Mutagenetix > Incidental Mutation

Incidental Mutation 'R0633:Lpar5'

58067

Institutional Source

Beutler Lab

Gene Symbol

Lpar5

Ensembl Gene

ENSMUSG00000067714

Gene Name

lysophosphatidic acid receptor 5

Synonyms

LPA5, LOC381810, Gpr92, GPR93

MMRRC Submission

038822-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R0633 (G1)

Quality Score

222

Status

Not validated

Chromosome

Chromosomal Location

125067920-125082472 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to C at 125081991 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Serine at position 225 (Y225S)

Ref Sequence

ENSEMBL: ENSMUSP00000132511 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088292] [ENSMUST00000140346] [ENSMUST00000171989]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000088292
AA Change: Y225S

PolyPhen 2 Score 0.255 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000085630
Gene: ENSMUSG00000067714
AA Change: Y225S

Domain	Start	End	E-Value	Type
low complexity region	15	27	N/A	INTRINSIC
Pfam:7tm_1	55	313	7.4e-40	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000140346

SMART Domains

Protein: ENSMUSP00000119904
Gene: ENSMUSG00000067714

Domain	Start	End	E-Value	Type
low complexity region	15	27	N/A	INTRINSIC
Pfam:7tm_1	55	164	1.5e-30	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000171989
AA Change: Y225S

PolyPhen 2 Score 0.255 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000132511
Gene: ENSMUSG00000067714
AA Change: Y225S

Domain	Start	End	E-Value	Type
low complexity region	15	27	N/A	INTRINSIC
Pfam:7tm_1	55	313	1.1e-48	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203956

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.4%
20x: 94.7%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the rhodopsin class of G protein-coupled transmembrane receptors. This protein transmits extracellular signals from lysophosphatidic acid to cells through heterotrimeric G proteins and mediates numerous cellular processes. Many G protein receptors serve as targets for pharmaceutical drugs. Transcript variants of this gene have been described.[provided by RefSeq, Dec 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit resistance to neuropathic pain and myelin sheath alterations. Mice homozygous for a different targeted allele exhibit decreased nociception sensitivity, decreased anxiety-related response and enhanced coordination and spatial learning. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 77 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921530L21Rik	T	G	14: 95,881,943 (GRCm38)	N45K	probably damaging	Het
Adamts8	A	T	9: 30,943,511 (GRCm38)	R18S	probably damaging	Het
Adgb	G	A	10: 10,391,729 (GRCm38)	A923V	probably benign	Het
Aldh1a3	A	G	7: 66,400,222 (GRCm38)	V416A	probably damaging	Het
Alox5	C	T	6: 116,420,384 (GRCm38)	G280R	probably damaging	Het
Anapc5	A	T	5: 122,800,632 (GRCm38)	Y360N	probably damaging	Het
Apbb1	C	T	7: 105,558,963 (GRCm38)	V685I	probably damaging	Het
Apc2	C	A	10: 80,307,455 (GRCm38)	A463E	probably damaging	Het
Arhgap21	C	T	2: 20,855,387 (GRCm38)	W1170*	probably null	Het
Atat1	G	A	17: 35,901,423 (GRCm38)	R305C	probably damaging	Het
Brd8dc	A	G	18: 34,586,266 (GRCm38)	V167A	possibly damaging	Het
Cars2	T	C	8: 11,550,511 (GRCm38)	D56G	probably benign	Het
Cdc42bpb	T	C	12: 111,345,555 (GRCm38)	I108V	probably damaging	Het
Cftr	T	A	6: 18,305,980 (GRCm38)	I1255K	probably damaging	Het
Ckap5	T	C	2: 91,550,743 (GRCm38)	L148P	probably damaging	Het
Cntn4	A	G	6: 106,679,248 (GRCm38)		probably null	Het
Cpe	G	A	8: 64,609,203 (GRCm38)	P273L	probably damaging	Het
Cpsf7	A	G	19: 10,531,782 (GRCm38)	D19G	probably benign	Het
Ddx25	C	A	9: 35,545,972 (GRCm38)	R349L	probably damaging	Het
Depdc7	T	C	2: 104,722,881 (GRCm38)	D446G	probably benign	Het
Det1	T	A	7: 78,843,935 (GRCm38)	N107I	probably benign	Het
Dock6	A	T	9: 21,844,417 (GRCm38)	D170E	probably benign	Het
Dvl1	C	T	4: 155,858,295 (GRCm38)	L673F	probably damaging	Het
Gucy1b1	A	T	3: 82,045,460 (GRCm38)	I222K	probably benign	Het
Hfm1	T	C	5: 106,917,601 (GRCm38)	T71A	possibly damaging	Het
Ikzf1	A	G	11: 11,769,223 (GRCm38)	E310G	probably damaging	Het
Impg1	T	C	9: 80,394,155 (GRCm38)	E163G	possibly damaging	Het
Itpr2	G	T	6: 146,374,456 (GRCm38)	H426Q	probably damaging	Het
Itpripl2	C	T	7: 118,490,256 (GRCm38)	G360D	probably benign	Het
Kif14	C	T	1: 136,527,305 (GRCm38)	R1572C	probably damaging	Het
L3mbtl3	A	T	10: 26,302,685 (GRCm38)	H568Q	unknown	Het
Lgi2	A	G	5: 52,554,460 (GRCm38)	Y173H	probably damaging	Het
Lpin3	A	G	2: 160,903,974 (GRCm38)	H675R	probably damaging	Het
Lrp2	C	A	2: 69,448,120 (GRCm38)	G3963V	probably damaging	Het
Man1a2	G	T	3: 100,684,575 (GRCm38)	D13E	possibly damaging	Het
Map1a	T	C	2: 121,308,014 (GRCm38)	V2753A	probably damaging	Het
Mitf	C	A	6: 98,003,904 (GRCm38)	N97K	probably damaging	Het
Msh2	A	G	17: 87,672,810 (GRCm38)		probably null	Het
Msr1	T	C	8: 39,620,000 (GRCm38)	E170G	probably damaging	Het
Myrip	C	A	9: 120,388,236 (GRCm38)	R79S	probably damaging	Het
Nek10	G	A	14: 14,857,782 (GRCm38)		probably null	Het
Neto1	C	T	18: 86,404,729 (GRCm38)	R104*	probably null	Het
Nom1	A	C	5: 29,451,100 (GRCm38)	K821T	probably damaging	Het
Nrxn1	A	G	17: 90,704,181 (GRCm38)	V340A	probably damaging	Het
Nxpe4	A	T	9: 48,396,597 (GRCm38)	I334F	probably benign	Het
Or1e23	A	G	11: 73,516,927 (GRCm38)	S91P	probably benign	Het
Or2ag1	T	C	7: 106,713,977 (GRCm38)	K235E	probably benign	Het
Or4a74	T	C	2: 89,609,374 (GRCm38)	M243V	probably benign	Het
Or5al7	T	A	2: 86,162,091 (GRCm38)	N286I	probably damaging	Het
Or5b124	T	C	19: 13,633,336 (GRCm38)	V75A	probably damaging	Het
Or8k27	C	T	2: 86,445,129 (GRCm38)	M284I	probably benign	Het
Padi4	A	G	4: 140,757,585 (GRCm38)	S322P	probably damaging	Het
Peli3	A	G	19: 4,941,782 (GRCm38)	Y44H	probably damaging	Het
Prdm4	A	G	10: 85,907,903 (GRCm38)	S163P	probably damaging	Het
Prom2	T	C	2: 127,539,525 (GRCm38)	D227G	probably benign	Het
Ptgfr	C	T	3: 151,801,763 (GRCm38)	R321H	probably benign	Het
Resf1	A	T	6: 149,325,701 (GRCm38)	I82L	probably benign	Het
Rgs3	G	A	4: 62,625,906 (GRCm38)	R136H	probably damaging	Het
Rgsl1	T	G	1: 153,844,107 (GRCm38)	N3T	possibly damaging	Het
Rif1	T	C	2: 52,112,563 (GRCm38)	S2010P	probably benign	Het
Rngtt	T	C	4: 33,368,690 (GRCm38)	F408L	probably damaging	Het
Rtn3	T	G	19: 7,457,593 (GRCm38)	T326P	probably benign	Het
Slc18b1	A	C	10: 23,806,038 (GRCm38)	M167L	probably benign	Het
Slc22a26	A	G	19: 7,788,210 (GRCm38)		probably null	Het
Slitrk6	T	C	14: 110,751,885 (GRCm38)	D130G	probably damaging	Het
Snap47	A	G	11: 59,428,613 (GRCm38)	V233A	probably benign	Het
Sumf1	A	C	6: 108,144,671 (GRCm38)	Y158D	probably damaging	Het
Tbc1d15	A	T	10: 115,220,310 (GRCm38)	H252Q	probably benign	Het
Thsd7b	T	C	1: 130,188,526 (GRCm38)	S1339P	possibly damaging	Het
Tmem45a2	T	C	16: 57,049,414 (GRCm38)	I56V	probably benign	Het
Ttc21b	A	G	2: 66,236,233 (GRCm38)	S359P	probably benign	Het
Ttc27	T	C	17: 74,729,977 (GRCm38)	I215T	probably benign	Het
Ttn	C	T	2: 76,724,195 (GRCm38)	V30759I	possibly damaging	Het
Vdac3	T	C	8: 22,580,388 (GRCm38)	N168S	probably damaging	Het
Wdr7	T	C	18: 63,865,300 (GRCm38)	V1106A	probably benign	Het
Wrap73	T	A	4: 154,142,491 (GRCm38)	F16Y	probably damaging	Het
Zfat	C	A	15: 68,180,803 (GRCm38)	D381Y	probably damaging	Het

Other mutations in Lpar5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01719:Lpar5	APN	6	125,082,006 (GRCm38)	missense	possibly damaging	0.94
IGL01830:Lpar5	APN	6	125,081,822 (GRCm38)	missense	probably benign	0.01
IGL01975:Lpar5	APN	6	125,081,787 (GRCm38)	missense	probably damaging	0.99
IGL02021:Lpar5	APN	6	125,081,992 (GRCm38)	nonsense	probably null
IGL02718:Lpar5	APN	6	125,082,244 (GRCm38)	missense	probably damaging	1.00
IGL03027:Lpar5	APN	6	125,082,240 (GRCm38)	missense	probably damaging	1.00
IGL03300:Lpar5	APN	6	125,082,240 (GRCm38)	missense	probably damaging	1.00
F5770:Lpar5	UTSW	6	125,081,727 (GRCm38)	missense	possibly damaging	0.88
R1639:Lpar5	UTSW	6	125,081,601 (GRCm38)	missense	probably damaging	1.00
R1822:Lpar5	UTSW	6	125,081,415 (GRCm38)	missense	possibly damaging	0.76
R2227:Lpar5	UTSW	6	125,081,135 (GRCm38)	critical splice acceptor site	probably null
R4019:Lpar5	UTSW	6	125,081,675 (GRCm38)	missense	probably damaging	1.00
R4288:Lpar5	UTSW	6	125,081,864 (GRCm38)	missense	probably benign	0.00
R4705:Lpar5	UTSW	6	125,082,207 (GRCm38)	missense	possibly damaging	0.64
R4787:Lpar5	UTSW	6	125,082,498 (GRCm38)	splice site	probably null
R5027:Lpar5	UTSW	6	125,082,147 (GRCm38)	missense	possibly damaging	0.69
R6114:Lpar5	UTSW	6	125,081,676 (GRCm38)	missense	probably damaging	1.00
R7197:Lpar5	UTSW	6	125,082,384 (GRCm38)	missense	probably benign	0.00
R7779:Lpar5	UTSW	6	125,082,244 (GRCm38)	missense	probably damaging	1.00
R8193:Lpar5	UTSW	6	125,081,339 (GRCm38)	missense	probably benign
R8264:Lpar5	UTSW	6	125,081,502 (GRCm38)	missense	probably damaging	1.00
R9460:Lpar5	UTSW	6	125,081,271 (GRCm38)	start gained	probably benign
R9628:Lpar5	UTSW	6	125,081,985 (GRCm38)	missense	probably damaging	0.96
V7580:Lpar5	UTSW	6	125,081,727 (GRCm38)	missense	possibly damaging	0.88
V7581:Lpar5	UTSW	6	125,081,727 (GRCm38)	missense	possibly damaging	0.88
V7582:Lpar5	UTSW	6	125,081,727 (GRCm38)	missense	possibly damaging	0.88
Z1176:Lpar5	UTSW	6	125,082,072 (GRCm38)	missense	probably damaging	1.00
Z1176:Lpar5	UTSW	6	125,081,379 (GRCm38)	missense	possibly damaging	0.92
Z1177:Lpar5	UTSW	6	125,082,018 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTCTGATGCTCATCAACGTGGACC -3'
(R):5'- AACCCATATACAGCCAGCGTGGAG -3'

Sequencing Primer
(F):5'- TCAACGTGGACCGCTATGC -3'
(R):5'- CCAGCGTGGAGTTATAGGGC -3'

Posted On

2013-07-11