Mutagenetix > Incidental Mutation

Incidental Mutation 'R7495:Vmn2r49'

581034

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r49

Ensembl Gene

ENSMUSG00000096180

Gene Name

vomeronasal 2, receptor 49

Synonyms

MMRRC Submission

045568-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.108) question?

Stock #

R7495 (G1)

Quality Score

92.0077

Status

Not validated

Chromosome

Chromosomal Location

9710172-9726066 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 9710826 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 635 (C635*)

Ref Sequence

ENSEMBL: ENSMUSP00000104190 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000108550]

AlphaFold

D3Z6L3

Predicted Effect

probably null
Transcript: ENSMUST00000108550
AA Change: C635*

SMART Domains

Protein: ENSMUSP00000104190
Gene: ENSMUSG00000096180
AA Change: C635*

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	469	1.4e-33	PFAM
Pfam:NCD3G	512	565	5.1e-21	PFAM
Pfam:7tm_3	598	833	3.5e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1b	A	G	5: 8,915,871 (GRCm39)	E1251G	probably damaging	Het
Apba1	T	C	19: 23,913,963 (GRCm39)		probably null	Het
Arap2	C	T	5: 62,833,893 (GRCm39)	S858N	possibly damaging	Het
Capn10	T	C	1: 92,871,092 (GRCm39)	F301L	probably damaging	Het
Catip	T	C	1: 74,401,851 (GRCm39)	W9R	probably benign	Het
Cfap46	A	G	7: 139,183,112 (GRCm39)		probably null	Het
Cpq	C	T	15: 33,302,586 (GRCm39)	R246C	probably damaging	Het
Dgkh	A	G	14: 78,816,239 (GRCm39)	S1067P	probably benign	Het
Dpp3	T	C	19: 4,967,941 (GRCm39)	E316G	probably damaging	Het
Dpp9	T	C	17: 56,502,044 (GRCm39)	Q508R	probably benign	Het
Frem2	T	C	3: 53,424,258 (GRCm39)	N3060D	probably benign	Het
Fstl5	A	G	3: 76,615,099 (GRCm39)	Y720C	possibly damaging	Het
Gm28168	T	C	1: 117,875,637 (GRCm39)	S89P	possibly damaging	Het
Hk2	C	T	6: 82,704,346 (GRCm39)	G900E	probably damaging	Het
Hrh1	A	C	6: 114,457,634 (GRCm39)	D305A	probably benign	Het
Htt	C	T	5: 34,968,821 (GRCm39)	S435L	probably benign	Het
Ice2	G	T	9: 69,323,511 (GRCm39)	V669L	probably benign	Het
Klk1b27	G	A	7: 43,705,500 (GRCm39)	V191I	probably benign	Het
Med12l	A	G	3: 59,152,194 (GRCm39)	K993R	probably damaging	Het
Nfe2l1	A	G	11: 96,710,622 (GRCm39)	Y536H	probably damaging	Het
Nr4a2	T	C	2: 57,002,171 (GRCm39)	D94G	possibly damaging	Het
Oit3	T	C	10: 59,259,765 (GRCm39)	D546G	possibly damaging	Het
Paxbp1	T	C	16: 90,813,837 (GRCm39)	T847A	probably damaging	Het
Pde12	A	T	14: 26,389,994 (GRCm39)	N238K	probably benign	Het
Pfkfb3	A	C	2: 11,487,312 (GRCm39)	I366S	probably damaging	Het
Ptma	GGAAGAAG	GGAAGAAGAAG	1: 86,457,261 (GRCm39)		probably benign	Het
Ptprb	C	A	10: 116,177,353 (GRCm39)	Q1018K	probably benign	Het
Rxfp3	C	A	15: 11,036,011 (GRCm39)	G454C	probably damaging	Het
Scn5a	A	G	9: 119,372,200 (GRCm39)	V223A	probably damaging	Het
Sema4c	C	A	1: 36,589,774 (GRCm39)	V527L	probably benign	Het
Slk	C	A	19: 47,627,417 (GRCm39)	P1182T	probably damaging	Het
Stt3a	A	G	9: 36,659,235 (GRCm39)	V368A	probably benign	Het
Trpm3	T	C	19: 22,875,160 (GRCm39)	F601L	probably benign	Het
Ttn	T	C	2: 76,540,299 (GRCm39)	D34229G	probably benign	Het
Vmn2r107	A	G	17: 20,595,271 (GRCm39)	H608R	possibly damaging	Het
Zfp473	A	T	7: 44,387,368 (GRCm39)	F95Y	probably benign	Het
Zfp984	A	C	4: 147,839,287 (GRCm39)	S521R	possibly damaging	Het

Other mutations in Vmn2r49

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01800:Vmn2r49	APN	7	9,710,601 (GRCm39)	nonsense	probably null
IGL01892:Vmn2r49	APN	7	9,718,690 (GRCm39)	missense	probably benign	0.00
IGL02505:Vmn2r49	APN	7	9,710,378 (GRCm39)	missense	probably benign	0.27
IGL03345:Vmn2r49	APN	7	9,718,621 (GRCm39)	missense	probably damaging	1.00
PIT4434001:Vmn2r49	UTSW	7	9,710,762 (GRCm39)	missense	probably damaging	1.00
PIT4520001:Vmn2r49	UTSW	7	9,722,988 (GRCm39)	missense	probably benign	0.06
R0367:Vmn2r49	UTSW	7	9,710,357 (GRCm39)	missense	probably damaging	1.00
R0609:Vmn2r49	UTSW	7	9,710,233 (GRCm39)	missense	probably benign	0.40
R0931:Vmn2r49	UTSW	7	9,720,325 (GRCm39)	missense	possibly damaging	0.55
R1594:Vmn2r49	UTSW	7	9,710,550 (GRCm39)	missense	probably damaging	1.00
R1969:Vmn2r49	UTSW	7	9,720,235 (GRCm39)	missense	probably damaging	0.99
R4831:Vmn2r49	UTSW	7	9,720,352 (GRCm39)	missense	probably benign
R4944:Vmn2r49	UTSW	7	9,722,959 (GRCm39)	missense	probably benign
R4945:Vmn2r49	UTSW	7	9,720,214 (GRCm39)	nonsense	probably null
R5109:Vmn2r49	UTSW	7	9,710,204 (GRCm39)	missense	probably benign	0.01
R5141:Vmn2r49	UTSW	7	9,720,300 (GRCm39)	missense	probably benign	0.01
R5778:Vmn2r49	UTSW	7	9,710,274 (GRCm39)	missense	probably damaging	1.00
R6168:Vmn2r49	UTSW	7	9,718,713 (GRCm39)	missense	probably benign	0.01
R6513:Vmn2r49	UTSW	7	9,710,524 (GRCm39)	missense	probably damaging	0.99
R7347:Vmn2r49	UTSW	7	9,720,741 (GRCm39)	missense	probably benign	0.00
R7464:Vmn2r49	UTSW	7	9,722,820 (GRCm39)	missense	probably benign	0.03
R7750:Vmn2r49	UTSW	7	9,710,185 (GRCm39)	missense	probably damaging	1.00
R8031:Vmn2r49	UTSW	7	9,720,408 (GRCm39)	missense	possibly damaging	0.67
R9158:Vmn2r49	UTSW	7	9,722,835 (GRCm39)	missense	probably damaging	1.00
R9347:Vmn2r49	UTSW	7	9,718,674 (GRCm39)	missense	probably benign	0.00
R9553:Vmn2r49	UTSW	7	9,720,849 (GRCm39)	missense	probably benign	0.41

Predicted Primers

PCR Primer
(F):5'- ACAGTTCACATTGGAGTAGGG -3'
(R):5'- TCCTAAGCTATGAAGACCCCTTG -3'

Sequencing Primer
(F):5'- GTGTCCCTGATACAAGGAAGTATCTC -3'
(R):5'- CCTTGGGGATGGCACTTGC -3'

Posted On

2019-10-17