Mutagenetix > Incidental Mutation

Incidental Mutation 'R7510:Zfp160'

582055

Institutional Source

Beutler Lab

Gene Symbol

Zfp160

Ensembl Gene

ENSMUSG00000067942

Gene Name

zinc finger protein 160

Synonyms

6720480D16Rik, 6720480D16Rik

MMRRC Submission

045583-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7510 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21008903-21043070 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 21026393 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Lysine at position 402 (E402K)

Ref Sequence

ENSEMBL: ENSMUSP00000086191 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088811] [ENSMUST00000231482] [ENSMUST00000232320] [ENSMUST00000232354] [ENSMUST00000232473] [ENSMUST00000232663]

AlphaFold

E9Q459

Predicted Effect

probably benign
Transcript: ENSMUST00000088811
AA Change: E402K

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000086191
Gene: ENSMUSG00000067942
AA Change: E402K

Domain	Start	End	E-Value	Type
KRAB	8	68	1.91e-29	SMART
low complexity region	100	110	N/A	INTRINSIC
ZnF_C2H2	146	168	1.69e-3	SMART
ZnF_C2H2	174	196	2.91e-2	SMART
ZnF_C2H2	202	224	1.4e-4	SMART
ZnF_C2H2	230	252	3.89e-3	SMART
ZnF_C2H2	258	280	1.72e-4	SMART
ZnF_C2H2	286	308	4.94e-5	SMART
ZnF_C2H2	314	336	2.12e-4	SMART
ZnF_C2H2	342	364	1.12e-3	SMART
ZnF_C2H2	370	392	1.2e-3	SMART
ZnF_C2H2	398	420	6.42e-4	SMART
ZnF_C2H2	426	448	9.08e-4	SMART
ZnF_C2H2	454	476	1.84e-4	SMART
ZnF_C2H2	482	504	1.5e-4	SMART
ZnF_C2H2	510	532	3.44e-4	SMART
ZnF_C2H2	538	560	1.12e-3	SMART
ZnF_C2H2	566	588	2.27e-4	SMART
ZnF_C2H2	594	616	1.04e-3	SMART
ZnF_C2H2	622	644	1.13e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000231482

Predicted Effect

probably benign
Transcript: ENSMUST00000232320

Predicted Effect

probably benign
Transcript: ENSMUST00000232354

Predicted Effect

probably benign
Transcript: ENSMUST00000232473

Predicted Effect

probably benign
Transcript: ENSMUST00000232663

Meta Mutation Damage Score

0.4503

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.0%

Validation Efficiency

97% (65/67)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Atp2b1	C	T	10: 98,993,896 (GRCm38)	R320C	probably benign	Het
Bend4	A	G	5: 67,427,384 (GRCm38)	F66L	unknown	Het
Brca2	T	C	5: 150,536,691 (GRCm38)	V477A	possibly damaging	Het
Brms1l	A	T	12: 55,845,322 (GRCm38)	K134*	probably null	Het
Catsper1	A	T	19: 5,339,550 (GRCm38)	T498S	probably benign	Het
Ccdc177	C	A	12: 80,757,683 (GRCm38)	V606L	unknown	Het
D030056L22Rik	G	T	19: 18,713,489 (GRCm38)	A56S	possibly damaging	Het
Disp1	T	C	1: 183,088,411 (GRCm38)	N815S	probably damaging	Het
Dsc2	C	T	18: 20,032,274 (GRCm38)	G881R	possibly damaging	Het
Fancf	A	G	7: 51,862,205 (GRCm38)	V17A	probably damaging	Het
Fastkd2	T	A	1: 63,737,789 (GRCm38)	H361Q	possibly damaging	Het
Furin	A	G	7: 80,393,585 (GRCm38)	S293P	probably damaging	Het
Ghsr	A	T	3: 27,372,374 (GRCm38)	D193V	probably benign	Het
Gm14403	T	A	2: 177,508,610 (GRCm38)	N116K	probably benign	Het
Gpr31b	A	G	17: 13,051,670 (GRCm38)	L204P	probably damaging	Het
Hexim1	A	G	11: 103,117,241 (GRCm38)	E107G	probably benign	Het
Hspa14	T	C	2: 3,498,122 (GRCm38)	S212G	probably benign	Het
Il18r1	T	A	1: 40,474,875 (GRCm38)	H80Q	probably benign	Het
Itpr3	A	G	17: 27,089,039 (GRCm38)	T267A	probably damaging	Het
Kidins220	T	A	12: 24,992,269 (GRCm38)	H146Q	possibly damaging	Het
Larp4	T	C	15: 99,993,377 (GRCm38)	F228L	probably benign	Het
Ltbp1	T	A	17: 75,352,717 (GRCm38)	V1288E	probably damaging	Het
Madd	T	C	2: 91,177,976 (GRCm38)	T194A	possibly damaging	Het
Mlana	G	A	19: 29,704,672 (GRCm38)	G42S	probably benign	Het
Mlxipl	A	G	5: 135,133,118 (GRCm38)	E548G	possibly damaging	Het
Mmab	A	T	5: 114,435,222 (GRCm38)	C228S	probably benign	Het
Mmp1a	TG	TGG	9: 7,465,083 (GRCm38)		probably null	Het
Nrf1	C	T	6: 30,151,634 (GRCm38)	T490I	possibly damaging	Het
Numbl	A	G	7: 27,271,987 (GRCm38)		probably null	Het
Olfr1014	T	A	2: 85,776,809 (GRCm38)	V75D	probably damaging	Het
Olfr111	T	A	17: 37,530,589 (GRCm38)	I204N	probably damaging	Het
Olfr1148	T	A	2: 87,833,528 (GRCm38)	I163K	probably damaging	Het
Olfr1350	C	T	7: 6,569,961 (GRCm38)		probably benign	Het
Olfr155	A	G	4: 43,854,482 (GRCm38)	T58A	probably benign	Het
Olfr803	G	T	10: 129,691,920 (GRCm38)	N40K	probably damaging	Het
Papln	G	A	12: 83,772,173 (GRCm38)	D96N	probably damaging	Het
Pcdhb14	T	C	18: 37,449,592 (GRCm38)	Y584H	probably damaging	Het
Pde7b	T	C	10: 20,413,015 (GRCm38)	D310G	possibly damaging	Het
Plin5	T	A	17: 56,113,975 (GRCm38)	H230L	probably damaging	Het
Ppp1r13l	A	C	7: 19,368,801 (GRCm38)	E47A	possibly damaging	Het
Prdm5	C	T	6: 65,927,992 (GRCm38)	H536Y	probably damaging	Het
Prickle2	T	C	6: 92,376,470 (GRCm38)	R728G	possibly damaging	Het
Prkca	A	T	11: 107,983,994 (GRCm38)	V374E	possibly damaging	Het
Prss3	A	T	6: 41,375,110 (GRCm38)	L73*	probably null	Het
Prss51	G	A	14: 64,096,040 (GRCm38)	D33N	probably damaging	Het
Rfwd3	A	G	8: 111,280,027 (GRCm38)	V479A	probably damaging	Het
Rpl36a-ps1	G	A	14: 98,994,230 (GRCm38)	T24I	probably benign	Het
Rps6ka5	T	C	12: 100,616,068 (GRCm38)	I182V	possibly damaging	Het
Saa2	T	A	7: 46,753,509 (GRCm38)	D61E	probably damaging	Het
Samd3	A	T	10: 26,230,108 (GRCm38)	I22F	probably benign	Het
Sap130	C	T	18: 31,667,004 (GRCm38)	P403L	probably damaging	Het
Sap130	A	G	18: 31,711,215 (GRCm38)	T813A	probably damaging	Het
Scfd2	A	G	5: 74,212,327 (GRCm38)	F629S	probably damaging	Het
Sec61a1	A	T	6: 88,512,603 (GRCm38)	F119I	probably benign	Het
Serpinb9	T	A	13: 33,010,785 (GRCm38)	F175I	probably damaging	Het
Slc12a3	G	T	8: 94,365,849 (GRCm38)	C966F	probably damaging	Het
Sptbn4	A	G	7: 27,428,268 (GRCm38)	V169A	probably benign	Het
Synj1	A	G	16: 90,938,677 (GRCm38)	S1463P	probably benign	Het
Tfec	T	A	6: 16,835,233 (GRCm38)	H182L	probably benign	Het
Tigd5	T	C	15: 75,910,419 (GRCm38)	V210A	probably benign	Het
Tssc4	G	A	7: 143,069,981 (GRCm38)	E9K	possibly damaging	Het
Txk	C	G	5: 72,736,383 (GRCm38)	C18S	unknown	Het
Uaca	G	A	9: 60,850,205 (GRCm38)		probably null	Het
Vmn2r1	A	C	3: 64,086,501 (GRCm38)	K89N	probably damaging	Het

Other mutations in Zfp160

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00714:Zfp160	APN	17	21,026,702 (GRCm38)	missense	probably benign
IGL01019:Zfp160	APN	17	21,020,826 (GRCm38)	missense	possibly damaging	0.68
IGL02430:Zfp160	APN	17	21,025,530 (GRCm38)	missense	possibly damaging	0.76
R0412:Zfp160	UTSW	17	21,026,877 (GRCm38)	missense	probably damaging	0.97
R0600:Zfp160	UTSW	17	21,027,006 (GRCm38)	missense	probably benign	0.00
R2146:Zfp160	UTSW	17	21,026,982 (GRCm38)	missense	probably benign	0.13
R2157:Zfp160	UTSW	17	21,020,828 (GRCm38)	missense	probably benign	0.23
R2411:Zfp160	UTSW	17	21,025,745 (GRCm38)	missense	possibly damaging	0.94
R2904:Zfp160	UTSW	17	21,025,649 (GRCm38)	missense	probably benign	0.00
R4249:Zfp160	UTSW	17	21,025,738 (GRCm38)	missense	probably benign	0.11
R4896:Zfp160	UTSW	17	21,020,081 (GRCm38)	missense	probably benign	0.00
R5106:Zfp160	UTSW	17	21,026,761 (GRCm38)	missense	probably damaging	0.99
R5342:Zfp160	UTSW	17	21,020,733 (GRCm38)	missense	possibly damaging	0.95
R5352:Zfp160	UTSW	17	21,026,852 (GRCm38)	missense	probably benign	0.02
R6193:Zfp160	UTSW	17	21,026,862 (GRCm38)	missense	probably benign	0.24
R6230:Zfp160	UTSW	17	21,026,445 (GRCm38)	missense	probably benign	0.38
R6753:Zfp160	UTSW	17	21,020,734 (GRCm38)	missense	probably benign	0.02
R6928:Zfp160	UTSW	17	21,041,462 (GRCm38)	missense	probably benign	0.04
R7040:Zfp160	UTSW	17	21,026,532 (GRCm38)	missense	probably damaging	1.00
R7255:Zfp160	UTSW	17	21,025,487 (GRCm38)	missense	probably benign	0.18
R7497:Zfp160	UTSW	17	21,026,193 (GRCm38)	missense	probably benign	0.08
R7540:Zfp160	UTSW	17	21,025,660 (GRCm38)	nonsense	probably null
R7627:Zfp160	UTSW	17	21,027,008 (GRCm38)	missense	probably damaging	0.99
R8169:Zfp160	UTSW	17	21,027,036 (GRCm38)	missense	probably damaging	0.97
R8240:Zfp160	UTSW	17	21,026,088 (GRCm38)	missense	probably damaging	0.99
R8330:Zfp160	UTSW	17	21,026,051 (GRCm38)	missense	probably damaging	1.00
R8367:Zfp160	UTSW	17	21,025,542 (GRCm38)	missense	probably benign	0.22
R8802:Zfp160	UTSW	17	21,026,605 (GRCm38)	missense	probably damaging	1.00
R9183:Zfp160	UTSW	17	21,020,092 (GRCm38)	missense	possibly damaging	0.84
R9556:Zfp160	UTSW	17	21,026,769 (GRCm38)	missense	probably benign	0.03
R9695:Zfp160	UTSW	17	21,025,484 (GRCm38)	missense	possibly damaging	0.53
Z1177:Zfp160	UTSW	17	21,026,890 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGGTGCAATGAGTGTGGG -3'
(R):5'- TCATTGCATCTGTAAGGTTTCTCTC -3'

Sequencing Primer
(F):5'- CCCTACAAATGTTCAGAGTGTGGC -3'
(R):5'- AGTCTGACTGAAGACCTTGC -3'

Posted On

2019-10-17