Incidental Mutation 'R7526:Flrt3'
ID 582965
Institutional Source Beutler Lab
Gene Symbol Flrt3
Ensembl Gene ENSMUSG00000051379
Gene Name fibronectin leucine rich transmembrane protein 3
Synonyms 5530600M07Rik, C430047I10Rik
MMRRC Submission 045598-MU
Accession Numbers
Essential gene? Essential (E-score: 1.000) question?
Stock # R7526 (G1)
Quality Score 225.009
Status Validated
Chromosome 2
Chromosomal Location 140650914-140671469 bp(-) (GRCm38)
Type of Mutation missense
DNA Base Change (assembly) T to A at 140660206 bp (GRCm38)
Zygosity Heterozygous
Amino Acid Change Threonine to Serine at position 501 (T501S)
Ref Sequence ENSEMBL: ENSMUSP00000053399 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000056760] [ENSMUST00000078027] [ENSMUST00000110057] [ENSMUST00000110063] [ENSMUST00000110064] [ENSMUST00000110067]
AlphaFold Q8BGT1
PDB Structure FLRT3 LRR domain [X-RAY DIFFRACTION]
Predicted Effect probably damaging
Transcript: ENSMUST00000056760
AA Change: T501S

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000053399
Gene: ENSMUSG00000051379
AA Change: T501S

DomainStartEndE-ValueType
LRRNT 30 62 3.12e-6 SMART
LRR 82 105 1.03e2 SMART
LRR 127 152 8.26e1 SMART
LRR 156 176 4.58e1 SMART
LRR 198 223 4.09e1 SMART
LRR 224 247 1.33e1 SMART
LRR 248 269 4.2e0 SMART
LRR_TYP 270 293 7.9e-4 SMART
LRRCT 305 356 1.49e-9 SMART
FN3 404 486 4.56e0 SMART
transmembrane domain 530 552 N/A INTRINSIC
low complexity region 625 639 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000078027
SMART Domains Protein: ENSMUSP00000077174
Gene: ENSMUSG00000068205

DomainStartEndE-ValueType
low complexity region 6 20 N/A INTRINSIC
A1pp 71 201 5.72e-45 SMART
low complexity region 266 293 N/A INTRINSIC
low complexity region 307 319 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000110057
AA Change: T501S

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)
SMART Domains Protein: ENSMUSP00000105684
Gene: ENSMUSG00000051379
AA Change: T501S

DomainStartEndE-ValueType
LRRNT 30 62 3.12e-6 SMART
LRR 82 105 1.03e2 SMART
LRR 127 152 8.26e1 SMART
LRR 156 176 4.58e1 SMART
LRR 198 223 4.09e1 SMART
LRR 224 247 1.33e1 SMART
LRR 248 269 4.2e0 SMART
LRR_TYP 270 293 7.9e-4 SMART
LRRCT 305 356 1.49e-9 SMART
FN3 404 486 4.56e0 SMART
transmembrane domain 530 552 N/A INTRINSIC
low complexity region 625 639 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000110063
SMART Domains Protein: ENSMUSP00000105690
Gene: ENSMUSG00000068205

DomainStartEndE-ValueType
low complexity region 6 20 N/A INTRINSIC
PDB:4IQY|B 21 107 1e-36 PDB
Predicted Effect probably benign
Transcript: ENSMUST00000110064
SMART Domains Protein: ENSMUSP00000105691
Gene: ENSMUSG00000068205

DomainStartEndE-ValueType
low complexity region 6 20 N/A INTRINSIC
A1pp 71 201 5.72e-45 SMART
low complexity region 266 293 N/A INTRINSIC
low complexity region 307 319 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000110067
SMART Domains Protein: ENSMUSP00000105694
Gene: ENSMUSG00000068205

DomainStartEndE-ValueType
low complexity region 6 20 N/A INTRINSIC
A1pp 71 201 5.72e-45 SMART
Meta Mutation Damage Score 0.2609 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency 100% (74/74)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the fibronectin leucine rich transmembrane protein (FLRT) family. FLRTs may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. This gene is expressed in many tissues. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2010]
PHENOTYPE: Mice homozygous for a null allele exhibit embryonic lethality during organogenesis, abnormal embryonic tissue development, disrupted head formation, cardia bifida and abnormal ventral wall closure. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 76 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1700086D15Rik A T 11: 65,152,981 (GRCm38) F85I unknown Het
Ankrd22 A T 19: 34,149,365 (GRCm38) W22R possibly damaging Het
Aqr A T 2: 114,108,109 (GRCm38) H1287Q probably damaging Het
Armh1 C A 4: 117,213,741 (GRCm38) A396S probably benign Het
Atp8b5 A G 4: 43,366,609 (GRCm38) E791G probably damaging Het
Bicd1 T G 6: 149,513,726 (GRCm38) S646A possibly damaging Het
Bmpr1b A T 3: 141,856,599 (GRCm38) Y276N probably damaging Het
Cadps2 T C 6: 23,496,851 (GRCm38) H465R probably damaging Het
Card11 A T 5: 140,913,429 (GRCm38) probably null Het
Ccdc9 A G 7: 16,282,400 (GRCm38) L139P probably damaging Het
Cdk5rap3 A T 11: 96,909,945 (GRCm38) M355K probably benign Het
Cnot2 A T 10: 116,507,080 (GRCm38) V116E probably benign Het
Defb48 A G 14: 62,977,831 (GRCm38) V32A possibly damaging Het
Dmkn A G 7: 30,777,651 (GRCm38) D460G possibly damaging Het
Dmxl2 A G 9: 54,400,957 (GRCm38) V2170A possibly damaging Het
Dnah1 A T 14: 31,287,876 (GRCm38) F1912I possibly damaging Het
Dok3 C T 13: 55,527,493 (GRCm38) V71I probably benign Het
Dzip3 A T 16: 48,975,474 (GRCm38) F178Y probably damaging Het
Enpp1 T C 10: 24,674,410 (GRCm38) probably null Het
Eogt A G 6: 97,113,952 (GRCm38) F409L probably damaging Het
Erich6 A T 3: 58,630,689 (GRCm38) L218H probably damaging Het
Fam186a G C 15: 99,941,915 (GRCm38) I2149M possibly damaging Het
Fat1 G A 8: 45,023,427 (GRCm38) V1837I probably damaging Het
Fmn1 A C 2: 113,688,134 (GRCm38) E1365D probably damaging Het
Fzd5 G T 1: 64,736,092 (GRCm38) P170Q probably benign Het
Gm13101 A T 4: 143,965,817 (GRCm38) C205S probably benign Het
Gm14326 G A 2: 177,946,505 (GRCm38) H233Y probably damaging Het
Gm19410 G A 8: 35,790,612 (GRCm38) V735M probably damaging Het
Gm21886 ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG 18: 80,089,825 (GRCm38) probably benign Het
Gm2832 A T 14: 41,280,962 (GRCm38) I143L Het
Gm7534 T C 4: 134,200,073 (GRCm38) probably null Het
Greb1 G A 12: 16,716,765 (GRCm38) T344I probably benign Het
Grik2 A T 10: 49,523,822 (GRCm38) Y271N possibly damaging Het
Grin3b A G 10: 79,973,051 (GRCm38) N212D probably benign Het
Hgsnat A G 8: 25,971,049 (GRCm38) L187P probably damaging Het
Hmcn1 A G 1: 150,656,573 (GRCm38) I3152T probably damaging Het
Hsp90aa1 A T 12: 110,695,294 (GRCm38) I96N unknown Het
Il18r1 T A 1: 40,471,772 (GRCm38) L6I probably damaging Het
Ing3 G A 6: 21,953,799 (GRCm38) V80I probably damaging Het
Kif18b T C 11: 102,914,667 (GRCm38) I255V probably damaging Het
Kif2c A T 4: 117,182,432 (GRCm38) N20K possibly damaging Het
Mfsd6l T C 11: 68,558,038 (GRCm38) W572R probably damaging Het
Mybphl A G 3: 108,374,180 (GRCm38) T71A probably benign Het
Myo7a G T 7: 98,085,448 (GRCm38) T613K possibly damaging Het
Nfatc3 T C 8: 106,079,083 (GRCm38) S195P probably damaging Het
Nrg1 T C 8: 31,818,323 (GRCm38) N603S probably benign Het
Olfr1054 A T 2: 86,333,353 (GRCm38) M1K probably null Het
Olfr1084 A G 2: 86,639,669 (GRCm38) I13T possibly damaging Het
Olfr629 T C 7: 103,740,400 (GRCm38) Y280C probably damaging Het
Parp8 T C 13: 116,894,805 (GRCm38) E457G probably damaging Het
Pclo T C 5: 14,521,062 (GRCm38) F154L probably benign Het
Pear1 A G 3: 87,752,568 (GRCm38) S704P probably damaging Het
Pkib A G 10: 57,736,298 (GRCm38) T92A probably benign Het
Pnpla7 A G 2: 24,998,666 (GRCm38) R376G possibly damaging Het
Ptprd T A 4: 76,066,327 (GRCm38) E527D probably benign Het
Pum1 T C 4: 130,747,026 (GRCm38) V469A probably damaging Het
Rnf148 G A 6: 23,654,284 (GRCm38) Q238* probably null Het
Scn9a A C 2: 66,483,646 (GRCm38) N1909K probably benign Het
Sema3c A T 5: 17,727,596 (GRCm38) H699L possibly damaging Het
Sema3f A T 9: 107,689,728 (GRCm38) C201S probably damaging Het
Serinc2 T A 4: 130,258,790 (GRCm38) D206V probably benign Het
Serping1 A T 2: 84,767,293 (GRCm38) S415T probably benign Het
Sirpb1b A T 3: 15,548,872 (GRCm38) L50Q probably damaging Het
Slc25a22 T C 7: 141,431,383 (GRCm38) E262G probably benign Het
Slc5a8 A G 10: 88,902,491 (GRCm38) I205M probably damaging Het
Sod2 G T 17: 13,008,031 (GRCm38) probably benign Het
Tec T C 5: 72,786,019 (GRCm38) I118V probably benign Het
Tenm3 A T 8: 48,287,812 (GRCm38) V1212E probably damaging Het
Tex44 G A 1: 86,426,515 (GRCm38) V49I probably benign Het
Tmem132d A T 5: 127,784,141 (GRCm38) L972* probably null Het
Tpte G A 8: 22,325,547 (GRCm38) probably null Het
Trim6 T A 7: 104,232,832 (GRCm38) I456N probably damaging Het
Ubr4 G C 4: 139,422,417 (GRCm38) V520L probably benign Het
Ubxn8 A T 8: 33,633,607 (GRCm38) N101K probably benign Het
Vmn2r60 AG A 7: 42,195,734 (GRCm38) probably null Het
Vmn2r83 A G 10: 79,491,558 (GRCm38) T667A probably damaging Het
Other mutations in Flrt3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02141:Flrt3 APN 2 140,660,688 (GRCm38) missense probably damaging 0.99
IGL02292:Flrt3 APN 2 140,660,125 (GRCm38) missense probably damaging 1.00
R0030:Flrt3 UTSW 2 140,660,317 (GRCm38) missense probably damaging 1.00
R1487:Flrt3 UTSW 2 140,660,934 (GRCm38) missense probably damaging 1.00
R2061:Flrt3 UTSW 2 140,661,453 (GRCm38) missense probably damaging 1.00
R2115:Flrt3 UTSW 2 140,661,503 (GRCm38) missense probably damaging 1.00
R2326:Flrt3 UTSW 2 140,661,391 (GRCm38) missense possibly damaging 0.50
R3113:Flrt3 UTSW 2 140,661,534 (GRCm38) missense probably benign 0.03
R3605:Flrt3 UTSW 2 140,661,367 (GRCm38) missense probably damaging 1.00
R4366:Flrt3 UTSW 2 140,660,407 (GRCm38) missense probably damaging 0.99
R4702:Flrt3 UTSW 2 140,661,655 (GRCm38) missense probably benign
R4799:Flrt3 UTSW 2 140,660,166 (GRCm38) missense probably damaging 1.00
R5085:Flrt3 UTSW 2 140,660,257 (GRCm38) missense probably damaging 0.99
R5100:Flrt3 UTSW 2 140,671,384 (GRCm38) start gained probably null
R5109:Flrt3 UTSW 2 140,660,743 (GRCm38) missense possibly damaging 0.51
R5635:Flrt3 UTSW 2 140,660,500 (GRCm38) missense probably damaging 1.00
R5982:Flrt3 UTSW 2 140,660,916 (GRCm38) missense possibly damaging 0.95
R6117:Flrt3 UTSW 2 140,660,445 (GRCm38) missense possibly damaging 0.46
R6213:Flrt3 UTSW 2 140,661,165 (GRCm38) missense probably damaging 1.00
R6246:Flrt3 UTSW 2 140,659,801 (GRCm38) missense probably damaging 1.00
R6746:Flrt3 UTSW 2 140,660,025 (GRCm38) missense probably damaging 0.99
R6854:Flrt3 UTSW 2 140,660,718 (GRCm38) missense probably damaging 1.00
R7000:Flrt3 UTSW 2 140,660,884 (GRCm38) nonsense probably null
R7221:Flrt3 UTSW 2 140,661,170 (GRCm38) missense probably damaging 0.99
R7388:Flrt3 UTSW 2 140,661,752 (GRCm38) critical splice acceptor site probably null
R7444:Flrt3 UTSW 2 140,660,467 (GRCm38) missense probably benign 0.00
R7967:Flrt3 UTSW 2 140,659,891 (GRCm38) nonsense probably null
R8272:Flrt3 UTSW 2 140,660,697 (GRCm38) missense probably damaging 1.00
R8441:Flrt3 UTSW 2 140,660,626 (GRCm38) missense probably benign 0.00
R9109:Flrt3 UTSW 2 140,659,959 (GRCm38) missense probably damaging 1.00
R9257:Flrt3 UTSW 2 140,660,239 (GRCm38) missense probably benign
R9298:Flrt3 UTSW 2 140,659,959 (GRCm38) missense probably damaging 1.00
R9482:Flrt3 UTSW 2 140,661,670 (GRCm38) missense probably benign
R9629:Flrt3 UTSW 2 140,660,896 (GRCm38) missense possibly damaging 0.53
Predicted Primers PCR Primer
(F):5'- TACGCACAGTTCCGTGAAAAC -3'
(R):5'- CTATGACTGCTCTGCGACTCAG -3'

Sequencing Primer
(F):5'- GTGAAAACAGTGACCCGTTCCTATG -3'
(R):5'- ACTCAGCTGGCTTAAACTGG -3'
Posted On 2019-10-17