Mutagenetix > Incidental Mutation

Incidental Mutation 'R7526:Fat1'

583001

Institutional Source

Beutler Lab

Gene Symbol

Fat1

Ensembl Gene

ENSMUSG00000070047

Gene Name

FAT atypical cadherin 1

Synonyms

2310038E12Rik, mFat1, Fath

MMRRC Submission

045598-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7526 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

45388484-45505294 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 45476464 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 1837 (V1837I)

Ref Sequence

ENSEMBL: ENSMUSP00000149194 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000098796] [ENSMUST00000189017] [ENSMUST00000191428] [ENSMUST00000215588]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000098796
AA Change: V1814I

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000096394
Gene: ENSMUSG00000070047
AA Change: V1814I

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000189017
AA Change: V1814I

Predicted Effect

probably damaging
Transcript: ENSMUST00000191428
AA Change: V1814I

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000140596
Gene: ENSMUSG00000070047
AA Change: V1814I

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
CA	62	148	3.05e-6	SMART
CA	172	256	3.29e-20	SMART
Blast:CA	277	382	5e-47	BLAST
CA	387	462	2.13e-5	SMART
CA	486	568	8.35e-22	SMART
CA	592	670	2.11e-2	SMART
CA	740	821	5.09e-26	SMART
CA	845	926	6.27e-26	SMART
CA	950	1031	4.07e-25	SMART
CA	1057	1138	5.13e-31	SMART
CA	1162	1244	8.79e-30	SMART
CA	1276	1351	2.06e-3	SMART
CA	1379	1456	1.63e-15	SMART
CA	1480	1562	3.29e-20	SMART
CA	1586	1667	2.34e-16	SMART
CA	1691	1765	1.16e-20	SMART
CA	1796	1879	6.27e-26	SMART
CA	1903	1979	1.47e-8	SMART
CA	2003	2081	2.65e-15	SMART
CA	2105	2181	2.14e-10	SMART
CA	2203	2283	9.82e-19	SMART
CA	2307	2390	7.54e-29	SMART
CA	2414	2492	3.29e-11	SMART
CA	2516	2596	6.48e-19	SMART
CA	2620	2703	3.48e-10	SMART
CA	2719	2809	2.26e-9	SMART
CA	2833	2918	8.08e-29	SMART
CA	2942	3023	5.99e-23	SMART
CA	3047	3125	2.63e-28	SMART
CA	3149	3230	2.79e-32	SMART
CA	3254	3335	5.25e-28	SMART
CA	3359	3440	4.46e-31	SMART
CA	3464	3545	1.25e-11	SMART
CA	3569	3641	5.67e-2	SMART
LamG	3853	3987	6.51e-36	SMART
EGF	4018	4052	8.57e-5	SMART
EGF	4057	4090	3.94e-4	SMART
EGF	4094	4127	4.29e-5	SMART
EGF_CA	4129	4165	1.81e-12	SMART
transmembrane domain	4182	4204	N/A	INTRINSIC
low complexity region	4308	4324	N/A	INTRINSIC
low complexity region	4436	4457	N/A	INTRINSIC
low complexity region	4472	4483	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000215588
AA Change: V1837I

PolyPhen 2 Score 0.995 (Sensitivity: 0.68; Specificity: 0.97)

Meta Mutation Damage Score

0.0969

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (74/74)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. In addition to containing 34 tandem cadherin-type repeats, the gene product has five epidermal growth factor (EGF)-like repeats and one laminin A-G domain. This gene is expressed at high levels in a number of fetal epithelia. Its product probably functions as an adhesion molecule and/or signaling receptor, and is likely to be important in developmental processes and cell communication. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit holoprosencephaly, anophthalmia, kidney defects and perinatal lethality. Mice homozygous for a hypomorphic allele exhibit altered shoulder girdle and facial musculature, retinal defects, abnormal inner earpatterning and kidney defects. [provided by MGI curators]

Allele List at MGI

All alleles(56) : Targeted, other(1) Gene trapped(55)

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700086D15Rik	A	T	11: 65,043,807 (GRCm39)	F85I	unknown	Het
Ankrd22	A	T	19: 34,126,765 (GRCm39)	W22R	possibly damaging	Het
Aqr	A	T	2: 113,938,590 (GRCm39)	H1287Q	probably damaging	Het
Armh1	C	A	4: 117,070,938 (GRCm39)	A396S	probably benign	Het
Atp8b5	A	G	4: 43,366,609 (GRCm39)	E791G	probably damaging	Het
Bicd1	T	G	6: 149,415,224 (GRCm39)	S646A	possibly damaging	Het
Bmpr1b	A	T	3: 141,562,360 (GRCm39)	Y276N	probably damaging	Het
Cadps2	T	C	6: 23,496,850 (GRCm39)	H465R	probably damaging	Het
Card11	A	T	5: 140,899,184 (GRCm39)		probably null	Het
Ccdc9	A	G	7: 16,016,325 (GRCm39)	L139P	probably damaging	Het
Cdk5rap3	A	T	11: 96,800,771 (GRCm39)	M355K	probably benign	Het
Cnot2	A	T	10: 116,342,985 (GRCm39)	V116E	probably benign	Het
Defb48	A	G	14: 63,215,280 (GRCm39)	V32A	possibly damaging	Het
Dmkn	A	G	7: 30,477,076 (GRCm39)	D460G	possibly damaging	Het
Dmxl2	A	G	9: 54,308,241 (GRCm39)	V2170A	possibly damaging	Het
Dnah1	A	T	14: 31,009,833 (GRCm39)	F1912I	possibly damaging	Het
Dok3	C	T	13: 55,675,306 (GRCm39)	V71I	probably benign	Het
Dzip3	A	T	16: 48,795,837 (GRCm39)	F178Y	probably damaging	Het
Enpp1	T	C	10: 24,550,308 (GRCm39)		probably null	Het
Eogt	A	G	6: 97,090,913 (GRCm39)	F409L	probably damaging	Het
Erich6	A	T	3: 58,538,110 (GRCm39)	L218H	probably damaging	Het
Fam186a	G	C	15: 99,839,796 (GRCm39)	I2149M	possibly damaging	Het
Flrt3	T	A	2: 140,502,126 (GRCm39)	T501S	probably damaging	Het
Fmn1	A	C	2: 113,518,479 (GRCm39)	E1365D	probably damaging	Het
Fzd5	G	T	1: 64,775,251 (GRCm39)	P170Q	probably benign	Het
Gm14326	G	A	2: 177,588,298 (GRCm39)	H233Y	probably damaging	Het
Gm19410	G	A	8: 36,257,766 (GRCm39)	V735M	probably damaging	Het
Gm21886	ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG	ACTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGGCCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGACCTGCAGACAGTAGGTGCTCACTGAGG	18: 80,133,040 (GRCm39)		probably benign	Het
Gm2832	A	T	14: 41,002,919 (GRCm39)	I143L		Het
Greb1	G	A	12: 16,766,766 (GRCm39)	T344I	probably benign	Het
Grik2	A	T	10: 49,399,918 (GRCm39)	Y271N	possibly damaging	Het
Grin3b	A	G	10: 79,808,885 (GRCm39)	N212D	probably benign	Het
Hgsnat	A	G	8: 26,461,077 (GRCm39)	L187P	probably damaging	Het
Hmcn1	A	G	1: 150,532,324 (GRCm39)	I3152T	probably damaging	Het
Hsp90aa1	A	T	12: 110,661,728 (GRCm39)	I96N	unknown	Het
Il18r1	T	A	1: 40,510,932 (GRCm39)	L6I	probably damaging	Het
Ing3	G	A	6: 21,953,798 (GRCm39)	V80I	probably damaging	Het
Kif18b	T	C	11: 102,805,493 (GRCm39)	I255V	probably damaging	Het
Kif2c	A	T	4: 117,039,629 (GRCm39)	N20K	possibly damaging	Het
Mfsd6l	T	C	11: 68,448,864 (GRCm39)	W572R	probably damaging	Het
Mybphl	A	G	3: 108,281,496 (GRCm39)	T71A	probably benign	Het
Myo7a	G	T	7: 97,734,655 (GRCm39)	T613K	possibly damaging	Het
Nfatc3	T	C	8: 106,805,715 (GRCm39)	S195P	probably damaging	Het
Nrg1	T	C	8: 32,308,351 (GRCm39)	N603S	probably benign	Het
Or52ae9	T	C	7: 103,389,607 (GRCm39)	Y280C	probably damaging	Het
Or8k22	A	T	2: 86,163,697 (GRCm39)	M1K	probably null	Het
Or8k37	A	G	2: 86,470,013 (GRCm39)	I13T	possibly damaging	Het
Parp8	T	C	13: 117,031,341 (GRCm39)	E457G	probably damaging	Het
Pclo	T	C	5: 14,571,076 (GRCm39)	F154L	probably benign	Het
Pear1	A	G	3: 87,659,875 (GRCm39)	S704P	probably damaging	Het
Pkib	A	G	10: 57,612,394 (GRCm39)	T92A	probably benign	Het
Pnpla7	A	G	2: 24,888,678 (GRCm39)	R376G	possibly damaging	Het
Pramel28	A	T	4: 143,692,387 (GRCm39)	C205S	probably benign	Het
Ptprd	T	A	4: 75,984,564 (GRCm39)	E527D	probably benign	Het
Pum1	T	C	4: 130,474,337 (GRCm39)	V469A	probably damaging	Het
Rnf148	G	A	6: 23,654,283 (GRCm39)	Q238*	probably null	Het
Scn9a	A	C	2: 66,313,990 (GRCm39)	N1909K	probably benign	Het
Sema3c	A	T	5: 17,932,594 (GRCm39)	H699L	possibly damaging	Het
Sema3f	A	T	9: 107,566,927 (GRCm39)	C201S	probably damaging	Het
Serinc2	T	A	4: 130,152,583 (GRCm39)	D206V	probably benign	Het
Serping1	A	T	2: 84,597,637 (GRCm39)	S415T	probably benign	Het
Sirpb1b	A	T	3: 15,613,932 (GRCm39)	L50Q	probably damaging	Het
Slc25a22	T	C	7: 141,011,296 (GRCm39)	E262G	probably benign	Het
Slc5a8	A	G	10: 88,738,353 (GRCm39)	I205M	probably damaging	Het
Sod2	G	T	17: 13,226,918 (GRCm39)		probably benign	Het
Tec	T	C	5: 72,943,362 (GRCm39)	I118V	probably benign	Het
Tenm3	A	T	8: 48,740,847 (GRCm39)	V1212E	probably damaging	Het
Tex44	G	A	1: 86,354,237 (GRCm39)	V49I	probably benign	Het
Tmem132d	A	T	5: 127,861,205 (GRCm39)	L972*	probably null	Het
Tpte	G	A	8: 22,815,563 (GRCm39)		probably null	Het
Trim6	T	A	7: 103,882,039 (GRCm39)	I456N	probably damaging	Het
Ubr4	G	C	4: 139,149,728 (GRCm39)	V520L	probably benign	Het
Ubxn8	A	T	8: 34,123,635 (GRCm39)	N101K	probably benign	Het
Vmn2r60	AG	A	7: 41,845,158 (GRCm39)		probably null	Het
Vmn2r83	A	G	10: 79,327,392 (GRCm39)	T667A	probably damaging	Het
Zpld2	T	C	4: 133,927,384 (GRCm39)		probably null	Het

Other mutations in Fat1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00088:Fat1	APN	8	45,477,639 (GRCm39)	missense	possibly damaging	0.93
IGL00157:Fat1	APN	8	45,404,707 (GRCm39)	missense	possibly damaging	0.96
IGL00481:Fat1	APN	8	45,503,977 (GRCm39)	missense	probably benign	0.18
IGL00983:Fat1	APN	8	45,486,427 (GRCm39)	missense	probably damaging	1.00
IGL01089:Fat1	APN	8	45,470,894 (GRCm39)	missense	probably damaging	1.00
IGL01135:Fat1	APN	8	45,477,877 (GRCm39)	missense	probably damaging	1.00
IGL01143:Fat1	APN	8	45,488,569 (GRCm39)	missense	possibly damaging	0.72
IGL01155:Fat1	APN	8	45,476,986 (GRCm39)	missense	probably damaging	1.00
IGL01376:Fat1	APN	8	45,479,878 (GRCm39)	missense	probably benign	0.00
IGL01411:Fat1	APN	8	45,479,837 (GRCm39)	missense	probably damaging	1.00
IGL01443:Fat1	APN	8	45,493,613 (GRCm39)	missense	probably damaging	1.00
IGL01453:Fat1	APN	8	45,504,307 (GRCm39)	missense	probably damaging	1.00
IGL01606:Fat1	APN	8	45,476,086 (GRCm39)	missense	probably benign	0.26
IGL01622:Fat1	APN	8	45,482,592 (GRCm39)	missense	possibly damaging	0.64
IGL01623:Fat1	APN	8	45,482,592 (GRCm39)	missense	possibly damaging	0.64
IGL01672:Fat1	APN	8	45,493,737 (GRCm39)	missense	probably benign	0.05
IGL01735:Fat1	APN	8	45,489,276 (GRCm39)	missense	probably benign	0.07
IGL01793:Fat1	APN	8	45,442,149 (GRCm39)	missense	probably benign
IGL01820:Fat1	APN	8	45,463,539 (GRCm39)	missense	probably damaging	1.00
IGL01969:Fat1	APN	8	45,405,636 (GRCm39)	missense	probably damaging	0.98
IGL02012:Fat1	APN	8	45,480,577 (GRCm39)	missense	possibly damaging	0.95
IGL02227:Fat1	APN	8	45,476,696 (GRCm39)	missense	probably damaging	1.00
IGL02256:Fat1	APN	8	45,403,369 (GRCm39)	missense	probably damaging	1.00
IGL02273:Fat1	APN	8	45,403,368 (GRCm39)	missense	probably damaging	1.00
IGL02317:Fat1	APN	8	45,478,855 (GRCm39)	missense	probably benign	0.33
IGL02324:Fat1	APN	8	45,493,593 (GRCm39)	missense	probably damaging	1.00
IGL02336:Fat1	APN	8	45,404,620 (GRCm39)	missense	probably benign	0.16
IGL02442:Fat1	APN	8	45,403,360 (GRCm39)	missense	probably benign	0.02
IGL02486:Fat1	APN	8	45,478,109 (GRCm39)	missense	probably benign	0.16
IGL02551:Fat1	APN	8	45,504,435 (GRCm39)	missense	probably damaging	1.00
IGL02617:Fat1	APN	8	45,488,628 (GRCm39)	missense	probably benign	0.31
IGL02698:Fat1	APN	8	45,476,201 (GRCm39)	missense	probably benign
IGL02885:Fat1	APN	8	45,442,204 (GRCm39)	missense	probably benign	0.01
IGL02904:Fat1	APN	8	45,493,719 (GRCm39)	missense	probably damaging	1.00
IGL02953:Fat1	APN	8	45,477,351 (GRCm39)	missense	probably damaging	1.00
IGL03108:Fat1	APN	8	45,476,651 (GRCm39)	missense	probably damaging	1.00
IGL03153:Fat1	APN	8	45,483,160 (GRCm39)	missense	possibly damaging	0.83
IGL03183:Fat1	APN	8	45,403,623 (GRCm39)	missense	probably damaging	0.99
IGL03327:Fat1	APN	8	45,403,505 (GRCm39)	missense	probably damaging	1.00
IGL03405:Fat1	APN	8	45,478,278 (GRCm39)	missense	probably damaging	1.00
Laggardly	UTSW	8	45,497,501 (GRCm39)	missense	probably damaging	1.00
R2257_fat1_465	UTSW	8	45,403,408 (GRCm39)	missense	probably damaging	1.00
Shrinkage	UTSW	8	45,471,074 (GRCm39)	missense	probably damaging	1.00
F5493:Fat1	UTSW	8	45,478,517 (GRCm39)	missense	probably damaging	0.99
G1citation:Fat1	UTSW	8	45,479,441 (GRCm39)	missense	probably damaging	1.00
I2289:Fat1	UTSW	8	45,478,033 (GRCm39)	missense	probably benign	0.01
IGL02837:Fat1	UTSW	8	45,470,471 (GRCm39)	missense	probably benign	0.00
PIT4283001:Fat1	UTSW	8	45,490,244 (GRCm39)	missense	probably damaging	1.00
PIT4283001:Fat1	UTSW	8	45,482,577 (GRCm39)	missense	probably damaging	1.00
PIT4576001:Fat1	UTSW	8	45,477,682 (GRCm39)	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45,479,441 (GRCm39)	missense	probably damaging	1.00
R0040:Fat1	UTSW	8	45,479,441 (GRCm39)	missense	probably damaging	1.00
R0078:Fat1	UTSW	8	45,406,336 (GRCm39)	missense	probably damaging	1.00
R0197:Fat1	UTSW	8	45,479,590 (GRCm39)	missense	probably benign	0.00
R0328:Fat1	UTSW	8	45,476,827 (GRCm39)	missense	probably benign	0.35
R0367:Fat1	UTSW	8	45,477,350 (GRCm39)	missense	probably damaging	1.00
R0371:Fat1	UTSW	8	45,404,929 (GRCm39)	missense	probably damaging	1.00
R0380:Fat1	UTSW	8	45,463,160 (GRCm39)	missense	probably damaging	0.97
R0389:Fat1	UTSW	8	45,403,385 (GRCm39)	missense	probably benign	0.00
R0433:Fat1	UTSW	8	45,477,686 (GRCm39)	missense	possibly damaging	0.51
R0456:Fat1	UTSW	8	45,482,571 (GRCm39)	missense	probably damaging	1.00
R0494:Fat1	UTSW	8	45,403,579 (GRCm39)	missense	probably damaging	1.00
R0506:Fat1	UTSW	8	45,475,988 (GRCm39)	missense	probably damaging	0.99
R0512:Fat1	UTSW	8	45,404,369 (GRCm39)	nonsense	probably null
R0624:Fat1	UTSW	8	45,504,205 (GRCm39)	missense	possibly damaging	0.46
R0701:Fat1	UTSW	8	45,479,590 (GRCm39)	missense	probably benign	0.00
R0723:Fat1	UTSW	8	45,479,786 (GRCm39)	missense	probably damaging	1.00
R0787:Fat1	UTSW	8	45,493,592 (GRCm39)	missense	probably damaging	1.00
R0788:Fat1	UTSW	8	45,477,020 (GRCm39)	missense	probably benign	0.27
R0862:Fat1	UTSW	8	45,471,074 (GRCm39)	missense	probably damaging	1.00
R0864:Fat1	UTSW	8	45,471,074 (GRCm39)	missense	probably damaging	1.00
R0907:Fat1	UTSW	8	45,479,635 (GRCm39)	missense	probably benign	0.08
R0962:Fat1	UTSW	8	45,486,363 (GRCm39)	splice site	probably benign
R1051:Fat1	UTSW	8	45,497,543 (GRCm39)	missense	probably damaging	1.00
R1156:Fat1	UTSW	8	45,492,927 (GRCm39)	missense	possibly damaging	0.94
R1237:Fat1	UTSW	8	45,497,316 (GRCm39)	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45,463,582 (GRCm39)	missense	probably damaging	1.00
R1468:Fat1	UTSW	8	45,463,582 (GRCm39)	missense	probably damaging	1.00
R1478:Fat1	UTSW	8	45,478,659 (GRCm39)	missense	probably damaging	0.99
R1482:Fat1	UTSW	8	45,406,281 (GRCm39)	missense	probably benign	0.04
R1496:Fat1	UTSW	8	45,486,427 (GRCm39)	missense	probably damaging	1.00
R1498:Fat1	UTSW	8	45,478,521 (GRCm39)	nonsense	probably null
R1508:Fat1	UTSW	8	45,479,899 (GRCm39)	missense	probably benign	0.01
R1577:Fat1	UTSW	8	45,476,420 (GRCm39)	missense	probably benign	0.30
R1646:Fat1	UTSW	8	45,471,079 (GRCm39)	missense	probably damaging	1.00
R1652:Fat1	UTSW	8	45,478,215 (GRCm39)	nonsense	probably null
R1656:Fat1	UTSW	8	45,478,567 (GRCm39)	nonsense	probably null
R1662:Fat1	UTSW	8	45,406,201 (GRCm39)	missense	probably benign	0.20
R1672:Fat1	UTSW	8	45,489,872 (GRCm39)	missense	probably damaging	1.00
R1704:Fat1	UTSW	8	45,478,613 (GRCm39)	missense	probably damaging	1.00
R1708:Fat1	UTSW	8	45,477,829 (GRCm39)	missense	probably damaging	1.00
R1710:Fat1	UTSW	8	45,463,519 (GRCm39)	missense	probably benign	0.00
R1812:Fat1	UTSW	8	45,489,840 (GRCm39)	missense	probably damaging	1.00
R1872:Fat1	UTSW	8	45,491,386 (GRCm39)	missense	probably damaging	1.00
R1872:Fat1	UTSW	8	45,406,341 (GRCm39)	missense	probably benign	0.01
R1883:Fat1	UTSW	8	45,504,184 (GRCm39)	missense	probably benign	0.17
R1893:Fat1	UTSW	8	45,476,893 (GRCm39)	missense	probably damaging	1.00
R1930:Fat1	UTSW	8	45,497,265 (GRCm39)	missense	possibly damaging	0.91
R1931:Fat1	UTSW	8	45,497,265 (GRCm39)	missense	possibly damaging	0.91
R1952:Fat1	UTSW	8	45,486,963 (GRCm39)	missense	probably benign	0.00
R1957:Fat1	UTSW	8	45,493,719 (GRCm39)	missense	probably damaging	1.00
R1999:Fat1	UTSW	8	45,405,430 (GRCm39)	missense	probably damaging	0.96
R2019:Fat1	UTSW	8	45,476,783 (GRCm39)	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45,479,741 (GRCm39)	missense	probably damaging	1.00
R2062:Fat1	UTSW	8	45,477,369 (GRCm39)	missense	probably damaging	1.00
R2117:Fat1	UTSW	8	45,490,500 (GRCm39)	missense	probably benign	0.33
R2196:Fat1	UTSW	8	45,477,683 (GRCm39)	missense	probably damaging	1.00
R2204:Fat1	UTSW	8	45,476,737 (GRCm39)	missense	probably damaging	1.00
R2256:Fat1	UTSW	8	45,403,408 (GRCm39)	missense	probably damaging	1.00
R2257:Fat1	UTSW	8	45,403,408 (GRCm39)	missense	probably damaging	1.00
R2409:Fat1	UTSW	8	45,493,567 (GRCm39)	splice site	probably benign
R2416:Fat1	UTSW	8	45,479,420 (GRCm39)	missense	probably damaging	1.00
R3021:Fat1	UTSW	8	45,497,048 (GRCm39)	missense	probably damaging	1.00
R3108:Fat1	UTSW	8	45,498,210 (GRCm39)	splice site	probably null
R3109:Fat1	UTSW	8	45,498,210 (GRCm39)	splice site	probably null
R3196:Fat1	UTSW	8	45,404,905 (GRCm39)	missense	probably benign	0.00
R3683:Fat1	UTSW	8	45,470,975 (GRCm39)	missense	probably benign
R3732:Fat1	UTSW	8	45,406,306 (GRCm39)	missense	possibly damaging	0.85
R3732:Fat1	UTSW	8	45,406,306 (GRCm39)	missense	possibly damaging	0.85
R3733:Fat1	UTSW	8	45,406,306 (GRCm39)	missense	possibly damaging	0.85
R3753:Fat1	UTSW	8	45,478,516 (GRCm39)	missense	probably damaging	0.97
R3905:Fat1	UTSW	8	45,476,072 (GRCm39)	missense	probably benign	0.00
R3907:Fat1	UTSW	8	45,476,072 (GRCm39)	missense	probably benign	0.00
R3908:Fat1	UTSW	8	45,476,072 (GRCm39)	missense	probably benign	0.00
R4060:Fat1	UTSW	8	45,478,518 (GRCm39)	missense	probably benign	0.09
R4061:Fat1	UTSW	8	45,478,518 (GRCm39)	missense	probably benign	0.09
R4062:Fat1	UTSW	8	45,478,518 (GRCm39)	missense	probably benign	0.09
R4063:Fat1	UTSW	8	45,478,518 (GRCm39)	missense	probably benign	0.09
R4078:Fat1	UTSW	8	45,442,159 (GRCm39)	missense	probably damaging	0.99
R4105:Fat1	UTSW	8	45,489,888 (GRCm39)	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45,503,981 (GRCm39)	missense	probably damaging	1.00
R4118:Fat1	UTSW	8	45,463,474 (GRCm39)	missense	probably damaging	1.00
R4161:Fat1	UTSW	8	45,489,824 (GRCm39)	missense	probably benign	0.00
R4364:Fat1	UTSW	8	45,405,999 (GRCm39)	missense	probably benign	0.01
R4394:Fat1	UTSW	8	45,405,383 (GRCm39)	missense	probably damaging	0.98
R4395:Fat1	UTSW	8	45,405,383 (GRCm39)	missense	probably damaging	0.98
R4396:Fat1	UTSW	8	45,405,383 (GRCm39)	missense	probably damaging	0.98
R4412:Fat1	UTSW	8	45,476,636 (GRCm39)	missense	probably damaging	0.99
R4542:Fat1	UTSW	8	45,494,931 (GRCm39)	missense	probably damaging	1.00
R4591:Fat1	UTSW	8	45,479,279 (GRCm39)	missense	probably benign
R4606:Fat1	UTSW	8	45,403,720 (GRCm39)	missense	possibly damaging	0.47
R4612:Fat1	UTSW	8	45,478,184 (GRCm39)	missense	probably damaging	1.00
R4730:Fat1	UTSW	8	45,486,514 (GRCm39)	missense	probably damaging	1.00
R4778:Fat1	UTSW	8	45,491,363 (GRCm39)	missense	probably benign	0.04
R4824:Fat1	UTSW	8	45,442,151 (GRCm39)	missense	probably damaging	1.00
R4829:Fat1	UTSW	8	45,489,199 (GRCm39)	missense	probably damaging	1.00
R4832:Fat1	UTSW	8	45,466,102 (GRCm39)	missense	possibly damaging	0.95
R4849:Fat1	UTSW	8	45,466,007 (GRCm39)	missense	probably benign	0.15
R4896:Fat1	UTSW	8	45,404,317 (GRCm39)	missense	possibly damaging	0.68
R4927:Fat1	UTSW	8	45,476,000 (GRCm39)	missense	probably damaging	0.96
R4941:Fat1	UTSW	8	45,489,312 (GRCm39)	missense	probably benign	0.00
R5011:Fat1	UTSW	8	45,484,300 (GRCm39)	critical splice acceptor site	probably null
R5040:Fat1	UTSW	8	45,476,417 (GRCm39)	missense	probably damaging	1.00
R5112:Fat1	UTSW	8	45,477,319 (GRCm39)	missense	probably damaging	1.00
R5151:Fat1	UTSW	8	45,404,851 (GRCm39)	missense	possibly damaging	0.74
R5161:Fat1	UTSW	8	45,405,549 (GRCm39)	missense	probably benign	0.00
R5162:Fat1	UTSW	8	45,478,846 (GRCm39)	missense	probably benign	0.02
R5353:Fat1	UTSW	8	45,489,168 (GRCm39)	missense	probably benign	0.13
R5425:Fat1	UTSW	8	45,478,922 (GRCm39)	missense	possibly damaging	0.64
R5458:Fat1	UTSW	8	45,466,090 (GRCm39)	missense	probably damaging	1.00
R5479:Fat1	UTSW	8	45,489,912 (GRCm39)	missense	possibly damaging	0.88
R5543:Fat1	UTSW	8	45,476,516 (GRCm39)	missense	probably damaging	0.99
R5569:Fat1	UTSW	8	45,492,873 (GRCm39)	missense	probably damaging	0.98
R5610:Fat1	UTSW	8	45,406,109 (GRCm39)	nonsense	probably null
R5734:Fat1	UTSW	8	45,504,246 (GRCm39)	missense	probably damaging	0.99
R5832:Fat1	UTSW	8	45,470,460 (GRCm39)	missense	possibly damaging	0.65
R5860:Fat1	UTSW	8	45,504,166 (GRCm39)	missense	probably benign
R5886:Fat1	UTSW	8	45,486,432 (GRCm39)	missense	probably damaging	1.00
R5886:Fat1	UTSW	8	45,480,718 (GRCm39)	critical splice donor site	probably null
R5919:Fat1	UTSW	8	45,479,910 (GRCm39)	critical splice donor site	probably null
R5930:Fat1	UTSW	8	45,497,073 (GRCm39)	missense	probably benign	0.10
R5960:Fat1	UTSW	8	45,486,405 (GRCm39)	missense	probably damaging	1.00
R5988:Fat1	UTSW	8	45,482,493 (GRCm39)	missense	probably benign	0.00
R6166:Fat1	UTSW	8	45,405,522 (GRCm39)	missense	probably damaging	1.00
R6184:Fat1	UTSW	8	45,406,429 (GRCm39)	missense	probably benign	0.00
R6208:Fat1	UTSW	8	45,480,650 (GRCm39)	missense	probably damaging	0.99
R6351:Fat1	UTSW	8	45,486,532 (GRCm39)	missense	probably damaging	1.00
R6391:Fat1	UTSW	8	45,405,379 (GRCm39)	missense	possibly damaging	0.69
R6701:Fat1	UTSW	8	45,403,718 (GRCm39)	missense	probably damaging	1.00
R6702:Fat1	UTSW	8	45,406,083 (GRCm39)	missense	probably benign	0.28
R6703:Fat1	UTSW	8	45,406,083 (GRCm39)	missense	probably benign	0.28
R6704:Fat1	UTSW	8	45,477,410 (GRCm39)	missense	probably damaging	1.00
R6822:Fat1	UTSW	8	45,479,441 (GRCm39)	missense	probably damaging	1.00
R6852:Fat1	UTSW	8	45,488,635 (GRCm39)	missense	possibly damaging	0.46
R6863:Fat1	UTSW	8	45,497,501 (GRCm39)	missense	probably damaging	1.00
R6885:Fat1	UTSW	8	45,405,489 (GRCm39)	missense	possibly damaging	0.94
R6912:Fat1	UTSW	8	45,504,060 (GRCm39)	missense	probably benign	0.00
R6927:Fat1	UTSW	8	45,477,532 (GRCm39)	missense	probably benign	0.41
R6964:Fat1	UTSW	8	45,496,982 (GRCm39)	missense	probably damaging	1.00
R7010:Fat1	UTSW	8	45,406,386 (GRCm39)	nonsense	probably null
R7062:Fat1	UTSW	8	45,403,253 (GRCm39)	start codon destroyed	probably null	0.99
R7063:Fat1	UTSW	8	45,493,812 (GRCm39)	missense	probably benign	0.09
R7071:Fat1	UTSW	8	45,442,145 (GRCm39)	missense	possibly damaging	0.67
R7117:Fat1	UTSW	8	45,484,505 (GRCm39)	missense	probably damaging	0.98
R7146:Fat1	UTSW	8	45,403,962 (GRCm39)	missense	probably benign
R7210:Fat1	UTSW	8	45,476,540 (GRCm39)	missense	probably damaging	1.00
R7227:Fat1	UTSW	8	45,463,646 (GRCm39)	missense	probably benign	0.08
R7270:Fat1	UTSW	8	45,490,475 (GRCm39)	missense	probably damaging	1.00
R7373:Fat1	UTSW	8	45,479,702 (GRCm39)	missense	probably damaging	1.00
R7390:Fat1	UTSW	8	45,405,511 (GRCm39)	missense	possibly damaging	0.81
R7465:Fat1	UTSW	8	45,497,189 (GRCm39)	missense	probably benign	0.35
R7476:Fat1	UTSW	8	45,484,311 (GRCm39)	missense	probably benign	0.01
R7483:Fat1	UTSW	8	45,476,197 (GRCm39)	missense	probably benign	0.13
R7484:Fat1	UTSW	8	45,489,221 (GRCm39)	missense	probably damaging	1.00
R7549:Fat1	UTSW	8	45,442,031 (GRCm39)	missense	probably benign	0.01
R7554:Fat1	UTSW	8	45,490,202 (GRCm39)	missense	possibly damaging	0.88
R7620:Fat1	UTSW	8	45,462,887 (GRCm39)	missense	possibly damaging	0.95
R7652:Fat1	UTSW	8	45,406,336 (GRCm39)	missense	probably damaging	1.00
R7694:Fat1	UTSW	8	45,441,967 (GRCm39)	critical splice acceptor site	probably null
R7746:Fat1	UTSW	8	45,404,670 (GRCm39)	missense	probably damaging	0.96
R7762:Fat1	UTSW	8	45,490,374 (GRCm39)	missense	probably damaging	0.99
R7762:Fat1	UTSW	8	45,476,359 (GRCm39)	missense	probably damaging	1.00
R7782:Fat1	UTSW	8	45,403,948 (GRCm39)	missense	probably damaging	1.00
R7801:Fat1	UTSW	8	45,495,260 (GRCm39)	missense	probably damaging	1.00
R7807:Fat1	UTSW	8	45,495,010 (GRCm39)	missense	probably damaging	1.00
R7821:Fat1	UTSW	8	45,403,261 (GRCm39)	missense	probably benign
R7869:Fat1	UTSW	8	45,504,259 (GRCm39)	missense	probably benign	0.02
R8034:Fat1	UTSW	8	45,404,728 (GRCm39)	missense	probably benign	0.28
R8094:Fat1	UTSW	8	45,405,739 (GRCm39)	missense	probably damaging	0.98
R8111:Fat1	UTSW	8	45,479,095 (GRCm39)	missense	possibly damaging	0.94
R8220:Fat1	UTSW	8	45,492,993 (GRCm39)	missense	probably null
R8221:Fat1	UTSW	8	45,406,390 (GRCm39)	missense
R8233:Fat1	UTSW	8	45,405,055 (GRCm39)	missense
R8250:Fat1	UTSW	8	45,406,336 (GRCm39)	missense	probably damaging	1.00
R8279:Fat1	UTSW	8	45,483,384 (GRCm39)	critical splice donor site	probably null
R8726:Fat1	UTSW	8	45,477,206 (GRCm39)	missense	probably benign	0.23
R8875:Fat1	UTSW	8	45,493,600 (GRCm39)	missense	probably damaging	1.00
R8937:Fat1	UTSW	8	45,483,350 (GRCm39)	missense	probably damaging	1.00
R8950:Fat1	UTSW	8	45,476,158 (GRCm39)	missense	probably damaging	1.00
R8971:Fat1	UTSW	8	45,495,331 (GRCm39)	missense	probably damaging	1.00
R8976:Fat1	UTSW	8	45,484,332 (GRCm39)	missense	probably benign	0.02
R9000:Fat1	UTSW	8	45,497,587 (GRCm39)	nonsense	probably null
R9032:Fat1	UTSW	8	45,492,894 (GRCm39)	missense	probably benign	0.01
R9076:Fat1	UTSW	8	45,492,938 (GRCm39)	missense	probably damaging	1.00
R9083:Fat1	UTSW	8	45,491,336 (GRCm39)	missense	probably benign	0.00
R9083:Fat1	UTSW	8	45,466,127 (GRCm39)	missense	possibly damaging	0.76
R9103:Fat1	UTSW	8	45,404,850 (GRCm39)	missense	probably benign	0.38
R9124:Fat1	UTSW	8	45,478,064 (GRCm39)	missense	possibly damaging	0.48
R9124:Fat1	UTSW	8	45,403,363 (GRCm39)	missense	probably benign
R9128:Fat1	UTSW	8	45,462,878 (GRCm39)	missense	probably benign	0.14
R9148:Fat1	UTSW	8	45,405,682 (GRCm39)	missense	possibly damaging	0.81
R9162:Fat1	UTSW	8	45,404,352 (GRCm39)	missense	probably damaging	1.00
R9209:Fat1	UTSW	8	45,404,791 (GRCm39)	missense	possibly damaging	0.80
R9276:Fat1	UTSW	8	45,488,514 (GRCm39)	missense	probably damaging	0.99
R9303:Fat1	UTSW	8	45,463,498 (GRCm39)	missense	probably damaging	1.00
R9319:Fat1	UTSW	8	45,406,060 (GRCm39)	missense	probably damaging	1.00
R9392:Fat1	UTSW	8	45,476,228 (GRCm39)	missense	probably damaging	1.00
R9616:Fat1	UTSW	8	45,406,075 (GRCm39)	missense	probably damaging	0.99
R9712:Fat1	UTSW	8	45,470,417 (GRCm39)	missense	probably benign	0.05
R9756:Fat1	UTSW	8	45,496,974 (GRCm39)	missense	probably damaging	0.96
RF001:Fat1	UTSW	8	45,442,003 (GRCm39)	missense	probably benign	0.00
X0064:Fat1	UTSW	8	45,478,771 (GRCm39)	missense	possibly damaging	0.58
Z1088:Fat1	UTSW	8	45,476,844 (GRCm39)	missense	possibly damaging	0.88
Z1176:Fat1	UTSW	8	45,489,875 (GRCm39)	missense	probably damaging	1.00
Z1176:Fat1	UTSW	8	45,476,633 (GRCm39)	missense	possibly damaging	0.65
Z1176:Fat1	UTSW	8	45,403,635 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACCAACATGGCCGGTTTGTC -3'
(R):5'- GTTGATGTCGATCACGTGCAC -3'

Sequencing Primer
(F):5'- GGTTTGTCCACCAACACAACGG -3'
(R):5'- GTGCACAGTCACATTCGCTG -3'

Posted On

2019-10-17