Mutagenetix > Incidental Mutation

Incidental Mutation 'R7479:Zfp945'

579700

Institutional Source

Beutler Lab

Gene Symbol

Zfp945

Ensembl Gene

ENSMUSG00000059142

Gene Name

zinc finger protein 945

Synonyms

C730040L01Rik, A630033E08Rik

MMRRC Submission

045553-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.063) question?

Stock #

R7479 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

23065671-23086108 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 23070340 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 541 (C541S)

Ref Sequence

ENSEMBL: ENSMUSP00000124123 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088696] [ENSMUST00000150092] [ENSMUST00000160457]

AlphaFold

F6WAU7

Predicted Effect

probably damaging
Transcript: ENSMUST00000088696
AA Change: C520S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000086074
Gene: ENSMUSG00000059142
AA Change: C520S

Domain	Start	End	E-Value	Type
KRAB	13	68	9.12e-18	SMART
ZnF_C2H2	210	232	1.45e-2	SMART
ZnF_C2H2	238	260	1.03e-2	SMART
ZnF_C2H2	266	288	1.76e-1	SMART
ZnF_C2H2	322	344	7.37e-4	SMART
ZnF_C2H2	350	372	1.5e-4	SMART
ZnF_C2H2	378	400	5.14e-3	SMART
ZnF_C2H2	406	428	9.73e-4	SMART
ZnF_C2H2	434	456	2.99e-4	SMART
ZnF_C2H2	462	484	4.54e-4	SMART
ZnF_C2H2	490	512	2.4e-3	SMART
ZnF_C2H2	518	540	1.38e-3	SMART
ZnF_C2H2	546	568	1.13e-4	SMART
ZnF_C2H2	574	596	5.59e-4	SMART
ZnF_C2H2	602	624	9.31e1	SMART
ZnF_C2H2	630	652	7.9e-4	SMART
ZnF_C2H2	658	680	3.69e-4	SMART
ZnF_C2H2	686	708	3.16e-3	SMART
ZnF_C2H2	714	736	2.95e-3	SMART
ZnF_C2H2	742	764	1.98e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000150092

SMART Domains

Protein: ENSMUSP00000117978
Gene: ENSMUSG00000059142

Domain	Start	End	E-Value	Type
KRAB	34	89	9.12e-18	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000160457
AA Change: C541S

PolyPhen 2 Score 0.934 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000124123
Gene: ENSMUSG00000059142
AA Change: C541S

Domain	Start	End	E-Value	Type
KRAB	34	89	9.12e-18	SMART
ZnF_C2H2	231	253	1.45e-2	SMART
ZnF_C2H2	259	281	1.03e-2	SMART
ZnF_C2H2	287	309	1.76e-1	SMART
ZnF_C2H2	343	365	7.37e-4	SMART
ZnF_C2H2	371	393	1.5e-4	SMART
ZnF_C2H2	399	421	5.14e-3	SMART
ZnF_C2H2	427	449	9.73e-4	SMART
ZnF_C2H2	455	477	2.99e-4	SMART
ZnF_C2H2	483	505	4.54e-4	SMART
ZnF_C2H2	511	533	2.4e-3	SMART
ZnF_C2H2	539	561	1.38e-3	SMART
ZnF_C2H2	567	589	1.13e-4	SMART
ZnF_C2H2	595	617	5.59e-4	SMART
ZnF_C2H2	623	645	9.31e1	SMART
ZnF_C2H2	651	673	7.9e-4	SMART
ZnF_C2H2	679	701	3.69e-4	SMART
ZnF_C2H2	707	729	3.16e-3	SMART
ZnF_C2H2	735	757	2.95e-3	SMART
ZnF_C2H2	763	785	1.98e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700003H04Rik	T	A	3: 124,372,142 (GRCm39)	M81L	probably benign	Het
Ano9	T	C	7: 140,682,348 (GRCm39)	T667A	probably damaging	Het
Anpep	T	A	7: 79,485,118 (GRCm39)	I623F	probably benign	Het
Apba2	T	C	7: 64,389,607 (GRCm39)	I501T	possibly damaging	Het
Ascc3	T	A	10: 50,525,895 (GRCm39)	Y536N	probably damaging	Het
B4galt1	T	C	4: 40,823,587 (GRCm39)	Y168C	probably damaging	Het
C1ra	G	A	6: 124,494,684 (GRCm39)	E316K	probably benign	Het
C2	A	G	17: 35,082,441 (GRCm39)	C647R	probably damaging	Het
Cnot4	G	A	6: 35,001,083 (GRCm39)	T604I	probably benign	Het
Col11a1	C	T	3: 113,896,218 (GRCm39)	T506I	unknown	Het
Cr2	A	C	1: 194,840,718 (GRCm39)		probably null	Het
Ctsm	A	T	13: 61,685,569 (GRCm39)	V281D	probably damaging	Het
Cyp2c69	T	C	19: 39,870,001 (GRCm39)	I74V	probably benign	Het
Dennd4c	T	A	4: 86,717,590 (GRCm39)	V529D	probably damaging	Het
Dlgap3	C	A	4: 127,088,418 (GRCm39)	H5N	possibly damaging	Het
Dusp10	C	A	1: 183,769,617 (GRCm39)	H194Q	probably damaging	Het
Eln	C	G	5: 134,736,429 (GRCm39)	G753A	unknown	Het
Emb	T	A	13: 117,385,962 (GRCm39)	N118K	possibly damaging	Het
Fam184a	C	T	10: 53,531,110 (GRCm39)	V755I	probably benign	Het
Fryl	A	G	5: 73,254,904 (GRCm39)	I846T	possibly damaging	Het
Gabbr2	T	A	4: 46,681,166 (GRCm39)	I722F	probably damaging	Het
Gabrb3	T	A	7: 57,474,171 (GRCm39)	D362E	possibly damaging	Het
Galnt2	G	A	8: 125,061,077 (GRCm39)	G357D	probably damaging	Het
Gbx2	A	T	1: 89,858,373 (GRCm39)	S35R	probably benign	Het
Glg1	A	G	8: 111,924,367 (GRCm39)	I207T	possibly damaging	Het
Gm10192	A	T	4: 97,071,272 (GRCm39)	N44K	unknown	Het
Gpd1	A	G	15: 99,617,984 (GRCm39)	D123G	probably benign	Het
Grm2	A	T	9: 106,531,050 (GRCm39)	D146E	possibly damaging	Het
Gsg1l2	A	G	11: 67,676,032 (GRCm39)	D132G	probably benign	Het
Hecw1	C	T	13: 14,515,425 (GRCm39)	G236R	probably damaging	Het
Hif3a	G	A	7: 16,776,560 (GRCm39)	T462I	possibly damaging	Het
Hspg2	C	T	4: 137,266,714 (GRCm39)	A1934V	probably benign	Het
Il7r	C	T	15: 9,513,117 (GRCm39)	A131T	probably damaging	Het
Itga6	C	T	2: 71,668,680 (GRCm39)	R540*	probably null	Het
Kcnh2	A	G	5: 24,530,490 (GRCm39)		probably null	Het
Kcnq4	C	T	4: 120,573,022 (GRCm39)	A260T	probably damaging	Het
Lrp1b	T	C	2: 40,691,517 (GRCm39)	N3434S		Het
Lrrc41	C	T	4: 115,946,238 (GRCm39)	P318S	probably damaging	Het
Map4	G	A	9: 109,897,892 (GRCm39)	G873R	possibly damaging	Het
Med24	A	G	11: 98,595,787 (GRCm39)	I968T	possibly damaging	Het
Mfap5	T	C	6: 122,503,821 (GRCm39)		probably null	Het
Mtcl1	A	G	17: 66,686,485 (GRCm39)	V807A	probably benign	Het
Mug1	T	A	6: 121,855,467 (GRCm39)	S934T	possibly damaging	Het
Nckap5l	C	A	15: 99,321,127 (GRCm39)	V1218F	probably damaging	Het
Nek1	T	A	8: 61,583,179 (GRCm39)	D1272E	probably benign	Het
Nkx2-4	T	C	2: 146,926,088 (GRCm39)	E258G	probably benign	Het
Polr1a	T	A	6: 71,913,281 (GRCm39)	V545E	probably damaging	Het
Ppp1r9b	A	G	11: 94,882,858 (GRCm39)	D162G	possibly damaging	Het
Rhot2	A	G	17: 26,059,723 (GRCm39)	L367P	probably damaging	Het
Ripk2	A	G	4: 16,155,154 (GRCm39)	F122L	probably benign	Het
Scn11a	T	C	9: 119,588,941 (GRCm39)	T1322A	probably benign	Het
Scn8a	T	A	15: 100,853,358 (GRCm39)	L115Q	probably damaging	Het
Sel1l3	G	T	5: 53,274,462 (GRCm39)	P1006Q	probably damaging	Het
Septin11	A	T	5: 93,304,804 (GRCm39)	N207I	probably damaging	Het
Sez6l2	C	T	7: 126,562,831 (GRCm39)	T669I	probably damaging	Het
Sfxn4	T	A	19: 60,847,112 (GRCm39)	D57V	possibly damaging	Het
Smarca2	T	A	19: 26,617,887 (GRCm39)	V306D	probably benign	Het
Srgap3	C	T	6: 112,712,794 (GRCm39)		probably null	Het
Tas2r134	T	C	2: 51,517,541 (GRCm39)	F7L	not run	Het
Tbcd	T	C	11: 121,383,431 (GRCm39)		probably null	Het
Tcn2	G	A	11: 3,867,703 (GRCm39)	A413V	probably damaging	Het
Tdp1	T	C	12: 99,857,654 (GRCm39)	V71A	probably benign	Het
Tjp1	T	C	7: 64,950,928 (GRCm39)	T1649A	probably damaging	Het
Tnc	T	C	4: 63,935,865 (GRCm39)	E357G	possibly damaging	Het
Tnrc6b	A	G	15: 80,773,327 (GRCm39)	T1158A	probably benign	Het
Ttn	C	A	2: 76,568,952 (GRCm39)	E27314*	probably null	Het
Vps35	G	T	8: 85,997,434 (GRCm39)	T512K	probably benign	Het
Zfp40	A	T	17: 23,396,292 (GRCm39)	S98R	probably benign	Het
Zfp764l1	C	T	7: 126,992,496 (GRCm39)	C38Y	probably null	Het
Zfp976	T	A	7: 42,262,603 (GRCm39)	E412D	probably benign	Het

Other mutations in Zfp945

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00754:Zfp945	APN	17	23,070,931 (GRCm39)	unclassified	probably benign
IGL01077:Zfp945	APN	17	23,071,359 (GRCm39)	missense	probably damaging	0.99
IGL02706:Zfp945	APN	17	23,076,256 (GRCm39)	missense	probably damaging	1.00
IGL03408:Zfp945	APN	17	23,071,511 (GRCm39)	nonsense	probably null
Small-scale	UTSW	17	23,069,955 (GRCm39)	missense	probably damaging	1.00
R0427:Zfp945	UTSW	17	23,084,226 (GRCm39)	missense	probably benign	0.10
R0718:Zfp945	UTSW	17	23,070,004 (GRCm39)	missense	probably damaging	1.00
R1305:Zfp945	UTSW	17	23,071,360 (GRCm39)	missense	probably damaging	0.97
R1801:Zfp945	UTSW	17	23,070,736 (GRCm39)	missense	probably damaging	0.97
R1837:Zfp945	UTSW	17	23,070,247 (GRCm39)	missense	probably damaging	1.00
R2001:Zfp945	UTSW	17	23,076,223 (GRCm39)	critical splice donor site	probably null
R4193:Zfp945	UTSW	17	23,070,144 (GRCm39)	unclassified	probably benign
R4247:Zfp945	UTSW	17	23,069,583 (GRCm39)	missense	probably damaging	0.98
R5026:Zfp945	UTSW	17	23,069,859 (GRCm39)	missense	probably damaging	1.00
R5918:Zfp945	UTSW	17	23,069,955 (GRCm39)	missense	probably damaging	1.00
R6076:Zfp945	UTSW	17	23,070,432 (GRCm39)	missense	probably damaging	1.00
R6664:Zfp945	UTSW	17	23,071,339 (GRCm39)	missense	probably damaging	0.99
R6997:Zfp945	UTSW	17	23,071,543 (GRCm39)	nonsense	probably null
R7040:Zfp945	UTSW	17	23,071,264 (GRCm39)	missense	probably damaging	0.98
R7566:Zfp945	UTSW	17	23,070,727 (GRCm39)	missense	possibly damaging	0.71
R7948:Zfp945	UTSW	17	23,071,096 (GRCm39)	missense	unknown
R8383:Zfp945	UTSW	17	23,070,798 (GRCm39)	missense	probably benign	0.05
R9776:Zfp945	UTSW	17	23,070,582 (GRCm39)	missense	possibly damaging	0.93
R9790:Zfp945	UTSW	17	23,071,228 (GRCm39)	missense	probably benign	0.33
R9791:Zfp945	UTSW	17	23,071,228 (GRCm39)	missense	probably benign	0.33
X0063:Zfp945	UTSW	17	23,071,202 (GRCm39)	unclassified	probably benign

Predicted Primers

PCR Primer
(F):5'- GATTTGTCACATTCCCTGCATT -3'
(R):5'- CTGTGACAAATCCTTCCACCGG -3'

Sequencing Primer
(F):5'- CCGGTTAAAGGATTTGTCACATCCAC -3'
(R):5'- GGAAAGACCACCTTAGAATTCATCAG -3'

Posted On

2019-10-07