Mutagenetix > Incidental Mutation

Incidental Mutation 'R7483:Scn9a'

579886

Institutional Source

Beutler Lab

Gene Symbol

Scn9a

Ensembl Gene

ENSMUSG00000075316

Gene Name

sodium channel, voltage-gated, type IX, alpha

Synonyms

PN1

MMRRC Submission

045557-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7483 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

66480080-66634962 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 66533348 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 862 (V862A)

Ref Sequence

ENSEMBL: ENSMUSP00000097642 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100063] [ENSMUST00000100064] [ENSMUST00000112354] [ENSMUST00000164384] [ENSMUST00000169900]

AlphaFold

Q62205

Predicted Effect

probably damaging
Transcript: ENSMUST00000100063
AA Change: V853A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000097641
Gene: ENSMUSG00000075316
AA Change: V853A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	403	9.5e-78	PFAM
coiled coil region	404	442	N/A	INTRINSIC
Pfam:DUF3451	465	685	1.3e-62	PFAM
Pfam:Ion_trans	768	957	9.9e-48	PFAM
Pfam:Na_trans_assoc	972	1191	2.9e-72	PFAM
low complexity region	1203	1214	N/A	INTRINSIC
Pfam:Ion_trans	1217	1445	2.8e-55	PFAM
PDB:1BYY\|A	1447	1499	9e-27	PDB
Pfam:Ion_trans	1538	1748	3.4e-52	PFAM
Pfam:PKD_channel	1599	1755	1.1e-7	PFAM
IQ	1877	1899	1.03e-3	SMART
low complexity region	1956	1972	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000100064
AA Change: V862A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000097642
Gene: ENSMUSG00000075316
AA Change: V862A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	125	412	2.2e-84	PFAM
low complexity region	433	446	N/A	INTRINSIC
Pfam:Na_trans_cytopl	483	693	7.5e-76	PFAM
Pfam:Ion_trans	742	977	4.1e-57	PFAM
Pfam:Na_trans_assoc	981	1185	1.4e-58	PFAM
Pfam:Ion_trans	1189	1466	7e-67	PFAM
Pfam:Ion_trans	1512	1769	1e-55	PFAM
Pfam:PKD_channel	1605	1763	2.6e-7	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000112354
AA Change: V851A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000107973
Gene: ENSMUSG00000075316
AA Change: V851A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.2e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000164384
AA Change: V862A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000126528
Gene: ENSMUSG00000075316
AA Change: V862A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	1.1e-77	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	694	4.2e-66	PFAM
Pfam:Ion_trans	777	966	8.8e-48	PFAM
Pfam:Na_trans_assoc	981	1200	6e-72	PFAM
low complexity region	1212	1223	N/A	INTRINSIC
Pfam:Ion_trans	1226	1454	2.5e-55	PFAM
PDB:1BYY\|A	1456	1508	6e-29	PDB
Pfam:Ion_trans	1547	1757	3e-52	PFAM
Pfam:PKD_channel	1608	1764	8.1e-8	PFAM
IQ	1886	1908	1.03e-3	SMART
low complexity region	1965	1981	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000169900
AA Change: V851A

PolyPhen 2 Score 0.986 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000131711
Gene: ENSMUSG00000075316
AA Change: V851A

Domain	Start	End	E-Value	Type
low complexity region	29	49	N/A	INTRINSIC
Pfam:Ion_trans	154	401	3.7e-78	PFAM
coiled coil region	402	449	N/A	INTRINSIC
Pfam:DUF3451	463	683	1.3e-62	PFAM
Pfam:Ion_trans	766	955	9.9e-48	PFAM
Pfam:Na_trans_assoc	970	1189	2.9e-72	PFAM
low complexity region	1201	1212	N/A	INTRINSIC
Pfam:Ion_trans	1215	1443	2.8e-55	PFAM
PDB:1BYY\|A	1445	1497	7e-29	PDB
Pfam:Ion_trans	1536	1746	3.4e-52	PFAM
Pfam:PKD_channel	1597	1753	1.1e-7	PFAM
IQ	1875	1897	1.03e-3	SMART
low complexity region	1954	1970	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia, channelopathy-associated insensitivity to pain, and paroxysmal extreme pain disorder. [provided by RefSeq, Aug 2009]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit prenatal/neonatal lethality. Mice homozygous for a knock-in allele exhibit increased susceptibility to electrically induced seizures. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad10	A	T	5: 121,656,012 (GRCm38)		probably null	Het
Ahnak	T	C	19: 9,004,822 (GRCm38)	S1157P	probably damaging	Het
Akap12	T	G	10: 4,353,967 (GRCm38)	V364G	probably benign	Het
Ankrd17	A	G	5: 90,299,996 (GRCm38)	S229P	probably benign	Het
Apof	A	T	10: 128,268,767 (GRCm38)	Y5F	probably benign	Het
Arhgap24	T	A	5: 102,841,308 (GRCm38)	M94K	probably benign	Het
Ash2l	G	T	8: 25,822,770 (GRCm38)	D479E	probably damaging	Het
Atp8a2	A	T	14: 60,008,375 (GRCm38)	C623S	probably benign	Het
Bmper	T	A	9: 23,483,942 (GRCm38)	N656K	probably benign	Het
Braf	T	C	6: 39,627,838 (GRCm38)	I619V	possibly damaging	Het
Brwd1	T	C	16: 96,056,173 (GRCm38)	R396G	probably damaging	Het
Car7	A	T	8: 104,549,584 (GRCm38)	T234S	probably benign	Het
Catsper4	A	T	4: 134,218,241 (GRCm38)	V169E	probably damaging	Het
Cemip	T	G	7: 83,998,576 (GRCm38)	I99L	probably damaging	Het
Chrna7	C	A	7: 63,104,990 (GRCm38)	V290L	probably damaging	Het
Clip1	A	T	5: 123,617,384 (GRCm38)	D944E	probably benign	Het
Cyp2c68	T	C	19: 39,689,137 (GRCm38)	T470A	probably benign	Het
Dmbx1	G	T	4: 115,923,711 (GRCm38)	A31D	probably damaging	Het
Dnah7b	T	C	1: 46,175,419 (GRCm38)	F1115L	probably damaging	Het
Dock10	T	C	1: 80,515,566 (GRCm38)	I1897V	probably benign	Het
Efcab3	A	G	11: 105,109,286 (GRCm38)	I234V	probably benign	Het
Eid2b	T	G	7: 28,277,818 (GRCm38)	L13R	probably damaging	Het
Enam	A	G	5: 88,501,820 (GRCm38)	Y396C	probably damaging	Het
Fam161a	A	T	11: 23,021,006 (GRCm38)	T395S	probably damaging	Het
Fat1	A	G	8: 45,023,160 (GRCm38)	R1748G	probably benign	Het
Fdxacb1	C	A	9: 50,770,151 (GRCm38)	T124K	possibly damaging	Het
Fer1l6	A	T	15: 58,641,945 (GRCm38)	D1563V	possibly damaging	Het
Gm20834	T	C	Y: 10,323,226 (GRCm38)	H70R	probably damaging	Het
Gucy2d	T	A	7: 98,449,757 (GRCm38)	M261K	probably damaging	Het
Hcar2	T	A	5: 123,864,798 (GRCm38)	Q214L	probably benign	Het
Hdac11	C	T	6: 91,159,232 (GRCm38)	H75Y	probably damaging	Het
Hoxa2	C	A	6: 52,164,299 (GRCm38)	A116S	probably benign	Het
Ints2	A	G	11: 86,215,618 (GRCm38)	C989R	probably damaging	Het
Jpt1	T	C	11: 115,503,124 (GRCm38)	E36G	probably damaging	Het
Kdm5d	G	A	Y: 914,044 (GRCm38)	R327Q	possibly damaging	Het
Klhl1	T	C	14: 96,346,868 (GRCm38)	M309V	probably benign	Het
Larp4	A	G	15: 99,991,778 (GRCm38)	K193E	probably benign	Het
Lmo3	C	T	6: 138,416,502 (GRCm38)	C40Y	probably damaging	Het
Mcm4	A	G	16: 15,630,442 (GRCm38)	V417A	probably benign	Het
Med8	A	G	4: 118,410,979 (GRCm38)	E34G	probably damaging	Het
Mex3b	G	T	7: 82,867,906 (GRCm38)	A86S	possibly damaging	Het
Muc6	T	C	7: 141,639,823 (GRCm38)	T1646A	unknown	Het
Myo15b	A	T	11: 115,858,744 (GRCm38)	E113D		Het
Myo7a	T	A	7: 98,063,674 (GRCm38)	H1698L	probably benign	Het
Myocos	T	A	1: 162,657,109 (GRCm38)	M44L	unknown	Het
Nalcn	A	G	14: 123,314,087 (GRCm38)	V1036A	probably damaging	Het
Nfrkb	C	T	9: 31,414,032 (GRCm38)	Q924*	probably null	Het
Nol6	A	G	4: 41,117,424 (GRCm38)	L923P	possibly damaging	Het
Nrxn3	T	C	12: 89,510,462 (GRCm38)	Y472H	probably damaging	Het
Nudcd1	A	G	15: 44,405,857 (GRCm38)	Y136H	possibly damaging	Het
Obox2	T	C	7: 15,397,316 (GRCm38)	C116R	probably damaging	Het
Olfr1309	T	C	2: 111,983,779 (GRCm38)	I98M	probably damaging	Het
Olfr1333	A	G	4: 118,830,320 (GRCm38)	V39A	probably damaging	Het
Olfr715b	T	A	7: 107,106,775 (GRCm38)	I29F	probably benign	Het
Olfr743	T	A	14: 50,534,015 (GRCm38)	V201D	probably benign	Het
Onecut3	A	T	10: 80,495,476 (GRCm38)	S157C	unknown	Het
Oscp1	C	T	4: 126,073,933 (GRCm38)	R91*	probably null	Het
Pclo	A	T	5: 14,712,592 (GRCm38)	D408V		Het
Pde8a	T	C	7: 81,282,833 (GRCm38)	V81A	probably benign	Het
Pde8b	T	A	13: 95,027,743 (GRCm38)	D653V	probably damaging	Het
Peg10	T	TCCG	6: 4,756,451 (GRCm38)		probably benign	Het
Pik3cg	T	C	12: 32,195,648 (GRCm38)	Y867C	probably damaging	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 109,624,195 (GRCm38)		probably benign	Het
Psd4	A	T	2: 24,404,756 (GRCm38)	D776V	possibly damaging	Het
Ptprb	A	G	10: 116,283,429 (GRCm38)	E156G	probably benign	Het
Rad54b	A	G	4: 11,610,372 (GRCm38)	Y663C	probably damaging	Het
Ranbp3l	T	A	15: 9,002,132 (GRCm38)	V78D	possibly damaging	Het
Rbm12	T	C	2: 156,098,218 (GRCm38)	I45V	unknown	Het
Rsg1	A	G	4: 141,219,842 (GRCm38)	D178G	probably damaging	Het
Sdcbp	T	C	4: 6,393,089 (GRCm38)	I242T	possibly damaging	Het
Slc25a24	A	G	3: 109,159,435 (GRCm38)	T302A	probably damaging	Het
Slc45a3	G	T	1: 131,976,811 (GRCm38)		probably benign	Het
Srpk1	T	C	17: 28,594,218 (GRCm38)	I517M	probably benign	Het
Tmx4	A	G	2: 134,639,661 (GRCm38)	M114T	probably benign	Het
Trhr	T	A	15: 44,229,231 (GRCm38)	V288D	probably damaging	Het
Tspoap1	A	T	11: 87,761,525 (GRCm38)	I42F	probably benign	Het
Ttn	A	T	2: 76,951,512 (GRCm38)	D1063E	unknown	Het
Tusc3	A	G	8: 39,071,481 (GRCm38)	I225V	probably benign	Het
Ugt2b38	A	G	5: 87,424,114 (GRCm38)	S20P	probably damaging	Het
Upf2	A	G	2: 6,027,408 (GRCm38)	T922A	unknown	Het
Usp38	C	T	8: 81,014,561 (GRCm38)		probably benign	Het
Vmn1r216	A	G	13: 23,099,568 (GRCm38)	I140M	probably damaging	Het
Vmn2r115	T	A	17: 23,346,397 (GRCm38)	N419K	possibly damaging	Het
Vmn2r55	C	T	7: 12,670,828 (GRCm38)	G216D	probably benign	Het
Vwa8	A	C	14: 78,982,234 (GRCm38)		probably null	Het
Wnt7b	C	A	15: 85,537,414 (GRCm38)	R349L	possibly damaging	Het
Xbp1	T	C	11: 5,521,098 (GRCm38)	V35A	probably benign	Het
Zan	A	T	5: 137,446,795 (GRCm38)	M1734K	unknown	Het
Zfhx4	A	G	3: 5,412,177 (GRCm38)	E3284G	probably damaging	Het
Zfp458	A	G	13: 67,256,914 (GRCm38)	L487S	possibly damaging	Het
Zfp687	C	T	3: 95,007,530 (GRCm38)	R1220H	probably damaging	Het
Zp1	A	G	19: 10,917,916 (GRCm38)	L324P	possibly damaging	Het

Other mutations in Scn9a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00514:Scn9a	APN	2	66,563,601 (GRCm38)	missense	probably damaging	1.00
IGL00570:Scn9a	APN	2	66,484,142 (GRCm38)	missense	probably damaging	1.00
IGL00809:Scn9a	APN	2	66,483,935 (GRCm38)	missense	probably damaging	1.00
IGL00977:Scn9a	APN	2	66,484,301 (GRCm38)	missense	probably damaging	0.99
IGL01120:Scn9a	APN	2	66,526,972 (GRCm38)	missense	probably benign	0.00
IGL01134:Scn9a	APN	2	66,504,968 (GRCm38)	missense	probably damaging	1.00
IGL01300:Scn9a	APN	2	66,488,053 (GRCm38)	nonsense	probably null
IGL01452:Scn9a	APN	2	66,527,072 (GRCm38)	missense	probably damaging	1.00
IGL01531:Scn9a	APN	2	66,537,378 (GRCm38)	missense	probably benign	0.11
IGL01572:Scn9a	APN	2	66,493,886 (GRCm38)	missense	probably benign	0.00
IGL01645:Scn9a	APN	2	66,487,642 (GRCm38)	missense	possibly damaging	0.62
IGL01823:Scn9a	APN	2	66,484,042 (GRCm38)	missense	probably damaging	1.00
IGL01965:Scn9a	APN	2	66,484,433 (GRCm38)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,494,826 (GRCm38)	missense	probably damaging	1.00
IGL02127:Scn9a	APN	2	66,547,135 (GRCm38)	missense	probably damaging	1.00
IGL02166:Scn9a	APN	2	66,493,103 (GRCm38)	missense	possibly damaging	0.95
IGL02183:Scn9a	APN	2	66,484,611 (GRCm38)	splice site	probably benign
IGL02640:Scn9a	APN	2	66,536,096 (GRCm38)	critical splice donor site	probably null
IGL02685:Scn9a	APN	2	66,537,293 (GRCm38)	missense	probably damaging	1.00
IGL02798:Scn9a	APN	2	66,540,559 (GRCm38)	missense	possibly damaging	0.52
IGL02832:Scn9a	APN	2	66,568,029 (GRCm38)	missense	probably damaging	1.00
IGL03008:Scn9a	APN	2	66,562,511 (GRCm38)	missense	probably damaging	1.00
IGL03270:Scn9a	APN	2	66,484,014 (GRCm38)	missense	probably damaging	1.00
IGL03408:Scn9a	APN	2	66,526,747 (GRCm38)	missense	probably benign	0.00
BB007:Scn9a	UTSW	2	66,504,849 (GRCm38)	missense	probably damaging	0.99
BB017:Scn9a	UTSW	2	66,504,849 (GRCm38)	missense	probably damaging	0.99
R0039:Scn9a	UTSW	2	66,562,444 (GRCm38)	missense	probably damaging	0.98
R0173:Scn9a	UTSW	2	66,533,093 (GRCm38)	missense	probably damaging	1.00
R0323:Scn9a	UTSW	2	66,568,131 (GRCm38)	missense	probably damaging	1.00
R0344:Scn9a	UTSW	2	66,505,010 (GRCm38)	missense	probably damaging	0.99
R0421:Scn9a	UTSW	2	66,543,277 (GRCm38)	missense	probably benign
R0465:Scn9a	UTSW	2	66,526,996 (GRCm38)	missense	probably damaging	1.00
R0514:Scn9a	UTSW	2	66,483,678 (GRCm38)	missense	probably damaging	1.00
R0599:Scn9a	UTSW	2	66,526,799 (GRCm38)	missense	probably damaging	0.96
R0627:Scn9a	UTSW	2	66,537,377 (GRCm38)	missense	probably benign	0.00
R0644:Scn9a	UTSW	2	66,533,061 (GRCm38)	critical splice donor site	probably null
R0653:Scn9a	UTSW	2	66,533,377 (GRCm38)	missense	probably damaging	1.00
R0685:Scn9a	UTSW	2	66,483,499 (GRCm38)	missense	probably benign	0.02
R0718:Scn9a	UTSW	2	66,547,112 (GRCm38)	missense	probably damaging	1.00
R0827:Scn9a	UTSW	2	66,536,124 (GRCm38)	nonsense	probably null
R0890:Scn9a	UTSW	2	66,483,735 (GRCm38)	missense	probably damaging	1.00
R1139:Scn9a	UTSW	2	66,504,997 (GRCm38)	missense	probably benign	0.02
R1385:Scn9a	UTSW	2	66,563,542 (GRCm38)	missense	probably damaging	1.00
R1398:Scn9a	UTSW	2	66,484,586 (GRCm38)	missense	probably benign	0.11
R1496:Scn9a	UTSW	2	66,526,888 (GRCm38)	missense	probably benign
R1511:Scn9a	UTSW	2	66,526,813 (GRCm38)	missense	probably benign	0.01
R1517:Scn9a	UTSW	2	66,505,027 (GRCm38)	splice site	probably benign
R1564:Scn9a	UTSW	2	66,484,304 (GRCm38)	missense	probably damaging	1.00
R1634:Scn9a	UTSW	2	66,488,017 (GRCm38)	missense	probably damaging	1.00
R1662:Scn9a	UTSW	2	66,483,459 (GRCm38)	missense	probably benign	0.00
R1695:Scn9a	UTSW	2	66,504,876 (GRCm38)	nonsense	probably null
R1709:Scn9a	UTSW	2	66,483,506 (GRCm38)	missense	probably damaging	1.00
R1741:Scn9a	UTSW	2	66,487,594 (GRCm38)	missense	probably damaging	0.99
R1755:Scn9a	UTSW	2	66,501,716 (GRCm38)	missense	probably benign	0.38
R1914:Scn9a	UTSW	2	66,566,250 (GRCm38)	missense	probably damaging	1.00
R1962:Scn9a	UTSW	2	66,484,311 (GRCm38)	missense	probably damaging	1.00
R1970:Scn9a	UTSW	2	66,515,380 (GRCm38)	missense	probably damaging	0.97
R2017:Scn9a	UTSW	2	66,515,321 (GRCm38)	missense	probably damaging	0.99
R2092:Scn9a	UTSW	2	66,533,376 (GRCm38)	missense	probably damaging	0.99
R2105:Scn9a	UTSW	2	66,568,183 (GRCm38)	missense	probably benign	0.25
R2114:Scn9a	UTSW	2	66,484,052 (GRCm38)	missense	probably damaging	1.00
R2115:Scn9a	UTSW	2	66,484,052 (GRCm38)	missense	probably damaging	1.00
R2128:Scn9a	UTSW	2	66,526,654 (GRCm38)	missense	probably damaging	1.00
R2157:Scn9a	UTSW	2	66,536,325 (GRCm38)	missense	probably damaging	1.00
R2162:Scn9a	UTSW	2	66,534,229 (GRCm38)	missense	probably damaging	0.98
R2350:Scn9a	UTSW	2	66,504,968 (GRCm38)	missense	probably damaging	1.00
R3694:Scn9a	UTSW	2	66,562,405 (GRCm38)	missense	probably benign
R3771:Scn9a	UTSW	2	66,483,648 (GRCm38)	missense	probably benign	0.26
R3772:Scn9a	UTSW	2	66,483,648 (GRCm38)	missense	probably benign	0.26
R3773:Scn9a	UTSW	2	66,483,648 (GRCm38)	missense	probably benign	0.26
R3922:Scn9a	UTSW	2	66,526,873 (GRCm38)	missense	possibly damaging	0.88
R3926:Scn9a	UTSW	2	66,526,873 (GRCm38)	missense	possibly damaging	0.88
R4258:Scn9a	UTSW	2	66,565,054 (GRCm38)	intron	probably benign
R4385:Scn9a	UTSW	2	66,484,556 (GRCm38)	missense	probably damaging	1.00
R4415:Scn9a	UTSW	2	66,526,693 (GRCm38)	missense	probably damaging	1.00
R4570:Scn9a	UTSW	2	66,483,558 (GRCm38)	missense	possibly damaging	0.85
R4682:Scn9a	UTSW	2	66,547,018 (GRCm38)	missense	probably benign
R4783:Scn9a	UTSW	2	66,540,623 (GRCm38)	missense	probably benign	0.01
R4822:Scn9a	UTSW	2	66,483,749 (GRCm38)	missense	possibly damaging	0.55
R4829:Scn9a	UTSW	2	66,551,713 (GRCm38)	missense	probably benign
R4908:Scn9a	UTSW	2	66,526,743 (GRCm38)	missense	probably benign	0.03
R4983:Scn9a	UTSW	2	66,566,270 (GRCm38)	missense	probably benign	0.02
R5047:Scn9a	UTSW	2	66,562,480 (GRCm38)	missense	probably damaging	1.00
R5100:Scn9a	UTSW	2	66,534,119 (GRCm38)	missense	probably damaging	1.00
R5140:Scn9a	UTSW	2	66,565,167 (GRCm38)	missense	possibly damaging	0.81
R5398:Scn9a	UTSW	2	66,488,043 (GRCm38)	missense	probably damaging	1.00
R5557:Scn9a	UTSW	2	66,547,103 (GRCm38)	missense	probably damaging	0.99
R5582:Scn9a	UTSW	2	66,565,029 (GRCm38)	intron	probably benign
R6108:Scn9a	UTSW	2	66,484,049 (GRCm38)	missense	probably damaging	1.00
R6115:Scn9a	UTSW	2	66,563,629 (GRCm38)	missense	possibly damaging	0.70
R6143:Scn9a	UTSW	2	66,487,524 (GRCm38)	missense	probably benign	0.00
R6261:Scn9a	UTSW	2	66,483,896 (GRCm38)	missense	probably damaging	1.00
R6335:Scn9a	UTSW	2	66,568,264 (GRCm38)	start codon destroyed	possibly damaging	0.91
R6429:Scn9a	UTSW	2	66,526,963 (GRCm38)	missense	possibly damaging	0.95
R6632:Scn9a	UTSW	2	66,483,502 (GRCm38)	missense	probably benign	0.23
R6681:Scn9a	UTSW	2	66,563,342 (GRCm38)	missense	possibly damaging	0.90
R6830:Scn9a	UTSW	2	66,568,029 (GRCm38)	missense	probably damaging	1.00
R7102:Scn9a	UTSW	2	66,549,015 (GRCm38)	missense	probably damaging	1.00
R7186:Scn9a	UTSW	2	66,534,223 (GRCm38)	missense	probably damaging	1.00
R7243:Scn9a	UTSW	2	66,540,530 (GRCm38)	missense	probably damaging	1.00
R7311:Scn9a	UTSW	2	66,484,404 (GRCm38)	missense	possibly damaging	0.54
R7328:Scn9a	UTSW	2	66,484,587 (GRCm38)	missense	probably benign
R7386:Scn9a	UTSW	2	66,540,550 (GRCm38)	missense	probably damaging	1.00
R7438:Scn9a	UTSW	2	66,547,187 (GRCm38)	missense	possibly damaging	0.81
R7485:Scn9a	UTSW	2	66,534,217 (GRCm38)	missense	probably damaging	1.00
R7526:Scn9a	UTSW	2	66,483,646 (GRCm38)	missense	probably benign
R7617:Scn9a	UTSW	2	66,540,549 (GRCm38)	missense	possibly damaging	0.55
R7642:Scn9a	UTSW	2	66,536,236 (GRCm38)	missense	probably benign	0.02
R7653:Scn9a	UTSW	2	66,527,080 (GRCm38)	missense	probably damaging	1.00
R7747:Scn9a	UTSW	2	66,484,298 (GRCm38)	missense	probably damaging	1.00
R7823:Scn9a	UTSW	2	66,483,791 (GRCm38)	missense	probably damaging	1.00
R7864:Scn9a	UTSW	2	66,484,560 (GRCm38)	missense	possibly damaging	0.73
R7890:Scn9a	UTSW	2	66,543,112 (GRCm38)	missense	probably benign	0.00
R7930:Scn9a	UTSW	2	66,504,849 (GRCm38)	missense	probably damaging	0.99
R7975:Scn9a	UTSW	2	66,484,253 (GRCm38)	missense	probably damaging	1.00
R8057:Scn9a	UTSW	2	66,515,430 (GRCm38)	missense	probably benign	0.06
R8145:Scn9a	UTSW	2	66,487,410 (GRCm38)	missense	probably damaging	1.00
R8163:Scn9a	UTSW	2	66,484,401 (GRCm38)	missense	probably damaging	1.00
R8165:Scn9a	UTSW	2	66,540,530 (GRCm38)	missense	probably damaging	1.00
R8342:Scn9a	UTSW	2	66,536,282 (GRCm38)	missense	probably benign
R8345:Scn9a	UTSW	2	66,494,622 (GRCm38)	missense	probably damaging	0.96
R8464:Scn9a	UTSW	2	66,566,281 (GRCm38)	missense	probably damaging	0.99
R8467:Scn9a	UTSW	2	66,501,671 (GRCm38)	missense	probably damaging	1.00
R8698:Scn9a	UTSW	2	66,536,284 (GRCm38)	missense	probably benign	0.00
R8810:Scn9a	UTSW	2	66,501,666 (GRCm38)	missense	probably damaging	1.00
R8822:Scn9a	UTSW	2	66,540,635 (GRCm38)	missense	probably damaging	0.99
R8829:Scn9a	UTSW	2	66,483,617 (GRCm38)	missense	probably benign
R9009:Scn9a	UTSW	2	66,508,583 (GRCm38)	missense	probably damaging	1.00
R9038:Scn9a	UTSW	2	66,494,803 (GRCm38)	missense	probably damaging	1.00
R9126:Scn9a	UTSW	2	66,484,400 (GRCm38)	missense	probably damaging	1.00
R9205:Scn9a	UTSW	2	66,533,313 (GRCm38)	missense	probably damaging	1.00
R9300:Scn9a	UTSW	2	66,504,892 (GRCm38)	missense	probably benign	0.39
R9373:Scn9a	UTSW	2	66,483,917 (GRCm38)	missense	probably benign	0.00
R9404:Scn9a	UTSW	2	66,526,696 (GRCm38)	missense	probably benign	0.02
R9443:Scn9a	UTSW	2	66,565,209 (GRCm38)	missense	probably damaging	1.00
R9590:Scn9a	UTSW	2	66,483,984 (GRCm38)	missense	probably benign	0.05
R9612:Scn9a	UTSW	2	66,533,364 (GRCm38)	missense	probably damaging	1.00
R9617:Scn9a	UTSW	2	66,562,465 (GRCm38)	missense	probably damaging	1.00
R9717:Scn9a	UTSW	2	66,526,658 (GRCm38)	missense	probably benign
X0003:Scn9a	UTSW	2	66,508,647 (GRCm38)	missense	probably benign	0.02
X0062:Scn9a	UTSW	2	66,568,077 (GRCm38)	missense	probably damaging	1.00
Z1176:Scn9a	UTSW	2	66,540,592 (GRCm38)	missense	probably benign	0.00
Z1177:Scn9a	UTSW	2	66,494,685 (GRCm38)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- GCAGTCCCACATGGTCTCTATC -3'
(R):5'- CTCTTTAGCTACACAAAAGTAACGG -3'

Sequencing Primer
(F):5'- GTCTCTATCCACTCCCCACACAG -3'
(R):5'- TAACGGAATGTGAGGTATACTGC -3'

Posted On

2019-10-07