Mutagenetix > Incidental Mutation

Incidental Mutation 'R7526:Tmem132d'

582983

Institutional Source

Beutler Lab

Gene Symbol

Tmem132d

Ensembl Gene

ENSMUSG00000034310

Gene Name

transmembrane protein 132D

Synonyms

MMRRC Submission

045598-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.166) question?

Stock #

R7526 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

127781630-128433077 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 127784141 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Stop codon at position 972 (L972*)

Ref Sequence

ENSEMBL: ENSMUSP00000043633 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044441]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000044441
AA Change: L972*

SMART Domains

Protein: ENSMUSP00000043633
Gene: ENSMUSG00000034310
AA Change: L972*

Domain	Start	End	E-Value	Type
signal peptide	1	30	N/A	INTRINSIC
Pfam:TMEM132D_N	49	178	1.9e-59	PFAM
Pfam:TMEM132	435	778	3.9e-150	PFAM
Pfam:TMEM132D_C	884	970	1.9e-37	PFAM
low complexity region	998	1011	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (74/74)

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700086D15Rik	A	T	11: 65,152,981	F85I	unknown	Het
Ankrd22	A	T	19: 34,149,365	W22R	possibly damaging	Het
Aqr	A	T	2: 114,108,109	H1287Q	probably damaging	Het
Armh1	C	A	4: 117,213,741	A396S	probably benign	Het
Atp8b5	A	G	4: 43,366,609	E791G	probably damaging	Het
Bicd1	T	G	6: 149,513,726	S646A	possibly damaging	Het
Bmpr1b	A	T	3: 141,856,599	Y276N	probably damaging	Het
Cadps2	T	C	6: 23,496,851	H465R	probably damaging	Het
Card11	A	T	5: 140,913,429		probably null	Het
Ccdc9	A	G	7: 16,282,400	L139P	probably damaging	Het
Cdk5rap3	A	T	11: 96,909,945	M355K	probably benign	Het
Cnot2	A	T	10: 116,507,080	V116E	probably benign	Het
Defb48	A	G	14: 62,977,831	V32A	possibly damaging	Het
Dmkn	A	G	7: 30,777,651	D460G	possibly damaging	Het
Dmxl2	A	G	9: 54,400,957	V2170A	possibly damaging	Het
Dnah1	A	T	14: 31,287,876	F1912I	possibly damaging	Het
Dok3	C	T	13: 55,527,493	V71I	probably benign	Het
Dzip3	A	T	16: 48,975,474	F178Y	probably damaging	Het
Enpp1	T	C	10: 24,674,410		probably null	Het
Eogt	A	G	6: 97,113,952	F409L	probably damaging	Het
Erich6	A	T	3: 58,630,689	L218H	probably damaging	Het
Fam186a	G	C	15: 99,941,915	I2149M	possibly damaging	Het
Fat1	G	A	8: 45,023,427	V1837I	probably damaging	Het
Flrt3	T	A	2: 140,660,206	T501S	probably damaging	Het
Fmn1	A	C	2: 113,688,134	E1365D	probably damaging	Het
Fzd5	G	T	1: 64,736,092	P170Q	probably benign	Het
Gm13101	A	T	4: 143,965,817	C205S	probably benign	Het
Gm14326	G	A	2: 177,946,505	H233Y	probably damaging	Het
Gm19410	G	A	8: 35,790,612	V735M	probably damaging	Het
Gm21886	ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG	ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG	18: 80,089,825		probably benign	Het
Gm2832	A	T	14: 41,280,962	I143L		Het
Gm7534	T	C	4: 134,200,073		probably null	Het
Greb1	G	A	12: 16,716,765	T344I	probably benign	Het
Grik2	A	T	10: 49,523,822	Y271N	possibly damaging	Het
Grin3b	A	G	10: 79,973,051	N212D	probably benign	Het
Hgsnat	A	G	8: 25,971,049	L187P	probably damaging	Het
Hmcn1	A	G	1: 150,656,573	I3152T	probably damaging	Het
Hsp90aa1	A	T	12: 110,695,294	I96N	unknown	Het
Il18r1	T	A	1: 40,471,772	L6I	probably damaging	Het
Ing3	G	A	6: 21,953,799	V80I	probably damaging	Het
Kif18b	T	C	11: 102,914,667	I255V	probably damaging	Het
Kif2c	A	T	4: 117,182,432	N20K	possibly damaging	Het
Mfsd6l	T	C	11: 68,558,038	W572R	probably damaging	Het
Mybphl	A	G	3: 108,374,180	T71A	probably benign	Het
Myo7a	G	T	7: 98,085,448	T613K	possibly damaging	Het
Nfatc3	T	C	8: 106,079,083	S195P	probably damaging	Het
Nrg1	T	C	8: 31,818,323	N603S	probably benign	Het
Olfr1054	A	T	2: 86,333,353	M1K	probably null	Het
Olfr1084	A	G	2: 86,639,669	I13T	possibly damaging	Het
Olfr629	T	C	7: 103,740,400	Y280C	probably damaging	Het
Parp8	T	C	13: 116,894,805	E457G	probably damaging	Het
Pclo	T	C	5: 14,521,062	F154L	probably benign	Het
Pear1	A	G	3: 87,752,568	S704P	probably damaging	Het
Pkib	A	G	10: 57,736,298	T92A	probably benign	Het
Pnpla7	A	G	2: 24,998,666	R376G	possibly damaging	Het
Ptprd	T	A	4: 76,066,327	E527D	probably benign	Het
Pum1	T	C	4: 130,747,026	V469A	probably damaging	Het
Rnf148	G	A	6: 23,654,284	Q238*	probably null	Het
Scn9a	A	C	2: 66,483,646	N1909K	probably benign	Het
Sema3c	A	T	5: 17,727,596	H699L	possibly damaging	Het
Sema3f	A	T	9: 107,689,728	C201S	probably damaging	Het
Serinc2	T	A	4: 130,258,790	D206V	probably benign	Het
Serping1	A	T	2: 84,767,293	S415T	probably benign	Het
Sirpb1b	A	T	3: 15,548,872	L50Q	probably damaging	Het
Slc25a22	T	C	7: 141,431,383	E262G	probably benign	Het
Slc5a8	A	G	10: 88,902,491	I205M	probably damaging	Het
Sod2	G	T	17: 13,008,031		probably benign	Het
Tec	T	C	5: 72,786,019	I118V	probably benign	Het
Tenm3	A	T	8: 48,287,812	V1212E	probably damaging	Het
Tex44	G	A	1: 86,426,515	V49I	probably benign	Het
Tpte	G	A	8: 22,325,547		probably null	Het
Trim6	T	A	7: 104,232,832	I456N	probably damaging	Het
Ubr4	G	C	4: 139,422,417	V520L	probably benign	Het
Ubxn8	A	T	8: 33,633,607	N101K	probably benign	Het
Vmn2r60	AG	A	7: 42,195,734		probably null	Het
Vmn2r83	A	G	10: 79,491,558	T667A	probably damaging	Het

Other mutations in Tmem132d

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00990:Tmem132d	APN	5	127784832	missense	possibly damaging	0.77
IGL01393:Tmem132d	APN	5	127784638	missense	probably benign	0.31
IGL01482:Tmem132d	APN	5	128269206	missense	probably damaging	0.96
IGL01785:Tmem132d	APN	5	127984315	missense	probably benign	0.00
IGL02409:Tmem132d	APN	5	127784888	missense	probably damaging	1.00
IGL02539:Tmem132d	APN	5	127783979	missense	probably benign	0.01
IGL03411:Tmem132d	APN	5	127984283	nonsense	probably null
R0113:Tmem132d	UTSW	5	127784593	missense	probably benign	0.11
R0420:Tmem132d	UTSW	5	127864646	missense	probably benign	0.26
R0437:Tmem132d	UTSW	5	127789785	missense	probably damaging	0.99
R0468:Tmem132d	UTSW	5	128269203	missense	probably damaging	1.00
R0564:Tmem132d	UTSW	5	127784778	missense	probably damaging	1.00
R0659:Tmem132d	UTSW	5	127984287	missense	possibly damaging	0.94
R0924:Tmem132d	UTSW	5	127984439	splice site	probably benign
R1209:Tmem132d	UTSW	5	127784870	missense	probably damaging	1.00
R1333:Tmem132d	UTSW	5	127784859	missense	probably benign
R1378:Tmem132d	UTSW	5	128268947	missense	probably benign	0.43
R1741:Tmem132d	UTSW	5	127784858	missense	probably benign	0.30
R1753:Tmem132d	UTSW	5	127789855	missense	probably benign	0.02
R1944:Tmem132d	UTSW	5	127783764	makesense	probably null
R1974:Tmem132d	UTSW	5	128269199	missense	probably damaging	0.99
R2035:Tmem132d	UTSW	5	127792458	missense	probably damaging	1.00
R2065:Tmem132d	UTSW	5	127784441	missense	probably benign
R2074:Tmem132d	UTSW	5	128269131	missense	probably damaging	1.00
R2276:Tmem132d	UTSW	5	127795923	missense	probably damaging	1.00
R2297:Tmem132d	UTSW	5	128268544	missense	possibly damaging	0.69
R2424:Tmem132d	UTSW	5	127864599	missense	probably benign	0.09
R2902:Tmem132d	UTSW	5	127783768	missense	probably benign
R3053:Tmem132d	UTSW	5	127792474	missense	probably benign	0.15
R3836:Tmem132d	UTSW	5	127784885	missense	probably damaging	1.00
R4127:Tmem132d	UTSW	5	128268820	missense	probably benign	0.35
R4236:Tmem132d	UTSW	5	128432325	missense	possibly damaging	0.89
R4358:Tmem132d	UTSW	5	127984341	missense	possibly damaging	0.92
R4610:Tmem132d	UTSW	5	127984296	missense	probably benign	0.29
R4686:Tmem132d	UTSW	5	127792610	missense	possibly damaging	0.55
R4814:Tmem132d	UTSW	5	127984264	missense	probably benign	0.01
R4883:Tmem132d	UTSW	5	128269300	missense	probably damaging	0.99
R4883:Tmem132d	UTSW	5	128269302	missense	possibly damaging	0.79
R4939:Tmem132d	UTSW	5	127796075	missense	probably damaging	1.00
R5579:Tmem132d	UTSW	5	127796000	missense	possibly damaging	0.67
R5652:Tmem132d	UTSW	5	127784795	missense	possibly damaging	0.88
R5801:Tmem132d	UTSW	5	127784900	missense	possibly damaging	0.50
R5900:Tmem132d	UTSW	5	128269272	missense	probably damaging	1.00
R5980:Tmem132d	UTSW	5	127784598	missense	probably benign	0.13
R6048:Tmem132d	UTSW	5	128269117	missense	probably benign	0.03
R6057:Tmem132d	UTSW	5	127784870	missense	probably damaging	1.00
R6084:Tmem132d	UTSW	5	127784100	missense	probably benign	0.06
R6505:Tmem132d	UTSW	5	127784438	missense	probably benign	0.00
R6522:Tmem132d	UTSW	5	127783768	missense	probably benign
R6540:Tmem132d	UTSW	5	128268532	missense	possibly damaging	0.87
R6717:Tmem132d	UTSW	5	127784421	missense	probably benign
R7158:Tmem132d	UTSW	5	128137019	missense	possibly damaging	0.81
R7287:Tmem132d	UTSW	5	127984351	missense	probably damaging	0.96
R7826:Tmem132d	UTSW	5	127789889	missense	probably damaging	1.00
R7864:Tmem132d	UTSW	5	127783916	missense	probably damaging	1.00
R8124:Tmem132d	UTSW	5	127792560	missense	probably damaging	1.00
R8543:Tmem132d	UTSW	5	128268735	missense	probably benign	0.00
R8694:Tmem132d	UTSW	5	127792431	missense	probably benign	0.06
R8936:Tmem132d	UTSW	5	127792612	missense	probably damaging	1.00
R9017:Tmem132d	UTSW	5	127789872	missense	probably benign	0.00
R9017:Tmem132d	UTSW	5	128269252	missense	probably benign	0.00
R9163:Tmem132d	UTSW	5	127792506	missense	possibly damaging	0.82
R9257:Tmem132d	UTSW	5	127784427	nonsense	probably null
R9645:Tmem132d	UTSW	5	128269011	missense	probably damaging	1.00
R9667:Tmem132d	UTSW	5	127984311	missense	possibly damaging	0.79
R9711:Tmem132d	UTSW	5	127792515	missense	possibly damaging	0.94

Predicted Primers

PCR Primer
(F):5'- ACCCATCGTCTGAGGAGATG -3'
(R):5'- GTGACCTCGAAATTGGCATG -3'

Sequencing Primer
(F):5'- CCGAGAATGTGGAAAATGTCACTC -3'
(R):5'- CATGTATGCTCTGCTGGGTGTC -3'

Posted On

2019-10-17