Mutagenetix > Incidental Mutation

Incidental Mutation 'R7508:Vmn2r32'

581893

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r32

Ensembl Gene

ENSMUSG00000096743

Gene Name

vomeronasal 2, receptor 32

Synonyms

V2r5

MMRRC Submission

045581-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.190) question?

Stock #

R7508 (G1)

Quality Score

121.008

Status

Not validated

Chromosome

Chromosomal Location

7463015-7479973 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 7467374 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 515 (V515M)

Ref Sequence

ENSEMBL: ENSMUSP00000092462 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094866]

AlphaFold

K7N686

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094866
AA Change: V515M

PolyPhen 2 Score 0.870 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000092462
Gene: ENSMUSG00000096743
AA Change: V515M

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	3.6e-33	PFAM
Pfam:NCD3G	512	565	8.1e-21	PFAM
Pfam:7tm_3	598	833	9.5e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (56/56)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgra3	C	T	5: 50,016,867 (GRCm38)	A110T	probably benign	Het
Adra1a	A	C	14: 66,637,935 (GRCm38)	I120L	probably damaging	Het
Ahnak2	C	T	12: 112,774,405 (GRCm38)	V1078I	possibly damaging	Het
Ccser1	A	G	6: 61,570,723 (GRCm38)	H590R	probably benign	Het
Cdh12	T	G	15: 21,583,765 (GRCm38)	L564V	probably benign	Het
Celsr3	T	A	9: 108,836,622 (GRCm38)	H1900Q	probably benign	Het
Clasp1	T	A	1: 118,545,434 (GRCm38)	M918K	probably benign	Het
Cmtm6	A	G	9: 114,731,240 (GRCm38)	E2G	probably damaging	Het
Ctsg	A	G	14: 56,100,541 (GRCm38)		probably null	Het
Cylc2	T	A	4: 51,229,256 (GRCm38)		probably null	Het
Dna2	A	G	10: 62,971,993 (GRCm38)		probably null	Het
Dnaic1	G	A	4: 41,614,323 (GRCm38)	R333H	probably benign	Het
Egln3	T	C	12: 54,180,628 (GRCm38)	D239G	probably benign	Het
Fam13a	A	T	6: 58,987,284 (GRCm38)	D54E	probably damaging	Het
Fn1	A	G	1: 71,597,516 (GRCm38)	V2159A	probably benign	Het
Gcnt2	T	A	13: 40,887,681 (GRCm38)	F105L	probably benign	Het
Gm5788	T	C	12: 87,494,842 (GRCm38)	M41T	possibly damaging	Het
Gpd1	G	A	15: 99,722,086 (GRCm38)	S255N	probably damaging	Het
Hacd4	A	G	4: 88,437,478 (GRCm38)	F57L	probably benign	Het
Helb	T	C	10: 120,105,283 (GRCm38)	D500G	probably benign	Het
Ighv1-26	G	A	12: 114,788,442 (GRCm38)	S94F	probably damaging	Het
Kirrel	T	C	3: 87,083,439 (GRCm38)	D692G	possibly damaging	Het
Klra7	A	T	6: 130,230,091 (GRCm38)		probably null	Het
Lyl1	T	C	8: 84,704,300 (GRCm38)	V277A	probably benign	Het
Mon2	A	C	10: 123,023,939 (GRCm38)	W811G	probably damaging	Het
Myo9b	T	C	8: 71,354,801 (GRCm38)	L1627P	probably benign	Het
Neurl1b	C	G	17: 26,438,746 (GRCm38)	H219Q	probably benign	Het
Odf4	A	T	11: 68,922,423 (GRCm38)	C218S	possibly damaging	Het
Olfr1038-ps	A	G	2: 86,121,938 (GRCm38)	N5S	possibly damaging	Het
Olfr11	T	A	13: 21,638,609 (GRCm38)	I305F	probably benign	Het
Pde5a	A	G	3: 122,818,030 (GRCm38)	D571G	probably damaging	Het
Pgc	A	G	17: 47,734,186 (GRCm38)	E343G	probably benign	Het
Pik3cd	A	G	4: 149,654,583 (GRCm38)	F667L	possibly damaging	Het
Prf1	G	A	10: 61,300,155 (GRCm38)	R70H	possibly damaging	Het
Ptprb	C	T	10: 116,353,991 (GRCm38)	Q1565*	probably null	Het
Rapgef4	T	A	2: 72,205,733 (GRCm38)	N523K	probably benign	Het
Rbm25	T	C	12: 83,672,877 (GRCm38)	L557P	probably damaging	Het
Rhobtb3	C	T	13: 75,878,857 (GRCm38)	V466I	probably benign	Het
Rnpep	A	G	1: 135,278,858 (GRCm38)	V166A	probably benign	Het
Sgo2a	A	G	1: 58,017,795 (GRCm38)	K1046R	probably benign	Het
Slc12a2	T	C	18: 57,904,393 (GRCm38)	V525A	probably benign	Het
Slc1a4	A	T	11: 20,306,487 (GRCm38)	I448N	probably damaging	Het
Slc29a4	C	T	5: 142,718,506 (GRCm38)	P305L	probably benign	Het
Slc9a5	T	A	8: 105,363,253 (GRCm38)		probably null	Het
Spag16	A	T	1: 69,887,520 (GRCm38)	N258I	possibly damaging	Het
Sspo	T	A	6: 48,466,699 (GRCm38)	L2076Q	probably damaging	Het
Taok1	T	A	11: 77,545,326 (GRCm38)	H704L	probably damaging	Het
Tbc1d9b	T	C	11: 50,145,120 (GRCm38)	F148L	probably damaging	Het
Tm4sf1	A	G	3: 57,294,755 (GRCm38)	Y12H	probably benign	Het
Traj6	C	T	14: 54,212,714 (GRCm38)	T9M		Het
Ttll4	A	G	1: 74,687,259 (GRCm38)	N672S	possibly damaging	Het
Ube3a	T	A	7: 59,303,689 (GRCm38)	H790Q	possibly damaging	Het
Usp49	G	A	17: 47,672,280 (GRCm38)	R70Q	probably benign	Het
Wars	A	G	12: 108,882,875 (GRCm38)	S49P	probably benign	Het
Zbp1	T	A	2: 173,207,811 (GRCm38)	Q386L	possibly damaging	Het
Zfp369	T	C	13: 65,279,273 (GRCm38)	F10S	unknown	Het
Zfp512	C	G	5: 31,473,539 (GRCm38)	I408M	possibly damaging	Het
Zfp952	A	G	17: 33,003,782 (GRCm38)	I412V	probably benign	Het

Other mutations in Vmn2r32

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02140:Vmn2r32	APN	7	7,476,697 (GRCm38)	missense	probably damaging	0.97
IGL02333:Vmn2r32	APN	7	7,464,144 (GRCm38)	missense	probably damaging	1.00
IGL02406:Vmn2r32	APN	7	7,476,710 (GRCm38)	missense	probably benign
IGL02428:Vmn2r32	APN	7	7,474,284 (GRCm38)	missense	probably benign	0.02
IGL02484:Vmn2r32	APN	7	7,464,117 (GRCm38)	missense	probably damaging	1.00
IGL03277:Vmn2r32	APN	7	7,474,252 (GRCm38)	missense	probably benign	0.23
IGL03366:Vmn2r32	APN	7	7,464,030 (GRCm38)	missense	probably damaging	0.99
R1055:Vmn2r32	UTSW	7	7,474,327 (GRCm38)	nonsense	probably null
R1695:Vmn2r32	UTSW	7	7,463,992 (GRCm38)	missense	probably benign	0.01
R2172:Vmn2r32	UTSW	7	7,474,615 (GRCm38)	missense	probably damaging	0.99
R2262:Vmn2r32	UTSW	7	7,474,619 (GRCm38)	missense	probably benign
R3150:Vmn2r32	UTSW	7	7,472,555 (GRCm38)	missense	probably benign
R4362:Vmn2r32	UTSW	7	7,479,858 (GRCm38)	nonsense	probably null
R4432:Vmn2r32	UTSW	7	7,479,919 (GRCm38)	missense	probably damaging	0.98
R4851:Vmn2r32	UTSW	7	7,479,954 (GRCm38)	missense	possibly damaging	0.59
R4949:Vmn2r32	UTSW	7	7,464,084 (GRCm38)	missense	probably benign	0.22
R5990:Vmn2r32	UTSW	7	7,479,810 (GRCm38)	missense	probably damaging	0.97
R6083:Vmn2r32	UTSW	7	7,464,210 (GRCm38)	missense	probably benign	0.15
R6084:Vmn2r32	UTSW	7	7,464,210 (GRCm38)	missense	probably benign	0.15
R6116:Vmn2r32	UTSW	7	7,464,093 (GRCm38)	missense	probably damaging	1.00
R6263:Vmn2r32	UTSW	7	7,476,692 (GRCm38)	missense	possibly damaging	0.90
R6889:Vmn2r32	UTSW	7	7,472,574 (GRCm38)	missense	possibly damaging	0.82
R7286:Vmn2r32	UTSW	7	7,479,808 (GRCm38)	missense	probably benign	0.21
R7390:Vmn2r32	UTSW	7	7,479,852 (GRCm38)	missense	probably benign	0.00
R7412:Vmn2r32	UTSW	7	7,474,213 (GRCm38)	missense	possibly damaging	0.53
R8812:Vmn2r32	UTSW	7	7,474,670 (GRCm38)	missense	probably damaging	1.00
R8968:Vmn2r32	UTSW	7	7,474,205 (GRCm38)	missense	probably benign	0.00
R9331:Vmn2r32	UTSW	7	7,464,403 (GRCm38)	nonsense	probably null
R9358:Vmn2r32	UTSW	7	7,474,198 (GRCm38)	missense	probably damaging	1.00
Z1177:Vmn2r32	UTSW	7	7,474,161 (GRCm38)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TGACTTCATTATTCCAGGGTGATG -3'
(R):5'- ACCAGGTTGCAACTTCTGTAG -3'

Sequencing Primer
(F):5'- TGGAAGAGAAATACATATCTTCGGG -3'
(R):5'- TAATCCCAGCACTTTGGAGG -3'

Posted On

2019-10-17