Mutagenetix > Incidental Mutation

Incidental Mutation 'R7484:Slco2a1'

580001

Institutional Source

Beutler Lab

Gene Symbol

Slco2a1

Ensembl Gene

ENSMUSG00000032548

Gene Name

solute carrier organic anion transporter family, member 2a1

Synonyms

Pgt, mPgt, Slc21a2, 2310021C19Rik

MMRRC Submission

045558-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7484 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

102885686-102973201 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 102945185 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 187 (I187F)

Ref Sequence

ENSEMBL: ENSMUSP00000035148 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035148] [ENSMUST00000188664]

AlphaFold

Q9EPT5

Predicted Effect

probably damaging
Transcript: ENSMUST00000035148
AA Change: I187F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000035148
Gene: ENSMUSG00000032548
AA Change: I187F

Domain	Start	End	E-Value	Type
Pfam:MFS_1	39	428	3.5e-22	PFAM
KAZAL	446	493	2.78e-2	SMART
transmembrane domain	605	627	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000188664
AA Change: I187F

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000140533
Gene: ENSMUSG00000032548
AA Change: I187F

Domain	Start	End	E-Value	Type
Pfam:OATP	31	381	4.8e-135	PFAM
Pfam:MFS_1	39	413	1.8e-19	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

98% (65/66)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a prostaglandin transporter that is a member of the 12-membrane-spanning superfamily of transporters. The encoded protein may be involved in mediating the uptake and clearance of prostaglandins in numerous tissues. [provided by RefSeq, Dec 2011]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit preinatel or early psotnatal lethality due to a patent ductus arteriosus and abnormal protaglandin metabolism. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl4fm5	A	G	4: 144,504,521 (GRCm39)	L210P	probably damaging	Het
Acacb	G	A	5: 114,356,923 (GRCm39)	V1285I	probably damaging	Het
Acly	A	T	11: 100,386,789 (GRCm39)	I591N	probably damaging	Het
Acr	T	A	15: 89,457,427 (GRCm39)	V225E	probably damaging	Het
Actr8	A	G	14: 29,714,925 (GRCm39)	D580G	probably damaging	Het
Adamts12	C	A	15: 11,345,734 (GRCm39)	Q1592K	probably benign	Het
Aire	T	C	10: 77,878,404 (GRCm39)	E138G	probably damaging	Het
Apob	C	T	12: 8,056,884 (GRCm39)	Q1789*	probably null	Het
Cbx5	A	C	15: 103,114,256 (GRCm39)		probably null	Het
Cd300lb	A	T	11: 114,819,345 (GRCm39)	W95R	probably damaging	Het
Cdc16	A	G	8: 13,827,605 (GRCm39)	T504A	probably benign	Het
Cenpf	A	G	1: 189,389,018 (GRCm39)	S1605P	probably damaging	Het
Ckap2l	A	G	2: 129,114,455 (GRCm39)	V596A	possibly damaging	Het
Cntn1	T	C	15: 92,151,922 (GRCm39)	W454R	probably benign	Het
Crat	C	T	2: 30,294,577 (GRCm39)	R497Q	probably benign	Het
Csf2rb	A	G	15: 78,223,099 (GRCm39)	T104A	possibly damaging	Het
Cyp4a12b	A	G	4: 115,289,760 (GRCm39)	D209G	possibly damaging	Het
Ddx23	T	C	15: 98,546,570 (GRCm39)	E533G	probably damaging	Het
Dscaml1	A	G	9: 45,660,744 (GRCm39)		probably null	Het
Eif3l	T	G	15: 78,968,336 (GRCm39)	C202G	probably benign	Het
Ephx4	T	G	5: 107,577,612 (GRCm39)	M312R	probably damaging	Het
Erich6	A	T	3: 58,534,112 (GRCm39)		probably null	Het
Far2	G	A	6: 148,075,411 (GRCm39)	D424N	probably damaging	Het
Fat1	C	A	8: 45,489,221 (GRCm39)	N3497K	probably damaging	Het
Fstl3	G	A	10: 79,615,865 (GRCm39)	C117Y	probably damaging	Het
Fyb2	A	T	4: 104,870,499 (GRCm39)	H700L	probably benign	Het
Gm8356	T	A	14: 17,691,282 (GRCm39)	M128L	probably benign	Het
Golim4	G	T	3: 75,805,442 (GRCm39)		probably null	Het
Grm1	T	G	10: 10,622,403 (GRCm39)	D440A	probably benign	Het
Gtf2a1	A	G	12: 91,529,747 (GRCm39)	V322A	probably benign	Het
Hid1	T	C	11: 115,243,407 (GRCm39)		probably null	Het
Igfals	G	A	17: 25,098,962 (GRCm39)	V18M	possibly damaging	Het
Klk1b24	G	T	7: 43,839,688 (GRCm39)		probably null	Het
Lmbr1	T	C	5: 29,551,850 (GRCm39)		probably benign	Het
Lrrc40	T	C	3: 157,746,194 (GRCm39)	S90P	probably benign	Het
Mapk9	T	C	11: 49,763,663 (GRCm39)	Y185H	probably damaging	Het
Marveld2	C	T	13: 100,748,068 (GRCm39)	G337D	probably damaging	Het
Mga	G	T	2: 119,776,710 (GRCm39)	R1539L	probably damaging	Het
Mllt6	T	A	11: 97,563,442 (GRCm39)	S342T	probably benign	Het
Ms4a6c	T	C	19: 11,449,893 (GRCm39)		probably null	Het
Muc16	T	A	9: 18,558,064 (GRCm39)	H2743L	unknown	Het
Myl10	G	C	5: 136,726,825 (GRCm39)	V70L	probably benign	Het
Or10ag60	T	C	2: 87,438,281 (GRCm39)	F183S	probably damaging	Het
Or2l13	A	G	16: 19,305,753 (GRCm39)	H55R	possibly damaging	Het
Plcd3	T	G	11: 102,962,545 (GRCm39)	K635N	probably damaging	Het
Prkch	T	A	12: 73,632,301 (GRCm39)		probably null	Het
Rb1cc1	T	C	1: 6,344,441 (GRCm39)	V1570A	probably damaging	Het
Rp1	T	C	1: 4,415,704 (GRCm39)	N1803D	probably benign	Het
Rufy3	T	A	5: 88,746,331 (GRCm39)	V72E	probably benign	Het
Secisbp2l	G	A	2: 125,613,452 (GRCm39)	Q181*	probably null	Het
Sgcb	A	G	5: 73,797,188 (GRCm39)	F191L	possibly damaging	Het
Sgsh	T	A	11: 119,237,183 (GRCm39)	D477V	probably damaging	Het
Shoc1	A	T	4: 59,062,286 (GRCm39)	V924E	probably damaging	Het
Slc22a28	C	A	19: 8,048,492 (GRCm39)	S385I	probably benign	Het
Slc26a3	T	C	12: 31,497,787 (GRCm39)	V47A	probably benign	Het
Sltm	T	C	9: 70,481,179 (GRCm39)	S344P	unknown	Het
Sort1	A	G	3: 108,246,141 (GRCm39)	T373A	probably damaging	Het
Stab1	A	G	14: 30,882,274 (GRCm39)	F474L	probably benign	Het
Tbc1d5	G	T	17: 51,224,573 (GRCm39)	A326E	possibly damaging	Het
Tcstv4	A	T	13: 120,770,017 (GRCm39)	K112N	probably damaging	Het
Vwa8	A	C	14: 79,219,674 (GRCm39)		probably null	Het
Wdr70	G	A	15: 7,951,562 (GRCm39)	T425I	probably benign	Het
Zfp30	T	C	7: 29,492,231 (GRCm39)	Y243H	probably benign	Het
Zfp974	A	T	7: 27,611,559 (GRCm39)	N55K	possibly damaging	Het
Zftraf1	A	G	15: 76,530,435 (GRCm39)	L295P	probably damaging	Het

Other mutations in Slco2a1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00418:Slco2a1	APN	9	102,956,640 (GRCm39)	splice site	probably benign
IGL01481:Slco2a1	APN	9	102,947,450 (GRCm39)	missense	probably damaging	1.00
IGL01647:Slco2a1	APN	9	102,947,495 (GRCm39)	missense	possibly damaging	0.57
IGL01885:Slco2a1	APN	9	102,951,629 (GRCm39)	missense	probably damaging	1.00
IGL02150:Slco2a1	APN	9	102,962,017 (GRCm39)	missense	probably damaging	1.00
IGL02508:Slco2a1	APN	9	102,951,615 (GRCm39)	missense	probably benign
IGL02578:Slco2a1	APN	9	102,923,957 (GRCm39)	missense	probably damaging	1.00
IGL02622:Slco2a1	APN	9	102,954,128 (GRCm39)	nonsense	probably null
IGL02898:Slco2a1	APN	9	102,956,805 (GRCm39)	missense	probably damaging	1.00
IGL03101:Slco2a1	APN	9	102,954,205 (GRCm39)	missense	possibly damaging	0.69
PIT4431001:Slco2a1	UTSW	9	102,927,467 (GRCm39)	missense	probably damaging	1.00
R0410:Slco2a1	UTSW	9	102,950,513 (GRCm39)	critical splice donor site	probably null
R0831:Slco2a1	UTSW	9	102,959,533 (GRCm39)	missense	probably damaging	0.99
R0885:Slco2a1	UTSW	9	102,959,582 (GRCm39)	missense	probably damaging	0.98
R1975:Slco2a1	UTSW	9	102,956,653 (GRCm39)	nonsense	probably null
R2095:Slco2a1	UTSW	9	102,954,167 (GRCm39)	missense	probably benign	0.22
R4072:Slco2a1	UTSW	9	102,945,201 (GRCm39)	missense	probably damaging	1.00
R4105:Slco2a1	UTSW	9	102,950,449 (GRCm39)	missense	probably damaging	1.00
R4105:Slco2a1	UTSW	9	102,945,075 (GRCm39)	missense	probably benign	0.01
R4804:Slco2a1	UTSW	9	102,950,383 (GRCm39)	missense	probably damaging	1.00
R4881:Slco2a1	UTSW	9	102,963,031 (GRCm39)	missense	possibly damaging	0.71
R5073:Slco2a1	UTSW	9	102,923,925 (GRCm39)	missense	probably damaging	1.00
R5124:Slco2a1	UTSW	9	102,927,365 (GRCm39)	missense	probably damaging	1.00
R5147:Slco2a1	UTSW	9	102,927,468 (GRCm39)	missense	probably damaging	1.00
R5317:Slco2a1	UTSW	9	102,956,778 (GRCm39)	missense	probably benign	0.01
R5363:Slco2a1	UTSW	9	102,947,462 (GRCm39)	missense	probably damaging	0.99
R5381:Slco2a1	UTSW	9	102,945,213 (GRCm39)	missense	probably damaging	1.00
R5732:Slco2a1	UTSW	9	102,927,455 (GRCm39)	missense	probably damaging	1.00
R5736:Slco2a1	UTSW	9	102,945,029 (GRCm39)	missense	probably benign	0.00
R5924:Slco2a1	UTSW	9	102,923,898 (GRCm39)	nonsense	probably null
R5945:Slco2a1	UTSW	9	102,923,989 (GRCm39)	missense	probably damaging	1.00
R6293:Slco2a1	UTSW	9	102,927,346 (GRCm39)	missense	probably benign	0.30
R6386:Slco2a1	UTSW	9	102,954,187 (GRCm39)	missense	probably benign
R6622:Slco2a1	UTSW	9	102,951,704 (GRCm39)	missense	possibly damaging	0.84
R7325:Slco2a1	UTSW	9	102,962,948 (GRCm39)	splice site	probably null
R8395:Slco2a1	UTSW	9	102,954,239 (GRCm39)	missense	probably benign	0.12
R8985:Slco2a1	UTSW	9	102,949,834 (GRCm39)	critical splice donor site	probably null
R9127:Slco2a1	UTSW	9	102,945,243 (GRCm39)	missense	probably damaging	1.00
R9141:Slco2a1	UTSW	9	102,945,254 (GRCm39)	splice site	probably benign
R9620:Slco2a1	UTSW	9	102,962,065 (GRCm39)	missense	probably damaging	1.00
R9695:Slco2a1	UTSW	9	102,962,139 (GRCm39)	missense	possibly damaging	0.80
Z1088:Slco2a1	UTSW	9	102,956,726 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- ATGGTGACTACTGGGCATGC -3'
(R):5'- CTGGCATTAACAATGGGTCTTTG -3'

Sequencing Primer
(F):5'- GACTACTGGGCATGCTCATTGC -3'
(R):5'- AACAATGGGTCTTTGTCATCCAC -3'

Posted On

2019-10-07