Mutagenetix > Incidental Mutation

Incidental Mutation 'R7497:Zfp160'

581169

Institutional Source

Beutler Lab

Gene Symbol

Zfp160

Ensembl Gene

ENSMUSG00000067942

Gene Name

zinc finger protein 160

Synonyms

6720480D16Rik, 6720480D16Rik

MMRRC Submission

045570-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7497 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21229203-21249119 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 21246455 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Lysine at position 335 (I335K)

Ref Sequence

ENSEMBL: ENSMUSP00000086191 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088811] [ENSMUST00000231482] [ENSMUST00000232320] [ENSMUST00000232354] [ENSMUST00000232473] [ENSMUST00000232663]

AlphaFold

E9Q459

Predicted Effect

probably benign
Transcript: ENSMUST00000088811
AA Change: I335K

PolyPhen 2 Score 0.077 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000086191
Gene: ENSMUSG00000067942
AA Change: I335K

Domain	Start	End	E-Value	Type
KRAB	8	68	1.91e-29	SMART
low complexity region	100	110	N/A	INTRINSIC
ZnF_C2H2	146	168	1.69e-3	SMART
ZnF_C2H2	174	196	2.91e-2	SMART
ZnF_C2H2	202	224	1.4e-4	SMART
ZnF_C2H2	230	252	3.89e-3	SMART
ZnF_C2H2	258	280	1.72e-4	SMART
ZnF_C2H2	286	308	4.94e-5	SMART
ZnF_C2H2	314	336	2.12e-4	SMART
ZnF_C2H2	342	364	1.12e-3	SMART
ZnF_C2H2	370	392	1.2e-3	SMART
ZnF_C2H2	398	420	6.42e-4	SMART
ZnF_C2H2	426	448	9.08e-4	SMART
ZnF_C2H2	454	476	1.84e-4	SMART
ZnF_C2H2	482	504	1.5e-4	SMART
ZnF_C2H2	510	532	3.44e-4	SMART
ZnF_C2H2	538	560	1.12e-3	SMART
ZnF_C2H2	566	588	2.27e-4	SMART
ZnF_C2H2	594	616	1.04e-3	SMART
ZnF_C2H2	622	644	1.13e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000231482

Predicted Effect

probably benign
Transcript: ENSMUST00000232320

Predicted Effect

probably benign
Transcript: ENSMUST00000232354

Predicted Effect

probably benign
Transcript: ENSMUST00000232473

Predicted Effect

probably benign
Transcript: ENSMUST00000232663

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (70/70)

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcd2	T	A	15: 91,075,379 (GRCm39)	I145F	probably benign	Het
Acvr2b	T	A	9: 119,262,352 (GRCm39)	V455E	probably benign	Het
Adgrv1	A	T	13: 81,588,344 (GRCm39)	V4414E	possibly damaging	Het
Agap2	T	C	10: 126,926,834 (GRCm39)	V977A	probably damaging	Het
Aph1b	A	C	9: 66,701,401 (GRCm39)	S79A	probably damaging	Het
Atm	G	A	9: 53,423,191 (GRCm39)	S645L	probably benign	Het
Cdh11	T	C	8: 103,400,456 (GRCm39)	R171G	probably benign	Het
Ces2f	T	A	8: 105,681,330 (GRCm39)	D556E	probably benign	Het
Cfap210	A	C	2: 69,588,792 (GRCm39)	N439K	probably benign	Het
Cyp26b1	C	A	6: 84,553,964 (GRCm39)	V218L	possibly damaging	Het
Diaph1	T	C	18: 38,028,353 (GRCm39)		probably null	Het
Dock1	A	G	7: 134,367,003 (GRCm39)	I482V	probably benign	Het
Dok4	A	T	8: 95,594,053 (GRCm39)	D47E	possibly damaging	Het
Dqx1	T	A	6: 83,036,028 (GRCm39)	L120Q	probably damaging	Het
Duxf3	A	T	10: 58,066,558 (GRCm39)	V157E	probably damaging	Het
Efcab3	T	C	11: 104,653,516 (GRCm39)		probably null	Het
Eif2a	C	A	3: 58,456,102 (GRCm39)	P367Q	probably damaging	Het
Elp2	C	T	18: 24,744,985 (GRCm39)	R102C	probably damaging	Het
Erich2	T	C	2: 70,364,666 (GRCm39)	S347P	probably damaging	Het
Fgfr3	GGACCTCTCCGTG	GG	5: 33,892,766 (GRCm39)		probably null	Het
Flacc1	T	A	1: 58,717,467 (GRCm39)	D148V	probably damaging	Het
Gadl1	A	T	9: 115,903,155 (GRCm39)	I495L	probably benign	Het
Gcnt4	A	G	13: 97,083,468 (GRCm39)	T255A	possibly damaging	Het
Gm10972	A	G	3: 94,550,887 (GRCm39)	K21E	unknown	Het
Gm57859	T	A	11: 113,583,223 (GRCm39)	W517R	probably damaging	Het
Gm7361	C	A	5: 26,466,188 (GRCm39)	H183Q	probably benign	Het
Gp2	C	T	7: 119,053,829 (GRCm39)	C44Y	probably damaging	Het
Hcar2	C	T	5: 124,003,249 (GRCm39)	V85I	probably benign	Het
Hira	T	C	16: 18,770,829 (GRCm39)	V822A	probably damaging	Het
Ighv1-5	T	A	12: 114,477,156 (GRCm39)	T49S	probably damaging	Het
Ints1	C	T	5: 139,754,731 (GRCm39)	V603M	probably damaging	Het
Kdm2a	A	C	19: 4,374,404 (GRCm39)	L909R	probably damaging	Het
Klkb1	T	A	8: 45,747,827 (GRCm39)		probably benign	Het
Krit1	A	G	5: 3,862,349 (GRCm39)	H168R	possibly damaging	Het
Map3k21	A	T	8: 126,654,340 (GRCm39)	E386D	probably damaging	Het
Muc16	C	T	9: 18,556,385 (GRCm39)	E3303K	unknown	Het
Muc5b	T	G	7: 141,415,250 (GRCm39)	V2732G	possibly damaging	Het
Myo5a	T	C	9: 75,104,983 (GRCm39)	L189P		Het
Nlrc5	T	C	8: 95,248,598 (GRCm39)	L1740S	probably damaging	Het
Nolc1	A	G	19: 46,071,257 (GRCm39)	K402R	probably benign	Het
Or4b1b	T	A	2: 90,112,098 (GRCm39)	T274S	possibly damaging	Het
Or4k15	T	A	14: 50,364,952 (GRCm39)	L306Q	probably benign	Het
Or52s19	T	C	7: 103,008,219 (GRCm39)	M61V	probably damaging	Het
Or8u8	T	C	2: 86,012,417 (GRCm39)	I13V	probably benign	Het
Pnma8b	A	G	7: 16,678,874 (GRCm39)		probably benign	Het
Pnpt1	A	T	11: 29,080,860 (GRCm39)	M35L	probably benign	Het
Postn	A	G	3: 54,270,091 (GRCm39)	K57E	probably damaging	Het
Ppp1r9a	A	C	6: 4,905,775 (GRCm39)	D110A	probably damaging	Het
Pptc7	G	A	5: 122,422,942 (GRCm39)	V71M	possibly damaging	Het
Prdm11	T	A	2: 92,843,052 (GRCm39)	I136F	possibly damaging	Het
Rfc1	A	G	5: 65,436,841 (GRCm39)	L613P	probably damaging	Het
Ryr3	T	C	2: 112,560,818 (GRCm39)	D2981G	probably benign	Het
Sbf2	C	A	7: 110,213,923 (GRCm39)	E16*	probably null	Het
Scara3	T	C	14: 66,168,651 (GRCm39)	E322G	probably damaging	Het
Sema5b	T	A	16: 35,481,700 (GRCm39)	C893S	probably damaging	Het
Setdb1	T	C	3: 95,249,139 (GRCm39)	D323G	probably damaging	Het
Slc19a3	T	C	1: 82,991,649 (GRCm39)	Y453C	probably damaging	Het
Snx5	T	C	2: 144,099,894 (GRCm39)	K137E	probably damaging	Het
Taar8c	G	A	10: 23,977,116 (GRCm39)	T232I	probably benign	Het
Taok2	G	A	7: 126,474,050 (GRCm39)	T352I	probably damaging	Het
Ttc3	C	A	16: 94,219,541 (GRCm39)	R489S	possibly damaging	Het
Usp12	A	T	5: 146,689,264 (GRCm39)		probably null	Het
Usp16	T	A	16: 87,263,174 (GRCm39)	C125*	probably null	Het
Vmn2r106	C	T	17: 20,488,201 (GRCm39)	E733K	probably damaging	Het
Vps13b	T	A	15: 35,876,843 (GRCm39)	I2832K	probably benign	Het
Vps13c	A	G	9: 67,747,761 (GRCm39)	Y18C	probably damaging	Het
Zfp788	A	T	7: 41,298,275 (GRCm39)	I304F	possibly damaging	Het

Other mutations in Zfp160

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00714:Zfp160	APN	17	21,246,964 (GRCm39)	missense	probably benign
IGL01019:Zfp160	APN	17	21,241,088 (GRCm39)	missense	possibly damaging	0.68
IGL02430:Zfp160	APN	17	21,245,792 (GRCm39)	missense	possibly damaging	0.76
R0412:Zfp160	UTSW	17	21,247,139 (GRCm39)	missense	probably damaging	0.97
R0600:Zfp160	UTSW	17	21,247,268 (GRCm39)	missense	probably benign	0.00
R2146:Zfp160	UTSW	17	21,247,244 (GRCm39)	missense	probably benign	0.13
R2157:Zfp160	UTSW	17	21,241,090 (GRCm39)	missense	probably benign	0.23
R2411:Zfp160	UTSW	17	21,246,007 (GRCm39)	missense	possibly damaging	0.94
R2904:Zfp160	UTSW	17	21,245,911 (GRCm39)	missense	probably benign	0.00
R4249:Zfp160	UTSW	17	21,246,000 (GRCm39)	missense	probably benign	0.11
R4896:Zfp160	UTSW	17	21,240,343 (GRCm39)	missense	probably benign	0.00
R5106:Zfp160	UTSW	17	21,247,023 (GRCm39)	missense	probably damaging	0.99
R5342:Zfp160	UTSW	17	21,240,995 (GRCm39)	missense	possibly damaging	0.95
R5352:Zfp160	UTSW	17	21,247,114 (GRCm39)	missense	probably benign	0.02
R6193:Zfp160	UTSW	17	21,247,124 (GRCm39)	missense	probably benign	0.24
R6230:Zfp160	UTSW	17	21,246,707 (GRCm39)	missense	probably benign	0.38
R6753:Zfp160	UTSW	17	21,240,996 (GRCm39)	missense	probably benign	0.02
R6928:Zfp160	UTSW	17	21,261,724 (GRCm39)	missense	probably benign	0.04
R7040:Zfp160	UTSW	17	21,246,794 (GRCm39)	missense	probably damaging	1.00
R7255:Zfp160	UTSW	17	21,245,749 (GRCm39)	missense	probably benign	0.18
R7510:Zfp160	UTSW	17	21,246,655 (GRCm39)	missense	probably benign	0.00
R7540:Zfp160	UTSW	17	21,245,922 (GRCm39)	nonsense	probably null
R7627:Zfp160	UTSW	17	21,247,270 (GRCm39)	missense	probably damaging	0.99
R8169:Zfp160	UTSW	17	21,247,298 (GRCm39)	missense	probably damaging	0.97
R8240:Zfp160	UTSW	17	21,246,350 (GRCm39)	missense	probably damaging	0.99
R8330:Zfp160	UTSW	17	21,246,313 (GRCm39)	missense	probably damaging	1.00
R8367:Zfp160	UTSW	17	21,245,804 (GRCm39)	missense	probably benign	0.22
R8802:Zfp160	UTSW	17	21,246,867 (GRCm39)	missense	probably damaging	1.00
R9183:Zfp160	UTSW	17	21,240,354 (GRCm39)	missense	possibly damaging	0.84
R9556:Zfp160	UTSW	17	21,247,031 (GRCm39)	missense	probably benign	0.03
R9695:Zfp160	UTSW	17	21,245,746 (GRCm39)	missense	possibly damaging	0.53
Z1177:Zfp160	UTSW	17	21,247,152 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGCGAAACCTTACAAATGTAATGAA -3'
(R):5'- AAGGCTTTTCCCCACTGTGA -3'

Sequencing Primer
(F):5'- GGTGTTCAGTCAGAATTCACACC -3'
(R):5'- TGAACTCCACGGTGACTAGCTAG -3'

Posted On

2019-10-17