Mutagenetix > Incidental Mutation

Incidental Mutation 'R0008:Ubr4'

58203

Institutional Source

Beutler Lab

Gene Symbol

Ubr4

Ensembl Gene

ENSMUSG00000066036

Gene Name

ubiquitin protein ligase E3 component n-recognin 4

Synonyms

p600, A930005E13Rik, LOC381562, D930005K06Rik, Zubr1, 1810009A16Rik

MMRRC Submission

038303-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0008 (G1)

Quality Score

140

Status

Validated

Chromosome

Chromosomal Location

139107970-139216844 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 139157487 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Methionine at position 2348 (T2348M)

Ref Sequence

ENSEMBL: ENSMUSP00000125800 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000097822] [ENSMUST00000129779] [ENSMUST00000147999] [ENSMUST00000165860]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000097822
AA Change: T2348M

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000095433
Gene: ENSMUSG00000066036
AA Change: T2348M

Domain	Start	End	E-Value	Type
low complexity region	5	20	N/A	INTRINSIC
low complexity region	414	425	N/A	INTRINSIC
low complexity region	530	541	N/A	INTRINSIC
low complexity region	555	566	N/A	INTRINSIC
low complexity region	571	588	N/A	INTRINSIC
low complexity region	600	621	N/A	INTRINSIC
low complexity region	1312	1330	N/A	INTRINSIC
low complexity region	1642	1655	N/A	INTRINSIC
Pfam:zf-UBR	1660	1725	1.9e-9	PFAM
Blast:ZnF_C2H2	1966	1991	6e-8	BLAST
low complexity region	2268	2279	N/A	INTRINSIC
low complexity region	2502	2540	N/A	INTRINSIC
low complexity region	2725	2735	N/A	INTRINSIC
low complexity region	2818	2852	N/A	INTRINSIC
low complexity region	2887	2905	N/A	INTRINSIC
low complexity region	2928	2942	N/A	INTRINSIC
low complexity region	2945	2959	N/A	INTRINSIC
low complexity region	2966	2986	N/A	INTRINSIC
low complexity region	3063	3091	N/A	INTRINSIC
low complexity region	3329	3385	N/A	INTRINSIC
low complexity region	3776	3788	N/A	INTRINSIC
Pfam:E3_UbLigase_R4	4364	5160	N/A	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000129779

Predicted Effect

possibly damaging
Transcript: ENSMUST00000129949
AA Change: T1059M

PolyPhen 2 Score 0.629 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000115711
Gene: ENSMUSG00000066036
AA Change: T1059M

Domain	Start	End	E-Value	Type
low complexity region	24	42	N/A	INTRINSIC
low complexity region	354	367	N/A	INTRINSIC
Pfam:zf-UBR	372	437	2.1e-10	PFAM
Blast:ZnF_C2H2	678	703	5e-8	BLAST
low complexity region	980	991	N/A	INTRINSIC
low complexity region	1225	1263	N/A	INTRINSIC
low complexity region	1448	1458	N/A	INTRINSIC
low complexity region	1575	1593	N/A	INTRINSIC
low complexity region	1616	1630	N/A	INTRINSIC
low complexity region	1633	1647	N/A	INTRINSIC
low complexity region	1654	1674	N/A	INTRINSIC
low complexity region	1751	1779	N/A	INTRINSIC
low complexity region	2017	2045	N/A	INTRINSIC
low complexity region	2048	2065	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000147999

SMART Domains

Protein: ENSMUSP00000117419
Gene: ENSMUSG00000066036

Domain	Start	End	E-Value	Type
low complexity region	170	226	N/A	INTRINSIC
low complexity region	617	629	N/A	INTRINSIC
Pfam:E3_UbLigase_R4	1205	1301	4.5e-60	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000165860
AA Change: T2348M

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000125800
Gene: ENSMUSG00000066036
AA Change: T2348M

Domain	Start	End	E-Value	Type
low complexity region	5	20	N/A	INTRINSIC
low complexity region	414	425	N/A	INTRINSIC
low complexity region	530	541	N/A	INTRINSIC
low complexity region	555	566	N/A	INTRINSIC
low complexity region	571	588	N/A	INTRINSIC
low complexity region	600	621	N/A	INTRINSIC
low complexity region	1312	1330	N/A	INTRINSIC
low complexity region	1642	1655	N/A	INTRINSIC
Pfam:zf-UBR	1659	1729	4e-13	PFAM
Blast:ZnF_C2H2	1966	1991	5e-8	BLAST
low complexity region	2268	2279	N/A	INTRINSIC
low complexity region	2513	2551	N/A	INTRINSIC
low complexity region	2736	2746	N/A	INTRINSIC
low complexity region	2863	2881	N/A	INTRINSIC
low complexity region	2904	2918	N/A	INTRINSIC
low complexity region	2921	2935	N/A	INTRINSIC
low complexity region	2942	2962	N/A	INTRINSIC
low complexity region	3039	3067	N/A	INTRINSIC
low complexity region	3305	3361	N/A	INTRINSIC
low complexity region	3752	3764	N/A	INTRINSIC
Pfam:E3_UbLigase_R4	4340	5136	N/A	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.4%
20x: 94.8%

Validation Efficiency

98% (109/111)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010]
PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit neonatal lethality and decreased body size at birth. Mice homozygous for a null mutation display complete embryonic lethality during organogenesis with arrest of vitelline vascular remodeling. [provided by MGI curators]

Allele List at MGI

All alleles(59) : Targeted(2) Gene trapped(56) Transgenic(1)

Other mutations in this stock

Total: 98 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930579F01Rik	T	C	3: 137,882,346 (GRCm39)	K118R	possibly damaging	Het
Adtrp	T	C	13: 41,920,941 (GRCm39)	T88A	probably damaging	Het
Afap1l1	A	G	18: 61,889,976 (GRCm39)	S87P	probably benign	Het
Ankrd27	A	G	7: 35,303,125 (GRCm39)	K196R	probably benign	Het
Apoe	G	A	7: 19,431,005 (GRCm39)	T79M	probably damaging	Het
Arrdc3	T	A	13: 81,032,011 (GRCm39)	Y81*	probably null	Het
Arrdc3	T	A	13: 81,039,194 (GRCm39)	I75N	probably damaging	Het
Asah2	T	A	19: 31,981,131 (GRCm39)	K629*	probably null	Het
Ass1	A	T	2: 31,404,831 (GRCm39)	N371Y	probably damaging	Het
C130074G19Rik	A	G	1: 184,615,119 (GRCm39)	S24P	probably benign	Het
C87436	A	G	6: 86,423,265 (GRCm39)		probably benign	Het
Calcrl	T	C	2: 84,203,618 (GRCm39)	D54G	probably benign	Het
Clcn2	T	C	16: 20,529,140 (GRCm39)	N367S	probably null	Het
Cnot1	G	T	8: 96,487,969 (GRCm39)	D562E	probably damaging	Het
Commd6	G	A	14: 101,877,709 (GRCm39)		probably benign	Het
Cox6a2	G	A	7: 127,805,212 (GRCm39)		probably benign	Het
Cp	T	A	3: 20,022,287 (GRCm39)	Y230N	probably damaging	Het
Dclre1c	T	C	2: 3,439,032 (GRCm39)	V64A	probably damaging	Het
Eng	T	C	2: 32,567,692 (GRCm39)	V110A	probably damaging	Het
Esyt3	T	C	9: 99,220,860 (GRCm39)	I114M	possibly damaging	Het
Fam83h	A	T	15: 75,875,811 (GRCm39)	Y509N	probably damaging	Het
Fat2	A	T	11: 55,202,075 (GRCm39)	L333H	probably damaging	Het
Fbxo21	A	G	5: 118,146,078 (GRCm39)	N567S	possibly damaging	Het
Fcsk	T	C	8: 111,610,865 (GRCm39)		probably benign	Het
Fn1	A	T	1: 71,634,879 (GRCm39)	L1964Q	probably damaging	Het
Gorasp1	G	T	9: 119,757,312 (GRCm39)	S353R	possibly damaging	Het
Grk2	C	T	19: 4,337,262 (GRCm39)	E646K	probably damaging	Het
Hoxc11	T	C	15: 102,863,397 (GRCm39)	V146A	probably damaging	Het
Igf2bp2	T	C	16: 21,894,841 (GRCm39)	T301A	probably benign	Het
Il11	T	C	7: 4,776,658 (GRCm39)	S111G	probably benign	Het
Ist1	A	T	8: 110,403,418 (GRCm39)	I273K	probably benign	Het
Kdm2b	G	A	5: 123,019,806 (GRCm39)	S738L	probably benign	Het
Lrp2	T	A	2: 69,346,895 (GRCm39)	N784Y	probably benign	Het
Lrp6	T	C	6: 134,462,716 (GRCm39)	E648G	probably damaging	Het
Mapk15	G	A	15: 75,870,103 (GRCm39)	E408K	probably benign	Het
Mdn1	T	A	4: 32,718,317 (GRCm39)	F2191I	possibly damaging	Het
Metrn	C	A	17: 26,015,479 (GRCm39)	V79F	possibly damaging	Het
Mtbp	T	A	15: 55,449,889 (GRCm39)		probably benign	Het
Myh11	C	A	16: 14,041,883 (GRCm39)	Q720H	probably damaging	Het
Myo3a	T	A	2: 22,469,753 (GRCm39)	I508N	probably damaging	Het
Nat9	A	T	11: 115,075,941 (GRCm39)	Y27N	probably damaging	Het
Ncapg2	T	C	12: 116,393,455 (GRCm39)	F553S	probably damaging	Het
Nipsnap3b	T	A	4: 53,015,112 (GRCm39)	L53Q	probably damaging	Het
Nlrp3	A	T	11: 59,449,274 (GRCm39)	H852L	probably benign	Het
Or2d3b	A	G	7: 106,514,226 (GRCm39)	I274V	probably benign	Het
Or2t6	T	A	14: 14,176,092 (GRCm38)		probably benign	Het
Or4a78	T	A	2: 89,497,428 (GRCm39)	K267N	probably damaging	Het
Or52e3	A	T	7: 102,869,558 (GRCm39)	D211V	probably damaging	Het
Or52e3	G	A	7: 102,869,584 (GRCm39)	A220T	probably benign	Het
Or5b122	A	G	19: 13,563,240 (GRCm39)	I191V	probably benign	Het
Pax9	A	G	12: 56,756,528 (GRCm39)	T289A	probably benign	Het
Pcyt2	A	T	11: 120,506,695 (GRCm39)	I53N	possibly damaging	Het
Pdlim4	C	T	11: 53,945,875 (GRCm39)	V327M	probably damaging	Het
Pdzph1	T	A	17: 59,229,756 (GRCm39)		probably benign	Het
Plekhm2	C	T	4: 141,369,704 (GRCm39)		probably benign	Het
Ppt1	T	C	4: 122,742,216 (GRCm39)		probably benign	Het
Prdm1	C	T	10: 44,317,675 (GRCm39)	E398K	probably damaging	Het
Prep	T	C	10: 44,991,174 (GRCm39)	V280A	probably benign	Het
Prkdc	T	A	16: 15,526,565 (GRCm39)		probably benign	Het
Proser3	G	A	7: 30,239,563 (GRCm39)	R514C	probably damaging	Het
Ptk7	G	A	17: 46,883,688 (GRCm39)		probably benign	Het
Rbm45	T	C	2: 76,208,742 (GRCm39)	Y293H	probably damaging	Het
Rnf213	A	C	11: 119,355,878 (GRCm39)	E4108A	possibly damaging	Het
Sdk2	A	G	11: 113,747,581 (GRCm39)	L643P	probably damaging	Het
Sec24d	C	A	3: 123,144,525 (GRCm39)		probably benign	Het
Sh2d3c	C	G	2: 32,643,033 (GRCm39)	H587D	probably damaging	Het
Slc1a1	G	A	19: 28,878,884 (GRCm39)	G208S	probably benign	Het
Slc35b4	A	T	6: 34,135,452 (GRCm39)	Y287N	probably damaging	Het
Slc46a2	T	A	4: 59,914,544 (GRCm39)	L126F	probably damaging	Het
Slc4a8	T	C	15: 100,698,374 (GRCm39)	M621T	possibly damaging	Het
Slc9b2	T	A	3: 135,042,269 (GRCm39)	V516D	possibly damaging	Het
Slco1a6	T	A	6: 142,102,948 (GRCm39)		probably benign	Het
Sncg	C	T	14: 34,096,495 (GRCm39)	V15I	probably benign	Het
Srgap2	T	C	1: 131,283,302 (GRCm39)	T260A	probably damaging	Het
Stk10	T	A	11: 32,537,305 (GRCm39)		probably benign	Het
Taf5	A	G	19: 47,064,301 (GRCm39)	S415G	possibly damaging	Het
Tdp1	C	T	12: 99,921,217 (GRCm39)		probably benign	Het
Tdp2	T	G	13: 25,025,333 (GRCm39)		probably null	Het
Tgfbi	T	A	13: 56,777,587 (GRCm39)	I357N	probably benign	Het
Tmem116	A	G	5: 121,633,159 (GRCm39)	T178A	probably damaging	Het
Tnrc6a	G	A	7: 122,769,617 (GRCm39)	R469H	probably benign	Het
Top2a	A	T	11: 98,893,729 (GRCm39)	L1055*	probably null	Het
Tox	T	A	4: 6,842,411 (GRCm39)	M40L	probably benign	Het
Trib2	A	T	12: 15,859,930 (GRCm39)	H110Q	probably benign	Het
Trpa1	A	G	1: 14,973,439 (GRCm39)	I293T	possibly damaging	Het
Trpv2	A	G	11: 62,481,086 (GRCm39)	Y395C	probably damaging	Het
Ubn2	T	A	6: 38,411,535 (GRCm39)		probably null	Het
Ubxn4	G	A	1: 128,190,641 (GRCm39)	E256K	probably benign	Het
Vmn1r33	C	A	6: 66,589,510 (GRCm39)	G15*	probably null	Het
Vmn1r37	T	A	6: 66,708,769 (GRCm39)	S95T	probably benign	Het
Vmn2r57	A	G	7: 41,050,076 (GRCm39)	C558R	probably damaging	Het
Vnn1	T	C	10: 23,774,500 (GRCm39)		probably null	Het
Vps13c	T	C	9: 67,826,544 (GRCm39)	V1395A	probably benign	Het
Vwa7	A	G	17: 35,238,781 (GRCm39)	I290V	probably benign	Het
Wdr93	A	G	7: 79,408,221 (GRCm39)	E234G	probably damaging	Het
Zfp385b	A	T	2: 77,246,291 (GRCm39)	S245R	probably benign	Het
Zfp942	A	T	17: 22,147,319 (GRCm39)	C437S	probably damaging	Het
Zfyve9	T	A	4: 108,575,902 (GRCm39)	E393V	possibly damaging	Het

Other mutations in Ubr4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00087:Ubr4	APN	4	139,192,633 (GRCm39)	missense	possibly damaging	0.46
IGL00336:Ubr4	APN	4	139,155,877 (GRCm39)	missense	probably damaging	1.00
IGL00586:Ubr4	APN	4	139,182,495 (GRCm39)	missense	possibly damaging	0.95
IGL00659:Ubr4	APN	4	139,148,556 (GRCm39)	missense	probably damaging	1.00
IGL00766:Ubr4	APN	4	139,168,077 (GRCm39)	missense	probably damaging	1.00
IGL00819:Ubr4	APN	4	139,203,593 (GRCm39)	missense	possibly damaging	0.92
IGL00833:Ubr4	APN	4	139,120,470 (GRCm39)	critical splice donor site	probably null
IGL01126:Ubr4	APN	4	139,129,866 (GRCm39)	missense	probably benign	0.04
IGL01311:Ubr4	APN	4	139,206,356 (GRCm39)	missense	possibly damaging	0.66
IGL01349:Ubr4	APN	4	139,208,039 (GRCm39)	missense	unknown
IGL01388:Ubr4	APN	4	139,187,554 (GRCm39)	missense	possibly damaging	0.76
IGL01417:Ubr4	APN	4	139,138,111 (GRCm39)	missense	probably damaging	0.99
IGL01446:Ubr4	APN	4	139,165,351 (GRCm39)	splice site	probably benign
IGL01449:Ubr4	APN	4	139,140,047 (GRCm39)	missense	probably damaging	0.98
IGL01545:Ubr4	APN	4	139,170,140 (GRCm39)	splice site	probably benign
IGL01568:Ubr4	APN	4	139,148,684 (GRCm39)	missense	probably damaging	1.00
IGL01596:Ubr4	APN	4	139,189,845 (GRCm39)	splice site	probably benign
IGL01621:Ubr4	APN	4	139,168,094 (GRCm39)	nonsense	probably null
IGL01639:Ubr4	APN	4	139,144,655 (GRCm39)	missense	probably damaging	0.99
IGL01655:Ubr4	APN	4	139,135,107 (GRCm39)	missense	probably damaging	1.00
IGL01710:Ubr4	APN	4	139,145,772 (GRCm39)	missense	possibly damaging	0.81
IGL01830:Ubr4	APN	4	139,199,811 (GRCm39)	missense	probably damaging	0.99
IGL01862:Ubr4	APN	4	139,204,469 (GRCm39)	missense	possibly damaging	0.66
IGL01868:Ubr4	APN	4	139,139,989 (GRCm39)	nonsense	probably null
IGL01874:Ubr4	APN	4	139,120,600 (GRCm39)	splice site	probably benign
IGL01889:Ubr4	APN	4	139,189,783 (GRCm39)	nonsense	probably null
IGL01891:Ubr4	APN	4	139,163,571 (GRCm39)	critical splice donor site	probably null
IGL01980:Ubr4	APN	4	139,156,913 (GRCm39)	missense	probably damaging	0.99
IGL02126:Ubr4	APN	4	139,180,052 (GRCm39)	critical splice donor site	probably null
IGL02153:Ubr4	APN	4	139,187,471 (GRCm39)	nonsense	probably null
IGL02173:Ubr4	APN	4	139,164,381 (GRCm39)	splice site	probably null
IGL02214:Ubr4	APN	4	139,155,894 (GRCm39)	missense	possibly damaging	0.95
IGL02214:Ubr4	APN	4	139,189,138 (GRCm39)	critical splice acceptor site	probably null
IGL02220:Ubr4	APN	4	139,115,746 (GRCm39)	missense	probably benign	0.01
IGL02246:Ubr4	APN	4	139,186,414 (GRCm39)	missense	possibly damaging	0.89
IGL02264:Ubr4	APN	4	139,182,939 (GRCm39)	nonsense	probably null
IGL02273:Ubr4	APN	4	139,199,889 (GRCm39)	missense	possibly damaging	0.94
IGL02316:Ubr4	APN	4	139,200,489 (GRCm39)	missense	possibly damaging	0.46
IGL02328:Ubr4	APN	4	139,206,233 (GRCm39)	missense	probably damaging	0.97
IGL02476:Ubr4	APN	4	139,148,560 (GRCm39)	nonsense	probably null
IGL02477:Ubr4	APN	4	139,163,516 (GRCm39)	missense	probably damaging	0.98
IGL02544:Ubr4	APN	4	139,142,429 (GRCm39)	missense	probably damaging	1.00
IGL02622:Ubr4	APN	4	139,194,561 (GRCm39)	nonsense	probably null
IGL02679:Ubr4	APN	4	139,186,445 (GRCm39)	missense	probably damaging	0.99
IGL02728:Ubr4	APN	4	139,196,122 (GRCm39)	missense	probably damaging	1.00
IGL02754:Ubr4	APN	4	139,138,095 (GRCm39)	missense	probably damaging	0.99
IGL02754:Ubr4	APN	4	139,120,470 (GRCm39)	critical splice donor site	probably null
IGL02892:Ubr4	APN	4	139,144,642 (GRCm39)	missense	probably damaging	0.96
IGL02897:Ubr4	APN	4	139,199,819 (GRCm39)	missense	probably damaging	0.97
IGL02946:Ubr4	APN	4	139,152,606 (GRCm39)	missense	probably damaging	1.00
IGL02964:Ubr4	APN	4	139,135,131 (GRCm39)	missense	possibly damaging	0.84
IGL03059:Ubr4	APN	4	139,207,987 (GRCm39)	missense	probably damaging	0.98
IGL03068:Ubr4	APN	4	139,137,041 (GRCm39)	missense	probably benign	0.02
IGL03087:Ubr4	APN	4	139,177,668 (GRCm39)	nonsense	probably null
IGL03116:Ubr4	APN	4	139,206,892 (GRCm39)	splice site	probably benign
IGL03212:Ubr4	APN	4	139,137,074 (GRCm39)	missense	probably benign	0.13
IGL03228:Ubr4	APN	4	139,156,909 (GRCm39)	missense	probably damaging	1.00
IGL03292:Ubr4	APN	4	139,167,746 (GRCm39)	missense	probably damaging	1.00
IGL03388:Ubr4	APN	4	139,142,343 (GRCm39)	missense	probably damaging	0.98
IGL03393:Ubr4	APN	4	139,179,989 (GRCm39)	missense	probably damaging	1.00
IGL03409:Ubr4	APN	4	139,127,240 (GRCm39)	nonsense	probably null
Aqua_regia	UTSW	4	139,180,030 (GRCm39)	nonsense	probably null
Eats	UTSW	4	139,190,886 (GRCm39)	missense	probably damaging	0.97
Hardened	UTSW	4	139,196,158 (GRCm39)	missense	probably damaging	1.00
Stoney	UTSW	4	139,171,998 (GRCm39)	missense	probably damaging	1.00
Uber	UTSW	4	139,206,373 (GRCm39)	critical splice donor site	probably null
BB001:Ubr4	UTSW	4	139,194,587 (GRCm39)	missense	unknown
BB011:Ubr4	UTSW	4	139,194,587 (GRCm39)	missense	unknown
P0019:Ubr4	UTSW	4	139,179,092 (GRCm39)	missense	probably damaging	1.00
PIT4544001:Ubr4	UTSW	4	139,129,871 (GRCm39)	missense	possibly damaging	0.93
R0001:Ubr4	UTSW	4	139,179,099 (GRCm39)	missense	probably damaging	1.00
R0002:Ubr4	UTSW	4	139,118,211 (GRCm39)	missense	probably damaging	1.00
R0006:Ubr4	UTSW	4	139,158,960 (GRCm39)	missense	probably benign	0.03
R0006:Ubr4	UTSW	4	139,158,960 (GRCm39)	missense	probably benign	0.03
R0027:Ubr4	UTSW	4	139,127,704 (GRCm39)	missense	probably damaging	0.99
R0027:Ubr4	UTSW	4	139,127,704 (GRCm39)	missense	probably damaging	0.99
R0030:Ubr4	UTSW	4	139,154,104 (GRCm39)	missense	probably damaging	1.00
R0044:Ubr4	UTSW	4	139,164,369 (GRCm39)	splice site	probably benign
R0044:Ubr4	UTSW	4	139,164,369 (GRCm39)	splice site	probably benign
R0088:Ubr4	UTSW	4	139,168,125 (GRCm39)	missense	probably damaging	1.00
R0131:Ubr4	UTSW	4	139,191,362 (GRCm39)	missense	possibly damaging	0.66
R0184:Ubr4	UTSW	4	139,172,573 (GRCm39)	missense	probably damaging	1.00
R0219:Ubr4	UTSW	4	139,157,568 (GRCm39)	missense	possibly damaging	0.64
R0227:Ubr4	UTSW	4	139,158,960 (GRCm39)	missense	probably benign	0.03
R0270:Ubr4	UTSW	4	139,206,746 (GRCm39)	splice site	probably benign
R0285:Ubr4	UTSW	4	139,168,112 (GRCm39)	missense	probably damaging	1.00
R0322:Ubr4	UTSW	4	139,149,729 (GRCm39)	missense	probably damaging	1.00
R0363:Ubr4	UTSW	4	139,119,171 (GRCm39)	missense	probably damaging	0.98
R0393:Ubr4	UTSW	4	139,138,171 (GRCm39)	splice site	probably benign
R0450:Ubr4	UTSW	4	139,157,534 (GRCm39)	missense	probably benign	0.14
R0504:Ubr4	UTSW	4	139,133,889 (GRCm39)	missense	probably damaging	1.00
R0504:Ubr4	UTSW	4	139,208,149 (GRCm39)	critical splice donor site	probably null
R0510:Ubr4	UTSW	4	139,157,534 (GRCm39)	missense	probably benign	0.14
R0513:Ubr4	UTSW	4	139,144,186 (GRCm39)	missense	possibly damaging	0.74
R0517:Ubr4	UTSW	4	139,119,435 (GRCm39)	missense	probably benign	0.00
R0558:Ubr4	UTSW	4	139,154,213 (GRCm39)	missense	probably benign	0.09
R0617:Ubr4	UTSW	4	139,206,373 (GRCm39)	critical splice donor site	probably null
R0636:Ubr4	UTSW	4	139,163,613 (GRCm39)	splice site	probably null
R0637:Ubr4	UTSW	4	139,126,926 (GRCm39)	missense	probably damaging	1.00
R0652:Ubr4	UTSW	4	139,128,637 (GRCm39)	missense	probably damaging	0.99
R0691:Ubr4	UTSW	4	139,151,217 (GRCm39)	missense	probably damaging	1.00
R0729:Ubr4	UTSW	4	139,212,631 (GRCm39)	missense	possibly damaging	0.66
R0735:Ubr4	UTSW	4	139,155,339 (GRCm39)	splice site	probably null
R0751:Ubr4	UTSW	4	139,164,509 (GRCm39)	splice site	probably benign
R0789:Ubr4	UTSW	4	139,137,582 (GRCm39)	critical splice donor site	probably null
R0825:Ubr4	UTSW	4	139,206,887 (GRCm39)	critical splice donor site	probably null
R0828:Ubr4	UTSW	4	139,177,864 (GRCm39)	splice site	probably benign
R1052:Ubr4	UTSW	4	139,182,771 (GRCm39)	missense	possibly damaging	0.83
R1184:Ubr4	UTSW	4	139,164,509 (GRCm39)	splice site	probably benign
R1222:Ubr4	UTSW	4	139,115,782 (GRCm39)	splice site	probably null
R1258:Ubr4	UTSW	4	139,154,225 (GRCm39)	missense	probably damaging	1.00
R1321:Ubr4	UTSW	4	139,187,434 (GRCm39)	missense	possibly damaging	0.46
R1385:Ubr4	UTSW	4	139,129,923 (GRCm39)	missense	probably benign	0.00
R1450:Ubr4	UTSW	4	139,195,339 (GRCm39)	missense	probably damaging	1.00
R1470:Ubr4	UTSW	4	139,148,537 (GRCm39)	splice site	probably null
R1470:Ubr4	UTSW	4	139,148,537 (GRCm39)	splice site	probably null
R1474:Ubr4	UTSW	4	139,156,890 (GRCm39)	missense	probably damaging	1.00
R1479:Ubr4	UTSW	4	139,153,151 (GRCm39)	missense	possibly damaging	0.95
R1534:Ubr4	UTSW	4	139,155,462 (GRCm39)	missense	possibly damaging	0.95
R1546:Ubr4	UTSW	4	139,144,238 (GRCm39)	nonsense	probably null
R1785:Ubr4	UTSW	4	139,151,256 (GRCm39)	missense	probably damaging	1.00
R1786:Ubr4	UTSW	4	139,151,256 (GRCm39)	missense	probably damaging	1.00
R1789:Ubr4	UTSW	4	139,120,364 (GRCm39)	missense	probably damaging	1.00
R1796:Ubr4	UTSW	4	139,155,907 (GRCm39)	missense	probably benign	0.25
R1800:Ubr4	UTSW	4	139,135,274 (GRCm39)	missense	probably damaging	0.99
R1801:Ubr4	UTSW	4	139,179,874 (GRCm39)	splice site	probably null
R1827:Ubr4	UTSW	4	139,153,008 (GRCm39)	critical splice acceptor site	probably null
R1887:Ubr4	UTSW	4	139,182,871 (GRCm39)	missense	probably damaging	1.00
R1966:Ubr4	UTSW	4	139,178,555 (GRCm39)	critical splice acceptor site	probably null
R2010:Ubr4	UTSW	4	139,207,963 (GRCm39)	missense	possibly damaging	0.92
R2049:Ubr4	UTSW	4	139,204,518 (GRCm39)	missense	probably damaging	0.97
R2069:Ubr4	UTSW	4	139,206,851 (GRCm39)	missense	possibly damaging	0.66
R2114:Ubr4	UTSW	4	139,156,922 (GRCm39)	nonsense	probably null
R2140:Ubr4	UTSW	4	139,204,518 (GRCm39)	missense	probably damaging	0.97
R2141:Ubr4	UTSW	4	139,204,518 (GRCm39)	missense	probably damaging	0.97
R2142:Ubr4	UTSW	4	139,204,518 (GRCm39)	missense	probably damaging	0.97
R2168:Ubr4	UTSW	4	139,137,960 (GRCm39)	missense	probably benign	0.25
R2237:Ubr4	UTSW	4	139,170,101 (GRCm39)	missense	probably damaging	1.00
R2249:Ubr4	UTSW	4	139,176,232 (GRCm39)	missense	probably damaging	1.00
R2261:Ubr4	UTSW	4	139,140,773 (GRCm39)	missense	probably damaging	0.99
R2264:Ubr4	UTSW	4	139,147,684 (GRCm39)	splice site	probably benign
R2353:Ubr4	UTSW	4	139,160,984 (GRCm39)	missense	possibly damaging	0.48
R2437:Ubr4	UTSW	4	139,200,853 (GRCm39)	missense	possibly damaging	0.90
R2496:Ubr4	UTSW	4	139,200,516 (GRCm39)	unclassified	probably benign
R2896:Ubr4	UTSW	4	139,182,955 (GRCm39)	splice site	probably null
R2922:Ubr4	UTSW	4	139,206,811 (GRCm39)	missense	possibly damaging	0.66
R2972:Ubr4	UTSW	4	139,133,847 (GRCm39)	missense	probably benign	0.22
R2973:Ubr4	UTSW	4	139,133,847 (GRCm39)	missense	probably benign	0.22
R2989:Ubr4	UTSW	4	139,190,869 (GRCm39)	missense	possibly damaging	0.89
R3176:Ubr4	UTSW	4	139,149,166 (GRCm39)	missense	probably benign	0.03
R3276:Ubr4	UTSW	4	139,149,166 (GRCm39)	missense	probably benign	0.03
R3772:Ubr4	UTSW	4	139,180,011 (GRCm39)	missense	possibly damaging	0.89
R3844:Ubr4	UTSW	4	139,186,437 (GRCm39)	missense	probably damaging	0.99
R3873:Ubr4	UTSW	4	139,151,301 (GRCm39)	missense	probably damaging	1.00
R3900:Ubr4	UTSW	4	139,206,373 (GRCm39)	critical splice donor site	probably null
R3951:Ubr4	UTSW	4	139,120,405 (GRCm39)	missense	probably damaging	1.00
R4020:Ubr4	UTSW	4	139,179,116 (GRCm39)	missense	probably damaging	0.98
R4178:Ubr4	UTSW	4	139,120,725 (GRCm39)	missense	probably damaging	1.00
R4308:Ubr4	UTSW	4	139,199,820 (GRCm39)	missense	possibly damaging	0.46
R4378:Ubr4	UTSW	4	139,137,751 (GRCm39)	missense	possibly damaging	0.76
R4400:Ubr4	UTSW	4	139,189,167 (GRCm39)	missense	possibly damaging	0.66
R4421:Ubr4	UTSW	4	139,189,167 (GRCm39)	missense	possibly damaging	0.66
R4462:Ubr4	UTSW	4	139,145,813 (GRCm39)	missense	possibly damaging	0.47
R4583:Ubr4	UTSW	4	139,108,164 (GRCm39)	missense	possibly damaging	0.82
R4611:Ubr4	UTSW	4	139,126,890 (GRCm39)	missense	possibly damaging	0.93
R4664:Ubr4	UTSW	4	139,133,829 (GRCm39)	missense	possibly damaging	0.56
R4671:Ubr4	UTSW	4	139,163,502 (GRCm39)	missense	possibly damaging	0.66
R4672:Ubr4	UTSW	4	139,138,027 (GRCm39)	missense	probably damaging	0.99
R4673:Ubr4	UTSW	4	139,138,027 (GRCm39)	missense	probably damaging	0.99
R4696:Ubr4	UTSW	4	139,135,983 (GRCm39)	missense	probably benign	0.09
R4701:Ubr4	UTSW	4	139,198,647 (GRCm39)	missense	possibly damaging	0.66
R4705:Ubr4	UTSW	4	139,177,840 (GRCm39)	missense	probably damaging	1.00
R4726:Ubr4	UTSW	4	139,209,890 (GRCm39)	missense	possibly damaging	0.46
R4728:Ubr4	UTSW	4	139,151,190 (GRCm39)	missense	probably damaging	1.00
R4783:Ubr4	UTSW	4	139,149,044 (GRCm39)	missense	possibly damaging	0.85
R4833:Ubr4	UTSW	4	139,129,857 (GRCm39)	missense	probably damaging	0.98
R4892:Ubr4	UTSW	4	139,155,828 (GRCm39)	missense	probably benign	0.14
R4936:Ubr4	UTSW	4	139,123,877 (GRCm39)	missense	probably damaging	0.98
R5000:Ubr4	UTSW	4	139,163,480 (GRCm39)	missense	probably damaging	0.98
R5114:Ubr4	UTSW	4	139,137,934 (GRCm39)	missense	probably damaging	0.99
R5189:Ubr4	UTSW	4	139,137,960 (GRCm39)	missense	probably benign	0.25
R5197:Ubr4	UTSW	4	139,195,408 (GRCm39)	missense	probably damaging	1.00
R5213:Ubr4	UTSW	4	139,129,877 (GRCm39)	nonsense	probably null
R5219:Ubr4	UTSW	4	139,204,543 (GRCm39)	nonsense	probably null
R5346:Ubr4	UTSW	4	139,155,802 (GRCm39)	missense	probably damaging	0.97
R5368:Ubr4	UTSW	4	139,124,839 (GRCm39)	intron	probably benign
R5442:Ubr4	UTSW	4	139,135,083 (GRCm39)	missense	probably damaging	1.00
R5527:Ubr4	UTSW	4	139,208,099 (GRCm39)	missense	possibly damaging	0.83
R5548:Ubr4	UTSW	4	139,187,401 (GRCm39)	missense	probably damaging	0.97
R5568:Ubr4	UTSW	4	139,119,349 (GRCm39)	missense	probably damaging	0.99
R5639:Ubr4	UTSW	4	139,179,959 (GRCm39)	missense	possibly damaging	0.66
R5643:Ubr4	UTSW	4	139,171,998 (GRCm39)	missense	probably damaging	1.00
R5663:Ubr4	UTSW	4	139,155,894 (GRCm39)	missense	possibly damaging	0.95
R5755:Ubr4	UTSW	4	139,187,406 (GRCm39)	missense	possibly damaging	0.66
R5781:Ubr4	UTSW	4	139,195,407 (GRCm39)	missense	probably damaging	1.00
R5784:Ubr4	UTSW	4	139,152,529 (GRCm39)	missense	probably damaging	1.00
R5817:Ubr4	UTSW	4	139,196,158 (GRCm39)	missense	probably damaging	1.00
R5872:Ubr4	UTSW	4	139,152,641 (GRCm39)	missense	probably damaging	0.98
R5891:Ubr4	UTSW	4	139,135,937 (GRCm39)	nonsense	probably null
R5901:Ubr4	UTSW	4	139,178,565 (GRCm39)	missense	probably damaging	1.00
R5958:Ubr4	UTSW	4	139,182,949 (GRCm39)	missense	probably damaging	1.00
R5974:Ubr4	UTSW	4	139,148,389 (GRCm39)	splice site	probably null
R6065:Ubr4	UTSW	4	139,148,549 (GRCm39)	missense	probably damaging	1.00
R6109:Ubr4	UTSW	4	139,144,675 (GRCm39)	missense	probably damaging	0.99
R6207:Ubr4	UTSW	4	139,148,559 (GRCm39)	missense	probably damaging	1.00
R6265:Ubr4	UTSW	4	139,179,951 (GRCm39)	missense	possibly damaging	0.90
R6319:Ubr4	UTSW	4	139,136,200 (GRCm39)	missense	possibly damaging	0.84
R6342:Ubr4	UTSW	4	139,156,850 (GRCm39)	missense	possibly damaging	0.88
R6434:Ubr4	UTSW	4	139,156,949 (GRCm39)	missense	probably damaging	1.00
R6437:Ubr4	UTSW	4	139,124,525 (GRCm39)	critical splice donor site	probably null
R6481:Ubr4	UTSW	4	139,159,062 (GRCm39)	missense	probably damaging	0.97
R6502:Ubr4	UTSW	4	139,171,982 (GRCm39)	missense	probably damaging	1.00
R6546:Ubr4	UTSW	4	139,141,705 (GRCm39)	missense	probably damaging	0.99
R6603:Ubr4	UTSW	4	139,182,897 (GRCm39)	missense	probably benign	0.17
R6648:Ubr4	UTSW	4	139,180,030 (GRCm39)	nonsense	probably null
R6649:Ubr4	UTSW	4	139,200,935 (GRCm39)	missense	possibly damaging	0.66
R6653:Ubr4	UTSW	4	139,200,935 (GRCm39)	missense	possibly damaging	0.66
R6668:Ubr4	UTSW	4	139,192,652 (GRCm39)	missense	probably damaging	1.00
R6770:Ubr4	UTSW	4	139,216,493 (GRCm39)	missense	unknown
R6772:Ubr4	UTSW	4	139,194,541 (GRCm39)	nonsense	probably null
R6857:Ubr4	UTSW	4	139,213,362 (GRCm39)	missense	possibly damaging	0.90
R6869:Ubr4	UTSW	4	139,194,538 (GRCm39)	missense	possibly damaging	0.93
R6912:Ubr4	UTSW	4	139,185,545 (GRCm39)	critical splice donor site	probably null
R6943:Ubr4	UTSW	4	139,164,442 (GRCm39)	nonsense	probably null
R6970:Ubr4	UTSW	4	139,133,839 (GRCm39)	nonsense	probably null
R6976:Ubr4	UTSW	4	139,120,388 (GRCm39)	missense	probably damaging	0.98
R7000:Ubr4	UTSW	4	139,141,715 (GRCm39)	missense	probably damaging	1.00
R7017:Ubr4	UTSW	4	139,120,401 (GRCm39)	missense	probably damaging	0.99
R7165:Ubr4	UTSW	4	139,177,824 (GRCm39)	missense
R7222:Ubr4	UTSW	4	139,190,684 (GRCm39)	missense	unknown
R7241:Ubr4	UTSW	4	139,170,725 (GRCm39)	missense	probably damaging	1.00
R7343:Ubr4	UTSW	4	139,140,749 (GRCm39)	missense	probably benign	0.09
R7367:Ubr4	UTSW	4	139,180,002 (GRCm39)	missense	unknown
R7393:Ubr4	UTSW	4	139,154,096 (GRCm39)	missense	probably damaging	1.00
R7432:Ubr4	UTSW	4	139,115,693 (GRCm39)	missense	probably damaging	1.00
R7448:Ubr4	UTSW	4	139,189,778 (GRCm39)	missense	unknown
R7502:Ubr4	UTSW	4	139,139,983 (GRCm39)	missense	possibly damaging	0.72
R7514:Ubr4	UTSW	4	139,179,966 (GRCm39)	missense	unknown
R7526:Ubr4	UTSW	4	139,149,728 (GRCm39)	missense	probably benign	0.00
R7529:Ubr4	UTSW	4	139,149,728 (GRCm39)	missense	probably benign	0.00
R7666:Ubr4	UTSW	4	139,140,791 (GRCm39)	missense	possibly damaging	0.81
R7699:Ubr4	UTSW	4	139,136,178 (GRCm39)	nonsense	probably null
R7700:Ubr4	UTSW	4	139,136,178 (GRCm39)	nonsense	probably null
R7726:Ubr4	UTSW	4	139,186,231 (GRCm39)	missense	unknown
R7753:Ubr4	UTSW	4	139,197,603 (GRCm39)	missense	unknown
R7810:Ubr4	UTSW	4	139,142,394 (GRCm39)	missense
R7837:Ubr4	UTSW	4	139,120,462 (GRCm39)	nonsense	probably null
R7868:Ubr4	UTSW	4	139,187,344 (GRCm39)	missense	unknown
R7872:Ubr4	UTSW	4	139,120,373 (GRCm39)	missense	possibly damaging	0.94
R7887:Ubr4	UTSW	4	139,135,121 (GRCm39)	missense	probably damaging	0.99
R7924:Ubr4	UTSW	4	139,194,587 (GRCm39)	missense	unknown
R7980:Ubr4	UTSW	4	139,145,717 (GRCm39)	missense
R7982:Ubr4	UTSW	4	139,155,519 (GRCm39)	critical splice donor site	probably null
R8005:Ubr4	UTSW	4	139,139,941 (GRCm39)	missense	probably damaging	0.98
R8054:Ubr4	UTSW	4	139,195,413 (GRCm39)	missense	unknown
R8094:Ubr4	UTSW	4	139,168,007 (GRCm39)	missense	probably damaging	0.97
R8151:Ubr4	UTSW	4	139,130,112 (GRCm39)	missense	probably damaging	0.97
R8183:Ubr4	UTSW	4	139,209,782 (GRCm39)	missense	unknown
R8252:Ubr4	UTSW	4	139,200,528 (GRCm39)	missense	unknown
R8505:Ubr4	UTSW	4	139,156,880 (GRCm39)	missense
R8519:Ubr4	UTSW	4	139,143,958 (GRCm39)	missense	probably damaging	0.97
R8716:Ubr4	UTSW	4	139,196,164 (GRCm39)	missense	unknown
R8720:Ubr4	UTSW	4	139,208,149 (GRCm39)	critical splice donor site	probably null
R8749:Ubr4	UTSW	4	139,129,935 (GRCm39)	missense	probably damaging	0.98
R8768:Ubr4	UTSW	4	139,149,076 (GRCm39)	missense
R8789:Ubr4	UTSW	4	139,137,494 (GRCm39)	missense	possibly damaging	0.76
R8879:Ubr4	UTSW	4	139,137,829 (GRCm39)	missense	probably benign	0.06
R8937:Ubr4	UTSW	4	139,190,886 (GRCm39)	missense	probably damaging	0.97
R9006:Ubr4	UTSW	4	139,172,003 (GRCm39)	critical splice donor site	probably null
R9116:Ubr4	UTSW	4	139,145,785 (GRCm39)	missense
R9134:Ubr4	UTSW	4	139,127,755 (GRCm39)	critical splice donor site	probably null
R9251:Ubr4	UTSW	4	139,177,636 (GRCm39)	missense
R9340:Ubr4	UTSW	4	139,182,763 (GRCm39)	missense	unknown
R9384:Ubr4	UTSW	4	139,194,631 (GRCm39)	missense	unknown
R9389:Ubr4	UTSW	4	139,153,235 (GRCm39)	missense
R9393:Ubr4	UTSW	4	139,212,613 (GRCm39)	missense	unknown
R9531:Ubr4	UTSW	4	139,135,217 (GRCm39)	missense	probably benign	0.00
R9573:Ubr4	UTSW	4	139,148,450 (GRCm39)	missense
R9604:Ubr4	UTSW	4	139,159,827 (GRCm39)	critical splice donor site	probably null
R9613:Ubr4	UTSW	4	139,149,073 (GRCm39)	missense
R9623:Ubr4	UTSW	4	139,159,024 (GRCm39)	missense	probably benign	0.00
R9651:Ubr4	UTSW	4	139,206,859 (GRCm39)	missense	unknown
R9685:Ubr4	UTSW	4	139,191,341 (GRCm39)	missense	unknown
R9698:Ubr4	UTSW	4	139,167,975 (GRCm39)	missense
R9700:Ubr4	UTSW	4	139,119,388 (GRCm39)	missense	probably damaging	0.97
R9727:Ubr4	UTSW	4	139,140,735 (GRCm39)	missense	probably damaging	0.98
R9766:Ubr4	UTSW	4	139,194,595 (GRCm39)	missense	unknown
T0975:Ubr4	UTSW	4	139,179,092 (GRCm39)	missense	probably damaging	1.00
X0028:Ubr4	UTSW	4	139,137,582 (GRCm39)	critical splice donor site	probably null
Z1176:Ubr4	UTSW	4	139,137,456 (GRCm39)	missense	probably damaging	1.00
Z1176:Ubr4	UTSW	4	139,129,971 (GRCm39)	missense	probably benign	0.04
Z1176:Ubr4	UTSW	4	139,127,696 (GRCm39)	missense	probably damaging	1.00
Z1177:Ubr4	UTSW	4	139,177,792 (GRCm39)	missense
Z1186:Ubr4	UTSW	4	139,137,964 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- AGGCAAGGACTCCCATCATTGAGG -3'
(R):5'- CATCTGTTGTAGCCCCATGAGACG -3'

Sequencing Primer
(F):5'- TCTccctctcatatctatgacacg -3'
(R):5'- ATGAGACGGCTCCAGTCTG -3'

Posted On

2013-07-11