Incidental Mutation 'R7233:Lats2'
ID 580592
Institutional Source Beutler Lab
Gene Symbol Lats2
Ensembl Gene ENSMUSG00000021959
Gene Name large tumor suppressor 2
Synonyms
MMRRC Submission 045343-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R7233 (G1)
Quality Score 225.009
Status Validated
Chromosome 14
Chromosomal Location 57927119-57983669 bp(-) (GRCm39)
Type of Mutation splice site (6 bp from exon)
DNA Base Change (assembly) A to T at 57960151 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000038982 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000022531] [ENSMUST00000038381] [ENSMUST00000038381] [ENSMUST00000077981] [ENSMUST00000173990] [ENSMUST00000174166] [ENSMUST00000174213] [ENSMUST00000174694]
AlphaFold Q7TSJ6
Predicted Effect probably benign
Transcript: ENSMUST00000022531
SMART Domains Protein: ENSMUSP00000022531
Gene: ENSMUSG00000021959

DomainStartEndE-ValueType
PDB:2COS|A 91 138 3e-20 PDB
low complexity region 210 223 N/A INTRINSIC
low complexity region 401 408 N/A INTRINSIC
low complexity region 437 444 N/A INTRINSIC
low complexity region 471 482 N/A INTRINSIC
low complexity region 517 529 N/A INTRINSIC
S_TKc 626 931 2.94e-94 SMART
S_TK_X 932 1002 1.21e-3 SMART
Predicted Effect probably null
Transcript: ENSMUST00000038381
Predicted Effect probably null
Transcript: ENSMUST00000038381
Predicted Effect probably benign
Transcript: ENSMUST00000077981
Predicted Effect probably benign
Transcript: ENSMUST00000173990
SMART Domains Protein: ENSMUSP00000133976
Gene: ENSMUSG00000021959

DomainStartEndE-ValueType
PDB:2COS|A 91 138 8e-22 PDB
low complexity region 210 223 N/A INTRINSIC
low complexity region 401 408 N/A INTRINSIC
low complexity region 437 444 N/A INTRINSIC
low complexity region 471 482 N/A INTRINSIC
low complexity region 517 529 N/A INTRINSIC
S_TKc 626 893 7.75e-71 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000174166
SMART Domains Protein: ENSMUSP00000133379
Gene: ENSMUSG00000021959

DomainStartEndE-ValueType
PDB:2COS|A 91 114 5e-7 PDB
Predicted Effect probably benign
Transcript: ENSMUST00000174213
SMART Domains Protein: ENSMUSP00000134321
Gene: ENSMUSG00000021959

DomainStartEndE-ValueType
PDB:2COS|A 91 114 2e-6 PDB
Predicted Effect probably benign
Transcript: ENSMUST00000174694
SMART Domains Protein: ENSMUSP00000133680
Gene: ENSMUSG00000114942

DomainStartEndE-ValueType
PDB:2COS|A 91 138 7e-22 PDB
low complexity region 210 223 N/A INTRINSIC
low complexity region 401 408 N/A INTRINSIC
low complexity region 437 444 N/A INTRINSIC
low complexity region 471 482 N/A INTRINSIC
low complexity region 517 529 N/A INTRINSIC
Pfam:Pkinase 626 792 2.2e-38 PFAM
Pfam:Pkinase_Tyr 626 795 2.8e-21 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.8%
Validation Efficiency 100% (75/75)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mice display embryonic lethality with decreased cell proliferation, chromosomal instability, atrial hyperplasia, ventricular hypoplasia, delayed embryonic development, an irregular kinked neural tube, and hemorrhages. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 76 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acox3 A G 5: 35,762,641 (GRCm39) K505R probably benign Het
Arhgap24 A G 5: 103,026,367 (GRCm39) K263E probably benign Het
Ark2n C A 18: 77,722,882 (GRCm39) V385F probably damaging Het
Armc2 A T 10: 41,799,800 (GRCm39) V686D probably damaging Het
Bhmt T A 13: 93,758,025 (GRCm39) K229* probably null Het
Birc2 A T 9: 7,827,009 (GRCm39) C326S probably damaging Het
Blzf1 A T 1: 164,123,512 (GRCm39) probably null Het
Camsap3 T A 8: 3,650,371 (GRCm39) F223Y probably damaging Het
Ccdc126 C T 6: 49,316,775 (GRCm39) T85M probably damaging Het
Ccng2 C G 5: 93,421,202 (GRCm39) S237R probably benign Het
Ces2h T A 8: 105,744,088 (GRCm39) C279S probably damaging Het
Cfap44 T A 16: 44,242,771 (GRCm39) L725Q probably damaging Het
Cibar2 A G 8: 120,898,661 (GRCm39) L131P probably damaging Het
Clip1 T C 5: 123,749,922 (GRCm39) E987G probably damaging Het
Cog1 G T 11: 113,540,556 (GRCm39) R57L probably damaging Het
Ctdspl T C 9: 118,849,114 (GRCm39) probably null Het
Dnaaf9 C A 2: 130,648,708 (GRCm39) R258L unknown Het
Dnttip2 T C 3: 122,070,039 (GRCm39) V418A probably benign Het
Dop1a G A 9: 86,403,749 (GRCm39) A186T probably benign Het
Efcab3 T C 11: 104,730,669 (GRCm39) S1966P possibly damaging Het
Engase T A 11: 118,373,827 (GRCm39) V323E probably damaging Het
Farsb A G 1: 78,447,718 (GRCm39) probably null Het
Fpr2 G T 17: 18,113,766 (GRCm39) W254L probably damaging Het
Frmd3 A C 4: 73,932,023 (GRCm39) H6P probably benign Het
Fscn1 C T 5: 142,956,029 (GRCm39) S366L possibly damaging Het
Galt T A 4: 41,758,267 (GRCm39) I344N probably benign Het
Gfra4 A T 2: 130,883,037 (GRCm39) V194E probably damaging Het
Golgb1 T C 16: 36,735,120 (GRCm39) S1497P possibly damaging Het
Igfn1 A C 1: 135,897,873 (GRCm39) S898A probably benign Het
Igkv1-135 T C 6: 67,587,332 (GRCm39) S68P probably benign Het
Lama2 A T 10: 27,107,659 (GRCm39) C784S probably damaging Het
Lars2 G T 9: 123,241,019 (GRCm39) G229* probably null Het
Lgr6 A C 1: 134,928,214 (GRCm39) probably null Het
Lrp1 A T 10: 127,430,930 (GRCm39) I373N probably damaging Het
Maml2 A T 9: 13,532,067 (GRCm39) H427L Het
Mansc1 A G 6: 134,598,806 (GRCm39) V37A probably damaging Het
Map4k3 A G 17: 80,905,077 (GRCm39) V738A possibly damaging Het
Mastl A G 2: 23,023,670 (GRCm39) I351T probably benign Het
Mcmdc2 T G 1: 10,002,408 (GRCm39) probably null Het
Mrgprd A G 7: 144,875,672 (GRCm39) D181G possibly damaging Het
Msantd5f9 G T 4: 73,835,808 (GRCm39) L219M probably benign Het
Msra T C 14: 64,360,714 (GRCm39) Y209C probably damaging Het
Musk A G 4: 58,373,307 (GRCm39) E759G possibly damaging Het
Nab1 T C 1: 52,498,378 (GRCm39) *487W probably null Het
Or2z9 A G 8: 72,853,900 (GRCm39) T99A probably benign Het
Or5p66 T A 7: 107,885,923 (GRCm39) T137S probably benign Het
Pkdrej A G 15: 85,705,349 (GRCm39) S196P probably damaging Het
Pln A G 10: 53,220,008 (GRCm39) T17A probably damaging Het
Polrmt A T 10: 79,581,619 (GRCm39) probably null Het
Ppp1r14a T A 7: 28,988,949 (GRCm39) Y64N probably damaging Het
Ppp1r9a A T 6: 5,134,804 (GRCm39) H959L probably benign Het
Pramel42 A T 5: 94,685,524 (GRCm39) M395L probably benign Het
Prmt7 A G 8: 106,946,642 (GRCm39) T75A probably damaging Het
Prom1 T C 5: 44,194,816 (GRCm39) S319G possibly damaging Het
Prtg G A 9: 72,819,273 (GRCm39) G1089S probably benign Het
Ptprd T C 4: 75,978,020 (GRCm39) D596G probably benign Het
Rbm12 C T 2: 155,937,894 (GRCm39) G793S unknown Het
Rora A T 9: 69,104,804 (GRCm39) R43* probably null Het
Sez6 T C 11: 77,863,963 (GRCm39) Y482H probably damaging Het
Skint2 A T 4: 112,483,122 (GRCm39) N176Y probably damaging Het
Slc44a2 G T 9: 21,259,445 (GRCm39) probably null Het
Sox6 A G 7: 115,089,044 (GRCm39) V606A possibly damaging Het
Spa17 T C 9: 37,514,587 (GRCm39) probably null Het
Syne1 A C 10: 5,252,160 (GRCm39) L2498R probably damaging Het
Synpo2 T G 3: 122,911,333 (GRCm39) H104P probably benign Het
Tapbp G T 17: 34,138,943 (GRCm39) A46S probably damaging Het
Tbc1d30 C A 10: 121,107,962 (GRCm39) R480L probably benign Het
Tshz1 T C 18: 84,032,944 (GRCm39) D488G possibly damaging Het
Ulk1 A G 5: 110,956,908 (GRCm39) L70P probably damaging Het
Zcchc17 T A 4: 130,221,116 (GRCm39) D145V probably damaging Het
Zfp318 T A 17: 46,716,978 (GRCm39) L1037M probably damaging Het
Zfp324 C T 7: 12,704,524 (GRCm39) Q238* probably null Het
Zfp874a A G 13: 67,590,776 (GRCm39) Y303H possibly damaging Het
Zfp975 T C 7: 42,311,918 (GRCm39) K232E probably benign Het
Zfp982 T A 4: 147,597,718 (GRCm39) N358K probably benign Het
Zmiz1 C T 14: 25,650,092 (GRCm39) P417S possibly damaging Het
Other mutations in Lats2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00272:Lats2 APN 14 57,929,026 (GRCm39) missense probably benign 0.09
IGL02104:Lats2 APN 14 57,971,469 (GRCm39) missense probably damaging 1.00
IGL02173:Lats2 APN 14 57,934,717 (GRCm39) missense probably damaging 1.00
IGL02377:Lats2 APN 14 57,929,052 (GRCm39) missense probably damaging 1.00
IGL02995:Lats2 APN 14 57,937,805 (GRCm39) missense probably damaging 1.00
Morpheus UTSW 14 57,933,591 (GRCm39) missense probably damaging 1.00
PIT4472001:Lats2 UTSW 14 57,936,814 (GRCm39) nonsense probably null
R0653:Lats2 UTSW 14 57,937,653 (GRCm39) nonsense probably null
R0780:Lats2 UTSW 14 57,928,753 (GRCm39) missense probably damaging 1.00
R1129:Lats2 UTSW 14 57,937,790 (GRCm39) missense possibly damaging 0.71
R1851:Lats2 UTSW 14 57,934,912 (GRCm39) missense probably damaging 1.00
R1882:Lats2 UTSW 14 57,934,811 (GRCm39) missense probably damaging 1.00
R2184:Lats2 UTSW 14 57,929,016 (GRCm39) missense probably damaging 0.99
R3498:Lats2 UTSW 14 57,959,923 (GRCm39) missense possibly damaging 0.95
R3692:Lats2 UTSW 14 57,928,998 (GRCm39) missense probably damaging 1.00
R4212:Lats2 UTSW 14 57,933,712 (GRCm39) missense possibly damaging 0.82
R4357:Lats2 UTSW 14 57,936,840 (GRCm39) missense probably damaging 1.00
R4962:Lats2 UTSW 14 57,937,049 (GRCm39) missense probably damaging 1.00
R5394:Lats2 UTSW 14 57,928,810 (GRCm39) missense probably benign 0.10
R5477:Lats2 UTSW 14 57,937,010 (GRCm39) missense probably benign 0.00
R5729:Lats2 UTSW 14 57,960,192 (GRCm39) missense probably benign 0.04
R5802:Lats2 UTSW 14 57,931,875 (GRCm39) missense probably damaging 0.99
R5931:Lats2 UTSW 14 57,933,588 (GRCm39) missense probably damaging 1.00
R6016:Lats2 UTSW 14 57,971,632 (GRCm39) missense probably damaging 1.00
R6376:Lats2 UTSW 14 57,959,966 (GRCm39) missense probably benign 0.00
R6624:Lats2 UTSW 14 57,931,769 (GRCm39) critical splice donor site probably null
R6638:Lats2 UTSW 14 57,936,822 (GRCm39) missense probably damaging 1.00
R6846:Lats2 UTSW 14 57,933,591 (GRCm39) missense probably damaging 1.00
R7198:Lats2 UTSW 14 57,934,582 (GRCm39) missense probably damaging 1.00
R7883:Lats2 UTSW 14 57,934,657 (GRCm39) missense probably damaging 1.00
R8081:Lats2 UTSW 14 57,937,968 (GRCm39) missense probably damaging 1.00
R8356:Lats2 UTSW 14 57,934,867 (GRCm39) missense probably damaging 1.00
R8508:Lats2 UTSW 14 57,960,162 (GRCm39) missense probably benign 0.08
R8536:Lats2 UTSW 14 57,940,495 (GRCm39) missense probably damaging 1.00
R8767:Lats2 UTSW 14 57,931,781 (GRCm39) missense probably damaging 1.00
R9579:Lats2 UTSW 14 57,937,191 (GRCm39) missense probably damaging 1.00
R9643:Lats2 UTSW 14 57,936,875 (GRCm39) missense possibly damaging 0.94
Predicted Primers PCR Primer
(F):5'- AGAATCAAGCCCTAGTATTATGGAC -3'
(R):5'- TAACAGAGGGATGGTGGCTTC -3'

Sequencing Primer
(F):5'- TCCGTGCTTCAGGTACAAG -3'
(R):5'- GACAGTCTGTGCCCTTAACTATGAG -3'
Posted On 2019-10-10