Mutagenetix > Incidental Mutation

Incidental Mutation 'R7514:Vmn2r104'

582357

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r104

Ensembl Gene

ENSMUSG00000090315

Gene Name

vomeronasal 2, receptor 104

Synonyms

V2r7

MMRRC Submission

045587-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R7514 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

20029425-20048205 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 20029529 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 827 (F827L)

Ref Sequence

ENSEMBL: ENSMUSP00000129895 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168050]

AlphaFold

E9Q2J5

Predicted Effect

probably damaging
Transcript: ENSMUST00000168050
AA Change: F827L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000129895
Gene: ENSMUSG00000090315
AA Change: F827L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	85	457	4e-38	PFAM
Pfam:NCD3G	512	565	2.1e-20	PFAM
Pfam:7tm_3	598	833	1.7e-52	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427D14Rik	A	G	11: 72,195,802	L261P	probably damaging	Het
A4gnt	A	G	9: 99,620,545	I253V	probably benign	Het
Acap2	A	T	16: 31,154,567		probably null	Het
Adora2b	G	T	11: 62,265,320	M198I	probably damaging	Het
Akap5	C	A	12: 76,328,529	T245K	probably benign	Het
Aldh1a2	T	C	9: 71,284,963	I399T	probably damaging	Het
Ank2	G	A	3: 127,025,603	S473L	probably benign	Het
Anln	A	T	9: 22,360,857	D655E	probably damaging	Het
Arhgef10	T	C	8: 14,975,956	V820A	probably benign	Het
Arid1b	A	G	17: 5,341,714	K1787E	probably benign	Het
Art4	T	C	6: 136,854,741	H134R	probably benign	Het
Borcs6	G	A	11: 69,060,584	V263M	probably damaging	Het
C8a	A	T	4: 104,846,050	M314K	possibly damaging	Het
Cbfa2t3	T	G	8: 122,635,126	M386L	probably damaging	Het
Ccdc170	T	A	10: 4,546,839	V459E	probably benign	Het
Cdh4	T	A	2: 179,890,843	N699K	possibly damaging	Het
Cdk12	T	C	11: 98,222,658	L756P	unknown	Het
Chsy1	A	G	7: 66,172,120	D701G	probably damaging	Het
Cnot9	A	G	1: 74,528,762	T270A	probably benign	Het
Crat	C	T	2: 30,404,565	R497Q	probably benign	Het
Cyb5r1	A	G	1: 134,410,530	E228G	probably damaging	Het
D630003M21Rik	A	G	2: 158,217,353	L209P	probably damaging	Het
Dennd2c	T	A	3: 103,163,062	D791E	probably benign	Het
Dnah14	T	C	1: 181,628,067	I919T	probably damaging	Het
Dpp8	T	C	9: 65,078,754	L842S	probably damaging	Het
Ern1	A	G	11: 106,409,893		probably null	Het
Exo1	T	C	1: 175,906,666		probably null	Het
Fam161b	T	G	12: 84,357,738	E56A	possibly damaging	Het
Fbrsl1	T	C	5: 110,432,933	T153A	probably benign	Het
Fcgr3	T	G	1: 171,059,343	D4A	probably benign	Het
Gltp	C	T	5: 114,670,460	A193T	probably benign	Het
Gm10031	A	G	1: 156,524,754	D175G	probably benign	Het
Gm13178	A	T	4: 144,703,228	V397D	possibly damaging	Het
Gm3159	A	T	14: 4,399,690	S142C	probably damaging	Het
Gphn	T	C	12: 78,626,165	V485A	probably damaging	Het
Grik2	A	T	10: 49,523,808	N275K	probably damaging	Het
Gstt3	G	T	10: 75,776,791	Q102K	probably damaging	Het
Hivep3	T	A	4: 120,096,855	F789L	possibly damaging	Het
Ing5	A	G	1: 93,816,442	N157D	possibly damaging	Het
Itga3	C	T	11: 95,065,896	W177*	probably null	Het
Jag2	T	C	12: 112,929,052	T83A	probably benign	Het
Krt90	G	A	15: 101,553,170	T532I	unknown	Het
Lcn2	A	G	2: 32,387,849		probably null	Het
Lrig2	A	G	3: 104,465,760	S602P	probably damaging	Het
Lsm1	A	C	8: 25,792,209	R33S	probably damaging	Het
Mast4	A	T	13: 102,787,426	Y492*	probably null	Het
Mcm3	A	T	1: 20,805,896	L658Q	probably benign	Het
Myh4	G	A	11: 67,243,322		probably null	Het
Nck2	T	C	1: 43,569,221	V341A	probably benign	Het
Nucb1	T	C	7: 45,501,718		probably null	Het
Nup210l	G	C	3: 90,210,459		probably null	Het
Olfr1039	A	T	2: 86,131,628	F12I	probably damaging	Het
Olfr3	A	G	2: 36,812,639	I151T	probably benign	Het
Olfr837	A	T	9: 19,137,865	S291C	possibly damaging	Het
Olfr912	T	A	9: 38,582,051	M258K	probably damaging	Het
Olfr921	G	A	9: 38,775,678	C141Y	probably damaging	Het
Pla2g4a	G	A	1: 149,851,362	P556S	probably damaging	Het
Plat	T	C	8: 22,775,642	C234R	probably damaging	Het
Ppfia1	T	C	7: 144,517,713	I321V	probably benign	Het
Prrc1	T	A	18: 57,363,253	V92E	probably benign	Het
Prss3	A	G	6: 41,373,914	V214A	probably damaging	Het
Ptprg	G	T	14: 12,179,342	K786N	possibly damaging	Het
Rabgap1	G	T	2: 37,537,342	G645V	probably damaging	Het
Rfx4	T	C	10: 84,880,226	S470P	probably damaging	Het
Rin3	C	T	12: 102,369,650	Q607*	probably null	Het
Sema3a	A	G	5: 13,523,126	H207R	probably benign	Het
Serpinb12	G	A	1: 106,950,804	E181K	probably damaging	Het
Serpinb6c	G	A	13: 33,897,403	Q88*	probably null	Het
Shmt1	A	C	11: 60,801,986	C90W	probably damaging	Het
Slc16a13	C	T	11: 70,218,884	V264M	probably damaging	Het
Slc17a8	G	T	10: 89,592,107	P286Q	probably damaging	Het
Slc27a6	C	T	18: 58,612,221	Q576*	probably null	Het
Slc30a9	T	C	5: 67,348,078	S470P	possibly damaging	Het
Slc44a2	G	T	9: 21,342,472	K136N	possibly damaging	Het
Smarca5	A	T	8: 80,717,534	H534Q	probably damaging	Het
Son	T	G	16: 91,654,860	L165R	probably damaging	Het
Sox10	G	A	15: 79,156,221	P373L	probably benign	Het
Sp100	C	T	1: 85,681,139	R330*	probably null	Het
Srrm4	A	G	5: 116,446,511	L500P	probably damaging	Het
Tap1	T	C	17: 34,196,665	L689P	probably damaging	Het
Tfg	A	G	16: 56,705,609		probably null	Het
Tjp2	C	T	19: 24,111,522	V677I	probably benign	Het
Tmprss11g	T	G	5: 86,497,317	D85A	probably damaging	Het
Tnfsf9	T	C	17: 57,107,238	S222P	probably damaging	Het
Trank1	A	G	9: 111,364,756	N616S	probably damaging	Het
Tubgcp6	C	A	15: 89,120,525	W297L	probably damaging	Het
Twist1	T	C	12: 33,958,356	S127P	probably damaging	Het
Ubr4	T	C	4: 139,452,655	I247T	unknown	Het
Ubr5	G	A	15: 37,988,237	T2153M		Het
Utrn	A	G	10: 12,698,089	V1079A	probably benign	Het
Vcan	T	C	13: 89,704,118	T908A	probably damaging	Het
Vmn2r111	T	A	17: 22,548,399	T706S	probably benign	Het
Vmn2r114	T	C	17: 23,308,061	D499G	probably null	Het
Vmn2r92	T	C	17: 18,171,271	S512P	probably damaging	Het
Vps4b	A	T	1: 106,780,502		probably null	Het
Vwa8	A	C	14: 78,982,234		probably null	Het
Wnt10b	T	C	15: 98,774,164	Q224R	probably benign	Het
Ylpm1	C	T	12: 85,030,494	P1331L	possibly damaging	Het
Zfhx4	G	A	3: 5,242,207	M164I	possibly damaging	Het
Zfp51	A	G	17: 21,463,500	T126A	probably benign	Het
Zfp609	A	G	9: 65,706,136	V339A	probably benign	Het
Zfp687	C	T	3: 95,007,530	R1220H	probably damaging	Het
Zfyve21	C	T	12: 111,823,815	L84F	probably damaging	Het

Other mutations in Vmn2r104

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Vmn2r104	APN	17	20038239	missense	probably damaging	0.98
IGL01098:Vmn2r104	APN	17	20048096	missense	probably benign	0.27
IGL01333:Vmn2r104	APN	17	20042793	missense	probably benign	0.17
IGL01527:Vmn2r104	APN	17	20042896	missense	possibly damaging	0.82
IGL01773:Vmn2r104	APN	17	20040668	missense	probably benign	0.10
IGL01939:Vmn2r104	APN	17	20029925	missense	probably damaging	0.99
IGL02121:Vmn2r104	APN	17	20041794	nonsense	probably null
IGL02305:Vmn2r104	APN	17	20042856	missense	probably benign	0.09
IGL02374:Vmn2r104	APN	17	20042786	missense	probably benign	0.34
IGL03260:Vmn2r104	APN	17	20042821	missense	probably benign	0.05
IGL03366:Vmn2r104	APN	17	20029604	missense	probably damaging	1.00
R0091:Vmn2r104	UTSW	17	20041813	missense	possibly damaging	0.79
R0125:Vmn2r104	UTSW	17	20029807	missense	probably damaging	0.98
R0257:Vmn2r104	UTSW	17	20029627	missense	probably damaging	1.00
R0381:Vmn2r104	UTSW	17	20048002	nonsense	probably null
R0709:Vmn2r104	UTSW	17	20042904	missense	probably damaging	1.00
R0786:Vmn2r104	UTSW	17	20042725	missense	probably benign
R1575:Vmn2r104	UTSW	17	20042215	missense	probably damaging	1.00
R1827:Vmn2r104	UTSW	17	20042235	missense	probably damaging	0.97
R1932:Vmn2r104	UTSW	17	20040769	missense	probably damaging	1.00
R1956:Vmn2r104	UTSW	17	20042051	missense	probably damaging	0.98
R2203:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2205:Vmn2r104	UTSW	17	20029821	missense	probably benign	0.05
R2859:Vmn2r104	UTSW	17	20048193	missense	possibly damaging	0.82
R3701:Vmn2r104	UTSW	17	20029556	missense	probably damaging	1.00
R3834:Vmn2r104	UTSW	17	20029921	missense	probably benign	0.02
R4151:Vmn2r104	UTSW	17	20029885	missense	probably damaging	1.00
R4470:Vmn2r104	UTSW	17	20042241	missense	probably damaging	1.00
R4625:Vmn2r104	UTSW	17	20048181	missense	probably benign	0.00
R4754:Vmn2r104	UTSW	17	20040768	nonsense	probably null
R4911:Vmn2r104	UTSW	17	20030026	missense	probably benign	0.00
R5270:Vmn2r104	UTSW	17	20038266	missense	probably damaging	1.00
R5279:Vmn2r104	UTSW	17	20041884	missense	probably benign	0.07
R5311:Vmn2r104	UTSW	17	20029901	missense	probably damaging	1.00
R5370:Vmn2r104	UTSW	17	20030188	missense	probably damaging	0.97
R5461:Vmn2r104	UTSW	17	20030081	missense	probably damaging	1.00
R5683:Vmn2r104	UTSW	17	20040719	nonsense	probably null
R5795:Vmn2r104	UTSW	17	20030110	missense	probably benign	0.02
R5795:Vmn2r104	UTSW	17	20030282	missense	possibly damaging	0.89
R5970:Vmn2r104	UTSW	17	20029471	missense	probably benign	0.01
R5983:Vmn2r104	UTSW	17	20041708	missense	probably damaging	1.00
R5992:Vmn2r104	UTSW	17	20029485	missense	probably damaging	1.00
R6066:Vmn2r104	UTSW	17	20038311	missense	possibly damaging	0.69
R6156:Vmn2r104	UTSW	17	20041647	missense	probably damaging	1.00
R6182:Vmn2r104	UTSW	17	20030245	missense	probably benign	0.16
R6245:Vmn2r104	UTSW	17	20041567	missense	possibly damaging	0.69
R6333:Vmn2r104	UTSW	17	20029586	missense	probably benign	0.30
R6573:Vmn2r104	UTSW	17	20042225	missense	probably damaging	1.00
R7101:Vmn2r104	UTSW	17	20030096	missense	possibly damaging	0.65
R7123:Vmn2r104	UTSW	17	20040826	missense	probably benign	0.12
R7485:Vmn2r104	UTSW	17	20029475	missense	probably benign	0.01
R7634:Vmn2r104	UTSW	17	20041709	missense	possibly damaging	0.48
R7958:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8031:Vmn2r104	UTSW	17	20042786	missense	probably benign	0.34
R8094:Vmn2r104	UTSW	17	20030221	missense	possibly damaging	0.77
R8191:Vmn2r104	UTSW	17	20030203	missense	possibly damaging	0.89
R8308:Vmn2r104	UTSW	17	20040778	missense	possibly damaging	0.55
R8691:Vmn2r104	UTSW	17	20041848	missense	probably damaging	0.98
R8795:Vmn2r104	UTSW	17	20042726	missense	probably benign
R8900:Vmn2r104	UTSW	17	20041662	missense	probably damaging	0.99
R8913:Vmn2r104	UTSW	17	20029706	missense	probably damaging	1.00
R9180:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9199:Vmn2r104	UTSW	17	20041835	missense	probably damaging	0.99
R9282:Vmn2r104	UTSW	17	20040836	missense	probably damaging	1.00
R9303:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9305:Vmn2r104	UTSW	17	20048177	missense	possibly damaging	0.90
R9322:Vmn2r104	UTSW	17	20042825	missense	probably benign	0.00
R9325:Vmn2r104	UTSW	17	20048171	missense	possibly damaging	0.95
R9414:Vmn2r104	UTSW	17	20029988	missense	probably damaging	0.99
R9785:Vmn2r104	UTSW	17	20048147	missense	probably benign
RF007:Vmn2r104	UTSW	17	20048040	missense	probably benign	0.36
Z1177:Vmn2r104	UTSW	17	20029789	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AAGCATCAGGTCTTCAACTCAG -3'
(R):5'- TGGCCTTCTTGTCTAGAAATTTGC -3'

Sequencing Primer
(F):5'- TCAGGTCTTCAACTCAGAGATATTG -3'
(R):5'- TTGCCTGATACATTCAATGAATCC -3'

Posted On

2019-10-17