	23809041	missense	possibly damaging	0.81
R1957:Vmn1r175	UTSW	7	23808383	missense	probably benign	0.21
R2163:Vmn1r175	UTSW	7	23808927	missense	probably benign	0.29
R2381:Vmn1r175	UTSW	7	23808668	missense	probably benign	0.31
R3109:Vmn1r175	UTSW	7	23808968	missense	probably benign	0.00
R3419:Vmn1r175	UTSW	7	23808650	missense	probably damaging	0.96
R3941:Vmn1r175	UTSW	7	23808968	missense	probably benign	0.00
R4982:Vmn1r175	UTSW	7	23809069	missense	possibly damaging	0.61
R5191:Vmn1r175	UTSW	7	23809022	missense	possibly damaging	0.91
R5326:Vmn1r175	UTSW	7	23809106	missense	possibly damaging	0.88
R5333:Vmn1r175	UTSW	7	23808579	missense	possibly damaging	0.69
R5476:Vmn1r175	UTSW	7	23809131	missense	possibly damaging	0.82
R5542:Vmn1r175	UTSW	7	23809106	missense	possibly damaging	0.88
R5761:Vmn1r175	UTSW	7	23808480	missense	probably benign	0.01
R5918:Vmn1r175	UTSW	7	23808947	missense	probably damaging	1.00
R6163:Vmn1r175	UTSW	7	23809166	missense	possibly damaging	0.71
R6563:Vmn1r175	UTSW	7	23808605	missense	possibly damaging	0.61
R7616:Vmn1r175	UTSW	7	23808606	missense	possibly damaging	0.79
R8100:Vmn1r175	UTSW	7	23808587	missense	probably damaging	1.00
R8157:Vmn1r175	UTSW	7	23809098	missense	probably benign	0.00
R8699:Vmn1r175	UTSW	7	23808809	missense	probably benign	0.01
R8744:Vmn1r175	UTSW	7	23808978	missense	probably benign	0.00
R9595:Vmn1r175	UTSW	7	23809083	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GTCGGCCAAGAAACATGTTAAATG -3'
(R):5'- TCTGGAGCAGTGTCTCCATG -3'

Sequencing Primer
(F):5'- CCCAGAAAATAGAACATTTATGGCTG -3'
(R):5'- TGGTGATTCTCCTGAACAGACAC -3'

Posted On

2019-10-07