Mutagenetix > Incidental Mutation

Incidental Mutation 'R7436:Dusp10'

580527

Institutional Source

Beutler Lab

Gene Symbol

Dusp10

Ensembl Gene

ENSMUSG00000039384

Gene Name

dual specificity phosphatase 10

Synonyms

MKP5, 2610306G15Rik, MKP-5

MMRRC Submission

045512-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.734) question?

Stock #

R7436 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

183766575-183807833 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 183801418 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 395 (I395T)

Ref Sequence

ENSEMBL: ENSMUSP00000045838 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000048655]

AlphaFold

Q9ESS0

Predicted Effect

probably damaging
Transcript: ENSMUST00000048655
AA Change: I395T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000045838
Gene: ENSMUSG00000039384
AA Change: I395T

Domain	Start	End	E-Value	Type
low complexity region	33	59	N/A	INTRINSIC
low complexity region	92	111	N/A	INTRINSIC
low complexity region	124	142	N/A	INTRINSIC
RHOD	159	283	1.71e-11	SMART
DSPc	322	462	1.43e-54	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

97% (66/68)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAP kinase superfamily, which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAP kinases, different tissue distribution and subcellular localization, and different modes of expression induction by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
PHENOTYPE: Mice homozygous for a knock-out allele display alterations in both innate and adaptive immune responses. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700024B05Rik	A	G	14: 41,805,178 (GRCm39)		probably null	Het
2310034C09Rik	A	G	16: 88,556,242 (GRCm39)	Y152C	probably benign	Het
Acads	C	A	5: 115,249,057 (GRCm39)	Q365H	probably damaging	Het
Akap1	A	G	11: 88,736,354 (GRCm39)	S103P	probably damaging	Het
Ampd1	T	A	3: 102,981,435 (GRCm39)		probably null	Het
Apoa1	T	A	9: 46,141,100 (GRCm39)		probably null	Het
Asb16	A	G	11: 102,163,481 (GRCm39)	D157G	possibly damaging	Het
Bank1	T	C	3: 135,761,561 (GRCm39)	N748D	possibly damaging	Het
BC034090	C	A	1: 155,102,127 (GRCm39)	G46W	probably damaging	Het
Ccdc202	A	T	14: 96,120,027 (GRCm39)	K261N	probably benign	Het
Ccdc73	T	A	2: 104,782,214 (GRCm39)	V190E	probably damaging	Het
Cracd	GCGCGAGGCCGAGAGGCAGGAGGAGGAAGCAAGACAACGCGAGGCCGAGAGGCAGG	GCGCGAGGCCGAGAGGCAGG	5: 77,004,801 (GRCm39)		probably benign	Het
Cyb561a3	A	G	19: 10,559,696 (GRCm39)	Y7C	probably damaging	Het
Cyp2t4	A	T	7: 26,857,668 (GRCm39)	D427V	probably damaging	Het
Cyp4f14	T	C	17: 33,128,131 (GRCm39)	T295A	probably benign	Het
D430041D05Rik	T	C	2: 104,087,447 (GRCm39)	T510A	probably benign	Het
Dennd6a	G	T	14: 26,300,865 (GRCm39)	E26*	probably null	Het
Dzip3	T	A	16: 48,772,352 (GRCm39)	H439L	probably damaging	Het
Heatr5b	T	A	17: 79,075,962 (GRCm39)	D1452V	probably benign	Het
Hsd3b2	A	G	3: 98,619,112 (GRCm39)	F278L	probably benign	Het
Hspg2	T	A	4: 137,242,975 (GRCm39)	M702K	probably damaging	Het
Hydin	T	C	8: 111,310,546 (GRCm39)	F4081L	probably damaging	Het
Ido1	T	G	8: 25,076,932 (GRCm39)	T209P	probably benign	Het
Ift122	C	T	6: 115,903,263 (GRCm39)	R1176C	probably benign	Het
Ipo8	T	C	6: 148,691,303 (GRCm39)	Y689C	probably benign	Het
Macf1	C	T	4: 123,350,436 (GRCm39)	R3809Q	probably benign	Het
Manea	T	C	4: 26,328,228 (GRCm39)	Y271C	probably damaging	Het
Mroh2b	A	G	15: 4,971,036 (GRCm39)	T1014A	probably benign	Het
Nek5	A	G	8: 22,598,056 (GRCm39)	F200L	probably damaging	Het
Nipal1	A	G	5: 72,824,984 (GRCm39)	I226V	probably benign	Het
Nipsnap3a	A	T	4: 52,994,159 (GRCm39)	N80I	probably damaging	Het
Nmur1	G	A	1: 86,314,100 (GRCm39)	P389S	probably benign	Het
Or10d5	G	T	9: 39,861,349 (GRCm39)	C239*	probably null	Het
Or1p1	T	C	11: 74,179,511 (GRCm39)	L13P	possibly damaging	Het
Or8u10	T	C	2: 85,915,251 (GRCm39)	Y290C	probably damaging	Het
Patl2	A	C	2: 121,958,006 (GRCm39)	V84G	probably benign	Het
Pcdhb4	T	C	18: 37,442,328 (GRCm39)	V546A	probably damaging	Het
Phf20l1	G	T	15: 66,469,599 (GRCm39)	S168I	possibly damaging	Het
Phgdh	T	C	3: 98,247,045 (GRCm39)	N35S	probably benign	Het
Pigw	A	T	11: 84,768,789 (GRCm39)	M180K	probably damaging	Het
Pkhd1	G	T	1: 20,270,925 (GRCm39)	H3209Q	probably benign	Het
Plbd2	A	G	5: 120,624,861 (GRCm39)	F436L	probably damaging	Het
Plch2	T	C	4: 155,068,553 (GRCm39)	T1358A	probably benign	Het
Poll	C	T	19: 45,541,496 (GRCm39)	V491M	probably damaging	Het
Polr1e	A	G	4: 45,024,553 (GRCm39)		probably null	Het
Ppp1r35	G	A	5: 137,778,279 (GRCm39)	W258*	probably null	Het
Ptpdc1	A	T	13: 48,740,142 (GRCm39)	F430I	probably benign	Het
Ptprh	T	C	7: 4,555,742 (GRCm39)	E739G	probably damaging	Het
Ramp2	T	C	11: 101,138,765 (GRCm39)	V148A	possibly damaging	Het
Rapgef6	T	C	11: 54,501,747 (GRCm39)		probably null	Het
Rbm5	A	G	9: 107,627,593 (GRCm39)		probably null	Het
Rnf39	A	T	17: 37,254,241 (GRCm39)	S88C	probably benign	Het
Rpl12	A	G	2: 32,853,836 (GRCm39)	I155V	probably benign	Het
Scart2	A	T	7: 139,841,520 (GRCm39)	T275S	probably benign	Het
Senp5	C	T	16: 31,794,847 (GRCm39)	E596K	unknown	Het
Serpinb9d	A	G	13: 33,379,916 (GRCm39)	Y85C	probably benign	Het
Serpinf1	T	A	11: 75,307,142 (GRCm39)	Y65F	probably benign	Het
Snrnp200	G	A	2: 127,068,404 (GRCm39)		probably null	Het
Spatc1	A	T	15: 76,152,568 (GRCm39)	Q66L	probably benign	Het
Tet3	TGGCCCAGGCCCAGGC	TGGCCCAGGCCCAGGCCCAGGC	6: 83,345,211 (GRCm39)		probably benign	Het
Traf3ip1	A	G	1: 91,439,110 (GRCm39)	D342G	probably benign	Het
Txk	T	A	5: 72,853,922 (GRCm39)	T472S	probably damaging	Het
Ube2j2	T	C	4: 156,041,788 (GRCm39)	V249A	probably damaging	Het
Utrn	A	C	10: 12,315,535 (GRCm39)	N3025K	possibly damaging	Het
Vmn1r17	T	A	6: 57,337,862 (GRCm39)	M168L	probably benign	Het
Vmn1r77	T	A	7: 11,775,694 (GRCm39)	S157T	probably benign	Het
Zfp977	A	G	7: 42,229,884 (GRCm39)	S214P	probably benign	Het
Zscan10	T	C	17: 23,828,979 (GRCm39)	L507P	possibly damaging	Het

Other mutations in Dusp10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00335:Dusp10	APN	1	183,801,328 (GRCm39)	missense	probably benign	0.00
IGL01094:Dusp10	APN	1	183,769,697 (GRCm39)	splice site	probably null
IGL01380:Dusp10	APN	1	183,801,211 (GRCm39)	missense	possibly damaging	0.93
FR4449:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
FR4548:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
FR4737:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
FR4976:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
LCD18:Dusp10	UTSW	1	183,769,253 (GRCm39)	missense	probably damaging	1.00
R0369:Dusp10	UTSW	1	183,801,253 (GRCm39)	missense	probably damaging	1.00
R0433:Dusp10	UTSW	1	183,801,393 (GRCm39)	missense	probably damaging	1.00
R0464:Dusp10	UTSW	1	183,801,273 (GRCm39)	missense	probably benign	0.01
R1112:Dusp10	UTSW	1	183,769,097 (GRCm39)	missense	probably damaging	0.98
R1474:Dusp10	UTSW	1	183,769,645 (GRCm39)	splice site	probably null
R1667:Dusp10	UTSW	1	183,769,055 (GRCm39)	missense	probably damaging	1.00
R1719:Dusp10	UTSW	1	183,769,422 (GRCm39)	missense	probably benign	0.22
R1899:Dusp10	UTSW	1	183,801,377 (GRCm39)	missense	possibly damaging	0.64
R5238:Dusp10	UTSW	1	183,769,210 (GRCm39)	missense	possibly damaging	0.94
R5277:Dusp10	UTSW	1	183,769,204 (GRCm39)	missense	possibly damaging	0.94
R5742:Dusp10	UTSW	1	183,769,853 (GRCm39)	splice site	probably null
R5948:Dusp10	UTSW	1	183,801,073 (GRCm39)	missense	probably benign
R6890:Dusp10	UTSW	1	183,801,393 (GRCm39)	missense	probably damaging	1.00
R6969:Dusp10	UTSW	1	183,801,085 (GRCm39)	missense	probably damaging	1.00
R7007:Dusp10	UTSW	1	183,769,414 (GRCm39)	missense	probably benign	0.22
R7033:Dusp10	UTSW	1	183,769,802 (GRCm39)	missense	possibly damaging	0.94
R7447:Dusp10	UTSW	1	183,801,153 (GRCm39)	missense	probably benign
R7479:Dusp10	UTSW	1	183,769,617 (GRCm39)	missense	probably damaging	0.99
R7572:Dusp10	UTSW	1	183,806,506 (GRCm39)	missense	probably damaging	1.00
R8191:Dusp10	UTSW	1	183,769,749 (GRCm39)	missense	possibly damaging	0.89
R8201:Dusp10	UTSW	1	183,769,202 (GRCm39)	missense	possibly damaging	0.51
R9429:Dusp10	UTSW	1	183,801,091 (GRCm39)	missense	probably benign	0.01
R9466:Dusp10	UTSW	1	183,769,234 (GRCm39)	missense	probably damaging	1.00
R9593:Dusp10	UTSW	1	183,806,643 (GRCm39)	missense	probably damaging	0.99
Z1177:Dusp10	UTSW	1	183,801,189 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TCCTGTTCCTCGGCAATGAG -3'
(R):5'- CAATTATGCCCATCCAGGTAGTTC -3'

Sequencing Primer
(F):5'- TTCCTCGGCAATGAGCAGGATG -3'
(R):5'- CCATCCAGGTAGTTCTGAGAC -3'

Posted On

2019-10-07