Mutagenetix > Incidental Mutation

Incidental Mutation 'R7501:Cdc25b'

581454

Institutional Source

Beutler Lab

Gene Symbol

Cdc25b

Ensembl Gene

ENSMUSG00000027330

Gene Name

cell division cycle 25B

Synonyms

MMRRC Submission

045574-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7501 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

131028869-131040417 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 131036080 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 410 (Y410H)

Ref Sequence

ENSEMBL: ENSMUSP00000028804 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028804] [ENSMUST00000079857]

AlphaFold

P30306

Predicted Effect

probably damaging
Transcript: ENSMUST00000028804
AA Change: Y410H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000028804
Gene: ENSMUSG00000027330
AA Change: Y410H

Domain	Start	End	E-Value	Type
low complexity region	86	105	N/A	INTRINSIC
Pfam:M-inducer_phosp	111	379	3.3e-103	PFAM
RHOD	417	531	4.29e-26	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000079857
AA Change: Y384H

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000078784
Gene: ENSMUSG00000027330
AA Change: Y384H

Domain	Start	End	E-Value	Type
low complexity region	86	105	N/A	INTRINSIC
Pfam:M-inducer_phosp	111	354	2.4e-78	PFAM
RHOD	391	505	4.29e-26	SMART

Meta Mutation Damage Score

0.7318

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (61/61)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008]
PHENOTYPE: The resumption of meiosis during oocyte maturation is blocked in homozygous mutant female mice, resulting in female infertility. Male mice do not show an overt reproductive phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acad9	A	G	3: 36,142,974 (GRCm39)	N503S	probably benign	Het
Adam1a	C	T	5: 121,657,011 (GRCm39)	A761T	possibly damaging	Het
Amn1	C	T	6: 149,086,529 (GRCm39)	M44I	probably benign	Het
Ankrd44	T	C	1: 54,688,522 (GRCm39)	E238G		Het
Ap3b2	A	T	7: 81,123,194 (GRCm39)	I440K	probably damaging	Het
Atp2a1	G	A	7: 126,049,344 (GRCm39)	T566I	probably benign	Het
B3galnt1	T	C	3: 69,482,632 (GRCm39)	I210V	probably benign	Het
Bag5	T	C	12: 111,676,722 (GRCm39)	K367R	probably benign	Het
Cgref1	G	T	5: 31,102,800 (GRCm39)	P7Q	probably damaging	Het
Cnot6	T	C	11: 49,576,159 (GRCm39)	I136V	probably benign	Het
Comp	A	G	8: 70,832,059 (GRCm39)	D500G	possibly damaging	Het
Dnah7b	T	C	1: 46,395,714 (GRCm39)	L3872P	probably damaging	Het
Dync2h1	A	T	9: 7,175,336 (GRCm39)	L91Q	possibly damaging	Het
Fancd2	T	C	6: 113,525,364 (GRCm39)	V280A	possibly damaging	Het
Fat4	T	A	3: 39,012,597 (GRCm39)	Y2297*	probably null	Het
Fem1c	A	T	18: 46,638,868 (GRCm39)	M378K	probably damaging	Het
Gata6	A	G	18: 11,054,082 (GRCm39)	T4A	probably damaging	Het
Gatb	G	C	3: 85,544,297 (GRCm39)	V422L	probably damaging	Het
Gfpt1	T	A	6: 87,059,508 (GRCm39)	D510E	probably benign	Het
Gria4	A	T	9: 4,502,436 (GRCm39)	Y366N	probably benign	Het
Gucy1b1	T	C	3: 81,942,666 (GRCm39)	H492R	probably damaging	Het
H2bc6	A	G	13: 23,769,776 (GRCm39)	I55T	possibly damaging	Het
Hecw2	T	C	1: 53,953,031 (GRCm39)		probably null	Het
Hnrnph1	A	C	11: 50,270,383 (GRCm39)	E62D	probably benign	Het
Itga2	A	G	13: 115,012,095 (GRCm39)	V274A	probably damaging	Het
Kdm5d	T	A	Y: 941,488 (GRCm39)	W1230R	probably damaging	Het
Lctl	A	T	9: 64,038,861 (GRCm39)	M317L	probably benign	Het
Lrp6	T	C	6: 134,463,471 (GRCm39)	D570G	probably damaging	Het
Lrrc9	G	T	12: 72,496,490 (GRCm39)	M39I	probably damaging	Het
Ltbp2	A	T	12: 84,877,419 (GRCm39)	I402N	probably damaging	Het
Mst1	G	A	9: 107,959,748 (GRCm39)	G297D	probably damaging	Het
Muc6	G	C	7: 141,217,659 (GRCm39)	P2338R	probably damaging	Het
Nbr1	G	A	11: 101,457,026 (GRCm39)	R163Q	probably damaging	Het
Nlrp4f	A	G	13: 65,342,143 (GRCm39)	F501L	probably damaging	Het
Nup133	A	C	8: 124,649,153 (GRCm39)	I563S	probably benign	Het
Oacyl	T	G	18: 65,858,369 (GRCm39)		probably null	Het
Oog4	CAA	CA	4: 143,164,022 (GRCm39)		probably null	Het
Plxna2	C	T	1: 194,326,203 (GRCm39)	R46C	possibly damaging	Het
Ppp1r26	T	A	2: 28,340,749 (GRCm39)	D126E	probably damaging	Het
Prl6a1	A	T	13: 27,500,282 (GRCm39)	R84S	possibly damaging	Het
Ptprz1	C	T	6: 23,001,746 (GRCm39)	Q1279*	probably null	Het
Ptx4	C	T	17: 25,344,166 (GRCm39)	T472I	possibly damaging	Het
Rabgap1l	A	G	1: 160,528,358 (GRCm39)	V416A	probably damaging	Het
Rbfox2	A	T	15: 76,989,834 (GRCm39)	D231E	probably benign	Het
Reln	A	T	5: 22,432,636 (GRCm39)	F121L	possibly damaging	Het
Rfx7	G	A	9: 72,524,054 (GRCm39)	V415I	probably benign	Het
Scaf4	A	T	16: 90,026,964 (GRCm39)	M951K	unknown	Het
Sdf4	T	C	4: 156,080,977 (GRCm39)		probably null	Het
Sec16a	T	C	2: 26,331,863 (GRCm39)	T51A	probably damaging	Het
Sema3a	A	G	5: 13,607,008 (GRCm39)	N281S	probably damaging	Het
Snai2	G	A	16: 14,524,754 (GRCm39)	V87I	possibly damaging	Het
Spata31f1e	T	C	4: 42,791,357 (GRCm39)	E925G	probably damaging	Het
Synj2	T	A	17: 6,040,514 (GRCm39)	S197T	possibly damaging	Het
Syt6	A	T	3: 103,495,018 (GRCm39)	M328L	probably benign	Het
Tdrd12	A	G	7: 35,177,516 (GRCm39)	V946A	unknown	Het
Trat1	A	T	16: 48,574,657 (GRCm39)		probably null	Het
Vmn1r75	T	A	7: 11,614,997 (GRCm39)	I243K	possibly damaging	Het
Vmn2r83	G	A	10: 79,327,771 (GRCm39)	C793Y	probably damaging	Het
Wnt9a	G	A	11: 59,219,583 (GRCm39)	G203D	probably damaging	Het
Xrn2	T	C	2: 146,871,676 (GRCm39)	I366T	probably damaging	Het
Zfp280d	A	G	9: 72,269,224 (GRCm39)	D787G	possibly damaging	Het
Zfp706	T	C	15: 37,002,169 (GRCm39)	T53A	probably damaging	Het

Other mutations in Cdc25b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03230:Cdc25b	APN	2	131,030,060 (GRCm39)	missense	probably benign	0.00
R0471:Cdc25b	UTSW	2	131,039,204 (GRCm39)	missense	probably damaging	0.99
R0639:Cdc25b	UTSW	2	131,039,182 (GRCm39)	missense	probably benign	0.00
R0645:Cdc25b	UTSW	2	131,033,533 (GRCm39)	missense	probably benign	0.06
R0673:Cdc25b	UTSW	2	131,039,182 (GRCm39)	missense	probably benign	0.00
R1574:Cdc25b	UTSW	2	131,033,057 (GRCm39)	splice site	probably benign
R4094:Cdc25b	UTSW	2	131,031,037 (GRCm39)	missense	probably benign
R4433:Cdc25b	UTSW	2	131,033,618 (GRCm39)	missense	probably benign	0.02
R4722:Cdc25b	UTSW	2	131,035,271 (GRCm39)	missense	probably damaging	1.00
R4817:Cdc25b	UTSW	2	131,035,223 (GRCm39)	missense	probably damaging	1.00
R4957:Cdc25b	UTSW	2	131,035,525 (GRCm39)	missense	possibly damaging	0.80
R5345:Cdc25b	UTSW	2	131,034,516 (GRCm39)	missense	probably benign	0.18
R5407:Cdc25b	UTSW	2	131,035,567 (GRCm39)	missense	probably damaging	1.00
R5562:Cdc25b	UTSW	2	131,036,678 (GRCm39)	missense	probably damaging	1.00
R5594:Cdc25b	UTSW	2	131,033,538 (GRCm39)	missense	probably damaging	1.00
R5792:Cdc25b	UTSW	2	131,033,679 (GRCm39)	missense	probably damaging	1.00
R5831:Cdc25b	UTSW	2	131,029,301 (GRCm39)	critical splice donor site	probably null
R7204:Cdc25b	UTSW	2	131,033,552 (GRCm39)	missense	probably damaging	1.00
R7292:Cdc25b	UTSW	2	131,033,093 (GRCm39)	missense	probably damaging	1.00
R7399:Cdc25b	UTSW	2	131,036,574 (GRCm39)	missense	probably damaging	1.00
R7772:Cdc25b	UTSW	2	131,031,029 (GRCm39)	missense	probably damaging	1.00
R8186:Cdc25b	UTSW	2	131,031,050 (GRCm39)	missense	probably benign	0.05
R8783:Cdc25b	UTSW	2	131,033,772 (GRCm39)	missense	probably benign	0.05
R8985:Cdc25b	UTSW	2	131,035,180 (GRCm39)	missense	probably damaging	1.00
R9141:Cdc25b	UTSW	2	131,033,857 (GRCm39)	critical splice donor site	probably null
R9153:Cdc25b	UTSW	2	131,034,564 (GRCm39)	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- GCAGAAGGTTCTTTCCCTGG -3'
(R):5'- GCTGAACTTGCCTGTTAACAG -3'

Sequencing Primer
(F):5'- CAGAAGGTTCTTTCCCTGGGTATG -3'
(R):5'- TGTTAACAGGGCCACCATCTGTAG -3'

Posted On

2019-10-17