Mutagenetix > Incidental Mutation

Incidental Mutation 'R7484:Or10ag60'

579977

Institutional Source

Beutler Lab

Gene Symbol

Or10ag60

Ensembl Gene

ENSMUSG00000059205

Gene Name

olfactory receptor family 10 subfamily AG member 60

Synonyms

GA_x6K02T2Q125-49112575-49113519, Olfr1130, MOR264-4

MMRRC Submission

045558-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.176) question?

Stock #

R7484 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

87436556-87438678 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 87438281 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Serine at position 183 (F183S)

Ref Sequence

ENSEMBL: ENSMUSP00000150609 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000213103] [ENSMUST00000216580]

AlphaFold

A0A1L1SQT2

Predicted Effect

probably damaging
Transcript: ENSMUST00000213103
AA Change: F183S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Predicted Effect

probably damaging
Transcript: ENSMUST00000216580
AA Change: F183S

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

Meta Mutation Damage Score

0.4272

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

98% (65/66)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl4fm5	A	G	4: 144,504,521 (GRCm39)	L210P	probably damaging	Het
Acacb	G	A	5: 114,356,923 (GRCm39)	V1285I	probably damaging	Het
Acly	A	T	11: 100,386,789 (GRCm39)	I591N	probably damaging	Het
Acr	T	A	15: 89,457,427 (GRCm39)	V225E	probably damaging	Het
Actr8	A	G	14: 29,714,925 (GRCm39)	D580G	probably damaging	Het
Adamts12	C	A	15: 11,345,734 (GRCm39)	Q1592K	probably benign	Het
Aire	T	C	10: 77,878,404 (GRCm39)	E138G	probably damaging	Het
Apob	C	T	12: 8,056,884 (GRCm39)	Q1789*	probably null	Het
Cbx5	A	C	15: 103,114,256 (GRCm39)		probably null	Het
Cd300lb	A	T	11: 114,819,345 (GRCm39)	W95R	probably damaging	Het
Cdc16	A	G	8: 13,827,605 (GRCm39)	T504A	probably benign	Het
Cenpf	A	G	1: 189,389,018 (GRCm39)	S1605P	probably damaging	Het
Ckap2l	A	G	2: 129,114,455 (GRCm39)	V596A	possibly damaging	Het
Cntn1	T	C	15: 92,151,922 (GRCm39)	W454R	probably benign	Het
Crat	C	T	2: 30,294,577 (GRCm39)	R497Q	probably benign	Het
Csf2rb	A	G	15: 78,223,099 (GRCm39)	T104A	possibly damaging	Het
Cyp4a12b	A	G	4: 115,289,760 (GRCm39)	D209G	possibly damaging	Het
Ddx23	T	C	15: 98,546,570 (GRCm39)	E533G	probably damaging	Het
Dscaml1	A	G	9: 45,660,744 (GRCm39)		probably null	Het
Eif3l	T	G	15: 78,968,336 (GRCm39)	C202G	probably benign	Het
Ephx4	T	G	5: 107,577,612 (GRCm39)	M312R	probably damaging	Het
Erich6	A	T	3: 58,534,112 (GRCm39)		probably null	Het
Far2	G	A	6: 148,075,411 (GRCm39)	D424N	probably damaging	Het
Fat1	C	A	8: 45,489,221 (GRCm39)	N3497K	probably damaging	Het
Fstl3	G	A	10: 79,615,865 (GRCm39)	C117Y	probably damaging	Het
Fyb2	A	T	4: 104,870,499 (GRCm39)	H700L	probably benign	Het
Gm8356	T	A	14: 17,691,282 (GRCm39)	M128L	probably benign	Het
Golim4	G	T	3: 75,805,442 (GRCm39)		probably null	Het
Grm1	T	G	10: 10,622,403 (GRCm39)	D440A	probably benign	Het
Gtf2a1	A	G	12: 91,529,747 (GRCm39)	V322A	probably benign	Het
Hid1	T	C	11: 115,243,407 (GRCm39)		probably null	Het
Igfals	G	A	17: 25,098,962 (GRCm39)	V18M	possibly damaging	Het
Klk1b24	G	T	7: 43,839,688 (GRCm39)		probably null	Het
Lmbr1	T	C	5: 29,551,850 (GRCm39)		probably benign	Het
Lrrc40	T	C	3: 157,746,194 (GRCm39)	S90P	probably benign	Het
Mapk9	T	C	11: 49,763,663 (GRCm39)	Y185H	probably damaging	Het
Marveld2	C	T	13: 100,748,068 (GRCm39)	G337D	probably damaging	Het
Mga	G	T	2: 119,776,710 (GRCm39)	R1539L	probably damaging	Het
Mllt6	T	A	11: 97,563,442 (GRCm39)	S342T	probably benign	Het
Ms4a6c	T	C	19: 11,449,893 (GRCm39)		probably null	Het
Muc16	T	A	9: 18,558,064 (GRCm39)	H2743L	unknown	Het
Myl10	G	C	5: 136,726,825 (GRCm39)	V70L	probably benign	Het
Or2l13	A	G	16: 19,305,753 (GRCm39)	H55R	possibly damaging	Het
Plcd3	T	G	11: 102,962,545 (GRCm39)	K635N	probably damaging	Het
Prkch	T	A	12: 73,632,301 (GRCm39)		probably null	Het
Rb1cc1	T	C	1: 6,344,441 (GRCm39)	V1570A	probably damaging	Het
Rp1	T	C	1: 4,415,704 (GRCm39)	N1803D	probably benign	Het
Rufy3	T	A	5: 88,746,331 (GRCm39)	V72E	probably benign	Het
Secisbp2l	G	A	2: 125,613,452 (GRCm39)	Q181*	probably null	Het
Sgcb	A	G	5: 73,797,188 (GRCm39)	F191L	possibly damaging	Het
Sgsh	T	A	11: 119,237,183 (GRCm39)	D477V	probably damaging	Het
Shoc1	A	T	4: 59,062,286 (GRCm39)	V924E	probably damaging	Het
Slc22a28	C	A	19: 8,048,492 (GRCm39)	S385I	probably benign	Het
Slc26a3	T	C	12: 31,497,787 (GRCm39)	V47A	probably benign	Het
Slco2a1	A	T	9: 102,945,185 (GRCm39)	I187F	probably damaging	Het
Sltm	T	C	9: 70,481,179 (GRCm39)	S344P	unknown	Het
Sort1	A	G	3: 108,246,141 (GRCm39)	T373A	probably damaging	Het
Stab1	A	G	14: 30,882,274 (GRCm39)	F474L	probably benign	Het
Tbc1d5	G	T	17: 51,224,573 (GRCm39)	A326E	possibly damaging	Het
Tcstv4	A	T	13: 120,770,017 (GRCm39)	K112N	probably damaging	Het
Vwa8	A	C	14: 79,219,674 (GRCm39)		probably null	Het
Wdr70	G	A	15: 7,951,562 (GRCm39)	T425I	probably benign	Het
Zfp30	T	C	7: 29,492,231 (GRCm39)	Y243H	probably benign	Het
Zfp974	A	T	7: 27,611,559 (GRCm39)	N55K	possibly damaging	Het
Zftraf1	A	G	15: 76,530,435 (GRCm39)	L295P	probably damaging	Het

Other mutations in Or10ag60

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01151:Or10ag60	APN	2	87,438,323 (GRCm39)	missense	probably damaging	1.00
IGL01310:Or10ag60	APN	2	87,437,852 (GRCm39)	missense	possibly damaging	0.68
IGL02558:Or10ag60	APN	2	87,437,903 (GRCm39)	missense	probably benign	0.00
R0323:Or10ag60	UTSW	2	87,437,841 (GRCm39)	missense	probably benign
R0590:Or10ag60	UTSW	2	87,438,338 (GRCm39)	missense	probably damaging	0.98
R0718:Or10ag60	UTSW	2	87,438,271 (GRCm39)	missense	probably benign	0.08
R2445:Or10ag60	UTSW	2	87,438,302 (GRCm39)	missense	probably damaging	0.99
R3408:Or10ag60	UTSW	2	87,438,220 (GRCm39)	missense	probably benign	0.03
R4280:Or10ag60	UTSW	2	87,438,595 (GRCm39)	missense	possibly damaging	0.90
R4491:Or10ag60	UTSW	2	87,437,736 (GRCm39)	start codon destroyed	probably null	0.84
R4928:Or10ag60	UTSW	2	87,438,487 (GRCm39)	missense	probably benign	0.06
R5033:Or10ag60	UTSW	2	87,438,055 (GRCm39)	missense	probably damaging	1.00
R5061:Or10ag60	UTSW	2	87,438,176 (GRCm39)	missense	probably benign	0.00
R5109:Or10ag60	UTSW	2	87,438,319 (GRCm39)	missense	possibly damaging	0.78
R5109:Or10ag60	UTSW	2	87,437,755 (GRCm39)	missense	possibly damaging	0.64
R5772:Or10ag60	UTSW	2	87,438,517 (GRCm39)	missense	probably benign	0.12
R6004:Or10ag60	UTSW	2	87,438,253 (GRCm39)	missense	probably damaging	1.00
R6005:Or10ag60	UTSW	2	87,438,424 (GRCm39)	missense	probably damaging	0.97
R6411:Or10ag60	UTSW	2	87,438,317 (GRCm39)	missense	probably damaging	1.00
R6964:Or10ag60	UTSW	2	87,437,957 (GRCm39)	missense	probably damaging	1.00
R7085:Or10ag60	UTSW	2	87,437,750 (GRCm39)	missense	probably benign	0.00
R8429:Or10ag60	UTSW	2	87,437,868 (GRCm39)	missense	probably benign	0.06
R8471:Or10ag60	UTSW	2	87,437,989 (GRCm39)	missense	probably damaging	0.99
R8485:Or10ag60	UTSW	2	87,438,601 (GRCm39)	missense	probably benign	0.00
R8890:Or10ag60	UTSW	2	87,438,412 (GRCm39)	missense	probably damaging	1.00
R8935:Or10ag60	UTSW	2	87,438,421 (GRCm39)	missense	possibly damaging	0.72
X0060:Or10ag60	UTSW	2	87,438,386 (GRCm39)	missense	probably benign	0.11
Z1176:Or10ag60	UTSW	2	87,438,098 (GRCm39)	missense	probably benign	0.34

Predicted Primers

PCR Primer
(F):5'- CAGCCACTGAGTGTTTCCTG -3'
(R):5'- ACATGTTGAGAAGGCTTTTGC -3'

Sequencing Primer
(F):5'- GCTGTGATGTCTTATGACCGC -3'
(R):5'- CCCGTCCTGTGGCTGTTG -3'

Posted On

2019-10-07